cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 9, No 3 (2023): December 2023" : 8 Documents clear
High Pre-treatment Neutrophil-to-Lymphocyte ratio (NLR) and Platelet-to-Lymphocyte ratio (PLR) Shows Lower Progressive-free Survival and Overall Survival in Tyrosine Kinase Inhibitor-treated Lung Adenocarcinoma Kusumawardhani, Erna; Haryati, Haryati; Arganita, Fidya Rahmadhany
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19403

Abstract

Background: The role of Neutrophil-to-Lymphocyte ratio (NLR) and Platelet-to-Lymphocyte ratio (PLR) as an easy and inexpensive prognostic examination modality has different results. While the combination of the two has never been done.Objective: This study investigated the association between NLR/PLR and outcomes in advanced lung adenocarcinoma Epidermal Growth Factor Receptor (EGFR) mutation-positive with Tyrosine Kinase Inhibitor (TKI) treatment.Methods: This retrospective study enrolled 40 medical records of lung adenocarcinoma patients treated with TKI in Ulin General Hospital from 2017-2019, with follow-up until April 1, 2021. A receiver operating curve (ROC) was performed to determine the optimal cut-off and parallel tests of NLR/PLR combination. The Kaplan-Meier was used to evaluate the impact on progressive-free survival (PFS) and overall survival (OS).Results: The optimal cut-off was 6.25 for NLR and 451.5 for PLR with sensitivity and specificity of PFS (31.6%, 100%, and 18.4%, 100%) and OS (32.4%, 100% and 8.9%, 100%) (AUC 0.362, 0.329 and 0.482, 0.477) respectively. Patients in NLR <6.25 and PLR <451.5 groups presented longer PFS (10 months, 95% CI:7.783 -12.217, vs. 8 months, 2.908-13.092, p=0.821; 10 months, 7.508 – 12.492 vs. 9 months, 6.434-11.566, p=0.513) and OS (20 months, 14.017-25.983 vs.16 months, 11.474-20.526, p=0.378; 20 months, 14.629-25.371 vs. 14 months, 3.735-24.265, p=0.382) but not significantly correlated.Conclusion: High pre-treatment NLR and PLR showed shorter PFS and OS, although they did not appear as a prognostic marker for PFS and OS of EGFR-mutant lung adenocarcinoma treated with TKI.
The Effect of Increased Glucose Induction on GSH Levels in Insulin Gaussia Luciferase (iGL) Cells Derived from Rat Pancreatic Beta Cells Ardiansah, Fery; Stujanna, Endin Nokik; Sanjaya, Arief Indra; Listiyaningsih, Erlin; Ningsih, Sri Suciati; Ujianti, Irena; Lestari, Dwi Retna
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19644

Abstract

Background: Prolonged hyperglycaemia can make the pancreatic beta cells work harder and cause fatigue. When this happens, it can trigger oxidative stress reactions, which can produce free radical compounds that can damage pancreatic beta cells. The body compensates by activating protective mechanisms such as the production of antioxidant compounds to reduce the levels of free radicals in the cells. One such compound is glutathione (GSH). Insulin Gaussia Luciferase (iGL) cells are a cell line derived from rat pancreatic β (beta) cells. These cells can be used as a model of oxidative stress in hyperglycaemia to measure GSH levels and there are no studies using iGL cells to measure GSH levels. Therefore, in this study, the iGL cells are used as the object of research.Objective: To investigate the effect of GSH levels on glucose toxicity condition through in vitro experiments on iGL cells.Methods: The study used 5 different glucose concentrations of 11, 16.5, 22, 33, and 44 mM with the addition of iGL cell growth medium exposed for 7 days. We measured the amount of intracellular GSH using a colourimetric method at a wavelength of 405 nm. The analysis used in this study was a one-way ANOVA test. Differences between groups were tested using SPSS.Results: The results of this study showed that there was an increasing trend in total GSH levels on the third and seventh day.Conclusion: there was a daily decrease in GSH/cell in the iGL sample cells exposed to different concentrations of glucose for 7 days. This can be due to an increase in the oxidative stress reactions in the cells, which can lead to a decrease in the levels of antioxidants.
Analysis Interaction of Immunoglobulin G and Immunoglobulin A Against PstS1 as a Basis Specimen Selection for M. tuberculosis Rapid Test Diagnostic Agent Rahmadani, Nabila -; Raras, Tri Yudani Mardining; Arthamin, Maimun Zulhaidah
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19431

