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Biocaster : Jurnal Kajian Biologi
Published by Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu
ISSN : 28083598     EISSN : 2808277X     DOI : https://doi.org/10.36312/biocaster
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Biocaster : Jurnal Kajian Biologi (B : JKB), focus to bridge the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of advances in biology research, teaching, and learning. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Biocaster : Jurnal Kajian Biologi (B : JKB), receiving and publishing article in the form of research and review (scientific article) in the field of biology education and utilization of biological research in teaching and learning. Each article must be linked to the theme "21st century skills in biology education" and / or "Efforts to support the achievements of the goals set forth in the Sustainable Development Goals (SDGs) through biology education". All articles are published in English and under go a peer-review process. The scope of Biocaster : Jurnal Kajian Biologi (B : JKB), is focused on biology education research/review both in topics covered as well as disciplinary perspective : 1) Biology teaching and learning materials at all education levels; 2) Pure Research of Biology developed or studied to the sources, materials, or instructional media Biology (using the Research and Development paradigm/R&D); 3) Curriculum of Biology Education at all education levels; 4) Quasi-experiment, Class Action Research (CAR), and Lesson Study in Biology teaching and learning; 5) Other Qualitative Research of Biology Education; 6) Biology Learning Evaluation/Assessment; 7) Teacher Professional Issues/Trends in Biology Education; and 8) Environmental education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 37 Documents
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Search results for , issue "Vol. 6 No. 2 (2026): April" : 37 Documents clear
Studi Etnobotani Ritual Erpangir Ku Lau pada Masyarakat Karo di Kecamatan Kabanjahe Kabupaten Karo Syahfitri, Dinda; Harsono, Tri; Suriani, Cicik; Sari, Wina Dyah Puspita; Handayani, Dina; Pulungan, Ahmad Shafwan S.
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1126

Abstract

The Erpangir Ku Lau ritual is a Karo people's self-purification ritual that has traditionally utilized plants as the main element. This study aims to examine the belief system (cosmos), local knowledge (corpus), and plant utilization practices (praxis) in the Erpangir Ku Lau ritual in Kabanjahe District. The study was conducted in January 2026 in Lau Cimba Village using a qualitative and quantitative descriptive approach through semi-structured interviews with 10 key informants selected by purposive sampling. The results identified 17 species from 11 plant families. The Rutaceae family dominated with 4 species (23.53%), followed by Rosaceae with 3 species (17.65%), and Poaceae with 2 species (11.76%). The most commonly utilized organs were fruits and flowers (29.41%), followed by leaves (23.53%). Shrub habitus dominated with 47.06%. The Karo people view plants as symbolic and spiritually significant, with the kaffir lime (Citrus hystrix D.C.) as a key plant. Plant use reflects holistic local wisdom, combining ecological, chemical, and spiritual aspects. Documenting this traditional knowledge is crucial to prevent its extinction due to modernization.
Resistensi Antibiotik Bakteri Escherichia coli dari Air Sumur di Peternakan Ayam Desa Suranadi, Lombok Barat Sopiandi, Sopiandi; Kholik, Kholik; Atma, Candra Dwi; Pradana, Munawer; Hendarti, Gracia Angelina
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1127

Abstract

Antimicrobial Resistance (AMR) has become a global health problem. Escherichia coli bacteria in water can act as a reservoir for the spread of antibiotic resistance. The study aimed to determine the resistance of Escherichia coli isolated from well water in chicken farms to antibiotics. The study design was an observational survey conducted from November to December 2022 at five chicken farms in Suranadi Village, Narmada District, West Lombok Regency. Five well water samples used in the study came from five wells in chicken farms located 10 meters from the chicken coop. 250 ml of well water samples were taken, put into sterile bottles, and then taken to the Health Laboratory Testing and Calibration Center of West Nusa Tenggara Province for isolation of Escherichia coli. Escherichia coli was isolated by planting on Eosin Methylene Blue Agar (EMBA) and identified using gram staining and biochemical tests. Antibiotic susceptibility testing of Escherichia coli was conducted using the disc diffusion method using five antibiotics: Gentamicin, Tetracycline, Amoxycillin, Chloramphenicol, and Ciprofloxacin. This study successfully isolated four Escherichia coli from five well water samples. The susceptibility test results showed that 75% of the Escherichia coli isolates were resistant to Amoxycillin, 50% to Ciprofloxacin, and 25% to Chloramphenicol. The Escherichia coli isolates were still sensitive to Gentamicin and Tetracycline.
Pengaruh Model Pembelajaran Guided Inquiry Berbantuan E-LKPD terhadap Kemampuan Berpikir Kritis dan Hasil Belajar Peserta Didik pada Materi Sistem Pernapasan di Kelas XI SMA Negeri 17 Samarinda Kristiani, Margaretha Dhea; Purwati, Sri; Herliani, Herliani; Lumowa, Sonja Verra Tinneke; Makkadafi, Suparno Putera
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1139

