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Jurnal Laboratorium Khatulistiwa
Published by Poltekkes Kemenkes Pontianak
ISSN : 25979523     EISSN : 25979531     DOI : https://doi.org/10.30602/jlk
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology
The aim of this journal publication is to disseminate the conceptual thoughts or ideas and research results that have been achieved in the area of Medical Laboratory. Jurnal Laboratorium Khatulistiwa particularly focuses on the main problems in the development of the Medical Laboratory health areas as follows: Toxicology Immunoserology Bacteriology Clinical Chemistry Parasitologi Micology And other related disciplines.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Imunologi dan Mikrobiologi (Lain-Lain)
Articles 8 Documents
 1 
Search results for , issue "Vol 7, No 2 (2024): Mei 2024" : 8 Documents clear
Potensi Air Perasan Kunyit Putih, Kunyit Kuning Dan Kunyit Hitam Terhadap Bakteri Escherichia coli Penyebab Diare Sari, Emilda; Suwandi, Edy; Triana, Linda; Nurhidayattulloh, Ariffialdi
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1392

Abstract

Turmeric is a herbal plant that is widely used by the public (Curcuma domestica Val) which is proven to contain ingredients that can function as an antibacterial. The antibacterial properties of turmeric rhizomes are caused by its main chemical content, namely curcuminoids and essential oils. Other active substances in turmeric that can be used as antibacterials are flavonoids, alkaloids and tannins. This compound functions as an antibacterial.This research aims to explain the differences in the inhibitory power of White Turmeric, Yellow Turmeric and Black Turmeric juice against Escherichia coli bacteria. The research design used was quasi-experimental. The samples in this study were the juice of White Turmeric, Yellow Turmeric and Black Turmeric which was repeated 10 times for each treatment.Based on the research results, it is known that white turmeric rhizome juice has an average barrier potential of 33.7 mm against Escherichia coli bacteria. This is no different from the juice of Yellow Turmeric rhizomes which has an average barrier potential of 32.7 mm against Escherichia coli bacteria. Meanwhile, the juice from Black Turmeric has a smaller potential resistance compared to the juice from the rhizomes of White Turmeric and Yellow Turmeric, namely an average of 26.2 mm.
Identifikasi Bakteri Coliform pada Minuman Air Tahu dan Air Tebu yang Dijual di Wilayah Kota Pontianak Ramadan, Aditiya; Jordan, Aristo; Ardhani, Adha Riesta; Monalita, Ria; Munardi, Fhatia Natasya; Syahdilla, Ade; Nuswantoro, Ari; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1470

Abstract

Air tahu dan air tebu merupakan minuman manis dan menyegarakan yang cukup diminati oleh masyarakat Kota Pontianak terutama dari kalangan anak-anak, remaja, dan orang tua. Cemaran bakteri Coliform yang terdapat pada minuman air tahu dan air tebu yang dijual di Kota Pontianak dapat menyebabkan masalah serius pada kesehatan. Tujuan dari penelitian ini adalah untuk mengetahui adanya cemaran bakteri Coliform pada minuman air tahu dan air tebu yang dijual di pinggir jalan Kota Pontianak dengan menggunakan metode kultur. Desain penelitian ini adalah penelitian deskriptif dengan pengambilan sampel secara total sampling. Penelitian ini dilakukan dengan cara mengambil sebanyak 43 sampel air tahu dan 70 sampel air tebu yang dijual di pinggir jalan di Kota Pontianak. Hasil penelitian dari 43 sampel air tahu menunjukkan 17 sampel (39,5%) positif bakteri Coliform, 26 sampel lainnya (60,5%) negatif bakteri Coliform, dan hasil peneilitan dari 70 sampel air tebu menunjukkan 37 sampel (52,8%) positif bakteri Coliform, dan 33 sampel lainnya (47,2%) negatif Coliform.
Proteksi Biji Kopi Dalam Memperbaiki Morfologi Sperma Pada Tikus Yang Diinduksi Streptozotocin Maulana, Mochamad Rizal; Sofyanita, Eko Naning
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1421

