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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
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In Vitro Study of Garcinia dulcis (Roxb.) Kurz Leaves Extract against Hepatitis C Virus: Garcinia dulcis Leaves Extract against Hepatitis C Virus Apriyanto, Dadan Ramadhan; Hartati, Sri; Sri Wahyuni, Tutik; Inge Lusida, Maria
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.20

Abstract

Garcinia dulcis is a plant commonly used in traditional medicine in tropical regions. It has anti-bacterial, anti-inflammatory, anti-tumor, and anti-malaria properties. Despite its potential benefits, studies on its effectiveness as an antiviral are limited. This study investigated the antiviral properties of G. dulcis leaf extract against the JFH-1 strain genotype 2a as a treatment for Hepatitis C Virus (HCV). Huh7it-1 cells infected with the HCV genotype 2a strain JFH-1 were used to determine the antiviral effect of methanol extracts of G. dulcis leaves (GD-LE). GD-LE antiviral activity was investigated using a focus-forming assay for anti-HCV and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay for cytotoxicity. A time-of-addition study of anti-HCV was also performed to determine the mode of action of GD-LE. The results showed that GD-LE inhibited HCV replication with an IC50 of 17.06 µg/mL without giving any toxicity to the cells. The mode-of-action of GD-LE was found to inhibit HCV replication in the co-addition and post-infection stages. These findings suggest that GD-LE could be a promising candidate for anti-HCV treatment. However, further study regarding GD-LE bioactive compound isolation needs to be done to prepare an effective antiviral.
Extract of Black Rice (Oryza sativa L. ‘Sembada Hitam’) Bran Protect Cytotoxicity of Hydrogen Peroxide on Vero Cells in a Short Time Incubation: Black rice bran ‘Sembada Hitam’ protect cytotoxicity of H2O2 Aprilianti, Suci Hari; Purwestri, Yekti Asih; Saragih, Hendry T.S.S.G; Nuriliani, Ardaning
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.01

Abstract

Oxidative stress induced by hydrogen peroxide (H2O2) can lead to cellular damage, contributing to degenerative diseases and aging. Black rice bran is a functional food known for its antioxidant properties, which are crucial in reducing the adverse effects of oxidative stress and maintaining redox balance. In this study, we aimed to investigate the protective effect of the extract of black rice bran (EBRB) 'Sembada Hitam' on Vero cells against H2O2 toxicity. To evaluate the protective effect, a co-culture method was employed, and cell viability was assessed using the MTT assay. Additionally, cell growth was examined through trypan blue staining. Vero cells were exposed to different concentrations of H2O2 and EBRB for a 24-hour period. The results demonstrated that EBRB at concentrations of 15.625, 250, and 500 μg/mL exhibited a protective effect on Vero cells exposed to H2O2 at concentrations of 100, 200, and 400 μM, respectively. Notably, when Vero cells were treated with EBRB at concentrations of 250 or 500 μg/mL for five days in conjunction with H2O2 exposure at concentrations of 200 or 400 μM for 24 hours, a significant decrease in cell viability was observed on day 3. Based on the collective findings, it can be concluded that EBRB has the potential to protect Vero cells against H2O2 -induced toxicity, but primarily during a short-term incubation period. Overall, this study highlights the protective properties of EBRB against H2O2 -induced cellular damage and emphasizes the importance of further investigations to fully elucidate the underlying mechanisms and potential long-term effects of EBRB on cell viability.
Validation of Hand Grading Technique of Corpora Lutea in Embryo Recipient Cows: Accuracy in Hand Grading of Corpora Lutea in Cows Perera, Gamage Dammika Rohan Kumara; Pushpakumara, Pupulewatte Gedara Anil; Alexander, Ponvila Arachchilage Basil Doglas
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.09

