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Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions
JBBI is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original research papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees, and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, bioinformatics, as well as life sciences in general.
Arjuna Subject : Umum - Umum
Articles 17 Documents
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Search results for , issue "Vol. 11 No. 2 (2024)" : 17 Documents clear
STUDY OF ANTI-BACTERIAL AND ANTI-INFLAMMATORY PROPERTIES OF INDONESIA’S FLORA BIODIVERSITY AS DRUG CANDIDATES FOR DIABETIC ULCER THROUGH IN SILICO AND IN VITRO APPROACH Tiffanny, Cecilia; Regina, Aurelia; Chen, Davin Handreas; Rosyati, Margaretha Maria
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.2950

Abstract

This study is driven by the substantial increase in the number of diabetes mellitus patients, which is projected to triple by 2030 and the immense potential of Indonesia's extensive biodiversity. Diabetic ulcers represent one of the most common complications stemming from uncontrolled diabetes mellitus. Therefore, this research aims to determine the therapeutic potential of Indonesian biodiversity for diabetic ulcers, focusing on the anti-bacterial and anti-inflammatory properties of secondary metabolite derivatives, employing both in silico and in vitro approaches. Initially, the research employed an in silico approach, conducting molecular docking and molecular dynamics simulations on reference ligands obtained from the 6KVS receptor for Staphylococcus aureus bacteria and the 5KIR for the COX-2 protein. Molecular docking was performed for 18 secondary metabolite derivative test compounds using Autodock Vina, followed by molecular dynamics simulations. The results of the molecular dynamics simulations showed that flavanonols exhibited the highest stability among the test compounds, with an average root mean squared deviation of 0.139493 Å for anti-bacterial tests and 0.182499 Å for anti-inflammatory tests after 50 ns simulation. Based on the results of molecular dynamics simulations, in vitro anti-bacterial and anti-inflammatory testing was conducted using flavanonol compounds found in Ananas comosus plants. In the anti-bacterial test, the pineapple extract, at a 5% concentration, exhibited the largest inhibitory zone diameter, 3.53 mm. In the anti-inflammatory tests, the pineapple extract at an optimal concentration of 600 ppm showed the highest absorbance value for protein denaturation, measuring 0.11133 au.
MOLECULAR CHARACTERIZATION, PHYTOCHEMICALS SCREENING, AND MOLECULAR DOCKING OF CARDAMOM (Wurfbainia compacta), AND SAMBILOTO (Andrographis paniculata) AGAINST COVID-19 Kurniawati, Mufida Budi; Budiharjo, Anto; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3005

Abstract

Cardamom and Sambiloto are phytopharmaceutical plants that produce phytochemical compounds that have the potential to be used to increase immunity against COVID-19. because they contain carotenoids, phenols, anthocyanins, saponins, alkaloids and steroids. This research aims to obtain the molecular characteristics of Cardamom and Sambiloto plants from the Gunungpati area, Semarang by ITS primer, testing phenolic phytochemicals, tannins, flavonoids, saponins and alkaloids followed by molecular docking tests with the 6WX4 protein. SARS-CoV-2. Molecular characterization results show that Cardamom and Sambiloto are similar to Wurfbainia compacta MF802556.1 (100%) and Andrographis paniculate LC646073.1 (84.47%). The results of the phytochemical test screening showed that both plants contain flavonoids. Molecular docking tests were carried out with the compounds Quercetin, Avicularin, Naringenin, 5-hydroxy-7,8,2',5' tetramethoxyflavone, and Retinoic Acid. Retinoic Acid as a test ligand has the greatest potential in inhibiting the 6WX4 protein in the SARS-CoV-2 virus with a binding affinity value of -7.28 and RMSD 0.00.
TRANSDERMAL PATCH PILIS FROM TURMERIC RHIZOME AND BLACK SEEDS FOR TREATMENT BASED ON ETHNOPHARMACY Susanto, Nindita Clourisa Amaris; Sawitri, Arinda Handiyah; Muslimah, Intan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.4870

