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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 21 Documents
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Search results for , issue "Vol 18 No 1 (2017)" : 21 Documents clear
Seroprevalensi Sistiserkosis pada Babi di Papua (SEROPREVALENCE OF PIG CYSTICERCOSIS IN PAPUA REGION) Ida Bagus Ngurah Swacita; I Ketut Suada; Ketut Budiasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; I Nyoman Polos; I Made Damriyasa
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.971 KB) | DOI: 10.19087/jveteriner.2017.18.1.18

Abstract

Pig cysticercosis is an infection caused by the larval stage of pork tapeworm and Papua is one of the largest endemic areas of cysticercosis in Indonesia. This survey aim was to determine the seroprevalence of pig cystisercosis in Papua. A total of 311 pig serum samples collected from six regencies in Papua were examined using Enzyme Linked Immunosorbent Assay (ELISA). The result of the survey showed that the average seroprevalence of pig cysticercosis in Papua was 23.5% (73/311), where the highest seroprevalence was found in the regency of Jayawijaya was 42.6% (43/101), Biak 22.5% (9/40), Nabire 20.6% (7/34), Mimika 17% (8/47), Jayapura 13.5% (5/37), and Merauke 1.9% (1/52). It can be concluded that the seroprevalence of pig cysticercosis in Papua is still high, therefore, it is necessary to do more intensive programs to prevent and control this disease. ABSTRAK Sistiserkosis pada babi adalah infeksi yang disebabkan oleh stadium larva cacing pita, dan Papua merupakan salah satu daerah endemis sistiserkosis di Indonesia. Tujuan penelitian ini adalah untuk menentukan seroprevalensi sistiserkosis pada babi di Papua. Sebanyak 311 sampel serum babi yang dikumpulkan dari enam kabupaten di Papua diuji dengan Enzyme Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan bahwa rataan seroprevalensi sistiserkosis pada babi di Papua sebesar 23,5% (73/311), dan seroprevalensi terbesar ditemukan di Kabupaten Jayawijaya 42,6% (43/101), Biak 22,5% (9/40), Nabire 20,6% (7/34), Mimika 17% (8/47), Jayapura 13,5% (5/37), dan Merauke 1,9% (1/52). Dapat disimpulkan bahwa seroprevalensi sistiserkosis pada babi di Papua masih tinggi, sehingga diperlukan program yang lebih intensif untuk mencegah dan mengontrol penyakit ini.
Pemberian Buah Merah (Pandanus conoideus Lam) sebelum Dipapar Timah Hitam Menekan Ekspresi Caspase-8 dan Jumlah Sel Hofbauer Mencit (Mus musculus) Bunting (THE PROVISION OF RED FRUIT (Pandanus conoideus Lam) BEFORE EXPOSED BY LEAD DECREASE EXPRESSION OF C Ika Wahyuni; Widjiati Widjiati; Sri Pantja Madyawati; Fedik Abdul Rantam
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.904 KB) | DOI: 10.19087/jveteriner.2017.18.1.128

