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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Search results for , issue "Vol. 15 No. 1 (2025)" : 20 Documents clear
Combination of Diabetes Self-Management Education and Support Through Telehealth and Hypnotherapy Optimizes Self-Care Behaviors and Lowering HbA1c Levels in Patients with Type 2 Diabetes Mellitus: Combination of Diabetes Self-Management Education and Support Subiyanto, Paulus; Saifulaman, Muhammed; Sitorus, Ratna
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.03

Abstract

This study aims to explore the effectiveness of the combination of Diabetes Self-Management Education and Support (DSMES) through telehealth and hypnotherapy in optimizing self-care behavior and reducing hemoglobin A1c (HbA1c) levels in patients with type 2 diabetes mellitus (T2DM). The study used a quasi-experimental approach and comparative study with a pretest-posttest design and a 3-month cohort design. This study was conducted after obtaining a letter of ethical eligibility involving 40 T2DM patients divided into two groups. The control group received conventional medical services face-to-face. The intervention group received a DSMES program of seven self-care behaviors via telehealth with additional hypnotherapy sessions. The respondents in this study are women (60%) with an average age of 55.3 (31–65) years, who are highly educated (80%). A significant increase in DSM scores before and after the intervention occurred in the intervention group, (49.35–81.20) or (+31.85), and in the control group, (55.70–55.52) or (−0.18). The decrease in A1c in the intervention group (8.9%–7.37%) was (−1.53) and in the control group (8.12%–8.10%) was (−0.02). There were significant differences in DSM attainment and HbA1c levels in both types of interventions (p = 0.000; p = 0.030 with alpha 5%). DSMES, through telehealth combined with hypnotherapy, can provide a synergistic effect with medical treatment commonly given to patients with T2DM during and after the COVID-19 pandemic. This approach not only improves healthcare accessibility but also addresses the psychological barriers associated with lifestyle change.
Coastal Waterbirds Diversity, and Structure under Land Use Changes in the North Coastal of Situbondo, East Java: Coastal Waterbirds Diversity, and Structure under Land Use Changes in the Situbondo Coast, East Java Kartika Dewi, Yuni; Retnaningdyah, Catur; Setyo Leksono, Amin; Arisoesilaningsih, Endang
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.02

Abstract

Coast is suitable waterbirds habitat, but land use change could negatively influence the waterbirds life. The northern coast of Situbondo Regency, East Java Province, showed variations in land use quality. This study aims to analyze interaction between coastal land use changes on waterbirds diversity and community structure in the coast. Waterbird density was recorded based on the point count along the transect 100 m2 in six stations, namely Kampung Blekok, Tanjung Pecinan, Dubibir, Cemara, Banyuglugur, and Baluran National Park as the reference site. Land use quality in coastal areas was determined by satellites imagery. Habitat preference was determined by neu index. The results showed Tanjung Pecinan and Kampung Blekok stations were favourable waterbirds habitat, dominated by agriculture and low industry areas. These stasions better supported waterbird life and closed characters to Baluran one, where was dominated by mangrove forest and agriculture. This reference site showed highest diversity (H’ 2.26) and evenness (E 0.75), while preference habitat showed lower diversity (H’ 1.39) and evenness (E 0.29). On the contrary, Banyuglugur showed an industrial area, we found a low density species. Baluran and Kampung Blekok were habitat of three IUCN red list species, such as Ciconia episcopus, Charadius javanicus, and Leptoptilos javanicus.
Assessing Exogenous L-Glutamine and PEG Influence on Soybean Adventitious Roots Culture: Lateral Root Morphology and Amino Acid Profiles: L-Glutamine and PEG Influence on Adventitious Roots Culture Ardhian, Dhike; Mastuti, Retno; Nahampun, Hartinio Natalia
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.01