Abstract

Pilis, a traditional product containing various herbal ingredients, is currently less desir-able due to its impractical and unfashionable method of application. Pilis is used to treat dizziness, pain, eye fatigue, encephalitis in children, fever, and to aid recovery for mothers after childbirth. However, pilis also carries risks related to uncontrolled dosage and contamination, particularly from chemical or heavy metal pollutants, which may cause toxic reactions. To mitigate these risks, pilis should be used in recommended doses and produced under clean and safe conditions. This research aimed to provide the innovation of patches as an alternative treatment to increase attractiveness and control the active substances released and delivered for a long time. Transdermal patches are made from a combination of turmeric and black seed containing flavonoid active metabolite compounds, namely curcumin, carotenoids, quercetin, kaempferol, and apigenin. The combination has a synergistic effect that can increase total flavonoid levels and the therapeutic effect of pilis. The method used in this research was litera-ture review and data analysis. The results showed the effect of transdermal patch pilis from the combined extracts added to the formula in vitro and in vivo using animal tests. In conclusion, transdermal patch pilis has prospects as a topical treatment based on the combination of an active delivery system with a patch and metered dose system.
COMBINED TREATMENT OF LANDFILL LEACHATE USING COAGULATION-FLOCCULATION AND ANAEROBIC-AEROBIC BIOFILTER WITH BIOBALL Sugito, Sugito; Ratnawati, Rhenny; Laba, Vinsensius Fereri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5756

Abstract

Piles of waste in landfills can produce leachate that smells bad and contains organic and inorganic materials as well as some pathogenic bacteria. A biofilter is a simple method that can be used to degrade pollutant parameters dissolved in leachate. This research aims to determine the efficiency value of Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD), and Total Suspended Solid (TSS) concentrations in the leachate of Blandongan Landfill, Pasuruan City. The leachate treatment process uses the coagulation method as pretreatment with aluminum sulfate coagulants (Al2(SO4)3) and Ferric Chloride (FeCl3) followed by biofilter using bioball media. The variables in this research were the differences in the concentration of the coagulant used and the bioball media with a height of 15 cm. The results show that in bioball me-dia the value of reduction efficiency is better in reducing COD levels by 77.73%, BOD by 79.40%, and TSS by 81.48%. This research combines leachate treatment technol-ogy, where the coagulation-flocculation method becomes the pre-treatment of the leachate before continuing with an anaerobic-aerobic biofilter.
MICROBIAL CONTAMINATION AND BIOACTIVE COMPOUNDS OF JAMU BERAS KENCUR Gunardi, Wani Devita; Teiseran, Virginia Marsella; Timotius, Kris Herawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.5966

Abstract

Background: Jamu Beras Kencur (JBK) is recognized as an herbal medicine, containing two main components: the rhizome of Kaempferia galanga and rice. While previous research has identified bioactive compounds in galangal rhizomes, such as Ethyl p-Methoxycinnamate (EPMC), Ethyl-cinnamate, and Kaempferol, there are few reports on polar or aqueous compounds in JBK. LC-MS/MS and GC-MS enable comprehensive analysis of bioactive compounds, with LC-MS/MS detecting non-volatile, polar, and thermally sensitive compounds like flavonoids and glycosides, while GC-MS analyzes volatile and semi-volatile compounds, such as terpenoids, providing precise separation and identification. Therefore, this study were to know the amount and the growth of contaminant bacteria, yeast and mold; to determine the main bioactive compounds in JBK; and to determine the bioactive compound in aqueous and ethanolic extracts of rhizome that analysed with LC-MS/MS and GC-MS. Methode: JBK samples were sourced from local producers in West Jakarta, freshly prepared, and immediately analyzed for microbial contamination and bioactive compounds. Result: The analysis revealed microbial contamination in JBK, including Escherichia coli, Staphylococcus aureus, Coliform, yeast, and mold. Additionally, three novel flavonoid glycosides were identified: Chrysoeriol-4'-O-β-D-glucopyranoside, Patuletin-7-O-[6′′-(2-methylbutyryl)]-glucoside, and Acacetin-7-galactoside. Conclusion: Therefore, from the pharmacological perspective, JBK has the potentials as a healthy herbal drink. However, further preclinical and clinical studies are essential to validate its safety and efficacy for clinical use, which could pave the way for its integration into mainstream healthcare as a natural therapeutic option.
THE IN VITRO AND IN VIVO EFFECTS OF Persea Americana ETHANOL EXTRACT AS AN ANTIHYPERTENSIVE AND ANTIOXIDANT IN PREDNISONE-INDUCED RATS Elisa, Novi; Dian Advistasari, Yustisia; Sari, Wulan Kartika; Pratiwi, Mustika Endah; Tobi, Claudius Hendraman Boli; Murti, Bayu Tri; Putri, Athika Darumas
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.6583

