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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
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Produksi Glukosa dengan Substrat Selulosa Kasar Brangkasan Jagung Menggunakan Enzim Selulase dari Isolat B2S8 Rendy Sinaga; Ida Bagus Wayan Gunam; Nyoman Semadi Antara
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p14

Abstract

The aim of this study is to determine the crude cellulose corn stover substrate concentration and saccharification time to produce high glucose. Cellulose contained in the corn stover has potential converted into glucose by enzymatic hydrolysis using cellulase enzymes. Glucose production in different substrate concentrations and saccharification times uses a factorial randomized block design (RBD) consisting of two factors. The first factor is the substrate concentration which consists of 4 levels, namely 2%, 3%, 4%, and 5% (w/v). The second factor is the saccharification time which consists of 4 levels, namely 24 hours, 48 hours, 72 hours, and 96 hours. The observed variables include glucose level, cellulose residue, pH, and total dissolved solid. The results showed that 5% substrate concentration and 96 hours saccharification time was the best treatment for producing the maximum glucose level was 0,3003 mg/mL, the minimum cellulose residue after saccharification was 17,75%, pH after saccharification was 6,1, and the highest total dissolved solid after saccharification was 2,7°Brix.
Daily Activity of Southern Cassowary (Casuarius casuarius Linnaeus, 1758) in Bali Zoo, Gianyar Alia Ambara Putri Purbaya; Luh Putu Eswaryanti Kusuma Yuni; Fransiscus Xaverius Sudaryanto
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p05

Abstract

Burung Kasuari Gelambir Ganda (Casuarius casuarius Linnaeus, 1758) merupakan satwa endemik yang keberadaannya memiliki pengaruh besar dalam ekologi hutan di Papua. Sebagai frugivora, burung ini berperan untukmenyebarkan biji pohon buah-buahan yang merupakan 90% dari spesies pohon di hutan Papua. Namun populasi burung ini di habitat alaminya mengalami penurunan akibat konversi hutan seiring perkembangan penduduk, dan diperparah oleh perburuan oleh masyarakat setempat. Konservasi ex-situ dilakukan dengan tujuan untuk mengembangbiakkan satwa di luar habitat aslinya, sehingga penelitian aktivitas harian spesies burung Kasuari Gelambir Ganda perlu dilaksanakan. Penelitian dilakukan dari tanggal 10 Januari sampai 21 Februari 2022 terhadap lima individu burung Kasuari Gelambir Ganda di Bali Zoo. Metode yang digunakan yaitu focal animal sampling dengan instantaneous recording selama 30 menitdengan interval waktu satu menit. Data yang diperoleh ditabulasi dan dianalisis menggunakan Microsoft Excel, kemudian dijabarkan secara deskriptif. Aktivitas yang paling banyak dilakukan oleh burung Kasuari Gelambir Ganda di Bali Zoo adalah istirahat (35,69%), dilanjutkan dengan aktivitas bergerak (19,69%), mencari makan (18,58%), menelisik bulu (16,74%), waspada (6,69%), vokalisasi (1,19%), defekasi (0,48%), dan urinasi (0,14%). ini menunjukan bahwa burung Kasuari Gelambir Ganda dapat merefleksikan aktivitas hariannya secara alami selama berada di lembaga konservasi Bali Zoo yang mengindikasikan kesejahteraan satwa terpenuhi. Kata Kunci : Aktivitas Harian, Bali Zoo, Kasuari Gelambir Ganda, Konservasi
Efek Ekstrak Daun Bayam Brasil (Alternanthera sissoo hort) Terhadap Jumlah Limfosit dan Indeks Organ Timus dan Limpa Mencit Jantan Priskila Melinia Wuni; Kukuh Madyaningrana; Vinsa Cantya Prakasita
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p19