Abstract

Background: The recombinant Ag38 protein developed from a local strain of Mycobacterium tuberculosis has great potential to be used as a sero-diagnosis agent using the antigen rapid test because it has several epitopes that bind to antibodies. However, it is not yet known which antibody Ag38-recombinant binds maximally between IgA and IgG.Objective: The aim of this study is to compare the interaction between IgG and IgA on Ag-38 rec in silico as a basis for the selection of sero-diagnosis agents in the TB rapid test.Methods: The results show that the protein PstS1 has a higher binding sensitivity to IgG based on one of the docking models which shows a docking score of -229.70, a confident score of 0.8312 and RMSD 1.060 A. The ramachandran plot also shows that testing on this model has a protein structure that is good, with disallowed regions [X,X] values of 0.5% (less than 0.8%). The results of this analysis show that the most favored regions are 90.5% with a G-factor of -0.27. The quality of the structure of the 3D mooring model can be said to be good because it fulfills the ideal structure requirements.Conclusion: Ag38-rec antigen M. tuberculosis H37Rv binds to IgG more strongly than IgA.
Comparison of SARS-CoV-2 Variant Screening and Whole Genome Sequencing at an Indonesian Tertiary Hospitals Hapsari, Rebriarina; Kesumayadi, Irfan; Sari, Desvita; Anjarwati, Dwi Utami; Alfiyuliani, Nesia Hani; Mujahidah, Mujahidah; Sari, Iva Puspita; Hadi, Purnomo
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19147

Abstract

Background: The global COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), experienced a surge in cases with the emergence of the Omicron variant. Despite increasing vaccination coverage, Indonesia witnessed peaks in COVID-19 cases. Variant screening and whole genome sequencing (WGS) play a crucial role in identifying SARS-CoV-2 variants and monitoring their spread.Objective: The objective of this study was to compare variant screening results with WGS data, assess the prevalence of subvariants, and analyze their correlation with demographic and cycle threshold (CT) values.Methods: Between November 7th and 18th, 2022, variant screening and WGS were conducted on samples with CT values below 30. Variant screening utilized the mBioCov-19+ VarScreen assay, while WGS was performed on the Oxford Nanopore Technologies (ONT) platform. Bioinformatics analysis was performed using epi2melabs. Demographic data and CT values were analyzed. Results: Out of 89 subjects, all tested positive for the Omicron variant through variant screening. The variant screening identified two subvariants: Omicron BA.2 (64%) and Omicron B.1.1.529.1 (36%). WGS revealed that the XBB subvariant was the most dominant (52.8%), followed by BQ.1 (22.5%) and BA.5 (13.5%). When VarScreen indicated BA.2, the majority of WGS results showed XBB (82.5%), while for B.1.1.529.1, the majority of WGS results were BQ.1 (59.4%), followed by BA.5 (37.5%). XBB was the most prevalent variant in both females and males, while BQ.1 was more dominant in females (80%). No infections were detected among children aged 1-5 years. All variants had CT values below 24.Conclusion: Variant screening provides accurate and quick results for detecting the Omicron variant in laboratories without WGS capacity. However, it is important to continuously update the screening methodology based on the prevailing circulating variants. During the study period, XBB emerged as the predominant subvariant of the Omicron variant.
Comparative Analysis of Kidney Histomorphometry Utilizing Two Distinct Image Processing Software Brahmadhi, Ageng; Ningrom, Ira Citra
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.18554

Abstract

ABSTRACTBackground: Histopathological examination is critical to evaluate tissue condition. An accurate assessment is necessary for diagnosis establishment. Nowadays, both quantitative and qualitative scoring are enhanced with computer-assisted image analysis to reduce bias. Various software was developed to assist in image analysis. The question of whether the measurement results from one software will be comparable to those from another software may come up, given the wide variety of software options. Nevertheless, this subject is only occasionally discussed.Objective: This study aimed to compare the measurement results from Fiji and QuPath software in kidney histomorphometry.Methods: Normal kidney histological slide was observed. Selected histological structures, including the renal corpuscle area, glomerular area, Bowman space area, inner diameter of proximal, distal, and Henle loop, were measured using QuPath and Fiji software. The measurement results from the two software were compared for value differences and agreement analysis.Results: The renal corpuscle means the area was 12.7x103 µm2 in QuPath and 12.5 x103 µm2 in Fiji. The glomerular area was 7.8 x103 µm2 for both software. The proximal tubule's inner diameters varied from 18.7 to 150.8 µm. Smaller inner diameters were observed in distal tubules (17.1-80.5 µm) and The Henle loop (15.5-69.6 µm). There was no significant difference in measurement results of particular structures between the compared software (P-value > 0.05). The further confirmational analysis supported the similarity between the two measurement results.Conclusion: the measurement result of kidney microstructures using QuPath and Fiji were identical.
Exploring Systemic Lupus Erythematosus Pathogenesis through Animal Models: A Systematic Review of Humanized and Pristane-Induced Lupus Mice Airlangga, Dimas Ikhsan; Rahmawati, Hanifa Rizky; Susianti, Hani; Handono, Kusworini
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19434

