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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
 88   89   90   91   92 
Authentication of Barbonymus spp. From Lake Singkarak Using DNA Barcoding: Authentication of Barbonymus spp. From Lake Singkarak using DNA Barcoding Salis, Viola Mutiara; Roesma, Dewi Imelda; Tjong, Djong Hon; Syaifullah; Aadrean; Dahelmi
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.15

Abstract

The local community distinguishes between Barbonymus belinka (Balingka) and Barbonymus schwanefeldii (Kapiek) in Lake Singkarak based on size due to the morphological similarities between the two species. From previous reports, B. belinka (Balingka) is a fish endemic to Lake Singkarak, West Sumatra, while B. schwanefeldii has a wider distribution, including Sumatra, Kalimantan, and Java. Consequently, molecular identification is necessary to discern between the species and to understand the DNA barcode characteristics of fish belonging to the genus Barbonymus in Lake Singkarak. One molecular technique utilized for species identification is DNA barcoding, which focuses on the COI (Cytochrome Oxidase Subunit I) gene. Liver tissue samples from Balingka and Kapiek fish from Lake Singkarak were used in the study. Based on 585 bp of COI gene sequences and 30 comparison sequences from BOLD system and GenBank NCBI, seven samples from Lake Singkarak show a genetic distance of 0–1.2% from B. schwanefeldii populations elsewhere, with 15 differing nucleotide bases. Moreover, samples from Lake Singkarak show a genetic distance of 7.7–8.2% from B. belinka in the BOLD system from Aceh, with 42 differing nucleotide bases. Furthermore, two specific bases are present in B. schwanefeldii from Lake Singkarak. Based on the results of this research, it is known that all samples from Lake Singkarak, including Balingka and Kapiek, belong to the same species, namely B. schwanefeldii.
Combination of Diabetes Self-Management Education and Support Through Telehealth and Hypnotherapy Optimizes Self-Care Behaviors and Lowering HbA1c Levels in Patients with Type 2 Diabetes Mellitus: Combination of Diabetes Self-Management Education and Support Subiyanto, Paulus; Saifulaman, Muhammed; Sitorus, Ratna
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.03

Abstract

This study aims to explore the effectiveness of the combination of Diabetes Self-Management Education and Support (DSMES) through telehealth and hypnotherapy in optimizing self-care behavior and reducing hemoglobin A1c (HbA1c) levels in patients with type 2 diabetes mellitus (T2DM). The study used a quasi-experimental approach and comparative study with a pretest-posttest design and a 3-month cohort design. This study was conducted after obtaining a letter of ethical eligibility involving 40 T2DM patients divided into two groups. The control group received conventional medical services face-to-face. The intervention group received a DSMES program of seven self-care behaviors via telehealth with additional hypnotherapy sessions. The respondents in this study are women (60%) with an average age of 55.3 (31–65) years, who are highly educated (80%). A significant increase in DSM scores before and after the intervention occurred in the intervention group, (49.35–81.20) or (+31.85), and in the control group, (55.70–55.52) or (−0.18). The decrease in A1c in the intervention group (8.9%–7.37%) was (−1.53) and in the control group (8.12%–8.10%) was (−0.02). There were significant differences in DSM attainment and HbA1c levels in both types of interventions (p = 0.000; p = 0.030 with alpha 5%). DSMES, through telehealth combined with hypnotherapy, can provide a synergistic effect with medical treatment commonly given to patients with T2DM during and after the COVID-19 pandemic. This approach not only improves healthcare accessibility but also addresses the psychological barriers associated with lifestyle change.
Coastal Waterbirds Diversity, and Structure under Land Use Changes in the North Coastal of Situbondo, East Java: Coastal Waterbirds Diversity, and Structure under Land Use Changes in the Situbondo Coast, East Java Kartika Dewi, Yuni; Retnaningdyah, Catur; Setyo Leksono, Amin; Arisoesilaningsih, Endang
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.02

