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Current Biomedicine
Published by Institut Pertanian Bogor
ISSN : 29628490     EISSN : 29854784     DOI : https://doi.org/10.29244/currbiomed
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
Aim. Current Biomedicine aims to publish scientific article in the biomedical fields. Scope. Current Biomedicine will publish widely relevant topic in the field of biology (life science), directly or indirectly, support the improvement of human health. These fields include, but are not limited to: anatomy, physiology, histology, embryology, genetics, pharmacology, toxicology, pharmacy, parasitology, pathology, microbiology, clinics, radiology and imaging, surgery, experimental surgery, reproduction, ethnomedicine, phytopharmaceuticals, biotechnology, biomedical engineering, bioinformatics, public health, epidemiology, legislation and bioethics, and one health.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 54 Documents
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Effect of platelet-rich plasma on fibroblasts induced by lipopolysaccharide: in vitro study for wound healing Idris, Muthoharoh; Kusuma, Indra; Juniarti, Juniarti
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.36-44

Abstract

Platelet-rich plasma (PRP) is expected to be an alternative therapy in wound healing by accelerating the inflammatory process and increasing the healing factors so that the healing process or inflammation is faster. The purpose of this study was to examine the effect of calcium chloride (CaCl2)-activated PRP on the viability, migration, and cytokine levels of interleukin-6 (IL-6) and vascular endothelial growth factor (VEGF) of dermal fibroblast cells in lipopolysaccharide (LPS)-induced inflammatory conditions, as a model in wound healing in vitro. Fibroblast cells were grown in DMEM medium induced with LPS, and then CaCl2-activated PRP treatment was added. Measure fibroblast cell viability using CCK-8 kit (cell counting kit-8) was evaluated using a microplate reader, and the cell migration was evaluated using scratch-assay and TScratch software. Expression of IL-6 and VEGF using ELISA kit. All data were analyzed using software SPSS version 26 by performing a one-way analysis of variance (ANOVA), Kruskal Wills, and Mann-Whitney tests. The results showed that PRP significantly increased fibroblast cell viability in the 10% PRP treatment group. This study shows that PRP does not reduce IL-6 cytokine levels but can increase VEGF growth factor in fibroblast cell cultures. PRP increased cell migration so that the healing process was faster. In conclusion, the CaCl2-activated PRP on LPS-induced fibroblast cells can increase viability and accelerate cell migration; it can’t decrease IL-6 but can increase VEGF expression. PRP is expected to be an alternative therapy in wound healing.
Resistansi Escherichia coli patogen asal ayam petelur terhadap beberapa antibiotik Suhendi, Adnan Rizal; Afiff, Usamah; Handharyani, Ekowati
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.45-54

Abstract

Latar Belakang: Penggunaan antibiotik yang tidak tepat pada ternak menyebabkan munculnya resistansi bakteri terhadap antibiotik. Salah satu bakteri yang telah dilaporkan resistan adalah Escherichia coli sehingga pengobatan untuk infeksi bakteri ini menjadi kurang efektif. Tujuan: Penelitian ini bertujuan untuk mendeskripsikan resistansi E. coli patogen yang berasal dari usap kloaka ayam petelur terhadap antibiotik. Metode: Sebanyak 20 sampel usap kloaka diidentifikasi keberadaan E. coli melalui pengamatan makroskopis, mikroskopis, uji biokimia, dan fermentasi karbohidrat uji patogenisitas dilakukan dengan menggunakan media Congo red. Isolat yang patogen selanjutnya diuji kepekaannya terhadap 6 jenis antibiotik dengan uji kepekaan difusi cakram Kirby-Bauer. Hasil: Penelitian memperoleh 15 isolat E. coli dan uji Congo red mendapatkan 4 isolat E. coli patogen. Hasil penelitian menunjukkan bahwa 75% isolat resistan terhadap azitromisin, 100% isolat resistan intermediet terhadap doksisiklin dan siprofloksasin. Sementara itu, 75% isolat masih sensitif terhadap gentamisin dan 100% isolat sensitif terhadap kloramfenikol dan amoksisilin. Kesimpulan: Penelitian ini berhasil mengisolasi E. coli yang bersifat patogen dari ayam petelur yang telah resistan terhadap beberapa antibiotik yang diujikan.
Sinergisme aktivitas antibakteri ekstrak biji bengkuang (Pachyrhizus erosus L.) terhadap Pseudomonas aeruginosa berdasarkan bioautografi Rahminiwati, Min; Supardi, Arviani Rahmawati; Mahyuni, Siti
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.1-12