Abstract

This research is motivated by the lack of variety of learning models that cause students' critical thinking skills and learning outcomes to be still low. Therefore, the guided inquiry model assisted by E-LKPD is used as an alternative to facilitate the investigation process in a directed manner. This study aims to determine the effect of the guided inquiry learning model assisted by E-LKPD on students' critical thinking skills and learning outcomes in the respiratory system material of grade XI at SMA Negeri 17 Samarinda. This research is a quantitative study with a quasi-experimental type, using a non-equivalent control group design. Data collection techniques used pretest and posttest. The research sample consisted of 25 students per class. The experimental class used the guided inquiry model assisted by E-LKPD, while the control class used the Student Teams Achievement Divisions (STAD) model. The results of the t-test (independent sample t-test) showed sig. (2-tailed) of 0.000 < 0.05 and tcount > ttable, namely 4.448 ˃ 2.010 for critical thinking skills, and 4.903 ˃ 2.010 for student learning outcomes. The results of the study indicate that the guided inquiry learning model assisted by E-LKPD has a significant effect on improving critical thinking skills and student learning outcomes in the respiratory system material.
Pemanfaatan Tumbuhan Obat Berpotensi Tinggi dalam Tradisi Oukup dengan Studi Index of Cultural Significance pada Masyarakat Karo di Kecamatan Kabanjahe Kabupaten Karo Febrianti, Anastasiya Octavia; Harsono, Tri; Suriani, Cicik; Hasairin, Ashar; Nasution, Muhammad Yusuf; Pranoto, Hendro
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1142

Abstract

This research is a descriptive mixed-method ethnobotanical study aimed at identifying medicinal plants in the Oukup tradition of the Karo people based on the Index of Cultural Significance (ICS), analyzing utilization patterns, and analyzing community perspectives on their benefits and effectiveness. The research was conducted in Kabanjahe District through interviews and observations using a snowball sampling technique with 10 informants. Sixteen medicinal plant species were recorded, with the highest ICS score being ginger (50), followed by pepper (40), sappanwood (24), black ginger (20), and torch ginger (18). Plants are used in the form of rhizomes, stems, leaves, and fruits through boiling and steaming for postpartum recovery, increasing stamina, relaxing, warming the body, and reducing aches. The community positively assesses Oukup in terms of benefits, safety, and effectiveness, thus maintaining its relevance in the traditional health system and having the potential to be developed and preserved.
Analisis Tingkat Pemahaman Siswa pada Materi Perubahan dan Pelestarian Lingkungan di Kelas X SMA Negeri 1 Tilango Bau, Fadila; Hasan, Ani M.; Lamangantjo, Chairunnisah J.
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1155

Abstract

This study aims to analyze the level of student understanding of environmental change and conservation in grade X of SMA Negeri 1 Tilango. The study used a descriptive quantitative approach, involving 25 grade X3 students. The method used was a multiple-choice test based on seven indicators of understanding: identifying, explaining, interpreting, classifying, comparing, summarizing, and understanding. The results showed that all indicators improved from the pretest, which was generally in the low category, to the posttest, which reached an average of 77.81%, categorized as high. This improvement reflects that the applied learning process was able to improve students' conceptual mastery of environmental change and conservation, while simultaneously strengthening their analytical thinking skills. A comparison of pretest and posttest scores indicates that the instrument used was effective in measuring students' conceptual understanding and that the applied learning positively influenced learning outcomes.
Pengaruh Media Interaktif Genially Berbantuan Model Discovery Learning terhadap Hasil Belajar Kognitif pada Materi Sistem Saraf Siswa Kelas XI SMA Negeri 5 Samarinda Davanya, Dinda; Purwati, Sri; Lumowa, Sonja V. T.; Makkadafi, Suparno Putera; Serena, Nelda Anasthasia
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1162

Abstract

This study aims to determine the effect of Genially interactive media supported by the discovery learning model on students' cognitive learning outcomes in the nervous system. This research is motivated by the low level of student active engagement in learning, necessitating innovative media and learning models that support meaningful conceptual understanding. This study used a quasi-experimental method. The subjects consisted of an experimental class using Genially media supported by the discovery learning model and a control class using the expository model. The study was conducted on eleventh-grade students of SMA Negeri 5 Samarinda on the nervous system. The research instrument was an essay test administered before and after the lesson. Data analysis included normality tests, homogeneity tests, hypothesis testing using the independent sample t-test, and N-gain calculations. The analysis results showed that the data were normally distributed and homogeneous. The hypothesis test revealed a significant difference between cognitive learning outcomes in the experimental and control classes. The improvement in cognitive learning outcomes in the experimental class was in the moderate category and higher than in the control class. Thus, Genially interactive media supported by the discovery learning model is effective in improving students' cognitive learning outcomes in the nervous system.
Pengaruh Konsentrasi Ekstrak Bawang Merah (Allium cepa L.) sebagai Zat Pengatur Tumbuh terhadap Pertumbuhan Krisan (Chrysanthemum morifolium) Secara In Vitro Ridwan, Radhika Nabila Ma’rufi; Edi, Syahmi
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1170