Abstract

Hypertension impacts the quantity of free radicals within the body. This study aimed to investigate the effect of antihypertension based on free radical levels in the predni-sone-induced rats, focusing on evaluating specific anti-hypertensive fraction of avoca-do leaves. The study's methods conducting GC-MS, anti-hypertensive, and assessing malondialdehyde levels and catalase enzyme. Systolic blood pressure measurements indicated that the positive control (131 ± 3 mmHg) and ethyl acetate fraction (136 ± 4 mmHg) were statistically similar, significantly different from the negative control group. Diastolic blood pressure measurements showed a blood pressure decreasing in posi-tive control (105 ± 7) that similar statistically with ethyl acetate fraction (104 ± 6), and significantly difference with negative control group. Malondialdehyde levels were nota-bly elevated in both the positive control (37.79 ± 5.47) and ethyl acetate fraction (38.01 ± 5.47) compared to the negative control (-9.07 ± 10.10), indicating increased free rad-ical. Catalase activity demonstrated significant differences, with the positive control (44.06 ± 5.44) and ethyl acetate fraction (44.05 ± 5.45) showing similar levels, both substantially higher than the negative control (13.90 ± 21.50). In summary, this study indentificates several flavonoid compounds that promising anti-hypertension effect from ethyl acetate fraction of avocado leaves extract.
SCALE-UP OF PHENOLIC COMPOUNDS PRODUCTION FROM Styrax paralleloneurum LEAVES AND EXTRACT PURIFICATION USING AMBERLITE XAD-2 Martgrita, Merry Meryam; Sianipar, Imelda Febrika; Manurung, Adelina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.7431

Abstract

The current study aimed to determine the total phenolic compound content, antioxidant activity, and antibacterial activity of fermented Styrax leaf extract in 2000 mL volume. Fermentation by Aspergillus niger was carried out for 64 hours. After extraction, purifi-cation was conducted using Amberlite XAD-2 resin in ion exchange chromatography. The total phenolic content of the unpurified extract was 92.75 mg GAE.g-1, while the purified extract was 146.67 mg GAE.g-1. Antioxidant activity expressed in IC50 values was 31.71 ppm for the unpurified extract and 14.11 ppm for the purified extract. The antibacterial activity tested on Staphylococcus aureus and Pseudomonas aeruginosa showed an inhibition zone diameter of 31.43 mm and 28.2 mm, respectively for the unpurified extract, while for the purified extract 33.96 mm and 35.5 mm, respectively. It can be concluded that the purification of fermented Styrax leaf extract caused a stronger antioxidant activity and a very potent antibacterial activity.
UTILIZATION OF OIL PALM EMPTY FRUIT BUNCHES ENHANCED WITH MOLASSES FOR XYLITOL PRODUCTION Clarissa, Clarissa; Valerie, Levina; Hadi, Alvinsyah Ramdani Pradipta; Purnamadjaja, Andrew Medha; Valentino, Figo; Kembaren, Riahna
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.7739