Abstract

The success of embryo transfer in cattle may depend on several factors. The blood progesterone level of the embryo recipient would be one of the main factors secondary to the size and quality of the corpus luteum. Per-rectal palpation and by-hand grading of corpora lutea have been practiced routinely, even under commercial embryo transfer programs in cattle. This experiment aimed to evaluate the accuracy of the per-rectal hand grading technique of mid-phase corpora lutea in embryo recipient cows. A group of healthy cows (Friesian× Jersey, n=25), which had been selected as recipient animals with the special consideration of reproductive history in an embryo transfer program, was used in this study. Estrous of those animals were synchronized using a standard progesterone intravaginal devise-PGF2α protocol. Conventional evaluation and grading of corpora lutea were conducted at the per-rectal hand grading as 'A', 'B', 'C' and 'Cystic' (If diameters; < 1cm=C, 1-1.9cm=B, 2-2.5cm= A, >2.5cm= Cystic). In this study, the accuracy of those hand-grading techniques was evaluated using ultra-sound scanning technology on the same day. Identification accuracy of grades C, B, A, and Cysts in hand grading technique was 45%, 56%, 67%, and 50%, respectively. The average accuracy in the hand grading technique was 52%, and identification accuracy as suitable (A or B) corpora lutea for embryo transfer was 76%. Results raised the 24% probable loss of embryo transferrable recipients due to the hand-grading technique. Further, 8% of unsuitable cows can be selected as embryo recipients due to the practice of hand-grading techniques at embryo transfer programs. Although higher accuracy is a characteristic of the hand grading technique, the technology causes considerable loss due to inaccurate selection probability of recipients at embryo transfer programs.
Chemical Compounds and Antibacterial Activity of Thymus Vulgaris Leaves’ Ethanolic Extract Against Salmonella typhimurium: Thymus vulgaris Against Salmonella typhimurium Abousouh, Emad Khaleefah; Endharti, Agustina Tri; Santoso, Sanarto; Santosaningsih, Dewi
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.09

Abstract

Invasive non-typhoidal Salmonella is an important pathogen that causes life-threatening bloodstream infections. Antibiotic resistance in non-typhoidal Salmonella has emerged as a result of the increasing use of antibiotics; therefore, an alternative source of therapeutic agents is required. This study aimed to investigate the active compound and antibacterial activity of Thymus vulgaris leaves’ ethanolic extract against Salmonella typhimurium. Thin-layer chromatography was performed to identify the active compound in the ethanolic extract of T. vulgaris leaves. The antibiotic effect of the extract was carried out by agar dilution assay using the following concentrations: 0%, 2.5%, 5.0%, 7.5%, 10.0%, 12.5%, and 15.0% (w/v). Thymol and carvacrol were detected in the thin-layer chromatography. The minimum inhibitory concentration of the extract was 10.0% (w/v). In conclusion, T. vulgaris leaves’ ethanolic extract demonstrated antimicrobial activity against S. typhimurium. Further investigation is required to analyze the role of thymol and carvacrol as active compounds against S. typhimurium.
The Correlation of Avifauna Diversity and Its Habitat: An Observation Study at Mount Kawi Slope Waterfall, East Java: Correlation of Avifauna Diversity and Its Habitat Lutfita Fitriana; Nia Kurniawan; Amin Setyo Leksono
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.11

Abstract

Avifauna inhabiting mountain forest ecosystems on Java Island face severe threats from anthropogenic disturbances. Despite this, the diversity of avifauna in specific areas, such as the Hutan Lindung Pegunungan Putri Tidur (HLPPT) on the slopes of Mount Kawi, remains poorly monitored. This study focuses on assessing avifauna diversity and communities in the tourist area of Mount Kawi slope waterfalls, situated on both the southeast side in Malang Regency and the north side in Batu City. The research, conducted from January to March 2022, examined eight waterfall locations (Baung, Glotak, Luksongo, Parangtejo, Manten, Tengah, Rondo, and Sumberpitu) using the roaming method with Visual Encounter Survey (VES) and Audiovisual Encounter Survey (AES) techniques. Data analysis included considerations of conservation status, local distribution, feed specialists, diversity indices (Taxa Richness, Shannon-Wiener Diversity Index (H’), Simpson Index (D), Evenness Index (E)), and Important Value Index (IVI) as an assessment of avifaunal diversity. Researchers employed Microsoft Excel and Principal Component Analysis (PCA) to determine preferences for each character diversity index and abiotic factors in each location. Across the site, 84 species from 33 families were identified. Notably, seven species were classified as at risk (4 near-threatened, two vulnerable, and one endangered), and nine avifauna were protected. The correlation of avifauna diversity to biotic and abiotic factors was positive. These results provide essential data for further conservation management, emphasizing the protection of the forest area, tourists, waterfalls, and slopes of Mount Kawi. Additionally, studying spatial and temporal distribution is crucial for future avifauna species re-inventory efforts.
Exploring Microbial Diversity in Green Honey from Pulau Banggi Sabah: A Preliminary Study: Microbial Diversity in Green Honey from Pulau Banggi Sabah Rajindran, Nanthini; Ab Wahabb, Roswanira; Huda , Nurul; Adekilekun Oyewusi, Habeebat; Wayan Gunam, Ida Bagus; Mohd Shariff , Amir Husni; Izzah Ismail, Norjihada; Huyop, Fahrul
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.02