Abstract

Background: A 70% ethanol extract of jicama seeds has been reported to have antibacterial activity against Pseudomonas aeruginosa. Phytochemical tests of the extract have identified alkaloids, flavonoids, saponins, and tannins. However, the compounds responsible for its antibacterial effects against P. aeruginosa have not yet been studied. Objective: This study was conducted to determine the class of chemical compounds responsible for the antibacterial activity against P. aeruginosa. Methods: The antibacterial activity against P. aeruginosa was studied using the minimum bactericidal concentration (MBC) of the jicama seed extract at concentrations of 6, 10, 14, 18, 22, 26, 30, and 34% in liquid and solid Nutrient Agar media, the wide inhibitory zone (WIZ) using the Kirby Bauer method, and the retention factor (Rf) of the extract using thin-layer chromatography (TLC)-bioautography. Results: The study results showed that the jicama seed extract had an MBC value of 26%, and the best WIZ value in the extract was 5.68 mm at concentration of 34%. The Rf value in the extract at concentration of 34% which showed antibacterial activity against P. aeruginosa was 0.812, 0.750, 0.675, 0.625, and 0.787 of each compound group identified as flavonoids, tannins, quinones, alkaloids, and triterpenoids. The WIZ value of each compound was lower than the WIZ value of the extract. Conclusion: Based on the Rf data, the antibacterial effect of jicama seed extract against P. aeruginosa is likely the result of a synergistic interaction between the chemical compounds contained therein.
Optimasi suhu amplifikasi DNA pada quantitative polymerase chain reaction untuk identifikasi Mycobcterium tuberculosis resistan isoniazid Endarwati, Dwi Veni; Indra, Asep Iin Nur; Hardiana, Acep Tantan; Abror, Yogi Khoirul; Nurhayati, Betty; Merdekawati, Fusvita
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.61-70

Abstract

Background: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis and is a serious threat to global health. The methods can be used to detect and identify the bacteria is quantitative polymerase chain reaction (qPCR). In this method, denaturation and extension temperatures are determining factors of success that needs to be optimized. Objective: This study aims to optimize denaturation and extension temperatures in M. tuberculosis DNA amplification. Methods: The research used quasi-experimental design. The denaturation temperature optimized were 93, 94, 95, 96, and 97°C, and the extension temperature optimized were 58, 59, 60, 61, and 62°C. The test sample was a 1 ml sputum sample isolated from a patient with isoniazid-resistant M. tuberculosis. Optimization was performed using seven test primers, namely S315T, S315N, S315I, S315R, S315G, S315L, and R463B with the katG gene target and data analysis using Ms Excel. Data optimization results were processed with Excel by taking the lowest Ct value. Results: The results showed that the optimization temperatures for denaturation were different for each primer used. Primers S315T, S315R, and S315G, optimal with denaturation temperature of 96°C, primer S315N optimal with 94°C, primers S315I and R463B optimal with 93°C, and for primer S315L optimal with 95°C, with the most widely used temperature is 96°C. The optimal extension temperature was 58°C for primers S315T, S315N, S315I, and R463B, at 60°C for primers S315R and S315G, and at 61°C for primer S315L. Conclusion: The optimal denaturation temperature in this study was 96°C and the optimal extension temperature was 58°C.
Konsentrasi dan kemurnian ekstraksi DNA metode sonikasi dan spin column dari sampel dahak penderita tuberkulosis Saputra, Fitrianingsih; Indra, Asep Iin Nur; Djuminar, Ai; Merdekawati, Fusvita; Nurhayati, Betty
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.84-92

Abstract

Background: The Polymerase Chain Reaction (PCR) method can identify Mycobacterium tuberculosis in a sputum sample of a patient with TB (TB). One crucial step to ensure accurate PCR results is the DNA extraction process. Objective: The research aims to compare the concentration and purity of DNA from the sputum of TB patients using ultrasound and spin column extraction techniques. Methods: The research uses descriptive study designs with post-only design strategies. The primary data was derived from 18 sputum specimens from TB patients. Concentration measurement and DNA purity testing using a nanodrop spectroscopic photometer. Results: DNA extraction by ultrasound method has an average concentration of 18.9 ± 8.5 ng/L, with a peak of 37.6 ng/ L. The spin column method produces an average of 55.5 ± 27.9 ng/μL; the peak is 105.0 ng/ μL. The purity value of the DNA extract is in the range of 1.8 ± 2.0 with the ultrasound method of 61% and the spin column of 78%. Conclusion: The sonication method has a lower average concentration and a higher percentage of purity than the spin column method, and there are differences in concentrations and purity values between the two methods.
Efek penggunaan ulang larutan fiksatif formalin pada kualitas preparat histopatologi dan jumlah limbah yang dihasilkan Hardi, Zon; Wiryanti, Wiwin; Durachim, Adang; Rahmat, Mamat
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.71-83