Abstract

Studies involving experimental animals to explore the pathogenesis of systemic lupus erythematosus (SLE) which leads to the selection of optimal therapy have been widely conducted. The well-known model used to study SLE includes the pristane-induced mouse model and the more recently developed humanized mouse model that implants human immune cells into immunodeficient mice. The current state of the research has yet to provide a systematic review that analyzes both model and its contribution to our understanding of SLE pathogenesis. This systematic review-based study aims to provide a comprehensive overview of the development and application of pristane-induced and humanized mouse models. We obtained several relevant article sources include: (1) Search Strategy, on databases such as PubMed, MEDLINE, ScienceDirect, and Cochrane by adjusting the protocols listed in the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA); (2) Eligibility based on exclusion and inclusion criteria; and (3) Data Extraction. The findings show that 30 articles are relevant to the subject matter. Several strains of mice were used in the model of the 0.5 pristane injection method and the humanized mice model. All studies showed similar patterns in the onset and manifestation of SLE in mice models with slight variations. The purpose of using the pristane injection method and humanized mice model is adjusted to the output of each study. A variety of research preferences can be used as a reason for choosing pristane and humanized cells transplanted SLE methods in making lupus model mice.
Analysis Of Clerodendrum inerme Plant Compounds as Anti Diabetes Mellitus Through Network Pharmacology Approach Jamil, Ahmad Shobrun; Hilmy, Fauzan
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.17607

Abstract

Background:  Diabetes mellitus prevalence in Indonesia has surged. In 2021, an estimated 19.5 million people had diabetes, with a 10.6% age-adjusted prevalence. Projections indicate around 9.5 million cases by 2024. Diabetes medications, such as metformin, are commonly used, although these medications have adverse effects. A common choice for chronic diseases like DM is the use of natural medications. A plant known as Clerodendrum inerme has the potential to alleviate diabetes, but little is known about its molecular mechanismsObjective: The purpose of this study was to explore the content of Clerodendrum inerme plant compounds and their potential for cases of Diabetes Mellitus.Methods: The KNApSAcK was used to conduct an analysis of plant parts of Clerodendrum inerme to seek out chemicals present in plants. A screening was done to find compounds by estimating Absorption, Distribution, Metabolism, and Excretion (ADME) parameters using the canonical Simplified molecular-input line-entry system (SMILES) on the SwissADME. On the SwissTargetPrediction tool, predictions of target proteins from compounds that pass screening are connected to various probable proteins. utilising the String-db  to show the network between target proteins and associated diseasesResults: The Clerodendrum inerme consist of 24 different compound. The 24 compounds were screened, and the results showed that 4 of them, specifically (Z)-3-Hexenyl beta-D-glucopyranoside, Rhodioloside, Sammangaoside B, and Clerodermic acid, had the potential to be developed into therapeutic agent. The compound are then analysed in order to find the protein target associated with diabetes mellitus and predict its networks. The findings indicate that multiple target proteins, including GSK3B, PPARG, DPP4, and STAT3, are connected to diabetes mellitus.Conclusion: It has been shown that (Z)-3-Hexenyl beta-D-glucopyranoside, or clerodermic acid, is able to attach to the proteins GSK3B, PPARG, DPP4, and STAT3, which are all linked to diabetes mellitus.
Recalcitrant Incomplete Secukinumab Administration in a Psoriasis Patient Gani Panjaitan, Joice Sonya; Suhartomi, Suhartomi
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19100

Abstract

Background: Psoriasis is an immunologic-mediated disease affected by genetic factors that may affect the skin, joints, and cardiovascular system. Some biological agents have been developed and approved by FDA to treat psoriasis. One of these biological agents is Secukinumab, a fully human IgG1κ anti-interleukin-17A(IL-17A) monoclonal antibody. Case Presentation: A seventeen female teenager came to Dermatovenerology Clinic with scaly patches in the forehead and hairline around ten months ago with a history of repeat Corticoticosteroid, DMARDs, and biologic agent treatment. Dermatology examination showed erythema, induration, and desquamation in head and extremities with PASI score of 1.2. She was treated with initial and maintenance doses of Secukinumab Injection.Conclusion: It can be concluded that the recalcitrant administration of Secukinumab in Psoriasis patients may decrease the treatment response.

Page 1 of 1 | Total Record : 8

 1 

Filter by Year

2023 2023


Reset
Filter By Issues
All Issue Vol 11, No 3 (2025): December 2025 Vol 11, No 2 (2025): August 2025 Vol 11, No 1 (2025): April 2025 Vol 10, No 3 (2024): December 2024 Vol 10, No 2 (2024): August 2024 Vol 10, No 1 (2024): April 2024 Vol 9, No 3 (2023): December 2023 Vol 9, No 2 (2023): August 2023 Vol 9, No 1 (2023): April 2023 Vol 8, No 3 (2022): December 2022 Vol 8, No 2 (2022): August 2022 Vol 8, No 1 (2022): April 2022 Vol 7, No 3 (2021): December 2021 Vol 7, No 2 (2021): August 2021 Vol 7, No 1 (2021): April 2021 Vol 6, No 3 (2020): December2020 Vol 6, No 2 (2020): Augusts 2020 Vol 6, No 1 (2020): April 2020 Vol 5, No 2 (2019): December 2019 Vol 5, No 1 (2019): July 2019 Vol 4, No 2 (2018): December 2018 Vol 4, No 1 (2018): July 2018 Vol 3, No 2 (2017): December 2017 Vol 3, No 1 (2017): July 2017 Vol 2, No 2 (2016): December 2016 Vol 2, No 1 (2016): July 2016 Vol 1, No 2 (2015): December 2015 Vol 1, No 1 (2015): April 2015 More Issue