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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 12 Documents

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Search results for , issue "Vol 17, No 2 (2023): June" : 12 Documents clear

EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER Fitrine Ekawasti; Zul Azmi; Didik Tulus Subekti; muhammad ibrahim desem; Arifin Budiman Nugraha; Siti Sa’diah; Umi Cahyaningsih
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.22251

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that stored on liquid nitrogen was isolated using DNAzol™ reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.

PHYSIOLOGICAL RESPONSES OF FEMALE BEEF CATTLE AGAINST PEATLAND MICROCLIMATE STRESS IN CENTRAL KALIMANTAN Adrial Adrial; Rudy Priyanto; Salundik Salundik; Ahmad Yani; Luki Abdullah
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.29115

This study aimed to evaluate the physiological response of female beef cattle to peatland microclimate stress in Central Kalimantan. This study used direct observation on small holder beef cattle farm. Microclimate data on 41 units cattle barns and physiological parameters of female cattle were collected in the morning, at noon and in the afternoon. The physiological parameter measurements involved 215 female beef cattle, consisting of 119 Bali and 96 crossbred cattle with different physiological stages including pregnant cows, lactating cows, dry cows, heifers and calves. The microclimate condition within cattle barns on peatland of Central Kalimantan is not the comfort zone for beef cattle. It is characterized by high air temperature and relative humidity, and low wind speed, which result in high temperature humidity index (THI). The barn with gable roof type and asbestos materials gave the lowest THI. This Microclimate caused heat stress to beef cattle reared on the peatlands, indicated by the high rectal temperature, heart rate, respiratory rate, and heat tolerance coefficient, although it was still categorized as mild to moderate stress. Bali cattle showed better physiological responses to microclimate stress than crossbred cattle. Pregnant crossbred cows were the most susceptible to peatland microclimate stress.

THE EFFECT OF AGE ON WOUND HEALING AND PHYSICAL DEVELOPMENT OF LOCAL CATS (Felis catus) POST-OVARIOHYSTERECTOMY SURGERY Renji Mailisa Wahyuni; G Gunanti; Deni Noviana
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.28242

The purpose of this study was to evaluate the effect of age on wound healing and physical development of local cats (Felis catus) after ovariohysterectomy. A total of 15 female cats were divided into three age groups (2-4, 5-9, and 10 months old) with each group consisting of five cats. The treatment group was sterilized using the lateral flank ovariohysterectomy method. Observations of surgical procedures and physical conditions were carried out during anesthesia, including onset, duration of anesthesia and surgery, body temperature, heart rate, and respiratory rate. Post-operative observations were made to evaluate wound healing, hematological profile (RBC, Hb, PCV, WBC, PLT), body weight, Body Condition Score (BCS), bone density, and growth plate closure. The results showed that there was no significant difference (P0.05) during anesthesia, wound healing, or an increase in body weight, BCS, and bone density in all age groups. Ovariohysterectomy at 2-4 months old delayed the closure of the bone growth plates (P0.05). Ovariohysterectomy in pubertal and post-pubertal cats is safe to perform as long as good surgical preparation is provided.

BOER BUCK SPERM QUALITY IN EXTENDER SUPPLEMETED WITH NON-ENZYMATIC ANTIOXIDANTS AND STORAGE AT COLD TEMPERATURE Lalu Zaenuri; R Rodiah; Adji Santoso Dradjat; Lukman HY; Eny Yuliani
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.26913

The aim of this study was to determine the durability of standard extender tris egg yolk supplemented with non-enzymatic antioxidants from guava and fig fruit filtrate on the motility and viability of Boer buck sperm. Semen was collected using an artificial vagina every four days for ten replications. The treatment extenders were Tris+2.5% egg yolk (P0) and P0+1.487 mg vitamin A and P0+3.958 mg vitamin C per ml/100 ml (v/v) hereinafter referred as P1 and P2, respectively. The experimental design was a completely randomized 3 times 10 a factorial pattern. The variables assessed were individual motility, progessive motility and viability of sperm evaluated every day for 4 consecutive days. Result indicated that, 4 days after extended and stored at 5°C, the average individual motility, progessive motility and sperm viability were 45.2±7.94; 33.0±10.2 and 49.6±7.69 for P1, and 45.4±6.14; 30.4±8.38 and 50.5±5.88 for P2. The data on the three indicators of sperm quality between P1 and P2 did not show a significant difference (P0.05). While for P0 30.6±5.85; 24.6±7.30 and 40.7±5.77, respectively, and were significantly lower (P 0.05) than P0 or P1. It was concluded that semen extended using tris-egg yolk extender supplemented with vitamin A or C could still be used for Artificial Insemination until day 4 after extended and storage at 5°C. It is suggested that research needs to be done to produce a ready to use, simple preaparation, cheap and long lasting instant extender.

