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Berita Biologi
Published by Badan Riset dan Inovasi Nasional
ISSN : 23378751     EISSN : 23378751     DOI : https://doi.org/10.55981/berita_biologi
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Berita Biologi is the journal features articles showcasing advancements in biological research and related sciences in Indonesia. Berita Biologi comprises original scientific papers presenting research findings, short communications, and reviews that are unpublished or not under consideration elsewhere. Covered topics are expected to introduce novel aspects or information.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 126 Documents
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VARIASI MORFOLOGI KEONG NERITIDAE DI PANTAI BADUR, MADURA Djoemharsjah, Intan Salsabila; Isnaningsih, Nur Rohmatin; Ambarwati , Reni
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.805

Abstract

Neritidae merupakan salah satu famili dalam Kelas Gastropoda dengan variasi morfologi spesies yang cukup tinggi. Hal ini terjadi karena anggota dari famili ini bersifat polimorfik, yang artinya satu spesies memiliki beragam warna dan pola cangkang. Penelitian ini bertujuan untuk mendeskripsikan variasi morfologi cangkang keong Neritidae di Pantai Badur, Madura, dan menganalisis karakter utama untuk identifikasi. Sampel diambil dari lokasi penelitian dan diidentifikasi berdasarkan 20 karakter morfologi. Data dianalisis menggunakan software NTSyspc 2.20 dan Ntedit 1.1b. Hasil analisis menunjukkan koefisien kesamaan tertinggi terdapat pada C dan E sebesar 0,95 karena merupakan spesies yang sama, yaitu Clithon oualaniense. Fenogram terbagi menjadi tiga kelompok besar, yaitu pada tingkat kesamaan 0,92 (Kelompok 1), 0,65 (Kelompok 2) dan 0,53 (Kelompok 3). Pemilihan karakter yang berbeda menentukan pengelompokan yang terbentuk, sehingga beberapa spesies dapat mengelompok dengan spesies lain dari genus yang berbeda.
POTENSI PREBIOTIC DARI EKSTRAK JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS) Setyawan, Ryan Haryo; Saskiawan, Iwan; Widhyastuti, Nunuk; Kasirah, Kasirah; Mulyadi, Mulyadi
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.806

Abstract

Nowadays, people are more aware of a healthy lifestyle and demand more functional food products. It leads to the raising of prebiotics and their health benefit, such as maintaining digestive tract health, decreasing heart disease and obesity risk, and improving the immune system. Since people need to diversify healthy food product, the pursuit of novel prebiotic ingredient which is potentially incorporated into functional food product needs to be done. One substance that has potency is β-glucan from white oyster mushroom or Pleurotus ostreatus. Accordingly, the aim of this study is to observe the prebiotic potency of white oyster mushroom extract. The study started by extracting β-glucan from white oyster mushroom powder by hot water extraction and subsequently proceeded into alkaline extraction. After that, β-glucan content of extracts and residue were measured by Megazyme® β-glucan assay kit and supplemented in glucose-free growth media to see whether it can be utilized by probiotic Lactobacillus plantarum Dad-13 and pathogen Escherichia coli InaCC B-4. After prebiotic index, prebiotic activity score, pH, and titratable acidity of each extract were compared to FOS and inulin, it showed that β-glucan from water extract of P. ostreatus has the potency to become a novel prebiotic substance. It has 37.15+1.27 g/100g β-glucan content, 1.42+0.05 prebiotic index, and 0.91+0.01 prebiotic activity score. It could be utilized by probiotic to produce organic acid, such as lactic acid as well.
MITIGASI CEKAMAN KADMIUM (Cd) PADA TANAMAN PADI (Oryza sativa L.): PENDEKATAN FISIOLOGI DAN MOLEKULER Meriem, Selis
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.807