Abstract

Soybean (Glycine max L. Merr.) plays an important role in global agriculture and provides a primary source of plant nutrition, notably amino acids. However, tropical climates often result in lower amino acid levels than subtropical climates. This study explored the effects of L-glutamine and PEG on enhancing the amino acid content in in vitro soybean adventitious root. In this study, in vitro adventitious roots from the Detap1 soybean variety were treated in a liquid medium with various concentrations of L-glutamine (0, 1, 2, 4, 8, and 16 g.L-1) or PEG (0, 10, 20, 40, 80, and 160 g.L-1) for 8 days. The root biomass was quantified, and the amino acid profile was analyzed using HPLC. L-glutamine or PEG treatments significantly reduced root biomass by inhibiting lateral root initiation. Meanwhile, 20 amino acids were detected in the control and L-glutamine or PEG treatments. The application of a 16 g.L-1 L-glutamine significantly increased amino acid content. PEG treatments also had similar results in root growth inhibition and increased amino acid accumulation. Multivariate analysis showed significant separation between the control and treatment groups, with glutamine playing a crucial role in the group separation. This study demonstrates the potential of in vitro technology to improve amino acid production through adventitious root culture, providing insights into stress responses and metabolic regulations
Exploring Sustainable Approaches: Efficacy of Plant-Based Oils and Beauveria bassiana Fungal Spores in Managing Silverleaf Whitefly (Bemisia tabaci) Infestations: Managing Silverleaf Whitefly (Bemisia tabaci) Infestations kurnianto, agung sih; Purnomo, Hari; Sucipto , Irwanto; Muhlison , Wildan; Dewi, Nilasari; Kamalin, Indah M.; Khoiruttamam, Muhammad Zulfa; Aini, Wilda Qurrotul; Magvira , Nur Laila; Handoko, Rose Novita Sari
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