Abstract

Coast is suitable waterbirds habitat, but land use change could negatively influence the waterbirds life. The northern coast of Situbondo Regency, East Java Province, showed variations in land use quality. This study aims to analyze interaction between coastal land use changes on waterbirds diversity and community structure in the coast. Waterbird density was recorded based on the point count along the transect 100 m2 in six stations, namely Kampung Blekok, Tanjung Pecinan, Dubibir, Cemara, Banyuglugur, and Baluran National Park as the reference site. Land use quality in coastal areas was determined by satellites imagery. Habitat preference was determined by neu index. The results showed Tanjung Pecinan and Kampung Blekok stations were favourable waterbirds habitat, dominated by agriculture and low industry areas. These stasions better supported waterbird life and closed characters to Baluran one, where was dominated by mangrove forest and agriculture. This reference site showed highest diversity (H’ 2.26) and evenness (E 0.75), while preference habitat showed lower diversity (H’ 1.39) and evenness (E 0.29). On the contrary, Banyuglugur showed an industrial area, we found a low density species. Baluran and Kampung Blekok were habitat of three IUCN red list species, such as Ciconia episcopus, Charadius javanicus, and Leptoptilos javanicus.
Assessing Exogenous L-Glutamine and PEG Influence on Soybean Adventitious Roots Culture: Lateral Root Morphology and Amino Acid Profiles: L-Glutamine and PEG Influence on Adventitious Roots Culture Ardhian, Dhike; Mastuti, Retno; Nahampun, Hartinio Natalia
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.15.01.01

Abstract

Soybean (Glycine max L. Merr.) plays an important role in global agriculture and provides a primary source of plant nutrition, notably amino acids. However, tropical climates often result in lower amino acid levels than subtropical climates. This study explored the effects of L-glutamine and PEG on enhancing the amino acid content in in vitro soybean adventitious root. In this study, in vitro adventitious roots from the Detap1 soybean variety were treated in a liquid medium with various concentrations of L-glutamine (0, 1, 2, 4, 8, and 16 g.L-1) or PEG (0, 10, 20, 40, 80, and 160 g.L-1) for 8 days. The root biomass was quantified, and the amino acid profile was analyzed using HPLC. L-glutamine or PEG treatments significantly reduced root biomass by inhibiting lateral root initiation. Meanwhile, 20 amino acids were detected in the control and L-glutamine or PEG treatments. The application of a 16 g.L-1 L-glutamine significantly increased amino acid content. PEG treatments also had similar results in root growth inhibition and increased amino acid accumulation. Multivariate analysis showed significant separation between the control and treatment groups, with glutamine playing a crucial role in the group separation. This study demonstrates the potential of in vitro technology to improve amino acid production through adventitious root culture, providing insights into stress responses and metabolic regulations
Exploring Sustainable Approaches: Efficacy of Plant-Based Oils and Beauveria bassiana Fungal Spores in Managing Silverleaf Whitefly (Bemisia tabaci) Infestations: Managing Silverleaf Whitefly (Bemisia tabaci) Infestations kurnianto, agung sih; Purnomo, Hari; Sucipto , Irwanto; Muhlison , Wildan; Dewi, Nilasari; Kamalin, Indah M.; Khoiruttamam, Muhammad Zulfa; Aini, Wilda Qurrotul; Magvira , Nur Laila; Handoko, Rose Novita Sari
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