DETERMINATION OF OVULATION TIME IN GAYO MARES BASED ON IMAGE OF PREOVULATORY FOLLICLE GROWTH Juli Melia; A Amrozi; Iman Supriatna; Muhammad Agil
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.26766

This study aimed to obtain the ultrasound image of preovulatory follicle growth in gayo mares to predict the ovulation time. This study used three Gayo mares and one gayo stallion for teaser aged 5-10 years and weighed 200-250 kg. Preovulatory follicle growth was observed using transrectal ultrasonography which was performed when the dominant follicle size was ≥ 3.5 cm. Observations were made for 3 consecutive estrous periods on gayo mares at relatively the same time. The absence of previous follicular appearance and the presence of hypoechoic area in the same ovary indicate that ovulation has occurred. The result revealed that the diameter of preovulatory follicle of gayo mares was 4.02±0.32 to 5.05±0.04 cm. There was no significant follicle growth in the last 48 hours before ovulation and the preovulatory follicle shape altered from spherical to pearshape. Many small follicles (2 cm in diameter) were observed in peripheral area of the ovary indicated the recruitment of follicles which form the first follicular wave. To conclude, the alteration in the size and pearshape of preovulatory follicle and the recruitment of small follicles can be used as a marker to determine the time of ovulation on gayo mares.

THE EFFECT OF WHOLE SEED (Barringtonia racemosa) WATER EXTRACT ON ERYTHROCYTE, HEMOGLOBIN AND HEMATOCRITE COUNT OF WHITE RAT (Rattus norvegicus) EXPOSED TO CIGARETTE SMOKE D Dasrul; Yayang Nuri Al Aliya; Amalia Sutriana; Nuzul Asmilia; Razali Daud
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.25424

This study aims to determine the effect of Barringtonia racemosa seed extract on the number of erythrocytes, hemoglobin levels, and the percentage of hematocrit in white rats (Rattus norvegicus) exposed to cigarette smoke. A total of 25 white rats Wistar strain, aged 3-4 months, male, weighing 180-200 g, were randomly divided into 5 treatment groups, each group consisting of 5 rats. Positive control group (KP) without exposure to cigarette smoke and without administration of Barringtonia racemosa seed extract, negative control group (KN) exposed to cigarette smoke without administration of Barringtonia racemosa seed extract. Treatment groups P1, P2, P3 were exposed to cigarette smoke and given Barringtonia racemosa seeds with doses of 50, 100, and 150 mg/kg BW/day, respectively. The extract was administered orally for 30 days. Blood collection in all groups was carried out via the orbital vein using a hematocrit pipette after the rats had been sedated with 0.2 mL of ketamine. Erythrocyte, hemoglobin, and hematocrit values were calculated using a hematology analyzer. Data were analyzed using one-way pattern analysis of variance (ANOVA). The results showed that giving Barringtonia racemosa seeds at doses of 50, 100 and 150 mg/kg/day had a significant effect (P0.05) in increasing the values of erythrocytes, hemoglobin, and hematocrit of white rats exposed to cigarette smoke compared to the control group which was not given the extract. In conclusion, the administration of Barringtonia racemosa seed extract can increase the number of erythrocytes, hemoglobin levels, and the percentage of hematocrit exposed to cigarette smoke.

DETERMINATION OF OVULATION TIME IN GAYO MARES BASED ON IMAGE OF PREOVULATORY FOLLICLE GROWTH Melia, Juli; Amrozi, A; Supriatna, Iman; Agil, Muhammad
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.26766

This study aimed to obtain the ultrasound image of preovulatory follicle growth in gayo mares to predict the ovulation time. This study used three Gayo mares and one gayo stallion for teaser aged 5-10 years and weighed 200-250 kg. Preovulatory follicle growth was observed using transrectal ultrasonography which was performed when the dominant follicle size was 3.5 cm. Observations were made for 3 consecutive estrous periods on gayo mares at relatively the same time. The absence of previous follicular appearance and the presence of hypoechoic area in the same ovary indicate that ovulation has occurred. The result revealed that the diameter of preovulatory follicle of gayo mares was 4.020.32 to 5.050.04 cm. There was no significant follicle growth in the last 48 hours before ovulation and the preovulatory follicle shape altered from spherical to pearshape. Many small follicles (2 cm in diameter) were observed in peripheral area of the ovary indicated the recruitment of follicles which form the first follicular wave. To conclude, the alteration in the size and pearshape of preovulatory follicle and the recruitment of small follicles can be used as a marker to determine the time of ovulation on gayo mares.