Abstract

Utilization of flora biodiversity is very potential to be explored as a source of medicine. Their phytochemicals content could be used as immunomodulator to help body's immunity fight pathogen. Brazilian spinach has the potential to be developed as an herbal-based immunostimulant because of its nutritional value and phytochemical compounds. This study aimed to study the effect of Brazilian spinach leaf extract on the immune response of male mice as shown by lymphocyte count and spleen and thymus index values. Extract of Brazilian spinach leaf was obtained by maceration method using 96% ethanol as solvent. Phytochemical were detected using qualitative biochemical methods and GC-MS. The in vivo test was carried out by giving 6 treatments to male mice orally in 7 days, namely aquades treatment, negative control (Na-CMC-aquadest), positive control (commercial immunostimulants), and three doses of Brazilian spinach leaf extract, namely 0.1563 mg/g. BB; 0.312 mg/g BW), and 0.468 mg/g BW. The number of lymphocytes was prepared and counted by blood smear, as well as the calculation of the spleen and thymus index based on fresh weight calculation. Qualitative biochemical assays showed identified flavonoids, alkaloids, saponins, tannins and steroids in Brazilian spinach leaf extract, meanwhile GC-MS assays showed 3 dominant compounds, namely neophytadiene, phytol and -Tocopheryl acetate. The administration of Brazilian spinach leaf extract with a dose of 0.1563 mg/g BW was the most effective in increasing lymphocytes count of tested animals. The thymus and spleen index values ??correlated with the increased number of lymphocytes in the administration of Brazilian spinach leaf extract. These results indicate the basic potential of using Brazilian spinach leaves as an immunostimulant.
Isolasi Dan Karakterisasi Bakteri Heterotrofik Pada Kawasan Perairan Pantai Indah Kalangan, Tapanuli Tengah Wanda fadilah; Rasyida .; Ulfayani Mayasari
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p10

Abstract

This research aims to determine the presence of heterotrophic bacteria in the coastal waters of Indah Kalangan, Central Tapanuli and what genus of heterotrophic bacteria were found in the coastal waters of Indah Kalangan, Central Tapanuli. The working procedure in this research is the characterization of bacterial morphology, gram staining and also characterization based on biochemical tests. The descriptive method will be used in the data analysis process using Bergey's Manual of Determinative Bacteriology 8th Edition, Bergey's Manual Of Systematic Bacteriology 2nd Edition and journals. The results of this study obtained 16 isolates of heterotrophic bacteria, of which 13 bacterial isolates belonged to the Bacillus genus and 3 bacterial isolates belonged to the Vibrio genus. The results showed that the coastal waters of Indah Kalangan contained heterotrophic bacteria with the genus Bacillus and the genus Vibrio. Keywords: Heterotrophic Bacteria, Bacillus, Vibrio, Indah Kalangan Beach
Kuantifikasi Protein HAS2, PTX3, BCL2 dan BAX dalam Sel Kumulus Oosit Matur (MII) dan Imatur (MI) Pada Pasien Bayi Tabung Ni Putu Sri Risa Dewi; Ngurah Intan Wiratmini; Jaqueline Sudiman
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p01

Abstract

Oocyte developmental competence is one of the determining factors that in?uence the outcomes of an IVF cycle regarding the ability of a female gamete to reach maturation and be fertilized. A non-invasive approach using proteomic analysis of oocyte cumulus cells (CCs) can be used as a method to predict oocyte competence and viability that could potentially function as molecular predictors for IVF program prognosis. Our study was aimed at quantifying protein markers (HAS2, PTX3, BCL2 and BAX) that are expressed in human CCs between mature (MII) and immature (MI) oocytes. This research is an analytic observational study. Twelve samples of CCs from mature (MII) and immature (MI) oocytes were collected after ovum pick up from patients undergoing ICSI. CCs samples were isolated using an ultrasonicator and protein expressions were quantified using ELISA method. The Mann-Whitney test was used to compare the protein expressions from CCs between mature and immature oocytes. The results showed HAS2, PTX3 and BCL2 proteins expressions was increase in CCs from mature oocytes but had no significant effect compared to CCs from immature oocytes, however, BAX protein expression was significantly higher (P<0.05) in CCs from immature oocytes which CCs from mature oocytes. This present study shows that BAX proteins could be used as markers for oocyte quality and maturation. Key words: ELISA, maturation, proteins marker, cumulus cells (CCs)
Analyze Of Cloves (Syzygium aromaticum L.) Leaves Essential Oil Grow From Different Elevations Range Luh Gede Artha Saridewi Wijaya; Ni Luh Arpiwi; Ida Ayu Astarini
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p15