Abstract

Kontaminasi kadmium (Cd) pada lahan pertanian dan budidaya padi sangatlah mempengaruhi produksi tanaman dan juga kesehatan manusia. Akumulasi Cd dalam jaringan padi menimbulkan peningkatan spesies oksigen reaktif yang menyebabkan kerusakan aktivitas metabolisme sehingga menghambat pertumbuhan tanaman. Jika keberadaan Cd yang tinggi tidak dapat direduksi selama fase pertumbuhan padi, penyerapan dan translokasi Cd ke bagian tajuk akan menyebabkan akumulasi Cd yang tinggi dalam bulir padi. Review ini bertujuan untuk mendeskripsikan pembahasan komprehensif mengenai respon fisiologis tanaman padi dari kultivar yang berbeda terhadap cekaman Cd, variasi praktik mitigasi untuk mengurangi toksisitas Cd, dan mekanisme toleransi melalui ekspresi gen yang ter-upregulasi. Lebih lanjut, strategi untuk mengurangi penyerapan Cd dan mengatasi stres oksidatif juga ditekankan dalam tulisan ini. Pemberian bahan amandemen dalam bentuk unsur kimia, biochar dan remediasi berbantu mikroba, fitohormon, maupun asam organik dapat diaplikasikan sebagai bentuk dari strategi remediasi Cd. Melalui hal tersebut, Cd dapat direduksi dalam bentuk kelat untuk dapat diimobilisasi, distabilkan, diekstraksi di permukaan akar, atau disimpan dalam sel akar. Selain itu, studi ini juga membahas beberapa overekspresi gen yang responsif terhadap Cd pada kultivar padi yang toleran. Upaya dalam meningkatkan ketahanan tanaman padi yang mampu membatasi serapan Cd dan mengurangi akumulasi Cd dalam bulir padi sangat penting mendapat perhatian serius demi menjaga keamanan pangan dan kesehatan manusia.
ANALISIS SPASIAL DAN DETEKSI TRANSMISI TRANSOVARIAL VIRUS DENGUE PADA NYAMUK AEDES AEGYPTI DESA CIKUYA DAN PAREREJA DI WILAYAH KERJA PUSKESMAS BANJARHARJO, KECAMATAN BANJARHARJO, KABUPATEN BREBES, PADA TAHUN 2020 Amin, Siti Asiyah Fitria; Mulyaningsih, Budi; Umniyati, Sitti Rahmah
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.808

Abstract

Indonesia termasuk negara tropis sekaligus negara endemis tinggi penyakit Demam Berdarah Dengue. Nyamuk Ae. aegypti merupakan salah satu vektor virus dengue. Penularan virus dengue dapat terjadi dimulai dari fase nyamuk masih dalam bentuk telur yang dinamakan penularan secara transmisi transovarial. Kepadatan penduduk yang tinggi dapat membuat penyebaran penyakit Demam Berdarah Dengue (DBD) lebih intensif karena jarak rumah yang berdekatan memudahkan nyamuk menyebarkan virus dengue. Penelitian ini dilakukan di Desa Parereja dan Cikuya yang merupakan daerah endemis tinggi DBD. Pengambilan sampel dilakukan di wilayah yang padat dan jarang penduduknya. Penelitian dilakukan secara observasional deskriptif menggunakan desain cross sectional untuk menganalisis pola sebaran kasus DBD berdasarkan analisis Average Nearest Neighbor (ANN). Pendeteksian transmisi transovarial virus dengue dilakukan dengan metode imunositokimia dan real time PCR. Hasil penelitian menunjukkan pola sebaran kasus DBD adalah berkerumun terutama di daerah padat penduduk dengan jarak rata-rata antar kasus adalah 100,616 meter. Angka Indeks Transmisi Transovarial (ITT) di Desa Parereja adalah 13,3% yang diperoleh dari metode deteksi imunositokimia dan nilai Cq 33,88 < NTC (Not Template Control) 35,74 diperoleh dari metode real time PCR. Sedangkan di Desa Cikuya nilai ITT 0% dan Cq 38,78 > NTC 35,74. Sebagai kesimpulan pola sebaran kasus DBD berkerumun terutama dijumpai di daerah yang padat penduduknya dan transmisi transovarial virus berpotensi terjadi.
PURIFIKASI FRAKSI PROTEIN IMUNOGENIK 47 kDa DARI KELENJAR SALIVA Aedes albopictus SEBAGAI TARGET PENGEMBANGAN VAKSIN DENGUE BERBASIS VEKTOR Wathon, Syubbanul; Rahmawati, Itsna; Oktarianti, Rike; Lelono, Asmoro; Senjarini, Kartika
Berita Biologi Vol 22 No 1 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.810