Abstract

Background: Neutral buffered formalin (NBF) 10% fixative solution is widely used in histopathological slides. The fixation process generates liquid waste of NBF 10% and solid waste of tissue remnants. Objective: The research aimed to assess the reuse of NBF 10% fixative solution on the quality of histopathological slides and calculate the amount of waste produced. Methods: Treatments included single-use of fixative solution (control), reuse for 1, 2, and 3 times. Ten sample slides were prepared for each treatment, consisting of intestinal tissue, uterine fibroids, prostate, uterus, ovarian cyst, portio vaginalis cervicis, thyroid, rectum, breast fibroadenoma, and gallbladder tissues. Tissues were fixed with NBF 10% and processed histologically with hematoxylin-eosin staining. Liquid waste of NBF 10% and solid waste of tissue remnants were quantified. Histopathological slide quality was measured under a microscope for nuclear and cytoplasmic clarity, staining intensity, and color uniformity. Results: Control slides exhibited good quality with clearly blue-stained nuclei, pink cytoplasm, no color accumulation, and uniform staining across fields of view. Reused NBF 10% slides experienced a decrease in quality compared to the control but were still usable for diagnosis. Slides reused 2 and 3 times showed poor quality, making diagnosis difficult. Fixation resulted in 299.0 liters of liquid waste of NBF 10% and 64.9 kilograms of solid tissue remnants. Conclusion: Reusing NBF 10% decreases histological slide quality, though reuse once still allows for diagnosis. Reusing 10% NBF for tissue fixation can reduce the liquid waste of fixative solution and solid tissue waste.
Front Matter Vol 2 No 1 Biomedicine, Current
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.i-iii

Abstract

https://doi.org/10.29244/currbiomed.2.1.i-iii
Back Matter Vol 2 No 1 Biomedicine, Current
Current Biomedicine Vol. 2 No. 1 (2024): January
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.1.v-x

Abstract

Hipertrofi sel goblet usus halus ayam kampung asal pasar tradisional Jakarta yang terinfeksi cacing cestoda Jerica, Monica Silva; Tiuria, Risa; Mayasari, Ni Luh Putu Ika; Nugraha, Arifin Budiman; Subangkit, Mawar
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.93-100

Abstract

Latar Belakang: Ayam kampung merupakan salah satu kebutuhan protein hewani yang sering dicari masyarakat. Pemeliharaan ayam kampung dengan sistem dibiarkan lepas menjadi predisposisi terinfeksi oleh parasit gastrointestinal. Tujuan: Penelitian ini bertujuan mengkaji perubahan sel epitel dan hipertrofi sel goblet pada saluran pencernaan ayam kampung yang terinfeksi cacing cestoda Raillietina spp. secara alami. Metode: Penelitian ini menggunakan tujuh preparat arsip histopatologi usus halus ayam kampung yang terinfeksi cacing cestoda. Sampel usus halus ayam kampung diambil dari dua pasar yang berbeda, yaitu pasar Pluit di Jakarta Utara dan pasar Kebayoran Lama di Jakarta Selatan. Hasil: Hasil pengamatan histopatologis pada preparat usus ditemukan deskuamasi epitel vili dan proliferasi sel-sel kripta yang diakibatkan oleh infeksi cacing cestoda. Sel goblet yang mengalami hipertrofi paling banyak ditemukan pada sampel preparat yang diambil dari pasar Kebayoran Lama, Jakarta Selatan (p>0,05). Simpulan: Dapat disimpulkan bahwa deskuamasi vili dan jumlah hipertrofi sel goblet terjadi akibat infeksi cacing cestoda pada mukosa usus halus ayam kampung.
In vitro and in vivo effects of curcumin on oral cancer: a systematic review Ridho, Fiki Muhammad; Syachputra, Andika Julyanto; Fahrudin, Panggih; Nurhuda, Andang; Nurliana, Nurliana; Latuamury, Nadhia S
Current Biomedicine Vol. 2 No. 2 (2024): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.2.2.101-115

Abstract

Background: Current therapy for oral cancer (OC) patients, including surgery, radiotherapy, and chemotherapy, still have many shortcomings. Therefore, the discovery of natural products to prevent and treat cancer is receiving increasing attention, one of which is curcumin. Curcumin (diferuloylmethane) is a polyphenolic compound found in turmeric (Curcuma longa) and has been widely used as a herbal medicine because of its effects on health, one of which is as an anticancer agent. Objective: This study aimed to systematically and comprehensively review and summarize the anticancer effects and mechanisms of action involved of curcumin on OC cells. Methods: A systematic review methodology was employed adhering to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines to review and summarize previous studies published in databases, including PubMed, ScienceDirect, and Google Scholar. The final results included 14 articles, both in vitro and in vivo studies. Results: Based on several preclinical studies regarding the effects of curcumin on OC cells, we highlight that curcumin has a strong potential in inhibiting OC cells through exerted effects such as immunomodulatory and anti-inflammatory effects, through inhibition of cell proliferation, invasion and migration, and angiogenesis, as well as through the induction of apoptosis and autophagy. Conclusion: The systematic review presented in this paper concludes that curcumin possesses the potential to inhibit the development of OC cells through several mechanisms of action related to immunomodulatory effects, anti-inflammatory effects, cell proliferation, invasion and migration, angiogenesis, apoptosis, and autophagy.

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