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Annales Bogorienses
Published by Badan Riset dan Inovasi Nasional
ISSN : 05178452     EISSN : 24077518     DOI : https://doi.org/10.55981/ann.bogor
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Annales Bogorienses aims to disseminate high-quality scientific research in the field of life sciences, with a strong emphasis on advancing knowledge and applications in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. The journal serves as a platform for researchers, academicians, and practitioners to share original findings, innovative methodologies, and critical reviews that contribute to scientific progress and sustainable development. The journal covers research in biotechnology, molecular biology, biochemistry, bioinformatics, and bioengineering. It publishes original research articles, reviews, and short communications, and is committed to rigorous peer review and open access for the widest possible dissemination of scientific knowledge.
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Articles 189 Documents
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EDITOR'S PREFACE Rachmawati, Syamsidah
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
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Massive In Vitro Propagation of Sandalwood Through Friable Embryogenic Callus Supatmi, Supatmi; Ardiyanti, Nurdiya; Rahman, Nurhamidar; Sudarmonowati, Enny
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
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Sandalwood (Santalum album), which belongs to Santalaceae family, is a commercially important tree in Indonesia due to its essential oil contents used for important essence of perfume in the perfumery industry. However, its population has significantly depleted since the planting materials of this tree using conventional methods are difficult to be provided. This study was conducted to mass propagate sandalwood using in vitro methods through friable embryogenic callus (FEC). The somatic embryos were formed using leaves in length of 1-3 and 4-7 mm cultured in MS medium containing 0.5 mg/l and 1 mg/l indole acetic acid (IAA), MS medium supplemented with 1 mg/l IAA and 0.2 mg/l kinetin and half concentration of MS medium supplemented with 1 mg/l Gibberellic acid (GA3). Primary somatic embryos (PSE) and secondary somatic embryos (SSE) then formed friable embryogenic callus when it repetitively transferred into MS medium supplemented with 1.7 mg/l BAP, 1 mg/l proline or 1.5 mg/l BAP and 1.2 mg/l kinetin every 3 weeks. The FEC shows its optimum maturation and regeneration in the MS medium supplemented with 1.5 mg/l BAP and 1.2 mg/l kinetin for 4-8 weeks. The acclimatization of sandalwood plantlets were perfectly conducted in the medium containing soil, sand and compos in ratio of 1:1:1 with the companion plant namely Murraya paniculata, (L) Jack which gave the best percentage of survival rate and the lowest percentage of fallen leaves. These findings may improve the massive propagation of sandalwood through FEC as well as a useful material for further genetic improvement of sandalwood by using FEC as material for genetic transformation.
Isolation and Characterization of Compounds from the Leaves of Pterocarpus indicus Willd and Their Antioxidant Activity Hartati, Sri; Angelina, Marissa; Meilawati, Lia; Dewijanti, Indah Dwiatmi
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
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The flavone glycoside was isolated from ethyl acetate fraction of ethanol extract of leaves Pterocarpus indicus Willd. The isolation was conducted by gravitation column chromatography and eluted successively with hexane, ethyl acetate and methanol by gradient, and purified by sephadex-LH20. The structure was elucidated base on spectroscopy data of NMR (1D and 2D), UV, LC-MS and FT-IR. Antioxidant was evaluated using 2,2-diphenyl-1-picrylhidrazyl (DPPH) radical scavenging. The isolation and identification led a stigmasterol as Compound 1 and a new flavonol-glycoside [(2R)-7-hydroxy-3-(3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yloxy)-2-(3,4,5-trihydroxy phe nyl)chroman-4-one] or ptevon-3-D- glycoside as Compound 2. Antioxidant activity of Compound 2 showed IC50 for 18.53 μmol and blank of quercetin was 7.94 μmol and Vitamin C was 40.25 μmol. These compounds and antioxidant activities are the first time reported from this plant.
Identification of a New Compound as α-Glucosidase Inhibitor from Aspergillus aculeatus Dewi, Rizna Triana; Suparman, Asep; Mulyani, Hanny; Darmawan, Akhmad; Lotulung, Puspa Dewi N.