Abstract

The research aims to observed the provision of red fruit in placenta pregnat mice before exposed by lead. The observed case are expression of caspase-8 and number of hofbauer cells. Red fruit was expected to decrease expression of caspase-8 and number of hofbauer cells. The study design used was complete randomized design. Each treatment utilized four pregnant mice as negative control group (K-), for this group given distilled water orally during gestation 6th-15th. Positive control group (K+) given lead (0.011mg/20 g BW in 1 mL distilled water) orally during gestation 6th-15th. The treatment group namely P1, P2, and P3 treated by red fruit with different doses i.e., 8.29 mg/20 g BW, 23.98 mg/20 g BW, and 25.68 mg/20 g BW, respectively, during gestation 6th-15th, then one hour later exposed with lead. The obtained data were analyzed by Kruskal-Wallis and Mann Whitney test for calculate caspase-8 expression, ANOVA and Duncan for calculate the number of hofbauer cells. The result indicated that treatment groups which provided by red fruit of 0.8 mL/20 g BW and 0.9 mL/20 g BW showed decline expression of caspase-8 and number of hofbauer cells compare control group without admission of red fruit antioxidant and P1. Inconclusion red fruit can decrease expression of caspase-8 and number of hofbauer cell that means decrease apoptosis. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian buah merah pada plasenta mencit bunting sebelum dipapar timah hitam. Variabel yang diamati adalah ekspresi caspase-8 dan jumlah sel hofbauer. Buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer. Penelitian ini menggunakan rancangan acak lengkap (RAL). Setiap kelompok perlakuan menggunakan empat mencit bunting sebagai kelompok kontrol negatif (K-), untuk kelompok ini diberi aquades pada kebuntingan hari ke-6 sampai 15. Kelompok kontrol positif (K+) diberi timah hitam dosis 0,011 mg/20 g BB dalam 1 mL aquades selama kebuntingan hari ke-6 sampai 15. Kelompok perlakuan (P1, P2, dan P3) diberi minyak buah merah dengan dosis yang berbeda yaitu 8,29 mg/20 g BB, 23,98 mg/20 g BB, dan 25,68 mg/20 g BB. Data dianalisis dengan Kruskall-Wallis dan Mann Whitney untuk menghitung ekspresi caspase-8, sidik ragam dan uji Duncan digunakan untuk menghitung jumlah sel hofbauer. Hasil penelitian menunjukkan kelompok perlakuan yang diberi buah merah 23,98 mg/20 g BB dan 25,68 mg/20 g BB dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer dibandingkan dengan kelompok kontrol positif dan P1. Simpulan penelitian ini adalah bahwa minyak buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer yang berarti menekan terjadinya apoptosis.
Kompetensi Maturasi dan Fertilisasi Oosit Domba Prapubertas Secara In Vitro (DEVELOPMENTAL COMPETENCE OF MATURATION AND FERTILIZATION PREPUBERTAL SHEEP OOCYTES IN VITRO) Anita Hafid; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.613 KB) | DOI: 10.19087/jveteriner.2017.18.1.51

Abstract

The objective of this study was to evaluate the maturation and fertilization ability of oocytes frompre-pubertal sheep ovary in vitro. Prepubertal ovary were collected based on the absence of corpus luteum or absence of corpus albicans in both of the ovaries. Oocytes were collected by slicing methode. Only the oocytes which categories of homogeneous cytoplasm and compact cumulus cells were used and it was matured for 24 hours in CO2 incubator with temperature 39oC. Oocytes fertilized in vitro used post thawed spermatozoa with concentration 5x106 and incubated for 12-14 hours. Oocytes were evaluated on number of oocytes reached MII and number of PN formation. Result of the experiment revealed that there was no significant difference in the percentage of MII oocytes after in vitro maturation (89% vs 90,7%, P>0,05) between prepubertal sheep and pubertal sheep. Meanwhile, the fertilization rate was significantly lower (P>0,05) in prepubertal sheep oocytes compared to pubertal sheep oocytes (60% vs 77,7%). The incidence of polispermic fertilization was higher in prepubertal sheep oocytes than pubertal sheep oocytes (21,8% vs7,4%, P>0,05). In conclusion, prepubertal sheep oocytes and pubertal sheep oocytes have similar in vitromaturation ability, even though the ability to be fertilized of the prepubertal sheep oocytes is lower than the pubertal sheep. ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan maturasi dan fertilisasi oosit dari ovarium domba prapubertas secara in vitro. Ovarium domba prapubertas dikoleksi berdasarkan ketidakhadiran korpus luteum atau ketidakhadiran korpus albicans pada kedua ovarium. Oosit dikoleksi dengan metode slicing. Hanya oosit dengan sitoplasma yang homogen dan sel kumulus yang kompak yang dipakai dan dimaturasi selama 24 jam dalam inkubator CO2 dengan suhu 39oC. Oosit difertilisasi secara in vitro menggunakan semen beku dengan konsentrasi 5x106 spermatozoa/mL dan diinkubasi selama 12-14 jam. Pengamatan dilakukan terhadap kemampuan oosit mencapai tahap metafase II (MII) dan pembentukan pronukleus (PN). Hasil penelitian menunjukkan status inti oosit pada tahap MII tidak berbeda antara oosit domba prapubertas dan oosit domba pubertas (89% vs 90,7%, P>0,05) setelah dimaturasi secara in vitro. Sementara tingkat fertilisasi oosit domba prapubertas lebih rendah (P>0,05) dibandingkan dengan oosit domba pubertas (60,0% vs 77,7%). Kejadian polispermi pada oosit domba prapubertas cenderung lebih tinggi dibandingkan dengan oosit domba pubertas (21,8% vs 7,4%, P>0,05). Dapat disimpulkan bahwa oosit domba prapubertas memiliki kemampuan maturasi yang sama dengan oosit domba pubertas namun memiliki kemampuan fertilisasi yang lebih rendah.
Daun Binahong (Andredera cordifolia Steenis) Sebagai Alternatif Insektisida Terhadap Miasis yang Disebabkan Lalat Chrysomya bezziana (ANREDERA CORDIFOLIA STEENIS (BINAHONG) LEAF AS AN ALTERNATIVE INSECTICIDE AGAINST CHRYSOMYA BEZZIANA CAUSED MYIASIS) Ietje Wientarsih; Aulia Andi Mustika; April Hari Wardhana; Dodi Darmakusumah; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.219 KB) | DOI: 10.19087/jveteriner.2017.18.1.121

