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Awalul Fatiqin
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KAMPUS UPR TUNJUNG NYAHO Jln. Yos Sudarso Palangka Raya 73111, Kalimantan Tengah , Palangkaraya, Provinsi Kalimantan Tengah, 73111
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INDONESIA
Journal of Biotropical Research and Nature Technology
Published by Universitas Palangka Raya
ISSN : -     EISSN : 29640431     DOI : 10.52850/borneo
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Journal of Biotropical Research and Nature Technology is an open access, peer-reviewed journal dedicated to the publication of novel research concerned with the advancement of Biodiversity studies of Wetland tropical—and especially Indonesian—biodiversity. Original research articles are written in English, Bahasa and featuring well-designed studies with clearly analyzed and logically interpreted results are accepted, with a strong preference given to research that has the potential to make significant contributions to both the field of biology and society in general.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 44 Documents
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Uji Karakteristik Biokimia Kandungan Protein Total Ampas Organik Ecoenzyme nada wafiq hijriah, Nada wafiq; Siska Alicia Farma; Irma Lailani Putri; Resti Fevria
Journal of Biotropical Research and Nature Technology Vol. 2 No. 2 (2024): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v2i2.8448

Abstract

Organic waste can be utilized to become ecoenyme. Ecoenzyme is the result of organic waste baskets such as kitchen waste which are not valuable into environmentally friendly enzymes that are very useful. Ecoenzyme is a complex solution produced by a basket of fresh organic waste (fresh fruits and vegetables), brown sugar or molasses and water. Ecoenzymes are organic compounds. The liquid produced by heating ecoenzyme can be used as a disinfectant and hand sanitizer, in the health sector ecoenzyme can be used as an infection and allergy reliever in children and to heal wounds. Apart from the liquid which has many benefits, the dregs resulting from cooling ecoenzyme can also be used in agriculture as organic fertilizer. At this time the agricultural sector still uses a lot of inorganic fertilizers (chemical fertilizers), excessive application of inorganic fertilizers can cause physical, chemical and biological damage to the soil. Will have an impact on plant metabolism. The use of ecoenzyme waste can be an environmentally friendly fertilizer solution. The research method used was descriptive research with the aim of obtaining total protein content from ecoenzyme waste. The method used is the Warburgh Christian method. The result of measuring the total protein content of dry ecoenzyme dregs was 0.050 mg/ml. Protein contains amino acids that can be used for plant growth (structural function) and enzymes (metabolic function). This amino acid can increase the amount of chlorophyll in plants, increase photosynthetic activity, and promote root growth. Amino acids can also regulate stomata optimally by controlling plant transpiration and increasing the reduction of carbon dioxide which will be converted into carbohydrates.
Pengaruh Konsentrasi Pelarut Terhadap Kandungan Flavonoid Total Daun Binahong (Anredera cordifolia) Menggunakan Metode Microwave Assisted Extraction Yudhantara, Sandi Mahesa; Rohmawati, Lili
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8583

Abstract

Binahong (Anredera cordifolia) merupakan tumbuhan yang secara empiris dipercaya memiliki beragam khasiat. Salah satu kandungan senyawa dalam daun binahong adalah flavonoid. Penelitian ini bertujuan untuk mengetahui pengaruh variasi konsentrasi pelarut etanol pada proses ekstraksi berbantu microwave terhadap kadar flavonoid total ekstrak daun binahong. Variasi konsentrasi pelarut etanol yang digunakan adalah 60%, 70% dan 96%. Proses ekstraksi menggunakan metode microwave assisted extraction (MAE). Penetapan kadar flavonoid total menggunakan spektrofotometri uv-vis. Kadar flavonoid total ekstrak etanol daun binahong pada konsentrasi pelarut 60%, 70% dan 96% masing-masing sebesar 0,103%; 0,138% dan 0,061%. Hasil analisis menunjukkan adanya pengaruh variasi konsentrasi pelarut terhadap kadar flavonoid total ekstrak daun binahong.
Keanekaragaman Spesies Kupu-kupu (Lepidoptera) pada Habitat Ekowisata Kawasan Hutan Desa Tahawa Kabupaten Pulang Pisau Tuju, Fandi; Fatiqin, Awalul; Kadafi, Ahmad Muammar; Decenly; Dirgantara, Made; Ulaan, Magdalin; Nugroho, Yuli
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8976