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
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Terestrial fungi are of great importance as potential sources of pharmaceutical agent. Aspergillus aculeatus, a fungus isolated from soil sample collected in Indonesia, was cultured in liquid media to investigate a novel compound as inhibitor -glucosidase. The mycelium extract of A. aculeatus shows potential activity against Saccharomyces cereviseae -glucosidase and mild activity against mammalian -glucosidase with IC50 values of 9.57 μg/mL and 470.76 mg/mL, respectively. Enzyme assay-guided fractionation of this extract afforded rubrofusarin (1). Rubrofusarin, a linear naphtho--pyrone, is a natural pigment from Aspergillus sp. Interestingly, compound 1 shows potential inhibitory activity against mammalian -glucosidase (IC50 of 92.7 μg/mL), but no to S. cereviseae -glucosidase. The results suggest that A. aculeatus is a promising natural source as a lead compound in the discovery of antidiabetic drug.
Bioactivities Screening of Indonesian Marine Bacteria Isolated from Sponges Artanti, Nina; Maryani, Faiza; Mulyani, Hanny; Dewi, Rizna Triana; Saraswati, Vienna; Murniasih, Tutik
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
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Currently marine bacteria are considered as important source of natural products for drug discovery. The objective of this study is to conduct an in vitro bioactivities (antidiabetic, antioxidant and antibacterial) screening of 9 Indonesian marine bacteria isolated from sponges that belongs to the Research Center for Oceanography, Indonesian Institute of Sciences collections. The marine bacteria were cultured for 2 days in liquid medium containing yeast, peptone and sea salt under shaking condition and extracted with ethyl acetate. Antidiabetic was measured using inhibition of -glucosidase inhibitory activity method; antioxidant was measured using DPPH free radical scavenging activity method; antibacterial was tested using disc diffusion method. Screening results showed that at sample concentration of 200 μg/mL, there was significant -glucosidase inhibitory activity detected in the extracts of strain Sp 7.9 (84 % inhibition) and Sp 8.10 (75 % inhibition), however the antioxidant activities of these two strains were low only around 30 % inhibition, antioxidant activities of other strains were very low. Screening for antibacterial activities using 10 μL samples show that extract of strain Sp 8.5 was best for Staphylococcus aureus (14 mm inhibition); Sp 7.9, and Sp 8.5 for Bacillus subtilis (18 mm inhibition); Sp 8.10 for Escherichia coli (10 mm inhibition); Sp 8.9 and Sp 8.10 (10 mm inhibition) for Pseudomonas aeuriginosa. Based on these results marine bacteria strain Sp 7.9 and Sp 8.10 were selected to be used for further studies in the isolation of bioactive that has potential as antidiabetic and antibacterial. Results of molecular identification conducted by InaCC show that identity of both strains based on BLAST Homology using NCBI database were Bacillus thuringiensis.
Constitutive Expression of Candida antarctica Lipase B (CALB) in Pichia pastoris Using pGAPZα Vector Wahyuni, Febriana Dwi; Fuad, Asrul Muhamad; Suharsono, Suharsono
Annales Bogorienses Vol. 20 No. 1 (2016): Annales Bogorienses
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The synthetic gene of CalBsyn was previously constructed to encode Candida antarctica lipase B (CALB). Lipase of CalBsyn gene is slightly different from wild type CALB (CALB-wt) where it has three amino acids substitutions at different positions, i.e. V210I, A281E, and V221D, in order to improve its thermostability and catalytic efficiency. The CalBsyn gene was isolated from pJ912-CalBsyn vector by digestion using XhoI restriction enzyme. The 1136 bp fragment of CalBsyn gene was then ligated to pGAPZα expression vector and transformed into Escherichia coli TOP10F’ to obtain recombinant vector pGAPZα-CalBsyn. The result show that pGAPZα-CalBsyn recombinant vector was successfully transformed into E. coli TOP10F’ with transformation efficiency of 4.11x103 cfu/μg plasmid DNA. The pGAPZα-CalBsyn recombinant plasmid was successfully introduced into Pichia pastoris SMD1168H using electroporation method with transformation efficiency of 1.01x102 cfu/μg DNA. Recombinant protein expression was analyzed in several selected P. pastoris recombinant strains. Qualitative lipase activity assays results show that transformed P. pastoris-produced extracellular recombinant lipase (CALB) showing lipolytic activity; while results of quantitative lipase activity assays show that this Pichia-derived lipase achieved an activity of 6.35 Units/mL within 48 hours. SDS-PAGE analysis confirms the succesfull expression of CALB protein with molecular size was approximately 45 kDa.