Abstract

The microbiological composition of honey can include microorganisms that are beneficial or harmful to human health. Therefore, it is essential to investigate the microbiological quality of different honey types available in the market. However, there is limited information available on the analysis, isolation, and characterization of honey-associated microbes, especially for green honey from Banggi Island. Green honey is sourced from underground areas within the island's forest. This study aimed to assess the microbiological quality of raw (freshly collected) and processed green honey by examining the presence of bacteria, yeast, molds, and pathogens. The results revealed that raw green honey had a slightly higher total plate count (770 ± 0.03 cfu/g) compared to processed green honey (640 ± 0.02 cfu/g). Both raw and processed green honey contained Lactobacillus spp. with counts of 350 ± 0.02 cfu/g and 160 ± 0.02 cfu/g, respectively. Bacillus count was higher in raw green honey (110 ± 0.01 cfu/g) compared to processed green honey (5 ± 0.01 cfu/g). Molds were only detected in raw green honey, while osmophilic yeast counts were higher in raw green honey (16000 ± 0.03 cfu/g) compared to processed green honey (120 ± 0.02 cfu/g). Mesophilic bacteria, thermophilic bacteria, coliforms, E. coli, and Staphylococcus aureus were not detected in either raw or processed green honey. Furthermore, green honey was free from pathogenic bacteria such as Salmonella spp., Listeria spp., and Shigella spp. Bacteria isolated from green honey included Lysinibacillus macrolides, Lysinibacillus boronitolerans, Paenibacillus cineris, Paenibacillus favisporus, and Bacillus oleronius, none of which were pathogenic. This study identified important microorganisms present in green honey, which have the potential to provide beneficial effects without posing any harm to human health.
An Overview of Traditional Medicinal Plants Used in Treating Hepatocellular Carcinoma (HCC) with Emphasis on Mechanisms of Action: Traditional Medicinal Treatment of Hepatocellular Carcinoma (HCC) Adekilekun, Habeebulahi Ajibola; Oyewusi, Habeebat A.; Odoma, Saidi; Kirimuhuzya, Claude
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.20

Abstract

Cancer is an incurable fatal disease wreaking havoc on the population of countries either developed or developing. Hepatocellular carcinoma (HCC) is the fifth most frequent type of cancer having a poor prognosis. Globally, HCC management and treatment intervention using medicinal plants from generation to generation is rapidly increasing and remains an area of promising research. This review provided insight into medicinal plants' ethnomedicinal use, photochemistry and mechanisms of action on HCC exhibiting anti-cancer potentials subjected to scientific investigations. Furthermore, clinical, toxicity studies and detailed mechanisms of action would provide an understanding of the development of lead compounds in drug discovery and development for HCC
Alterations in Blaood Profiles and Spleen Tissue Following Purple Sweet Pota-to Anthocyanin Extract Treatment in Restraint-Stressed Mice: Blood Profiles and Spleen Tissue Following Purple Sweet Potato Anthocyanin Extract Treatment Kurnianingsih, Nia; Artamevia, Dian; Hasanah, Dian; Harbiyanti, Novita Titis; Ratnawati, Retty
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.19

Abstract

Stress is associated with broad changes in blood and immune cell dysfunction, which are correlated with the spleen as a secondary lymphoid organ. Anthocyanin, a plant bioactive compound from purple sweet potatoes, has previously been reported to have a beneficial effect on stress response behavior. However, the limited studies evaluated anthocyanin's effect on blood profiles and spleen tissue under stress. Therefore, this study aimed to explore the effect of total anthocyanin extract (ANC) from purple sweet potatoes (PSP) on the blood profiles and spleen tissue of restraint stressed mice. A total of 20 male adult BALB/c mice were divided into control (CTRL), stress (STR), stress + ANC 10 mg/kgBW (STR+ANC10), stress + ANC 20 mg/kgBW (STR+ANC20), and stress + ANC 40 mg/kgBW (STR+ANC10). Restraint stress was applied two hours/day for 14 days. An automatic blood analyzer was used for blood profiling. Immunoblotting of caspase-3 and hematoxylin-eosin-stained histological slides were observed for spleen tissue analysis. Combination of STR and ANC treatment reduced the erythrocyte count, hemoglobin level, and leucocyte count. The ANC-treated groups had a higher percentage of lymphocytes and a lower percentage of granulocytes. The expression of caspase-3 declined among ANC-treated groups. Spleen extramedullary erythropoiesis, vacuolization, and white pulp involution were demonstrated in stress and ANC-treated animals. In summary, restraint stress modified the blood cell profiles and spleen tissue in the mice model. Further research is necessary to evaluate the mechanism of ANC during stress on blood cells and splenic tissue repair enhancement.
The effect of Phyllanthus niruri and Catharanthus roseus on Macrophage Polarization in Breast Cancer Mice Model: The Effect of P. niruri and C. roseus in Breast Cancer Mice Model Sakti, Sefihara Paramitha; Sari, Fikriya Novita; Rachmawati, Farida; Widyarti, Sri; Rahayu, Sri; Soewondo, Aris; Jatmiko, Yoga Dwi; Rifa'i, Muhaimin
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.03