Abstract

Kapasitas vektorial Ae. albopictus telah diketahui sebagai vektor potensial virus dengue yang mengakibatkan DBD. Transmisi virus dengue terjadi ketika Ae. albopictus melakukan blood feeding ke manusia yang difasilitasi oleh aktivitas biologis protein pada kelenjar saliva vektor. Penelitian kami sebelumnya menunjukkan fraksi protein 47 kDa dari kelenjar saliva Ae. albopictus bersifat imunogenik. Protein 47 kDa merupakan serpins family yang diduga sebagai antikoagulan dan berperan sebagai protease inhibitor sehingga mempermudah transmisi virus dengue. Peran protein tersebut mengindikasikan adanya potensi dalam pengembangan vaksin penghambat transmisi patogen melalui vaksinasi melawan protein tersebut sehingga dapat menghambat transmisi dengue. Studi potensi protein tersebut memerlukan ekstrak murni, maka purifikasi protein target merupakan langkah yang sangat penting. Penelitian ini dimulai dengan landing collection dan rearing Ae. albopictus, isolasi dan ekstraksi protein kelenjar saliva, isolasi fraksi protein 47 kDa melalui SDS-PAGE, purifikasi protein, analisis dot blot dan western blot. Analisis SDS-PAGE menunjukkan pita tunggal protein target 47 kDa dari kelenjar saliva Ae. albopictus yang berhasil dipurifikasi menggunakan Electroeluter. Analisis dot blot menunjukkan protein 47 kDa terdeteksi bersifat imunogenik dan dikonfirmasi melalui analisis western blot bahwa protein target hasil purifikasi memiliki berat molekul 47 kDa.
DINAMIKA PERTUMBUHAN POHON DI HUTAN RESTORASI BODOGOL TAMAN NASIONAL GUNUNG GEDE PANGRANGO, JAWA BARAT Sadili, Asep; Salamah, Andi; Mirmanto, Edi; Kartawinata, Kuswata
Berita Biologi Vol 22 No 2 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.812

Abstract

The Restored Forest (RF) in Bodogol, Gunung Gede Pangrango National Park (GGPNP) is located in the degraded lowland forest area. The Bodogol RF constituted the area of the ecosystem restoration program, where the initial tree planting was carried out by Mrs. Megawati Soekarnoputri the 5th President of the Republic of Indonesia. In that restored forest area, a study was conducted by establishing one hektare plots. We recorded 26 tree species and 16 families, including 14 tree species that were used in the restoration, four cultivated species, and eight species that naturally enter the site as new arrival (recruitment). The total basal area was 7.47 m2/Ha and tree density was 256 trees/Ha. The mean diameter growth was 1.78 cm/year, and the highest diameter growth was Hibiscus tilliaceus (2.42 cm/year). The mean total height growth was 0.52 m/year, and the highest was Paraserianthes falcataria (0.85 m/year). The density of Toona sureni was very high (61 trees/Ha) and in the future it ay dominate the forest will, therefore other tree species native to Mt. Gede-Pangrango should be planted to enrich the present restored forest so as to encourage the development of mixed lowland or montane forests similar to the original climax forests with high species diversity. Structurally, about 50 % of the RF has been achieved, but species composition still needs further enrichment either by active intervention of planting native TNGGP species if deemed necessary or by allowing succession to take place naturally, although it will take a very long time.
PENGELOLAAN KOLEKSI MUSEUM ZOOLOGICUM BOGORIENSE (MZB) TAHUN 2022 Mujiono, Nova; Alfiah, Alfiah; Apandi, Apandi; Darmawan, Darmawan; Fatimah, Fatimah; Wikanta, Hadi; Haerul, Haerul; Kurnianingsih, Kurnianingsih; Wahyudin, Mohamad; Mulyadi, Mulyadi; Supriatna, Nanang; Santoso, Pramono Hery; Prihandini, Riena; Rachmatiyah, Rina; Sarino, Sarino; Sauri, Sopian; Suparno, Suparno; Nurhaman, Ujang; Sofyani, Umar; Trilaksono, Wahyu; Priyatna, Yayat
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.892

Abstract

Since the integration of LIPI into BRIN in 2021, the Museum Zoologicum Bogoriense (MZB), which is managed by the coordinator of the zoological collection assisted by 20 collection technician staffs, has been transferred to the Directorate of Scientific Collections Management. The process of integration and management transfer does not change the main duties and functions of MZB as the national depository for zoological specimen collections. In addition to managing collections, researchers frequently ask MZB staff for assistance during field research and teaching collection management courses to students, teaching staff, and regional museum directors. The management of the MZB collection is described in a scientific publication for the first time. We hope that this documentation will continue so that museum management science can advance and assist the scientific community, particularly in Indonesia.
ANALISIS PRODUKSI DAN DISTRIBUSI PEMBENIHAN IKAN KOI (Cyprinus carpio) BERDASARKAN SEBARAN KUALITAS SELEKSI DI OMAH KOI FARM INDONESIA Fauzan, Agung Lutfi; Budiardi, Tatag; Effendi, Irzal; Diatin, Iis; Hadiroseyani, Yani; Dewi, Nina Nurmalia
Berita Biologi Vol 23 No 1 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.905