Molecular Identification of Microalgae BTM 11 and its Lectin Isolation, Characterization, and Inhibition Activity Mustopa, Apon Zaenal; Isworo, Rhestu; Nurilmala, Mala; Susilaningsih, Dwi
Annales Bogorienses Vol. 20 No. 2 (2016): Annales Bogorienses
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BTM 11 is unknown species of microalgae which has active compounds that can inhibit viruses. Lectin is a carbohydrate-binding protein that is found in microalgae with antiviral and antibacterial activities. The purpose of this study was to perform identification, isolation, characterization, and assay of lectin inhibitory activity of microalgae BTM 11. The result shows that microalgae BTM 11 has homology with Cyanobacterium (99%) and Geitlerinema sp (98%). Lectin of microalgae BTM 11 has molecular weight of 17 kDa. Lectin protein activity of microalgae BTM 11 was able to inhibit the enzyme activity of RNA helicase hepatitis C by 57.90% and 27.55%. In addition, the protein was able to suppress the activity of Staphylococcus aureus ATCC 6538, E. coli EPEC K.1.1. and Salmonella typhii ATCC 25241. Activitiy of lectin was stable at 30 °C and unaffected by the action of the enzyme. These results indicate that lectin of microalgae BTM 11 could be an alternative to antiviral and antibacterial proteins.
Generation of mCherryBody: an Anti-Transferrin Receptor Antibody Variable Fragment Linked by The Fluorescent Protein mCherry Kusharyoto, Wien; Andriani, Dian; Handayani, Ira
Annales Bogorienses Vol. 20 No. 2 (2016): Annales Bogorienses
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A facile generation of a recombinant antibody fragment with intrinsic fluorescent properties of the monomeric mCherry fluorescent protein is described. The so-called mCherryBody was designed based on the structure model of the variable fragment of anti-transferrin receptor antibody LUCA31 and the X-ray crystallographic structure of the mCherry protein. mCherryBody was constructed to retain optimal spatial geometry between the C- and N-termini of the antibody light-chain (VL) and heavy-chain (VH) by mimicking the domains interface pairing in antibody Fab fragments and incorporation of the mCherry fluorescent protein as a bridging scaffold. The gene encoding the chimeric protein was cloned into the pJExpress414 expression vector, expressed and secreted into the periplasm of Escherichia coli NiCo21(DE3) for assembly and disulphide bond formation. Based on its amino acid sequence, mCherryBody was predicted to have a molecular weight of 51.46 kDa. The modular assembly used in the generation of mCherryBody may permit the interchange of binding sites and of fluorescent proteins to create robust panels of coloured antibody fragments. Thus, the mCherryBody platform facilitates rapid generation of colored single-chain variable fragment (scFv) chimeras that could be used for screening of antibodies against cell surface markers or receptors.
Characterization of Zygotic and Nucellar Embryo of Six Indonesian Mango Cultivars Using Molecular Markers Fatimah, Fatimah; Husni, Ali; Kosmiatin, Mia; Karsinah, Karsinah; Baroya, Mushlihatun
Annales Bogorienses Vol. 20 No. 2 (2016): Annales Bogorienses
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One of difficulties in mangoes hybridization is polyembryonic seeds. This phenomenon reduces the chance of recovering true hybrid seedlings. The identification of the zygotic embryo is difficult and the possible approach is by using molecular marker. The objective of this study was to evaluate the utilization of two different marker systems (dominant markers and SSR) to characterize the occurrence of zygotic or nucellar embryo in polyembryony mango cultivars Garifta Merah, Lalijiwo, Manalagi, Madu, Saigon Kuning, and Saigon Merah. The type of embryo was evaluated by comparing the exhibiting amplification patterns, if different from the mother plant considered as zygotic and identified as nucellar if they exhibited the same banding pattern as the mother plant. From both of this marker systems out of the 16 SSR and 16 evaluated dominant primers, nine primers of each systems amplified the largest number of allele and sharply defined band. Dendogram analysis showed that the evaluated dominant markers could distinguish the zygotic and nucellar embryo clearly compare to evaluated SSR primers. The number of zygotic embryos derived from SSR was 64% and dominant markers were 47%. Based on zygotic and nucellar size, number and position, indicating no relationship between the type of embryo and embryo size, number and the position.
EDITOR'S PREFACE Rachmawati, Syamsidah
Annales Bogorienses Vol. 20 No. 2 (2016): Annales Bogorienses
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