Abstract

ABSTRACT A research was carried out to determine the yield, content of constituent compounds, and analysis of the quality of clove leaf essential oil (Syzygium aromaticum L.) at different altitudes. The study used dry clove leaves obtained from Buleleng Regency, Bali Province with an altitude of 300 meters above sea level (masl), 600 masl, and 900 masl. Extraction of essential oils was carried out by steam distillation. The essential oil obtained was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS) and the quality was tested using the quality requirements of SNI 06-2387-2006. Parameters observed were yield, identification of constituent compounds, analysis of essential oil quality including color, odor, specific gravity, and refractive index, solubility in alcohol, optical rotation, total eugenol and caryophyllene. The results showed that clove leaf essential oil in the lowlands (300 masl) had a higher yield of 2.05% and more types of active compounds than in the optimal altitude (600 masl) which was 1.45% and the highlands (900 masl) was 1.85%. The main compounds that make up clove leaf essential oil were eugenol, caryophyllene, and humulene. Other compounds were longifoline, nootkatone, naphthalene, citronellal, and cyclohexene. The clove leaf essential oil from the three heights met the quality requirements of SNI 06-2387-2006. Keywords: Essential oil, cloves, eugenol, GC-MS, secondary metabolites
Extraction and Composition of Nutmeg’s Essential Oils (Myristica fragrans Houtt.) Based on Seed Skin Color Florian Mayesti Prima Remba Makin; Welsiliana .; Angreni Beaktris Liunokas
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p06

Abstract

Pala (Myristica fragrans Houtt.) merupakan tumbuhan asli Indonesia. Salah satu produk pala yang menjadi komoditas dagang dan diekspor adalah biji. Biji pala atau yang biasa disebut nutmeg banyak diminati karena mengandung banyak senyawa aromatik dalam bentuk minyak asiri. Penelitian ini bertujuan mengekstraksi dan mengetahui komposisi minyak asiri biji berdasarkan perbedaan warna kulit biji. Sampel segar diekstrak dan dianalisis menggunakan GC-MS. Hasil menunjukan dua belas senyawa diidentifikasi pada B1 dan sebelas senyawa biji B2 yang didominasi monoterpena masing-masing 85,76% dan 80,56%; sisanya senyawa fenol-eter aromatik. Monoterpena dominan biji B1 dan B2, di antaranya adalah sabinena (48,57%; 46,38%), ?-pinena (13,86%; 12,45%), dan ?-pinena (13,36%; 12,36%). Senyawa fenol-eter aromatik seperti miristisin (11,42%) dan eugenol (0,62%) pada biji B1 dan miristisin (16,50%) dan metoksieugenol (0,92%) pada biji B2. Minyak asiri biji pala didominasi oleh senyawa monoterpena dan sedikit senyawa fenol-eter aromatik. Kata kunci: biji pala, fenol-eter aromatik, minyak asiri, monoterpena
Pemberian Ekstrak Etanol Biji Klabet (Trigonella foenum-graecum L.) Menurunkan Kualitas Spermatozoa Mencit (Mus musculus) Dewasa Muda Putu Susilawati; Bagus Komang Satriyasa; I Gusti Ayu Widianti
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p11

Abstract

This study aimed to determine the activity of the ethanol extract of Fenugreek Seed (Trigonella foenum-graecum Linn.) to reduce the sperm quality of young adult mice (Mus musculus) as a natural male contraceptive. This research is an experimental study using a post-test only control group design. The sample of this study consisted of 36 male Swiss Webster (Mus musculus) mice aged 2-3 months with a body weight of 20-30 grams which were divided into two groups randomly. The control group (P0) was given 0.5 ml of CMC Na 0.3% and the treatment group (P1) was given the ethanol extract of Fenugreek Seed (Trigonella foenum-graecum Linn.) 0.4 mg/gram BW as much as 0.5 ml for 36 day orally. The results showed that the administration of ethanol extract of Fenugreek Seed (Trigonella foenum-graecum Linn.) could reduce the quality (concentration, motility, viability, and morphology) of spermatozoa (P?0.05), so that Fenugreek Seed (Trigonella foenum-graecum Linn.) has potential as a natural male contraceptive.
Potensi Ekstrak Etanol Daun Kelor (Moringa Oleifera) Terhadap Kualitas Spermatozoa dan Hormon Testosteron Tikus Putih Jantan yang Diinduksi Meloxicam Putu Mila Ayustina; Ni Made Rai Suarni; Ni Gusti Ayu Manik Ermayanti
Metamorfosa: Journal of Biological Sciences Vol 9 No 2 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i02.p03