THE EFFECT OF AGE ON WOUND HEALING AND PHYSICAL DEVELOPMENT OF LOCAL CATS (Felis catus) POST-OVARIOHYSTERECTOMY SURGERY Wahyuni, Renji Mailisa; Gunanti, G; Noviana, Deni
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.28242

The purpose of this study was to evaluate the effect of age on wound healing and physical development of local cats (Felis catus) after ovariohysterectomy. A total of 15 female cats were divided into three age groups (2-4, 5-9, and 10 months old) with each group consisting of five cats. Thetreatment group was sterilized using the lateral flank ovariohysterectomy method. Observations of surgical procedures and physical conditions were carriedout during anesthesia, including onset, duration of anesthesia and surgery, body temperature, heart rate, and respiratory rate. Post-operative observationswere made to evaluate wound healing, hematological profile (RBC, Hb, PCV, WBC, PLT), body weight, Body Condition Score (BCS), bone density, andgrowth plate closure. The results showed that there was no significant difference (P0.05) during anesthesia, wound healing, or an increase in body weight,BCS, and bone density in all age groups. Ovariohysterectomy at 2-4 months old delayed the closure of the bone growth plates (P0.05).Ovariohysterectomy in pubertal and post-pubertal cats is safe to perform as long as good surgical preparation is provided.

THE EFFECT OF WHOLE SEED (Barringtonia racemosa) WATER EXTRACT ON ERYTHROCYTE, HEMOGLOBIN AND HEMATOCRITE COUNT OF WHITE RAT (Rattus norvegicus) EXPOSED TO CIGARETTE SMOKE Dasrul, D; Aliya, Yayang Nuri Al; Sutriana, Amalia; Asmilia, Nuzul; Daud, Razali
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.25424

BOER BUCK SPERM QUALITY IN EXTENDER SUPPLEMETED WITH NON-ENZYMATIC ANTIOXIDANTS AND STORAGE AT COLD TEMPERATURE Zaenuri, Lalu; Rodiah, R; Dradjat, Adji Santoso; HY, Lukman; Yuliani, Eny
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.26913

The aim of this study was to determine the durability of standard extender tris egg yolk supplemented with non-enzymatic antioxidants from guava and fig fruit filtrate on the motility and viability of Boer buck sperm. Semen was collected using an artificial vagina every four days for ten replications. The treatment extenders were Tris+2.5% egg yolk (P0) and P0+1.487 mg vitamin A and P0+3.958 mg vitamin C per ml/100 ml (v/v) hereinafter referred as P1 and P2, respectively. The experimental design was a completely randomized 3 times 10 a factorial pattern. The variables assessed were individual motility, progessive motility and viability of sperm evaluated every day for 4 consecutive days. Result indicated that, 4 days after extended and stored at 5C, the average individual motility, progessive motility and sperm viability were 45.27.94; 33.010.2 and 49.67.69 for P1, and 45.46.14; 30.48.38 and 50.55.88 for P2. The data on the three indicators of sperm quality between P1 and P2 did not show a significant difference (P0.05). While for P0 30.65.85; 24.67.30 and 40.75.77, respectively, and were significantly lower (P 0.05) than P0 or P1. It was concluded that semen extended using tris-egg yolk extender supplemented with vitamin A or C could still be used for Artificial Insemination until day 4 after extended and storage at 5C. It is suggested that research needs to be done to produce a ready to use, simple preaparation, cheap and long lasting instant extender.

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Tongku Nizwan Siregar

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+626517551536

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jkh@usk.ac.id

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Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Kab. aceh besar,

Aceh

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Tongku Nizwan Siregar

Contact Email jkh@usk.ac.id

Phone +626517551536

Journal Mail Official jkh@usk.ac.id

Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location Kab. aceh besar, Aceh INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA