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PRODUKSI ASAM LEMAK OLEAT OLEH MIKROBA ENDOFIT Sporodiobolus salimonicolor DARI TUMBUHAN KINA (Cinchona pubescens Vahl.) Mumpuni, Esti; ., Amalia; Parwati, Titi; Simanjuntak, Partomuan
Alchemy Jurnal Penelitian Kimia Vol 3, No 2 (2004)
Publisher : Alchemy Jurnal Penelitian Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Fermentasi mikroba endofit dari tannaman kina (Cinchona pubescensÂ Vahl.) dalam medium Phoma telah dilakukan. Hasil fermentasi selama 8 hari diekstraksi dengan diklormetan diperoleh asam lemak yaitu asam oleat dalam bentuk cis. Hasil ini dipertegas oleh spectra RMI (proton dan karbon) dan spektramassa (GC-MS).

Analisis Selektivitas Senyawa Turunan Diosmetin Sebagai Antioksidan Baru dengan menggunakan Metode MolecularDocking Martati, Titiek; Mumpuni, Esti; Mulatsari, Esti; Maryanto, Kenny
Jurnal Farmasi Indonesia Vol 10, No 1 (2018)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v10i1.581

Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F). Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F). Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F).

Analisis Selektivitas Senyawa Turunan Diosmetin Sebagai Antioksidan Baru dengan menggunakan Metode MolecularDocking Martati, Titiek; Mumpuni, Esti; Mulatsari, Esti; Maryanto, Kenny
Jurnal Farmasi Indonesia Vol 10, No 1 (2018)
Publisher : Jurnal Farmasi Indonesia

Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F). Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F). Free radicals are compounds that have nofree electron pair, so it ws unstable and too reactive, to be able to ward off free radicals, required an antioxidant. Antioxidant can prevent the occurrence of oxidative reactions that can cause various diseases. The commonly used antioxidant compounds are vitamin C, vitamin E, and flavonoids. One of flavonoid compound that have the potential to be developed into antioxidants is diosmetin. The results of the study using the QSAR method, state that some of diosmetin derived compounds have better antioxidant activity than diosmetin. In this research, the selectivity of diosmetin derived compounds in several enzymes as an antioxidant was tested by using molecular docking methods. Software used for molecular docking were PLANTS, YASARA, MarvinSketch. This study used 15 diosmetin derived compounds, diosmetin as a parent compound, and comparison compounds used were vitamin C, vitamin E, and quercetin. Validated target proteins are 7 (seven) receptors with PDB codes 1QQW, 1V4S, 1XAN, 2BEL, 2C9V, 4K7O, and 5M2F. The results of this study have obtained the best selective compounds to receptors and compounds in each receptor. The best compounds are 6,8-difluoro diosmetin (1QQW), 8-amine diosmetin (1V4S), 6.8-difluoro diosmetin (1XAN), 6.8-diamine diosmetin (2BEL), 5'-amine diosmetin ( 2C9V), 5'-amine diosmetin (4K7O), and 8-amine diosmetin (5M2F).

SKRINING VIRTUAL DAN ELUSIDASI MODA IKATAN SENYAWA INHIBITOR ENZIM ELASTASE DAN HYALURONIDASE PADA BEBERAPA TANAMAN DENGAN AKTIVITAS ANTI-AGING Mumpuni, Esti; Mulatsari, Esti; Noerfa, Tri Kumala
Jurnal Farmasi Indonesia Vol 11, No 2 (2019)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v11i2.677

Several studies on elastase enzymes and hyaluronidase enzymes that have the potential to be anti-aging have been previously published. Referring to the various results of the study, a virtual screening of 30 natural substances from 10 anti-aging plants and the binding mode elucidation of the active compound as an inhibitor of enzyme causing aging were carried out. This study aims to determine the inhibiting mechanism of antiaging compounds in plants and to model their interactions. Based on the results of the ChemPLP score from the docking simulation there were 4 active compounds was obtained which inhibited the elastase enzyme, namely : 2 compounds from orange plants (Citrus aurantium subsp. Amara), 1 compound from lavender flower (Lavandula angustifolia L.) and 1 compound from galangal striped plants (Alpinia zerumbet). While on the hyaluronidase enzyme obtained 11 compounds that actively inhibit the hyaluronidase enzyme there were 2 compounds from the galangal striped plant (Alpinia zerumbet), 3 compounds from the areca nut plant (Areca catechu L.) , 1 compound from the tea plant (Camellia sinensis Kuntze), 1 compound from the rose flower (Rosa centifolia L.) , 2 compounds from the orange plant (Citrus aurantium subsp. Amara), 1 compound from lavender (Lavandula angustifolia L.) and 1 compound from the seaweed (Eucheuma spinosum).

ANALISIS QSAR SENYAWA TURUNAN DIOSMETIN SEBAGAI ANTIOKSIDAN BARU DENGAN METODE SEMI EMPIRIK AM 1 Mulatsari, Esti; Martati, Titiek; Mumpuni, Esti; Hidayat, Andika Muhammad
Jurnal Farmasi Indonesia Vol 11, No 2 (2019)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v11i2.672

Diosmetin is a bioflavonoid that abundant in nature and it is flavone derived compound that have low antioxidant activity so the utilization of diosmetin as an antioxidant is very rare. To optimize the utilization of diosmetin as an antioxidant, diosmetin chemical structure was developed using QSAR methode. Quantitative Structure and Activity Relationship (QSAR) analysis of 195 diosmetin derived compound have been performed using electronic descriptors i.e heat formation, HOMO energy, LUMO energy, Log P value, mass, C2, C4, and C4?. The electronic structure as descriptor were analyzed using Hyperchem 8.0.6 software for Windows 7.0 using AM1 semi-empirical method. The descriptors were obtained by molecular modelling to obtain the most stabile structure after geometry optimation process. The best QSAR equation model was determined by multilinier regression approach. The best QSAR equation is ?log IC50 = 70.52781 + (-0.0298 Panas Pembentukan) + (3.627483 Energi HOMO) + (-0.48695 Energi LUMO) + (0.274203 Energi Hidrasi) + (-3.43515 Log P) + (-0.17476 Massa) + (26.95107 muatan atom C2) + (-3.67587 muatan atom C4) + (-3.85701 muatan atom C4?). The result of the IC50 prediction value showed that 3,6-diamin diosmetin is a diosmetin derived compound that have the best antioxidant activity with IC50 value 0.33 µg/mL.

SINTESIS SENYAWA 1,5-BIS(3â€™-ETOKSI-4â€™-HIDROKSIFENIL)-1,4-PENTADIEN-3-ON (EHP) DENGAN BAHAN BAKU ETIL VANILIN MUTU TEKNIK Mulatsari, Esti; Mumpuni, Esti; Purwanggana, Agus; Rasdianti, Putri
Jurnal Zarah Vol 9 No 1 (2021): Jurnal Zarah
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31629/zarah.v9i1.2184

Selama ini, sintesis senyawa 1.5-bis (3'-etoksi-4'-hidroksifenil)-1,4-pentadien-3-on (EHP) menggunakan etil vanillin kualitas pro analisis sebagai bahan baku. Dalam penelitian ini, dilakukan sintesis EHP dengan etil vanilin kualitas teknis sebagai bahan baku. Tujuan dari penelitian ini adalah memperoleh rendemen yang tinggi dengan karakteristik EHP yang sama dengan hasil sintesis menggunakan etil vanillin pro analisis akan tetapi biaya produksi lebih rendah. EHP merupakan senyawa analog kurkumin dengan mengubah gugus metoksi menjadi etoksi dan diketon menjadi monoketon. Metode sintesis menggunakan reaksi kondensasi aldol dengan variasi waktu kondensasi. Karakterisasi dan identifikasi senyawa hasil sintesis dilakukan menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa rendemen optimum yang diperoleh adalah 73,23% dalam waktu kondensasi 7 hari. Proses karakterisasi dan identifikasi menunjukkan bahwa senyawa yang disintesis berwarna hijau kecoklatan dan memiliki aroma aromatik, titik leleh 105,2-109,8 Â°C, Faktor retardasi (Rf) 0,68 dengan uji Kromatografi Lapis Tipis, serapan maksimum 310 dan 278,50 nm dengan uji kromatografi UV-Vis, vibrasi pada bilangan gelombang 3354,38 cm-1; 2979,82 dan 2931,60 cm-1; 1576.99 cm-1; 1672,51 cm-1; 838,59 dan 631,78 cm-1 dengan uji spektrofotometri Inframerah dan persentase area kemurnian dengan uji densitometri 76,69% dan persentase rendemen 51,16 %. Hasil ini menunjukkan karakteristik dan rendemen yang sama dibandingkan dengan EHP yang disintesis menggunakan etil vanilin kualitas pro analisis sebagai bahan baku dengan persentase rendemen yang lebih rendah.

The Demineralization Optimization for Chitosan Synthesis from Crab Shell Waste (Portunus pelagicus) Sari, Intan Permata; Mulatsari, Esti; Mumpuni, Esti; Purwanggana, Agus
JURNAL FARMASI DAN MAKANAN Vol 7 No 2 (2024): Journal of Pharmacy and Science
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/jops.v7i2.4653

Chitosan, gelatin, albumin, and sodium alginate are examples of natural polymers that are often utilized as a basis material for polymeric nanoparticles. The deacetylation of chitin molecules produces the formation of chitosan. Chitin, protein, CaCO3, MgCO3, and astaxanthin pigment are all found in crab shells. Crab shell is an undervalued potential waste. Despite the fact that copious crab shell waste can be used to produce raw materials and industrial products. According to the findings of Mohadi et al (2014), chitosan was extracted with a yield of 70.71% and a deacetylation degree of 76.6%. Previous research on optimizing chitosan synthesis has involved changing the base reagent at the deacetylation stage. The results showed that a 50% concentration of KOH reagent produced the best chitosan properties. Another study on the synthesis of chitosan from crab shells got a yield of 70.4%, and the following analysis showed that this high yield is due to the amount of calcium. Therefore, demineralization in the synthesis of chitosan from crab shells must be optimized. The calcium content was measured after optimization with various solvent concentration variations. The best demineralization optimization results use 3 M hydrochloric acid with a decrease in calcium content of 97.75%.

In Silico Study of Inhibition of Activation Of 1A52 Ethanol Extract Leunca Fruit (Solanum nigrum L) Fajar, In Rahmi Fatria; Anwar, Effionora; Mumpuni, Esti; Desmiaty, Yesi; Agustini, Kurnia
SANITAS: Jurnal Teknologi dan Seni Kesehatan Vol 15 No 2 (2024): SANITAS Volume 15 Nomor 2 Tahun 2024
Publisher : Politeknik Kesehatan Kemenkes Jakarta II

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36525/sanitas.2024.516

The development of science has found a way to prevent skin aging in menopausal women. One of the therapies is estrogen replacement therapy (17β estradiol). Skin aging in menopausal women is associated with decreased ERα expression. Leunca fruit (Solanum nigrum L) is known to contain phytoestrogens and has the potential as an antiaging agent. The purpose of this study was to predict the anti-aging effect of Solanum nigrum ethanol extract compounds through the Erα 1A52 [A] activation pathway. The research method of active substance analysis metabolite profiling was carried out by analyzing physicochemical properties using pkCSM. This analysis was carried out as an initial selection of compound safety for therapy. Molecular docking with the MVD (Free trial) application. The RMSD value ≤ 2Å indicates a valid docking and the application can be used. The results of the in silico study showed that the results of metabolite profiling showed that diosgenin was an agonist against ERα. The compound has physicochemical properties according to the Lipinski standard so that it can bind to ERα. The conclusion is that diosgenin has the potential and can act as a 1A52 [A] inhibitor so it can be used as an antiaging agent.

Analisis In Silico Senyawa Curcuma xanthorrhiza dan Moringa oleifera sebagai Kandidat Antiinflamasi Syamsi, Lusi Nursilawati; Syamsudin, Syamsudin; Mumpuni, Esti; Widowati, Wahyu; Nursanti, Okta; Qodriah, Rahmatul
Journal Syifa Sciences and Clinical Research (JSSCR) Vol 7, No 1 (2025): Volume 7 Edisi 1 2025
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/jsscr.v7i1.30385

Inflammation is the body's immune response to pathogens, tissue injury, or exposure to toxic substances. The search for effective anti-inflammatory agents remains a key focus in pharmaceutical and healthcare research. Temulawak (Curcuma xanthorrhiza) and Moringa (Moringa oleifera) have been widely used in traditional medicine due to their bioactive compounds with potential anti-inflammatory effects. This study aims to evaluate the molecular interactions between curcumin from temulawak, as well as quercetin and kaempferol from moringa, against inflammatory targets using an in silico approach through molecular docking and molecular dynamics simulations.

Docking Molekular dari Trigonella foenum-graceum sebagai Antidiabetes menggunakan Molegro Virtual Docking Prasetiyo, Andri; Mumpuni, Esti; Tjandrawinata, Raymond R
Jurnal Jamu Indonesia Vol. 4 No. 2 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v4i2.132

Trigonella foenum-graceum atau fenugreek digunakan secara luas sebagai obat tradisional untuk pengobatan diabetes tetapi mekanisme kerjanya masih belum jelas. Penelitian bertujuan memprediksi senyawa dalam fenugreek yang berkhasiat sebagai antidiabetes secara in-silico dengan menggunakan perangkat lunak Molegro Virtual Docking . Docking dilakukan 10 Senyawa uji dalam fenugreek yaitu 4-hidroxyisoleucine, coumarine, diosgenin, galactomannan, isovitexin, quarcetin, tigogenin, trigoneline, vitexin dan yamogenin dengan 3 reseptor yaitu sugar beet alpha-glucosidase- (PDB ID : 3W37), human dipeptidyl peptidase-4 (PDB ID : 1X70), human peroxisome proliferator activated gamma (PDB: 2PRG) serta senyawa pembanding acarbose, sitagliptin dan rosiglitazone. Dari 10 senyawa uji, galactomanann memiliki nilai Rerank Score/RS paling rendah di dua reseptor yaitu alpha glucosidase dan peroxisome proliferator activated gamma dengan nilai berturut turut -116.56 kcal/mol dan -131.18 kcal/mol dan nilai RS acarbose -113.60 kcal/mol dan rosiglitazone -124.54 kcal/mol . Dari 10 senyawa uji, tigogenin memiliki nilai RS paling rendah direseptor dipeptidyl peptidase-4 dengan nilai RS -86.54 kcal/mol dan nilai RS sitagliptin -87.02 kcal/mol. Berdasarkan nilai RS, galactomannan diprediksi memiliki aktivitas antidiabetes yang bekerja pada reseptor alpha-glucosidase dan peroxisome proliferator activated gamma sedangkan tigogenin diprediksi memiliki aktivitas antidiabetes yang bekerja pada reseptor dipeptidyl peptidase-4.

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