Abstract

Meloxicam adalah obat anti inflamasi nonsteroid (NSAIDs) yang diberikan pada manusia dan hewan untuk mengobati peradangan paling banyak diresepkan. Penggunaan meloxicam yang tidak sesuai dengan ketentuan dosis dan dalam jangka panjang akan menimbulkan efek toksisitas dari meloxicam. Meloxicam dapat memicu stress oksidatif dan memiliki mekanisme kerja menghambat sintesis prostaglandin yang dapat mempengaruhi proses regulasi hormonal dan fungsi reproduksi jantan. Tujuan penelitian ini adalah untuk menganalisis potensi ekstrak etanol daun kelor (Moringa oleifera) terhadap kualitas spermatozoa dan kadar hormon testosteron jantan tikus putih (Rattus norvegicus) jantan yang diinduksi Meloxicam. Penelitian ini menggunakan rancangan jenis (RAL) Rancangan Acak Lengkap terdiri dari tiga perlakuan dan dua kontrol dengan 6 ulangan. Pemberian perlakuan dilakukan selama 35 hari. Meloxicam diberikan dosis 8,4 mg/kg BB dan ekstrak etanol daun kelor dosis 200 mg/kgBB/hari (P1), 400 mg/kgBB/hari (P2), dan 600 mg/kgBB/hari (P3). Hasil penelitian ini menunjukkan bahwa ekstrak etanol daun kelor dosis 400 mg/kgBB dan 600 mg/kgBB berpotensi meningkatkan berat organ reproduksi, motilitas, viabilitas, jumlah spermatozoa, dan meningkatkan kadar hormon testosteron secara signifikan (P<0,05).
Isolasi Bakteri Dari Rumput Laut Eucheuma spinosum dan Skrining Aktivitas Antibakteri Terhadap Beberapa Bakteri Gram Positif dan Negatif I Gusti Agung Ayu Sucitra Ekaryani; Anak Agung Gede Indraningrat; Ni Made Ayu Suardani Singapurwa; I Wayan Sudiarta; Anak Agung Made Semariyani; I Putu Candra
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p01

Abstract

Rumput laut merupakan salah satu biota laut yang berinteraksi kuat dengan mikroorganisme khususnya dengan kelompok bakteri. Bakteri yang berasosiasi dengan rumput laut dapat memproduksi metabolit sekunder untuk mendukung pertumbuhan dan melindungi inangnya. Penelitian ini bertujuan untuk mengisolasi bakteri yang berasosiasi dengan rumput laut Eucheuma spinosum dan menskrining aktivitas antibakteri dari isolat bakteri yang diperoleh. Hasil isolasi bakteri dari E. spinosum diperoleh 32 isolat dengan sebaran 14 isolat dari media umum dan 18 isolat dari media khusus. Pengamatan morfologi dari 32 isolat menunjukkan bahwa tujuh isolat bakteri tergolong Gram positif dan 25 isolat bakteri Gram negatif. Sedangkan 25 isolat menunjukkan katalase positif dan tujuh isolat katalase negatif. Hasil pre-skrining antibakteri terhadap bakteri Gram positif (Staphylococcus aureus atcc 25923 dan Streptococcus mutans fncc 0405) dan bakteri Gram negatif (Escherichia coli atcc 25922 dan Klebsiella pneumoniae atcc 700603) menunjukkan 22 isolat memiliki aktivitas antibakteri. Isolat PCAR1 dan SMPR9 menunjukkan aktivitas antibakteri tertinggi terhadap Streptococcus mutans berturut-turut sebesar 9,21 mm dan 7,89 mm. Hasil penelitian ini secara umum memberikan gambaran potensi isolat yang berasosiasi dengan E. spinosum sebagai penghasil senyawa antibakteri.

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