The Silverleaf whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a destructive insect pest of important greenhouse and field crops. Several studies have shown the effectiveness of Beauveria bassiana in controlling Silverleaf whitefly.. However, a major challenge lies in the rapid evaporation of water in greenhouse crop models. Therefore, this study aimed to evaluate the effectiveness of B. bassiana against the whitefly and test its compatibility with various vegetable oils as carrier media. This research at the Agrotechnology Laboratory, Faculty of Agriculture, University of Jember, used pure isolates of B. bassiana for mass production until conidia reached 2.5 x 107 per gram. We employed a Completely Randomized Design (CRD) with factors of vegetable oil (Corn Oil, Vegetable Oil, Soybean Oil) and concentration (5%, 10%, 15%) using Ultra Low Volume (ULV) formulations. The results revealed that the addition of vegetable oils enhanced the effectiveness of B. bassiana in controlling Silverleaf whitefly, with corn oil having the most significant impact due to its high carbohydrate content that supported B. bassiana viability. Additionally, the concentration of B. bassiana formulation also influenced the rate of whitefly mortality (mycocity), with a 15% concentration yielding the best results. Whitefly nymphs infected with B. bassiana exhibited slowed activity and reduced appetite. Adding vegetable oil facilitated the formation of a protective biofilm around conidia, shielding them from UV radiation, increasing conidia viability, and supporting field application. The infection process by B. bassiana required 6 days to achieve 100% mortality. This research suggests that B. bassiana can be an effective alternative for controlling Silverleaf whiteflies, especially when used with plant-based oils as a carrier medium. This approach may help reduce reliance on chemical pesticides and address issues related to Silverleaf whitefly resistance to insecticides.
Effect of Combination of Different Antibiotics and Promoters for Expressing Recombinant Darbepoetin in Stable CHO K-1 Cell Line: Evaluating Antibiotic Combinations to Improve Yield and Quality of Darbepoetin Widekdo, Dwi purno; Widodo, Nashi; Rifa'i, Muhaimin; Jatmiko, Yoga Dwi
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Antibiotics are key for successful molecular cloning techniques. Different antibiotics have different mechanisms of action, which leads to cell heath and a viable number of passages. Moreover, the suitability of the promoter also plays an important role in achieving a higher level of protein titter in the stable cell platform. Therefore, with plenty of options of antibiotics and promoters available, we need to determine the best combination of antibiotics and promoters, particularly for specific proteins of interest. Darbepoetin is a recombinant therapeutics protein with extra glycosylation to increase the half-life in the blood; this drug is used for the administration of CKD and leukemia patients. Blasticidin-S and puromycin were used as antibiotics, and CMV and EF-1 promoters were used in this experiment to evaluate the expression of recombinant darbepoetin for the protein model. CHO K-1 cell line was transfected with a plasmid carrying a combination of promoter and antibiotics genes; after 14 days, the level of specific protein expression was evaluated using the western blot technique. A single clone cell was obtained using the serial dilution method to evaluate the clonality and expression of the protein of interest. This study successfully obtained a single clone from stable pool transfection. This result suggested that a combination of puromycin antibiotics and EF-1 promoter has promising expression compared to Blasticidin-S antibiotics with CMV promoter. For further conclusion, an analytical comparison of both combinations needed to be done.
Antioxidant Activity and α-Amylase Inhibition of Methanolic Extracts from Selected Bamboo Species: Antioxidant Activity and α-Amylase Inhibition of Bamboo Shoots Ruiz, Jerome; Pascual, Pet Roey; Alburo, Rosalyn
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

Bamboo contains bioactive antioxidants, which can be used to scavenge free radicals and prevent degenerative diseases, including diabetes mellitus. The objective of this study is to examine the antioxidative capacity of the bamboo shoot methanolic extracts (Bambusa blumeana, Dendrocalamus asper, and Bambusa vulgaris) as a potential antidiabetic treatment using a spectrophotometric approach. The free radical scavenging and α-amylase inhibitory capacity were measured against known standards. The EC50 was obtained to compare the effectiveness of the three bamboo extracts in reducing free radicals and α-amylase activity by 50%. Among the three extracts, Kuayan tinik (B. blumeana) has the highest total phenolic contents (467.65 ± 6.10 mg GAE/100 g sample), while giant bamboo has the highest total flavonoid contents (875.69 ± 143.47 mg QuE/100 g sample). In addition, the methanolic extract of Kuayan tinik (B. blumeana) has the most optimum antioxidant capacity based on its ability to reduce Fe-radicals (26.93 ± 3.07 mg Fe2+ /100 g sample), scavenge H2O2 (EC50 value = 69.73 ± 0.74 μg/mL), superoxide anion (EC50 value = 62.00 ± 1.27 μg/mL), and DPPH radicals (EC50 value = 57.06 ± 0.44 μg/mL). Kuayan tinik (B. blumeana) also exhibits the highest α-amylase inhibitory activity (EC50 value = 681.10 ± 45.42 μg/mL). Based on the findings, bamboo shoot methanolic extracts show potential for development into dietary supplements. Additionally, the α-amylase inhibition results suggest that bamboo shoot extracts could be further purified and explored for their potential as a diabetic treatment; however, validation through in vivo studies is recommended.
The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress Turhadi, .; Hamim, .; Ghulamahdi, Munif; Miftahudin, .
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.10

Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
The Impact of Caffeic Acid Phenethyl Ester on Spinal Cord Inflammation in Mice Model of Multiple Sclerosis: Impact of Caffeic Acid Phenethyl Ester in Multiple Sclerosis Alnawajha , Amin; Endharti , Agustina; Santoso , Sanarto; Santosaningsih , Dewi
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.18

Abstract

Since the majority of the current therapies lack effectiveness and efficiency in treating Multiple Sclerosis, in addition to their high cost, monitoring during usage, and the serious side effects associated with using this therapy, which in some cases may be fatal, for these reasons, there is a necessary need for effective therapy in the clinical setting and searching for an alternative therapy that is effective and safe. For this purpose, this study evaluated the impact and efficiency of Caffeic acid phenethyl ester(CAPE) in the amelioration of inflammation and demyelination in the spinal cord of experimental autoimmune encephalomyelitis(EAE) mouse model multiple sclerosis, which could be a candidate therapy for MS. Multiple sclerosis is an autoimmune T-cell mediated disease, that T- cells become active, and differentiate into Th subset.α4β1integrin increased on the surface of T- cell during inflammation, which regulates immune cell cross through the blood-brain barrier into the central nervous system, and causes inflammation in the brain and spinal cord, myelin sheath damage and neuron demyelination. The in-vivo experiment used mice. The twenty-five mice were divided into control negative, control positive, and three treatment groups. After this, EAE was induced in mice by injecting myelin oligodendrocyte glycoprotein peptide. The mice were monitored and scored daily for clinical signs. CAPE was orally administered to mice at 5 mg/kg for T1, 10 mg/kg for T2, and 20 mg/kg for T3 for 14 days. Immunofluorescence was used to assess α4integrin, Immunohistochemistry (IHC) was used to evaluate infiltration of CD3-T cell marker, and Luxol Fast Blue stain was used to evaluate demyelination.  We found that CAPE treated mice model had a reduced infiltration of immune cells, demyelination in the spinal cord mice model, and decreasing α4integrin expression. These findings strongly demonstrated that CAPE could be a potential therapy for Multiple sclerosis, as it ameliorated the inflammation and demyelination in mice models.
Characterization of Starch Granaule of Local Water Yam (Dioscorea alata L.) from Lermatang, Tanimbar Island District, Maluku: Characterization of Starch Granule of Dioscorea alata L. Sinay, Hermalina; Karuwal, Ritha Lusian; Fauziah, Fauziah; Anaktototy , Helena
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.18

Abstract

Most tuberous crops, for instance, the yam, whose scientific name is Dioscorea alata, have food reserves in the form of starch in tubers. Characterization of starch grains of a plant species is pertinent to uncover information on plant taxonomic traits and identify their potential use as food and industrial raw materials. The study aimed to determine the characteristics of starch grains of water yam from Lermatang village, Tanimbar Islands Regency. This study isolated and submitted six accessions of water yam in Lermatang Village. The morphological inspection of the plants used the Descriptor for Yam from IPGRI, and sample preparation for observing starch grains employed the fresh section method. The six accessions of D. alata found in Lermatang village, Tanimbar Islands, were Uwi Merah, Uwi Gula, Uwi Pingingsian, Uwi Babulu, Uwi Akiakab, and Uwi Petatas. These yam varieties show differences in their tubers' shape and colors. The study found that starch granules of D. alata in each accession varied in terms of granule shape and hilus type. The starch granules revealed dominant triangular, oval, and elliptical shapes and eccentric and concentric hilus types. The starch grain diameter in the six accessions of Uwi was categorically large (44.88 – 57.5 µm), with Uwi Petatas being the largest. Previous reports have shown that accessions having large starch granule types could be further developed into an array of foods, thus conveying the promising use of the Uwi Petatas for such a purpose.

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