The Silverleaf whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a destructive insect pest of important greenhouse and field crops. Several studies have shown the effectiveness of Beauveria bassiana in controlling Silverleaf whitefly.. However, a major challenge lies in the rapid evaporation of water in greenhouse crop models. Therefore, this study aimed to evaluate the effectiveness of B. bassiana against the whitefly and test its compatibility with various vegetable oils as carrier media. This research at the Agrotechnology Laboratory, Faculty of Agriculture, University of Jember, used pure isolates of B. bassiana for mass production until conidia reached 2.5 x 107 per gram. We employed a Completely Randomized Design (CRD) with factors of vegetable oil (Corn Oil, Vegetable Oil, Soybean Oil) and concentration (5%, 10%, 15%) using Ultra Low Volume (ULV) formulations. The results revealed that the addition of vegetable oils enhanced the effectiveness of B. bassiana in controlling Silverleaf whitefly, with corn oil having the most significant impact due to its high carbohydrate content that supported B. bassiana viability. Additionally, the concentration of B. bassiana formulation also influenced the rate of whitefly mortality (mycocity), with a 15% concentration yielding the best results. Whitefly nymphs infected with B. bassiana exhibited slowed activity and reduced appetite. Adding vegetable oil facilitated the formation of a protective biofilm around conidia, shielding them from UV radiation, increasing conidia viability, and supporting field application. The infection process by B. bassiana required 6 days to achieve 100% mortality. This research suggests that B. bassiana can be an effective alternative for controlling Silverleaf whiteflies, especially when used with plant-based oils as a carrier medium. This approach may help reduce reliance on chemical pesticides and address issues related to Silverleaf whitefly resistance to insecticides.
Effect of Combination of Different Antibiotics and Promoters for Expressing Recombinant Darbepoetin in Stable CHO K-1 Cell Line: Evaluating Antibiotic Combinations to Improve Yield and Quality of Darbepoetin Widekdo, Dwi purno; Widodo, Nashi; Rifa'i, Muhaimin; Jatmiko, Yoga Dwi
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Antibiotics are key for successful molecular cloning techniques. Different antibiotics have different mechanisms of action, which leads to cell heath and a viable number of passages. Moreover, the suitability of the promoter also plays an important role in achieving a higher level of protein titter in the stable cell platform. Therefore, with plenty of options of antibiotics and promoters available, we need to determine the best combination of antibiotics and promoters, particularly for specific proteins of interest. Darbepoetin is a recombinant therapeutics protein with extra glycosylation to increase the half-life in the blood; this drug is used for the administration of CKD and leukemia patients. Blasticidin-S and puromycin were used as antibiotics, and CMV and EF-1 promoters were used in this experiment to evaluate the expression of recombinant darbepoetin for the protein model. CHO K-1 cell line was transfected with a plasmid carrying a combination of promoter and antibiotics genes; after 14 days, the level of specific protein expression was evaluated using the western blot technique. A single clone cell was obtained using the serial dilution method to evaluate the clonality and expression of the protein of interest. This study successfully obtained a single clone from stable pool transfection. This result suggested that a combination of puromycin antibiotics and EF-1 promoter has promising expression compared to Blasticidin-S antibiotics with CMV promoter. For further conclusion, an analytical comparison of both combinations needed to be done.
Antioxidant Activity and α-Amylase Inhibition of Methanolic Extracts from Selected Bamboo Species: Antioxidant Activity and α-Amylase Inhibition of Bamboo Shoots Ruiz, Jerome; Pascual, Pet Roey; Alburo, Rosalyn
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Bamboo contains bioactive antioxidants, which can be used to scavenge free radicals and prevent degenerative diseases, including diabetes mellitus. The objective of this study is to examine the antioxidative capacity of the bamboo shoot methanolic extracts (Bambusa blumeana, Dendrocalamus asper, and Bambusa vulgaris) as a potential antidiabetic treatment using a spectrophotometric approach. The free radical scavenging and α-amylase inhibitory capacity were measured against known standards. The EC50 was obtained to compare the effectiveness of the three bamboo extracts in reducing free radicals and α-amylase activity by 50%. Among the three extracts, Kuayan tinik (B. blumeana) has the highest total phenolic contents (467.65 ± 6.10 mg GAE/100 g sample), while giant bamboo has the highest total flavonoid contents (875.69 ± 143.47 mg QuE/100 g sample). In addition, the methanolic extract of Kuayan tinik (B. blumeana) has the most optimum antioxidant capacity based on its ability to reduce Fe-radicals (26.93 ± 3.07 mg Fe2+ /100 g sample), scavenge H2O2 (EC50 value = 69.73 ± 0.74 μg/mL), superoxide anion (EC50 value = 62.00 ± 1.27 μg/mL), and DPPH radicals (EC50 value = 57.06 ± 0.44 μg/mL). Kuayan tinik (B. blumeana) also exhibits the highest α-amylase inhibitory activity (EC50 value = 681.10 ± 45.42 μg/mL). Based on the findings, bamboo shoot methanolic extracts show potential for development into dietary supplements. Additionally, the α-amylase inhibition results suggest that bamboo shoot extracts could be further purified and explored for their potential as a diabetic treatment; however, validation through in vivo studies is recommended.
In silico Characterization of Poly (ethylene) Terephthalate (PET): Degrading Enzymes from Rhizobacter sp. for Enzymatic Degradation Mechanisms: Characterization of Rhizobacter sp. PET Hydrolases Damuri, Nur Wahida; Mohd Rozdhi, Amira Azawani; Tirmizhi Abubakar, Munkaila; Wayan Gunam, Ida Bagus; Huyop, Fahrul; Oyewusi, Habeebat Adekilekun
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Dienelactone hydrolase (DHL) from Rhizobacter sp. is an enzyme from the β‐ketoadipate pathway that belongs to the α/β hydrolase family. It involves the conversion of chloroaromatics, such as nitrophenols and hydrocarbons, into harmless metabolites. The sequence-based analysis of Dienelactone hydrolase from Rhizobacter sp. shows significant homology to the extensively studied polyethylene terephthalate hydrolase of Ideonella sakaiensis (IsPETase). IsPETase can degrade the polymer, polyethylene terephthalate (PET), at room temperature. It was chosen as a template for dienelactone hydrolase from Rhizobacter sp. that was studied as a putative PET hydrolase. This study employs bioinformatics tools such as Expasy Protparam, Clustal Omega, SWISS-MODEL, GROMACS and Autodock vina to analyse the amino acid sequence of this enzyme, predict its three-dimensional structure and study its binding interaction. The structure of the putative PET hydrolase has been determined with 0.9 GMQE value and an overall quality factor of 96%. The residues responsible in substrate binding interactions are Leu88, Ser160 and Trp185. Thus, this in silico analysis depicts the ability of the putative PET hydrolase to bind to the polymer polyethylene terephthalate.
Improvement of Home Garden Diversity and Structure by SRFG Program to Support Food Security in Pacitan Regency, Indonesia: SRFG Program to Support Food Security in Pacitan Regency, Indonesia Wijayanti, Titik; Endang Arisoesilaningsih; Yayuk Yuliati; Amin Setyo Leksono
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