Abstract

A minimal oil palm empty fruit bunch (OPEFB) usage encourages increased OPEFB utilization to prevent its loss of economic prospects. The xylose and arabinose constituents of OPEFB’s hemicellulose part can be utilized as raw materials for xylitol production. A high diabetes mellitus and periodontal disease prevalence in Indonesia makes xylitol a safe and helpful low-calorie sweetener alternative. This research explores the OPEFB molasses-supplemented media and compares Moniliella pollinis SP5 and other M. pollinis ethyl methanesulfonate (EMS) mutants in xylitol yields. It was found that M. pollinis SP5 accomplished 27% better performance in OPEFB+15%(v/v) molasses than in OPEFB from 0.48 g/g to 0.61 g/g. In addition, M4, M5, and M6 mutants from EMS mutagenesis generated more xylitol concentration in OPEFB + 15%(v/v) molasses compared to the SP5 strain. These findings provide in-sights into the potential of xylitol manufacture with OPEFB. For future research, it is recommended that xylitol production employing OPEFB be optimized.
PREVENTION OF GASTROPROTECTIVE ACTIVITY OF CHINESE BETEL EXTRACT (PEPEROMIA PELLUCIDA L.) ON THE HISTOPATHOLOGICAL FEATURES OF THE STOMACH IN A MOUSE MODEL OF GASTROENTERITIS Savitri, Lisa; Prasetyawan, Fendy; Saristiana, Yuneka; Juwita, Syntia Tanu; Amanda, Cornelia; Mebung, Konradus Klala
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8156

Abstract

Gastroenteritis is a common gastrointestinal disorder characterized by inflammation of the stomach and small intestine, often leading to symptoms such as diarrhea, vomit-ing, and abdominal pain. This study investigates the gastroprotective effects of Pepe-romia pellucida (Chinese betel) extract on histopathological features of the stomach in a mouse model of gastroenteritis. Male Swiss strain mice, aged 2-3 months, were treated with different doses of P. pellucida extract (100, 300, and 500 mg/kg BW) for 7 days after inducing gastroenteritis with Escherichia coli infection. Histopathological ex-amination showed that the extract significantly reduced cell degeneration, necrosis, and polymorphonuclear leukocyte infiltration in the stomach compared to the negative control group. The highest dose (500 mg/kg BW) exhibited the most prominent gastro-protective effect. These results suggest that P. pellucida possesses promising thera-peutic potential for gastroenteritis, possibly due to its bioactive compounds such as flavonoids, tannins, and saponins. Further studies are needed to explore the underly-ing mechanisms and optimize its therapeutic application.
UTILIZATION OF PINEAPPLE PROCESSING LIQUID WASTE IN BIODEGRADATION OF DISPOSABLE FACE MASK BY BACTERIA FROM LAMPUNG BAY Deviany, Deviany; Zhofiroh, Nawrah Meisyah Muthi'ah; Febrina, Yane Fita; Yusuf, Reggina Aulia; Achmad, Feerzet; Yuniarti, Reni; Istiadi, Khaerunissa Anbar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.8192

Abstract

The designation of COVID-19 as a global pandemic led to an increased use of single-use face masks, which result in waste that is difficult to degrade and has the potential to release microplastic fibers into the environment. This study aims to examine the im-pact of adding pineapple peel liquid waste (LCN) as a growth medium for the biodeg-radation of single-use face masks by bacterial isolates obtained from the waters of the Lampung Bay. The study investigates how LCN affects the efficiency of mask degra-dation by microorganisms, as well as how the ratio of LCN mixed with other growth media, such as Nutrient Broth (NB), influences the degradation process. The biodeg-radation process was carried out using a biostimulation technique, where bacterial iso-lates were incubated in media containing LCN at a 1:1 ratio. The degradation process lasted for 15 days, with the results being analyzed using gravimetry and Fourier-Transform Infrared Spectroscopy (FTIR). Gravimetric results showed a greater weight reduction in treated masks compared to the control masks. FTIR analysis also indicat-ed changes in the intensity of functional groups in the degraded layers of the masks, as well as the emergence of C≡C functional groups in the second and third layers. This study demonstrates that the addition of LCN can accelerate the biodegradation of sin-gle-use face masks, offering a new approach for managing mask waste.

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