Abstract

Cancer death cases have increased yearly, and there are estimated to be 21.6 million cancer cases in 2030. Studies of herbal compounds for cancer treatment alternatives are essential because cancer treatment is relatively expensive and has adverse effects. Phyllanthus niruri (Pn) and Catharanthus roseus (Cr) are plants that are known as herbal medicines. Combining the two plants is expected to prevent and enhance the immune system in breast cancer cases. This study aims to analyze the anti-cancer and immunomodulatory effects of P. niruri and C. roseus extract (PCE) in modulating macrophage polarization in breast cancer mice. Experimental animals are divided into six groups and there is healthy control (normal mice), cancer (DMBA-induced mice), cancer mice with cisplatin administration, cancer mice with PCE administration with three different doses, including dose 1 (500 mg/kg Pn + 15 mg/kg Cr), dose 2 (1000 mg/kg Pn + 75 mg/kg Cr), and dose 3 (2000 mg/kg Pn + 375 mg/kg Cr). The mice were injected with DMBA once a week for six weeks to induce cancer in mice. The breast cancer mice model was administered with PCE orally for 14 days. The expression of CD11b+IL-10+ and CD11b+IFN-γ+ demonstrated macrophage polarization. The results showed that breast cancer induction using DMBA increased the level of IL-10 and decreased the level of IFN-γ significantly compared to the normal group (p < 0.05). In specific doses, administration of PCE could reduce IL-10 levels and increase the level of IFN-γ significantly (p < 0.05). PCE can modulate the polarization of macrophages by suppressing the M2-like macrophage and increasing the M1-like macrophage. The ability of PCE to modulate macrophage polarization indicates that the combination of P. niruri and C. roseus has activity as an anti-cancer.
The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Population: The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Atmaja, Sarah; Ellsya Angeline Rawar; Ani Kristiyani; Dwi Aris Agung Nugrahaningsih; Ahmad Hamim Sadewa; Christine Patramurti
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.06

Abstract

Polymorphisms of CYP3A4*1G and CYP3A5*3 affect the pharmacokinetic profile of various drugs, e.g., fentanyl, tacrolimus, diltiazem, simvastatin. Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) is a simple and economical method for SNP determination. The polymorphisms in the CYP3A4*1G and CYP3A5*3 genes have not yet been examined using this method in Javanese Indonesian. Our aim was to determine the frequency of polymorphisms in the CYP3A4*1G and CYP3A5*3 genes in Indonesian Javanese using the ARMS-PCR method. Eighty-six patients at the Kalasan Community Health Centre in Yogyakarta, Indonesia, were chosen based on the inclusion criteria, which is Javanese ancestry. They gave their informed consent to blood collection by completing a form. Genetic variants were detected using Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR). The chi-square test was used to determine genotype deviations from Hardy-Weinberg equilibrium, with a significant threshold of 0.05. For homozygous wild types, CYP3A4 *1/*1 dominated overall among study participants (73.35%), whereas for CYP3A5*3/*3, homozygous mutants were more prevalent (83.72%). Hardy-Weinberg equilibrium is consistent with genotype frequencies (p > 0.005). One participant carried a homozygous mutation for both CYP3A4*1G and CYP3A5*3, while the other 49 subjects were heterozygous for CYP3A4*1G and homozygous mutant for CYP3A5*3, which is the highest number of SNP combinations. The findings of the current investigation demonstrate that the population has the highest proportion of homozygous CYP3A4*1G wild-types (CYP3A4*1/*1) and homozygous mutants for CYP3A5*3 (CYP3A5*3/*3)

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