Abstract

Chrysanthemum (Chrysanthemum morifolium) is a major ornamental plant commodity in Indonesia with a production level of up to 459 million stems. The success of in vitro propagation is largely determined by the use of appropriate Plant Growth Regulators (PGRs). This study aimed to evaluate the performance of shallot (Allium cepa L.) extract as a natural PGR, while determining the optimal dose to support chrysanthemum growth in vitro. The study was conducted at the Laboratory of the Horticulture Main Center (BIH) Gedung Johor, Medan, from October 2025 to January 2026, using a Completely Randomized Design (CRD) that included six concentration levels (K0=0; K1=5; K2=10; K3=15; K4=20; and K5=25 mL L⁻¹), with 10 replications. Data analysis was performed using ANOVA-DMRT for parametric data and Kruskal-Wallis–Dunn for non-parametric data. The study results indicated a significant effect (p < 0.01) on all growth parameters. A concentration of 20 mL L⁻¹ was most effective for organogenesis, producing 15.60 leaves, 7.85 roots, and 3.15 shoots; while 5 mL L⁻¹ was superior for elongation, with plantlets reaching 8.99 cm in height. Concentrations above the optimal dose actually inhibited growth due to the accumulation of phytotoxic compounds. These findings provide a more cost-effective and sustainable chrysanthemum propagation protocol as a substitute for synthetic plant growth regulators.
Gambaran Hasil Pemeriksaan Pewarnaan Diff-Quik pada Swab Mukosa PSK Hermawan, Rizal Aditya; Sabban, Indra Fauzi; Angelina, Lisa; Kusnidar, Gilang; Wahyuni, Ismiy Noer; Rokim, Moch. Abdul
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1173

Abstract

High-risk commercial sex workers (CSWs) are highly susceptible to sexually transmitted infections (STIs), which often manifest in the oral mucosa. Therefore, a rapid, accurate, and affordable oral health screening method at the primary healthcare level is needed to detect cellular changes early. The purpose of this study was to describe the morphology of epithelial cells and the quality of oral mucosal swabs obtained using Diff-Quik staining in CSWs. This study used a descriptive observational study of 20 CSW respondents, with a total of 80 samples taken using the buccal mucosal swab technique and stained using the Diff-Quik method. The results showed that 81.25% of the samples had good staining quality, with clear nuclear and cytoplasmic details. Although the cell morphology was generally normal, pathological findings in the form of cellular damage (vacuolization and pyknosis) and inflammatory cell infiltration (PMN leukocytes) were found in 27.5% of the samples. The conclusion of this study is that Diff-Quick staining is very effective for rapidly assessing oral cytology, which indicates inflammation and cell damage in CSWs in the region.
Analisis Total Bakteri Coliform di Perairan Pantai Liang Kabupaten Maluku Tengah Sirajuddin, Nur Tasmiah; Mamesah, Juliaeta A. B.
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1182

Abstract

Coastal waters are widely used for various human activities, including tourism. Liang Beach in Central Maluku Regency is one of the coastal tourist destinations experiencing environmental pressure due to increased population activity that has the potential to cause nutrient enrichment that can increase the number of coliform bacteria as an indicator of microbiological pollution. This study aims to analyze the number of coliform bacteria in the waters of Liang Beach and compare it with the applicable water quality threshold. This study used a purposive sampling method divided into 3 sampling points with 3 replications each, the distance of each point from the coastline is 5 meters, 7 meters, and 10 meters. Analysis of the number of coliform bacteria was carried out using standard microbiological methods and expressed in units of CFU/100 ml. The results showed that the average total coliform bacteria at point 1 was 100 CFU/100 ml, point 2 was 128.7 CFU/100 ml, and point 3 was 94.7 CFU/100 ml. These results indicate that Liang Beach still meets microbiological quality standards and is relatively safe for tourism activities. This research is expected to provide baseline data for microbiological research and sustainable management in the waters of Liang Beach.
Analisis Molekuler Keberadaan Patogen Malaria pada Sampel Klinis dari Labkesda Aceh Barat Berbasis Real-Time PCR Aisyah, Siti; Sardi, Arif; Hayatillah, Raudah; Nur, Abidah; Wilya, Veny
Biocaster : Jurnal Kajian Biologi Vol. 6 No. 2 (2026): April
Publisher : Lembaga Pendidikan, Penelitian, dan Pengabdian Kamandanu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/biocaster.v6i2.1200

Abstract

Malaria remains a public health problem in pre-elimination areas, primarily due to the increase in cases with low parasitemia, which often go undetected by microscopic examination and potentially maintain hidden transmission. This study aims to identify the presence of malaria pathogens in clinical samples from the West Aceh Regional Health Laboratory (Labkesda) using real-time Polymerase Chain Reaction (qPCR) as a molecular confirmation approach. This study employed a quantitative descriptive design based on diagnostic surveillance. Dried Blood Spot (DBS) samples from patients exhibiting malaria symptoms were analyzed at the Banda Aceh Public Health Laboratory (Labkesmas). The results showed that four of the five samples tested positive for Plasmodium, and all were identified as Plasmodium knowlesi with Ct values ​​<40. This finding indicates the presence of malaria infections that could potentially go undetected by conventional microscopy. Thus, qPCR has the potential to be a sensitive confirmation method to support malaria case detection in surveillance activities.

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