Abstract

Larvae Chrysomya bezziana caused myiasis in most livestock in Indonesia. Drugs of choice for treating myasis are synthetic insecticides. Unfortunately the insecticides has negative effect on animal product, killed insect non target and insect resistenst. The research was conducted on the activity of ethanol extract of binahong leaves against various stages of C. bezziana larvae (L1, L2, and L3). Five treatments group tested were: group without any treatment (negative control), groups treated with ethanol extracts of binahong leaves 0.5%, 1%, and 2%, respectively, positive control were given coumaphos. The treatments were applied with five replications. Number of living larvae and pupae, pupae weight and number of imago were observed. The results demonstrated that 0.5% of the extract effective consentration which was able to kill the larvae (L1 and L2). Finally 2% of the extract was the most effective concentration which was able to kill larvae (L3) and decrease the pupae weight. Ethanol extract of binahong leaves was significantly able to reduce the growth of C. bezziana larvae due to contact and digestive effect of the active compounds contained in Anredera cordifolia leaf. ABSTRAK Miasis merupakan infestasi larva lalat yang terdapat pada jaringan hidup. Penyakit ini umum menyerang hewan maupun manusia. Obat yang digunakan sebagai pengendalian kasus miasis di lapangan sampai saat ini sangat terbatas dan menggunakan insektisida sintetik. Penelitian ini bertujuan melihat aktivitas ekstrak etanol daun binahong terhadap larva lalat Chrysomya bezziana pada stadium L1, L2, dan L3. Penelitian dibagi menjadi lima kelompok perlakuan dengan lima ulangan. Kelompok perlakuan terdiri dari kontrol negatif (tanpa pemberian obat), kelompok perlakuan yang diberikan ekstrak binahong dengan konsentrasi berturut-turut 0,5%, 1%, 2%, dan kelompok yang diberikan coumaphos (kontrol positif). Peubah yang diamati adalah jumlah larva yang hidup dan berkembang menjadi pupa, bobot pupa, dan daya tetas pupa menjadi imago. Hasil penelitian menunjukkan bahwa ekstrak etanol daun binahong pada konsentrasi 0,5% mempunyai efikasi sebagai larvasidal terhadap L1 dan L2 serta pada konsentrasi 2% merupakan konsentrasi terbaik sebagai larvasidal L3 larva C. bezziana. Ekstrak etanol daun binahong memiliki kemampuan menghambat larva lalat C. bezziana akibat dari efek cerna dan efek kontak bahan aktif yang terkandung di dalam daun binahong.
Blood Profile of Domestic Cat (Felix catus) During Skin Graft Recovery with Different Period (PROFIL DARAH KUCING LOKAL SELAMA KESEMBUHAN AUTO-SKIN GRAFT DENGAN WAKTU YANG BERBEDA) Erwin Erwin; Gunanti Gunanti; Ekowati Handharyani; Deni Noviana
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.134 KB) | DOI: 10.19087/jveteriner.2017.18.1.31