The research aimed to analyze the contribution of diversity and vegetation structure of the Sustainable Reserve Food Garden (SRFG) Program in Pacitan Regency to support food security. The sampling was carried out using a purposive random sampling on 315 respondents, who were active members of the SRFG Program. Information on the condition of the SRFG home garden was obtained through interviews and questionnaires administered directly to each respondent. The SRFG home gardens were clustered into eight based on vegetation density using multivariate clustering K-Means. Spatial variations in diversity were analyzed using descriptive statistics, density of each vegetation, Shannon-Wiener index diversity, and polynomial model regression analysis using Software PAST 4.13. The research results show that clusters 1-3 had the highest density of trees (302 individuals/ha), of shrubs (11 individuals/ m2), and of herbs (46 individuals/100 m2), with a medium level of diversity (H’ trees 2.09; H’ shrubs 1.91; H’ herbs 1.88). Meanwhile, cluster 2 excelled in herbs density, with the same level of diversity. A low level of diversity (H’ 0.8) was only found in cluster 8 in that of trees. Additionally, the questionnaire results show that the impacts of food availability, food access, food utilization and nutritional health on cluster 1-3 were better than those on cluster 4-8. This is shown on a Likert’s Scale (1-4), where the means of cluster 1-3 were 3.12 in food availability, 3.74 in food access, 3.35 in food utilization and 3.65 in nutritional health. Meanwhile, cluster 4-8 indicated food availability (3.03), food access (3.69), food utilization (3.22) and nutritional health (3.52). The results implies that improving home garden diversity and structure by the SRFG program in Pacitan Regency supports food security.
In Silico and In Vitro Antibiofilm Activity of Zingiber montanum Ethyl Acetate Fraction Against Propionibacterium acnes: Antibiofilm of Zingiber montanum Aji, Nur; Kumala, Shirly; Mumpuni, Esti; Rahmat, Deni
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Propionibacterium acnes (P. acnes), a symbiotic bacterium involved in the development of acne, is recognized for its capacity to develop resistance due to its ability to generate biofilms. According to a previous investigation, the ethyl acetate fraction derived from Zingiber montanum exhibited anti-bacterial properties against P. acnes. Nevertheless, the causative role of biofilm formation in its resistance was not previously recognized. This study aims to ascertain the antibiofilm efficacy using in silico and in vivo methodologies. In this study, in silico examination utilized molecular docking to inhibit the quorum sensing mechanism by blocking the autoinducer 2 (AI-2) signal. The target protein was AI-2 binding periplasmic protein LuxP (PDB code: 4YRZ). Following that, an in vitro test was conducted using the crystal violet staining method, which was read using a microplate reader at a wavelength of 600 nm. The readings were expressed as percent inhibition and IC50. The in silico results showed that there were six compounds in the ethyl acetate fraction that have the potential to inhibit AI-2 signaling. Curcumin showed the highest inhibitory activity. Meanwhile, the in vitro results revealed that the ethyl acetate fraction of Z. montanum obtained the minimum biofilm inhibitory concentration (MBIC) of 2.5 % (w/v) and IC50 = 0.28 % (w/v). It can be concluded that the ethyl acetate fraction of Zingiber montanum could inhibit the biofilm formation of P. acnes.  
Phytochemical Analysis, Antioxidant, and Antibacterial Activity of Bidara laut (Strychnos lucida R.Br) Grown in West Bali National Park, Indonesia: Phytochemical Analysis, Antioxidant, and Antibacterial Activity of Bidara laut Krisnawati; Anita Apriliani Dwi Rahayu; Lisna Hidayati; Rizki Arisandi; Ganis Lukmandaru
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Strychnos lucida R.Br. is a medicinal plant believed to treat various diseases. The study aimed to identify the phytochemical compounds, total phenolic content (TPC), antioxidant, and antibacterial activity of the root, seed, stem, and leaf of S. lucida. Antioxidant activity was examined using the DPPH method in the methanol extract. The extract of four parts of S. lucida was tested against three bacteria (Bacillus subtilis Inacc-B334, Staphylococcus aureus Inacc-B4, and Escherichia coli Inacc-B5) using the disk diffusion method. The results showed that alkaloids and tannins were detected in all parts of the plant. The highest TPC was shown in root (6%) and the lowest TPC was found in seed extract (4.67%). The antioxidant activity test presented the value of IC50 of the methanol extract of root, seed, stem, and leaf as 129.65 µg/ml, 255.04 µg/ml, 145.28 µg/ml, and 112.96 µg/ml, respectively. Furthermore, the stem showed antibacterial activity by inhibiting the growth of B. subtilis and S. aureus in a concentration of 10% of the sample extract (v/v). Leaf extract has activity against only S. aureus growth in different concentrations (2.5, 5, 10%), while all bacteria were resistant to root and seed extracts. The stem extract showed the highest antioxidant and antibacterial activity compared to other parts.

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