Abstract

Pemberian induksi Streptozotocin (Stz) dapat menurunkan kualitas sperma salah satunya morfologi sel spermatozoa, kopi mempunyai kandungan antikosidan yang tinggi yaitu asam klorogenat yang merupakan antioksidan kuat, mampu bertindak sebagai antioksidan eksogen yang melawan ROS dalam sel. Tujuan dari penelitian ini untuk mengatahui pengaruh pemberian kopi pada tikus yang diinduksi Stz. Post Test Control Group Design dengan sampel 24 tikus jantan Rattus norvegicus umur 3 bulan, berat badan ± 250 gram, dibagi menjadi 4 kelompok secara random. Setelah 1 minggu aklimasi, pengobatan diberikan 1 kali sehari selama 14 hari. Hari ke 15 dilakukan pemerkksaan morfologi sperma.. Hasil penelitian morfologi spermatozoa (%) pada kelompok KN (50,46) kelompok KP (64,58) kelompok KK1 (59,50) dan kelompok KK2 (72,05) berbeda artinya, p< 0,05. Kesimpulan pada penelitian ini yaitu Pemberian kopi mampu memperbaiki morfologi spermatozoa pada tikus yang diinduksi Stz.
HUBUNGAN SEKS BEBAS DENGAN KEJADIAN SIFILIS DI WILAYAH KERJA PUSKEMAS KOM YOS SUDARSO KOTA PONTIANAK Dj, Herlinda Djohan; Sutriswanto, Sutriswanto; Nurhayati, Etiek; Ratika, Meila; Salim, Maulidiyah
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1347

Abstract

Syphilis, also known as "the lion king", is an STI (Sexually Transmitted Infection), a disease caused by infection with the Treponema Pallidum bacteria. It is chronic and chronic. This is a dangerous disease because it can attack all organs of the body. This research was conducted at the Kom Yos Sudarso Pontianak Community Health Center because the Community Health Center is a referral health center for syphilis examinations and the respondents were people who were caught by field officers who allowed free sex.               This study aims to find out who suffers from syphilis among respondents who engage in casual sex, as well as finding out the percentage of syphilis incidents in people who engage in casual sex and to find out the relationship between casual sex and the incidence of syphilis in the work area of the Kom Yos Sudarso Community Health Center, Pontianak City.               This research design uses cross sectional with random sampling technique. The samples used were 123 patient blood samples using the TP Rapid method.               Based on the results of the research conducted, casual sex and the incidence of syphilis were obtained. The results of analysis using the chi square test using Continuity Correctionb obtained a value of p = 0.021 < 0.05, so HA was accepted so that there was a significant relationship between casual sex and the incidence of syphilis in the work area of the Community Health Center. Yos Sudarso, Pontianak City.
EFEKTIFITAS KOMBUCHA TEH BUNGA ROSELLA DALAM MENGHAMBAT PERTUMBUHAN BAKTERI ESHERICHIA COLI DAN SHIGELLA DYSENTERIAE UNTUK MENCEGAH TERJADINYA DIARE Fatayati, Imma; Djohan, Herlinda; Roofiif, Sayyid Al
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1348

Abstract

One probiotic product is kombucha tea. Kombucha tea can be combined with rosella tea. Some of the bacteria that attack the digestive tract are Escherichia Coli and Shigella dysenteriae which can cause diarrhea. It has been suggested that probiotics can be used to prevent diarrhea. This research aims to determine whether there are probiotic benefits of Rosella flower kombucha fermented for 7 days and 14 days to prevent diarrhea. Review of the growth inhibitory power of Escherichia Coli and Shigella dysenteriae bacteria. This research is in the form of Quasi-experimental. The population of this study was rosella flower kombucha. The sample for this research was rosella flower kombucha with 7 days of fermentation and 14 days of fermentation. The number of repetitions for each treatment was 16 replications, with total replications was 62 replications. The results of this research showed that the inhibitory power of rosella flower kombucha against Shigella dysenteriae bacteria with an average fermentation time of 7 days was 11.31 mm (strong), for 14 days of fermentation the average was 24.72 mm (very strong). Meanwhile, the inhibitory power of rosella flower kombucha against E. Coli bacteria with a fermentation time of 7 days averaged 11.09 mm (strong), for 14-day fermentation, the average was 20.34 mm (very strong). This research concluded that the inhibitory power of rosella flower kombucha fermented for 7 days was in the strong category and 14 days in the very strong category for both the growth of Shigella dysentriae and `E. coli bacteria.
PERBEDAAN MODIFIKASI LARUTAN ZnS (Zinc sulfite) DENGAN NaCl (Natrium Cloride) 0.9 % METODE FLOTASI TERHADAP JUMLAH TELUR CACING Soil Transmitted Helminth (STH) Siregar, Saadah; Manurung, Arnita Elisabet; Rizky, Vincentia Ade; Krisdianilo, Vinsensius
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1405

