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INDONESIA
Jurnal Penelitian Saintek
ISSN : 14123991     EISSN : 25287036     DOI : 10.21831
Core Subject : Science,
Arjuna Subject : -
Articles 286 Documents
Antioxidant and antibacterial activities of alkaloids from endophytic bacterium Pseudomonas hibiscicola Wk isolated from waru (Hibiscus tiliaceus) leaves Sarjono, Purbowatiningrum Ria; Laksmitasari, Nuraini Dwi; Asy'ari, Mukhammad; Ngadiwiyana, Ngadiwiyana; Ismiyarto, Ismiyarto; Fachriyah, Enny
Jurnal Penelitian Saintek Vol 30, No 1 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i1.77501

Abstract

This study aims to investigate the antioxidant and antibacterial activity from alkaloid extract. Alkaloid compounds were isolated from the endophytic bacteria Pseudomonas hibiscicola Wk, which was obtained from Hibiscus leaves. The alkaloid compounds were characterized using thin layer chromatography. The isolated alkaloid compounds were tested for total phenol content, and antioxidant activity. The phytochemical analysis of the alkaloid compounds from the endophytic bacteria Pseudomonas hibiscicola Wk showed a positive result for alkaloids, confirmed by an Rf value of 0.8125 which is similar to the Rf value of alkaloids from the periwinkle plant, namely vindoline (Rf = 0.85). Alkaloid compounds from the endophytic bacteria Pseudomonas hibiscicola Wk had the highest total phenol content of 2.390 mg gallic acid/gram sample at a concentration of 10.000 mg/L. These results indicate that the alkaloid compounds obtained contain phenol groups. The IC50 value obtained from the isolated alkaloid compound was 99.70 mg/L, demonstrating strong antioxidant capacity, and has the ability to inhibit the growth of E.coli and S.aureus bacterial.
Artificial Artificial habitat design (Dogania subplana) the area Turtle Learning Center (TLC) Universitas Bengkulu Adriataspen, Milzen; Karyadi, Bhakti; Parlindungan, Deni; Ruyani , Aceng; Nursa’adah, Euis
Jurnal Penelitian Saintek Vol 30, No 2 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i2.86213

Abstract

Malayan soft- shelled Turtle (Dogania subplana) is a species of freshwater softshell turtle that inhabits streams and swamps. This species is considered threatened with extinction and is listed in Appendix III of CITES and as Least Concern by the IUCN. This study aims to design an artificial habitat as a conservation effort and to identify the behavioral adaptations of Dogania subplana. The research method used an ecology-based design. The research subjects were five individuals of D. subplana, consisting of three females and two males. Data collection methods included field observation, literature review, and documentation. Research instruments involved environmental sensors to measure abiotic data. Data analysis was conducted both qualitatively and quantitatively. Qualitative data were analyzed descriptively through systematic and accurate reviews based on field findings and supported with photographic documentation. Quantitative data were analyzed using the average percentage of observed behaviors, presented in graphs, and supported by measured environmental abiotic data. The study results outline the stages of artificial habitat design for D. subplana, which include: location design, area division based on function, addition of supporting biota, fencing, and acclimatization. Observations revealed three behaviors feeding, basking, and aggression which were influenced by environmental temperatures from 26–31°C. D. subplana behaviors were observed only in aquatic areas with sandy and rocky substrates, indicating that such behaviors are strongly influenced by both terrestrial and aquatic environmental conditions. The study concludes that the artificial habitat at the Turtle Learning Center (TLC) of the University of Bengkulu, designed with three zones land, mud, and water effectively supports the natural adaptations and activities of D. subplana, as indicated by their feeding, basking, and aggressive behaviors.
The effect of salung leaf extract (Psychotria viridiflora) on blood glucose levels in mice Dara, Niki Dwi Dia; Ruyani, Aceng; Wardana, Rendy Wikrama; Mayub, Afrizal; Karyadi, Bhakti
Jurnal Penelitian Saintek Vol 30, No 2 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i2.87757