Abstract

Butterflies are part of biodiversity that is important to be preserved because the presence of butterflies in an ecosystem, especially forests, is very important, both as part of the food chain, pollinators, and food for other animals. This study aims to analyze the species diversity and abundance of butterflies (Lepidoptera) in the habitat of Tahawa Village Forest Area, Pulang Pisau Regency. The research method used an exploratory survey method with a sweeping technique following a transect line and then calculated Shannon-Wiener Diversity Index (H'), Evenness Index (E), and Species Richness Index (R1). Butterflies found in the Tahawa Village Forest Area consisted of 6 families divided into 15 species with a total of 63 individuals. The most species found came from the Pieridae family and the least from the Geometridae family. The Diversity Index (H') obtained a value of 2.38, classified as moderate diversity. The Evenness Index (E) is in the high category with 0.88, while the Richness Index (R1) is at 3.38 which is still classified as low.
Transpirasi Tiga Spesies Dominan dalam Konservasi Air di Daerah Tangkapan Air (DTA) Wonosadi Kabupaten Gunungkidul Fitriah, Umi Novita; Susanto, Suwarno Hadi; Aziz, Fadhila
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8990

Abstract

Abstrak Cuaca ekstrem di Indonesia menyebabkan terjadinya fenomena krisis air di beberapa daerah, salah satunya Gunungkidul. Daerah Tangkapan Air (DTA) Wonosadi Kabupaten Gunungkidul merupakan kawasan DTA yang luas dan mampu menyimpan air. Konservasi DTA perlu dilakukan untuk menjamin ketersediaan air. Keberadaan vegetasi tumbuhan yang ada di DTA memberi pengaruh besar terhadap ketersediaan air dan nutrien di tanah, karena vegetasi memegang peran penting dalam siklus hidrologi. Penelitian ini dilakukan untuk mengetahui peranan ekofisiologis dalam mengkonservasi air dalam proses transpirasi. Penelitian dilaksanakan selama lima bulan di DTA Wonosadi Kabupaten Gunungkidul dan Laboratorium Ekologi Fakultas Biologi Universitas Gadjah Mada. Sampel tumbuhan diperoleh melalui analisis vegetasi berdasarkan tiga jenis kerapatan vegetasi (tinggi, sedang, dan rendah). Pengukuran laju transpirasi dilakukan pada tiga spesies dominan di DTA Wonosadi dengan menggunakan kertas Kobalt klorida. Tiga spesies dominan yang diperoleh yaitu yaitu Swietenia macrophylla King., Tectona grandis L., dan Acacia auriculiformis A.Cunn. Ex Benth. Hasil penelitian menunjukkan bahwa Tectona grandis L., merupakan spesies yang paling berpengaruh besar terhadap ketersedian air dengan laju transpirasi 181.00 detik. Tingkat transpirasi tumbuhan juga dipengaruhi oleh faktor abiotik yaitu pH tanah, suhu tanah, suhu udara, kelembapan tanah, kelembapan udara dan intensitas cahaya. Hasil analisis faktor lingkungan menunjukkan bahwa masing-masing kelas kerapatan vegetasi menunjukkan perbedaan hasil di tiap parameter lingkungan. Area kerapatan tinggi memiliki suhu udara dan kelembapan yang tinggi dibanding kelas lainnya. Hal ini menunjukkan bahwa semakin rapat vegetasi maka semakin tinggi kelembapan di area tersebut. Abstract Extreme weather in Indonesia has caused water crisis in several regions, including Gunungkidul. Wonosadi Catchment Area (DTA) of Gunungkidul Regency is a large catchment area that capabled of storing water. Conservation of the catchment area are needed to ensure the availability of water. The existence of plant vegetation in the catchment area has a major influence on the availability of water and nutrients in the soil, because vegetation plays an important role in the hydrological cycle. This study was conducted to determine the role of ecophysiology in conserving water in the transpiration process. The study was conducted for five months at Wonosadi DTA, Gunungkidul Regency and Ecology Laboratory, Faculty of Biology, Gadjah Mada University. Plant samples were obtained through vegetation analysis based on three types of vegetation density (high, medium, and low). Transpiration rate measurements were conducted on the three dominant species in Wonosadi DTA using Cobalt chloride paper. The three dominant species obtained were Swietenia macrophylla King., Tectona grandis L., and Acacia auriculiformis A.Cunn. Ex Benth. The results showed that Tectona grandis L. has the greatest effect on water availability with a transpiration rate of 181.00 seconds. Plant transpiration rate is also influenced by abiotic factors, namely soil pH, soil temperature, air temperature, soil moisture, air humidity, and light intensity. Analysis of environmental factors showed that each vegetation density class shows different results in each environmental parameter. High-density areas have higher air temperature and humidity than other classes. It showed that the denser of vegetation, the higher humidity in the area.
Identifikasi Parasit Pada Saluran Gastrointestinal Kecoa Mirna, Mirnawati Dewi; Indri Aprilia; Andara, Aldo Jean; Supryatno, Adi
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8991