Abstract

Worms is a disease caused by the entry of parasites in the form of worms into the human body due to the influence of environmental factors or food that is not kept clean. Factors that cause the risk of helminthiasis in humans include food that is infected with worm eggs, feet that are in direct contact with soil containing worm vectors because they do not wear footwear, the habit of defecating in random places, availability of clean water, hand washing, washing habits. plates, And nail hygiene. The purpose of this study was to determine the difference in the number of worm eggs to be found using ZnS 1% and NaCl 0.9% solution using the flotation method. The data obtained from faecal examination using the flotation method with ZnS 1% and NaCl 0.9%, namely Soil Transmitted Helminth (STH) worm eggs, then the number of eggs will be presented in table form and analyzed descriptively. Result With the average yield of worm eggs found in  a solution of ZnS  1% and NaCl 0.9% using the flotation method for the number of Soil Transmitted Helminth (STH) worm eggs. In ZnS 1% solution the average is 2.12 with 4 Ascaris lumbricoides eggs, Trichuris trichura 2 and 3 hookworms while in 0.9% NaCl solution the average is 1.31 with 5 Ascaris lumbricoides worm eggs, Trichuris trichura 1 and worms mine 1. The Manny Whitney statistical test showed results of Sig 0.003 (P<0.005), so it was concluded that there was a difference in the number of STH worm eggs found using ZnS 1% solution and 0.9% NaCl using the flotation method.
PENGARUH ANTIBAKTERI SEDIAAN SABUN WAJAH CAIR EKSTRAK ETANOL DAUN BELIMBING WULUH (Averrhoa bilimbi L) TERHADAP Staphylococcus aureus METODE DIFUSI Tumpuk, Sri; Suriansih, Suriansih; Nurhayati, Etiek
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1391

Abstract

Facial soap used to clean the face from exposure to dust, pollution, dirt, reduce sebum on the face and eradicate bacteria that can cause acne. Star fruit leaves are efficacious in healing several diseases, such as purulent skin inflammation, boils and medicine for gout. Phytochemical of star fruit leaves contain alkaloid, flavonoid, saponin, tannin and steroid compounds that can inhibit bacterial growth. This study aims to determine the effect of liquid facial soap containing of star fruit leaf extract (Averrhoa bilimbi L) on the growth of Staphylococcus aureus bacteria by diffusion method.This study used a Quasi Experiment research design. The population in this study were wuluh star fruit leaves. The sample used is star fruit leaf extract which is made into liquid facial soap preparations with concentrations of 5%, 10%, 15% and 20%. This antibacterial testing method uses the Kirby-Bauer diffusion method. The results of antibacterial research obtained the average diameter of the inhibition zone at 5% concentration of 10.60 mm, 10% concentration of 11.20 mm, 15% concentration of 12.10 mm, and 20% concentration of 13.23 mm. Based on the diameter of the inhibition zone formed at concentration of 5% is categorized as a moderate response and at concentration of 10%, 15% and 20% is categorized as a strong response.The results of simple linear regression statistical analysis obtained p value = 0.000 < α 0.05 which means Ha is accepted, which means that there is a significant effect on the preparation liquid facial soap ethanol extract of star fruit leaves (Averrhoa bilimbi L) against the growth of Staphylococcus aureus diffusion method.
Gambaran Jumlah Leukosit Pada Penderita Suspek So TB Paru di Rumah Sakit Patuh Patut Patju Reginata, Ni Luh Vira; Rohmi, Rohmi; Manu, Thomas Tandi; Khusuma, Ari
Jurnal Laboratorium Khatulistiwa Vol 7, No 2 (2024): Mei 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v7i2.1453

Abstract

Leukocyte examination is carried out to describe disease events or processes in the body, aspecially infectious diseases such as tuberculosis bacteria can attack immune system cells, including leukocytes. Bacteria can survive and reproduce in leukocytes. Pulmonary TB sufferers before treatment saw an increases in leukocyte levels, indicating the formation of many leukocytes to fight bacteria in the phatocytosis process. On of the laboratory tests used to support the diagnosis of tuberculosis is an examination of the leukocyte count. This study aimed to determine the description of the number of leukocytes in patients with Suspected SO Pulmonary TB at The Patuh Patut Patju Hospital Untuk. This study design is descriptive case study research. Sampling technique was carried out using accidental sampling technique and  30 respondents obtained. The results of this study showed that the average leukocyte levels of patients with suspected SO Pulmonary TB were 9,643/μl, with 13 people (1+), 12 people (2+), 5 people (3+). The highest average leukocyte count was found in the female gender, age group 56-65 years and on 5 months treatment

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