Abstract

Koi fish (Cyprinus carpio) is a freshwater ornamental fish commodity that has important economic potential, both nationally and internationally. Breeding is an activity in cultivation to produce seeds which are very decisive at the next stage of cultivation activities. The purpose of this study was to evaluate the technique and distribution of hatchery based on the distribution of quality selection of koi fish at Omah Koi Farm Indonesia. The test parameters measured were fecundity, fertilization rate (FR), hatching rate (HR), seed survival, seed quality, and water quality. The hatchery process includes pond preparation, parent selection, spawning, hatching eggs, rearing larvae, harvesting larvae, stocking larvae, feeding, feeding seeds, harvesting seeds, selecting seeds, and managing water quality. The results of the Kohaku and Showa fecundity calculations were 30,000 and 35,000 items respectively. The FR value obtained is 90% and HR is 83%. The average survival of koi seeds aged 45 days was 81.67% with an average of 150 high quality (HQ) seeds, 450 grade A seeds, and 450 grade B seeds. The temperature range in the larval rearing ponds was 25-27 0C, the water pH ranged from 7.9-8.5, DO ranged from 5.0-6.0, and ammonia was 0.01.
ANALISIS GEN KOMPARATIF KARSINOMA SEL SKUAMOSA PARU-PARU ANTARA INDIVIDU MEROKOK DAN TIDAK MEROKOK [Comparative Gene Analysis of Squamous Cell Lung Carcinoma Between Smoking and Non-smoking Individuals] Shemuel, Josia; Angelique, Priscilla; Josephine, Evalina; Ryan Fugaha, Daniel; Gabriela, Vania; Alhussain, Shaheer; Parikesit, Arli Aditya
Berita Biologi Vol 22 No 3 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.908

Abstract

Squamous cell lung carcinoma (SCC) is a form of non-small cell lung cancer that commonly arises in the primary airway. The development of SCC is closely linked to changes in squamous cells that line the airways, primarily caused by exposure to tobacco smoke. To gain insights into SCC, bioinformatics techniques have been employed to detect biomarkers and analyze gene expression patterns, utilizing data from the Cancer Genome Atlas (TCGA) database, which was preprocessed for analysis. By employing DESeq2, a differential gene expression analysis method, identified genes showed significant variations in expression between smoking and non-smoking groups among the 11,530 genes examined. Notably, five genes, namely CT45A1, GCGR, TPTE, ABCC2, and PI16, were found to play a significant role in tumor development and were susceptible to under- or over-expression due to smoking. The majority of these genes were found to be underexpressed rather than overexpressed. These identified genes hold potential as biomarkers for tumor development and exhibit a strong correlation between smoking history and the development of SCC. However, a limitation encountered during this analysis was the unavailability of data from normal non-tumor patients, which could have facilitated a more comprehensive analysis of differential gene expression. Furthermore, this research gives a deeper implementation regarding the molecular mechanisms and genomics underlying SCC development, identifies differentially expressed genes associated with SCC and smoking history, and highlights potential biomarkers that warrant further investigation.
ANALISIS EKSPRESI RELATIF DAN PENYEJAJARAN GEN Bmr12 PADA AKSESI SORGUM (Sorghum bicolor L. Moench) Windiastri, Vincentia Esti; Sintho Wahyuning Ardie; Miyamoto, Takuji; Umezawa, Toshiaki; Satya Nugroho
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.952

Abstract

Gen Bmr12 (SbCOMT) diperkirakan mempunyai fungsi dalam penentuan kandungan total lignin pada sorgum karena mutasi gen Bmr12 menyebabkan kandungan total lignin tanaman mutan tereduksi. Pada penelitian sebelumnya telah dikelompokkan aksesi-aksesi sorgum di Indonesia berdasarkan kandungan lignin tinggi dan lignin rendah (Buleleng Empok, JP dan Pahat). Untuk mengetahui pola ekspresi gen Bmr12 pada kedua kelompok sorgum yang berbeda, maka dilakukan analisis level ekspresi relatif dari sorgum berlignin tinggi yang diwakili oleh aksesi KS, 4183, dan SMM; dan sorgum berlignin rendah yang diwakili oleh aksesi Buleleng Empok, JP dan Pahat. Sebagai data pendukung, dilakukan pula perunutan gen Bmr12 yang diisolasi dari aksesi KS, 4183, Buleleng Empok dan Pahat. Hasil analisis tersebut memperlihatkan adanya perbedaan pola ekspresi gen Bmr12. Level tertinggi ada pada aksesi 4183A, dan yang terendah adalah aksesi Pahat. Keempat aksesi lainnya mempunyai pola ekspresi yang hamper sama. Perunutan dan penyejajaran gen Bmr12 memperlihatkan adanya substitusi basa nitrogen yang mengarah ke substitusi asam amino pada aksesi KS. Namun substitusi tersebut tidak mengganggu fungsi gen Bmr12 dalam biosintesis lignin.

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