Abstract

This research is crucial in the context of the rising global prevalence of diabetes, a metabolic disorder that significantly affects public health. This study aims to determine the effect of salung leaf extract (Psychotria viridiflora) on blood glucose levels in hyperglycemic mice (Mus musculus). The study used 25 mice (Mus musculus) weighing 20-40 grams, divided into 5 treatment groups (P). Each group consisted of 5 mice, including a negative control, positive control, and three experimental groups with the administration of P. viridiflora extract at various doses (0.0112; 0.0225; and 0.0337 g/kgBW). Blood glucose measurements were conducted four times, on days 0, 6, 12, and 18, which included an initial glucose measurement, post-alloxan induction, and subsequent measurements after treatment. Among the three tested doses, the highest dose (0.0337 g/kg BW) proved to be the most effective in reducing blood sugar levels in alloxan-induced M. musculus, with effectiveness comparable to the standard drug Glibenclamide. Thus, the extract of P. viridiflora leaves shows strong potential as an alternative herbal antidiabetic therapy for M. musculus.
Synthesis of precipitated calcium carbonate using chicken bones as a raw material for paper Erlinda, Riska Melati; Rahma Putri Salsabila; Suprihatin
Jurnal Penelitian Saintek Vol 30, No 2 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i2.89242

Abstract

This research discusses the synthesis of precipitated calcium carbonate (PCC) standard for raw paper materials from chicken bones. In the paper industry, PCC is used as a coating and filler to enhance paper's smoothness, brightness, and opacity. The primary material used in this research is chicken bones from the frozen food industry waste, solving environmental problems. Chicken bones were dried in an oven at 100°C, then crushed and sieved to 100 mesh. X-ray Fluorescence (XRF) analysis shows that chicken bones contain calcium at 98.83%. The high content can make chicken bones a raw material for PCC. This research uses variables of Na₂CO₃ solution and precipitation time with a temperature of 30°C.  The target of this research to produce high quality Precipitated Calcium Carbonate (PCC) with a calcite crystal structure suitable for industrial applications particularly as raw material for the paper industry. Based on the analysis results, the best conditions were obtained at a Na₂CO₃ concentration of 2.5 M and a precipitation time of 40 minutes. The XRF and XRD test analyses showed that PCC was a polymorph calcite with a particle size of 2.14752 µm. This research proves that chicken bones can be an alternative raw material for PCC by ISO 3262-2:1998 parameters for industry standards
Sedimentary layers identification of Bribin underground river in Gunungkidul utilizing the theory of HVSR microtremor Melyyanti, Meli; Nur Azis, Khafidh; Nurhanafi, Kholis
Jurnal Penelitian Saintek Vol 30, No 2 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i2.89754

Abstract

This study aims to determine and analyze geological conditions based on the dominant frequency (fo) and amplification factor (Ao) values, determine sediment layers based on vs values, and interpret sediment thickness based on ground profiles of shear wave velocity using the ellipcity curve method in the Bribin Underground River area, Gunungkidul. This research method uses microtremors taken directly in the field with a seismic instrument that produces microtremor signal data. The results of the study produced dominant frequency (fo) values ​​ranging from 2.07 to 14.9 Hz where low frequency values ​​(<2.5 Hz) are evidenced by the presence of limestone, while amplification (Ao) values ​​range from 1.42 to 3.81 which are mostly located in the medium and high zones, with a thin surface layer of about 5 to 10 meters with sediment thickness values ​​on the north to middle side being quite thick, while the south side shows thin sediment thickness because it is located around the Underground River flow and is dominated by karst mountain areas. Thus the information obtained can be used to develop the potential of natural resources and related geological risks.
Identification of the causes of convective clouds in extreme rainfall events with hail based on dual-polar radar imagery Rafi, Rayhan; Kuncoro, Dwi; Arzhida, Bima; Jannah Indriyani, Noor; Warjono, Warjono
Jurnal Penelitian Saintek Vol 30, No 2 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i2.89950

Abstract

This study aims to analyze the atmospheric dynamics behind the formation of convective clouds that produce hail through a multi-instrumental approach. The methods used include streamline map analysis, synoptic data from the Yogyakarta Climatology Station, Himawari-9 HCAI satellite imagery, and dual-polar radar data from the Ahmad Yani Meteorological Station. The results reveal local wind convergence and significant air pressure drop, triggering rapid and intensive formation of Cumulonimbus clouds. Radar products, such as CMAX and HAILSZ, indicate high reflectivity and the presence of large hail particles within the clouds. Hail size is estimated to range from 10–20 mm with a probability of more than 80%. Rain validation shows the highest accumulation of 74 mm/day in Minggir District, Sleman. This study emphasizes the importance of integrating satellite, radar, and surface observation data for detecting extreme weather in tropical regions.