Abstract

Kecoa memiliki kemampuan secara mekanis menularkan parasit dan mikroorganisme penyebab penyakit ke manusia sehingga menimbulkan ancaman kesehatan masyarakat. Tujuan penelitian ini yaitu mengkaji jenis parasit yang terdapat pada saluran pencernaan kecoa. Penelitian ini merupakan penelitian observasional deskriptif dengan pendekatan cross sectional dan sampel diambil secara excidental sampling. Kecoa dikoleksi dari lokasi yang berbeda di sekitar pemukiman masyarakat kemudian dilakukan identifikasi. Kecoa tersebut jenis Periplaneta americana dan Blatta orientalis. Selanjutnya dilakukan pembedahan pada kecoa. Pembedahan dilakukan untuk mengeluarkan saluran pencernaan dari esophagus sampai rectum. Jenis parasit yang di temukan yaitu Hammerschmidtiella diesingi. Hasil penelitian ini mengungkapkan bahwa kecoa mampu menularkan parasit tersebut ke bahan makanan yang dilewati oleh kecoa jika kecoa tersebut bermigrasi ke rumah-rumah masyarakat. Penelitian ini menemukan nematoda Hammerschmidtiella diesingi yang diisolasi dari P. americana untuk pertama kalinya di Palangka Raya
Phytochemical Screening n-hexane Extract of Sponge Xestospongia testudinaria from Spermonde Archipelago Kalalinggi, Septaria Yolan; Soekamto, Nunuk Hariani; Firdaus; Appa, Felycitae Ekalaya; Irmawan, Muhammad
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8992

Abstract

One of the islands in Indonesia that is inhabited by sponges of various types is the Spermonde Archipelago, which is located in the southern part of the Makassar Strait. Sponge X. testudinaria is one of the sponges that dominates the Spermonde Islands. The difference in environmental conditions where the sponge lives causes the sponge X. testudinaria to have a different adaptation mechanism in producing secondary metabolites as self defense. Research on the of compounds from n-hexane extract sponge X. testudinaria has been carried out using phytochemical tests. Extraction begins with maceration using n-hexane solvent then the solvent is moved using an evaporator. Identification of extract functional groups using IR spectra. The results of the phytochemical screening indicated the presence of alkaloids and steroids which were supported by IR spectrum data indicating the presence of aliphatic NH, C=O and CH groups.
Quantitative Traits of Melodi Gama-3 Melon (Cucumis Melo L.), A New Superior Cultivar, on Rainy Season and Altitude Variation Hadi, Rizko; Sidiq, Yasir; Maryanto, Sigit Dwi; Daryono, Budi S
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8998

Abstract

Melodi Gama 3 is a superior melon cultivar candidate which was developed presently. Purposes of this study were to determine quantitative traits of the Melodi Gama 3, as well as to compare it to commercial melon cultivars, based on trial on rainy season and altitude variation. Materials used were Melodi Gama-3 seeds originated from Laboratory of Genetics, Faculty of Biology, Universitas Gadjah Mada, and commercial cultivars seeds, i.e., Action 434, Glamour Sakata, MAI, and LADIKA, respectively. The trial had been conducted at three variations of altitude i.e., mediumland, lowland, and beach shore, respectively, in period of rainy season. The quantitative traits, that were fruit weight and girth, flesh, and rind thickness, as well as vertical and horizontal diameter (longitudinal section), respectively, had been observed and measured at the Laboratory of Genetics, Faculty of Biology, Universitas Gadjah Mada. Data then analyzed with Honestly Significant Different test following balanced ANOVA, all using PKBT-STAT 2 software. Results showed that all the quantitative traits, except for flesh thickness, were influenced very significantly by interaction of cultivars x locations (P < 0.01). The Honestly Significant Difference test indicated that the Melodi Gama-3 had higher measurement of its quantitative traits (fruit weight: 2.68 kg; fruit girth: 55.45 cm; rind thicknesss: 0.93 – 1 cm; vertical diameter: 15.94 – 18.19 cm; horizontal diameter: 17.79 cm) than other cultivars. It was revealed that the Melodi Gama-3 was best planted at lowland altitude. Plant breeders should benefit from this study for the Melodi Gama-3 has great potency to be bred based on its traits comparison with commercial cultivars.
Identifikasi Spesies Candida Sp. Pada Urine Penderita Diabetes Mellitus: Literatur Riview Ngazizah, Febri Nur; Sobirin, Miftachul
Journal of Biotropical Research and Nature Technology Vol. 2 No. 1 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v2i1.9000

Abstract

Diabetes Mellitus merupakan penyakit yang diderita secar global. Penyakit ini ditandai dengan insulin tidak diproduksi atau insulin rendah sehingga menyebabkan glukosa yang meninggi ketingkat dimana jumlah glukosa yang difiltrasi melebihi kapasitas sel-sel tubulus melakukan reabsorpsi sehingga mengakibatkan glukosa muncul pada urine. Adanya glukosa pada urine ini menjadi habitat yang cocok untuk pertumbuhan jamur. Jamur yang umum ditemukan pada urine adalah Candida albicans. Selain C. albican juga ditemukan spesies candida yang lainnya dari genus candida. Penelitian ini bersifat deskriptif dengan data yang diperoleh dari studi literatur. Hasil studi litertur ini diketahui bahwa ada banyak kasus dibeberapa tempat terkait candida yang ditemukan pada sampel urine penderita DM yaitu sekitar 13-70% sampel positif mengandung candida. Spesies candida yang ditemukan pada sampel urine pasien yang menderita DM antara lain: C . albicans, C. glabrata, C. tropical, C. parapsilosis, C. kefir, C. lusitanae, C. guilhermondi, C. dubliniensis. C. kruseo dan C. auris. Cara identifikasi spesies candida yaitu: Pengamatan secara mikroskopis, kultur pada media CHROM agar, uji produksi chlamydospora, uji germ tube, uji gula-gula, test fermentasi karbohidrat, amplifikasi internal transcribed spacer regional (ITS), DNA squencing of internal transcriber space regional, PCR-RFLP, pemeriksaan  Magnetic Resonance Imaging (MRI).
Studi Awal Rhodobryum spp. di Sumatera dan Jawa (Koleksi Herbarium Bogoriense) Lestari, Ria Windi Lestari; Ginting, Nurmaini; Meriem, Selis
Journal of Biotropical Research and Nature Technology Vol. 2 No. 1 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v2i1.9016

Abstract

Documentation results of the collection bryophyte in Indonesia that exist in Bogoriense Herbarium is neglected. One of the collections is Rhodobryum tribe from Sumatera and Java. The Rhodobryum is easy to recognize by rosette leaf orientation looks like a rose flower. This genus was observed based on the bryophyte specimens collected in Bogoriense Herbarium. The results were obtained six Rhodobryum species, four of which are previously identification i.e. Rhodobryum russulum, R. giganteum, R.roseum, and R.ontariense. While two of the six unidentification yet i.e. Rhodobryum sp1, and Rhodobryum sp2 that have different characters with four species other.
Establishment of A Transient Expression Using PEG-Mediated Protoplast Transformation System in Black Rice Cempo Ireng Rahayu Opi Anggoro; Decenly; Yosua Hambit; Nanang Wahyu Prajaka
Journal of Biotropical Research and Nature Technology Vol. 2 No. 1 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v2i1.11019

Abstract

Indonesia black rice is a potential crop which consider to be develop as functional food because of high nutritional values. However, some agronomical traits, such as high culm and long harvesting time need to be improved for high productivity. Genetic engineering based on protoplast system is one of tools that can be used for improving black rice agronomical traits. The purpose of this study was to establish an efficient method for obtaining protoplasts, and to get information on whether the PEG-mediated transformation method can be carried out on black rice ‘Cempo Ireng’ using GFP transient expression as a marker. To get protoplast culture, we used callus and seedling as main explants. The results showed that 15th days seedling was the best explant source to get protoplast compare to callus. The combination of 1,5% macerozyme and 3% celulase was optimum to obtain the viable protoplasts. Transient expression of GFP can be done using PEG-mediated protoplast transformation in 30% concentration of PEG.

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