Abstract

The aim of this research is to observe blood profile of domestic cats which includes red blood cell, white blood cell, haemoglobin, haematocrit, and trombocyte during skin graft recovery period via autograft. A total of nine male domestic cats (Felis catus) aged 1-2 years weighting 3-4 kg were separated into three treatment groups. Hair shaving and disinfectant application were done on lateral area of front leg, and then 2x2 cm incision was made. The wound was wrapped by sterile gauze dampened by poviderm iodine and left for different period of days per treatment group; where Group I (two days), Group II (four days), and Group III (six days). Transplantation was done by taking the skin on thorax area and placing it on the recipient after first cleaning subcutaneous tissue from the skin surface of donor’s wound and the base of recipient’s wound. Blood sample was taken from vena cephalica antebrachii anterior on day 0 before skin graft, on day three, six, nine, 12, and 18 after skin graft. Based on the result, significant difference waso bserved from red blood cell count, white blood cell count, haemoglobin count, and hematocrit, whereas trombocyte count did not show any significant difference between treatment groups. The cats on Group II and Group III were systemic good condition compared to Group I. ABSTRAK Penelitian ini bertujuan melihat profil darah kucing lokal, meliputi sel darah merah, sel darah putih, hemoglobin, hematokrit, dan trombosit selama kesembuhan tranplantasi kulit secara autograft. Sebanyak sembilan ekor kucing lokal (Felix catus) jantan umur 1-2 tahun dengan bobot badan 3-4 kg dibagi menjadi tiga kelompok perlakuan. Pada kucing tersebut dilakukan pencukuran rambut dan desinfeksi pada area kaki depan sisi lateral, selanjutnya dibuat luka sayat/incisi 2x2 cm. Luka dibalut dengan kasa steril yang dibasahi povidone iodine dan dibiarkan selama beberapa hari. Kucing dengan sayatan yang dibiarkan tersebut dikelompokkan menjadi kelompok I (dua hari), kelompok II (empat hari), dan kelompok III (enam hari). Transplantasi dilakukan dengan mengambil kulit dari area thorak dan ditempatkan pada resipien dengan terlebih dahulu permukaan luka kulit donor dan dasar luka resipien dibersihkan dari jaringan subkutis. Pengambilan sampel darah dilakukan melalui vena cephalica antebrachii anterior pada hari ke-0, 3, 6, 9, 12, dan 18. Berdasarkan hasil penelitian terjadi perbedaan yang signifikan (P<0,05) terhadap jumlah sel darah merah, sel darah putih, hemoglobin, dan hematokrit, sedangkan jumlah trombosit tidak menunjukkan perbedaan yang signifikan (P>0,05) di antara kelompok perlakuan. Kondisi sistemik tubuh kucing kelompok II dan III lebih baik dibandingan kelompok I.
Ekstrak Daun Singkong Baik Sebagai Antioksidan pada Burung Puyuh Dewasa yang Mendapat Paparan Panas Singkat (EXTRACT OF CASSAVA LEAVES IS A GOOD ANTIOXIDANT FOR MATURE QUAIL WHICH EXPOSED TO HEAT IN SHORT TIME) La Jumadin; Aryani Sismin Satyaningtijas; Koekoeh Santoso
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.588 KB) | DOI: 10.19087/jveteriner.2017.18.1.135

Abstract

The aims of this study was to investigate the potential use of chlorophyll extracted form cassava leaves as antioxidant for quail (Coturnix coturnix japonica). The experimental design adopted in this study was simple randomized design consisting of six treatments. The six treatments consist of chlorophyll extract at the dose 5,29 mg/168 g body weight (KL), animal exposed to 40oC for 8 hours daily (P), animal exposed to 40oC for 8 hours daily treated with chlorophyll extract at the dose of 5,29 (P+KL1), at the dose of 10,58 (P+KL2, and at the dose of 21,16 mg/168 g (P+KL3). The treatment was conducted for 28 days following 7 days adaptation period. Parameters observed in this study was feed consumption, feed digestibility, body weight, the number of eggs, egg weight, the level of yolk, the level of albumin, the weight of ovaries and uterus, and the thickness of egg shell. The results showed that quail exposed to 40oC for 8 hours daily (P) had a lower level of feed consumption as compared to those of animal unexposed to 40oC heat (P0). The highest level of feed digestibility was observed in KL treatment group. The average body weight tended to increase in quail treated with chlorophyll (KL) as compared to those of K0 and P treatment groups. The number of eggs and egg weight increased in quail exposed to 40oC and treated with chlorophyll extract. The level of yolk and albumin tend to increase in quail treated chlorophyll (P+KL) as compared to those of K0 and P. The average weights of ovary and uterus were not significantly different among all treatment groups (P>0,05). The thinnest egg shell was observed in P treatment group. The result of this study shows that the chlorophyll has antioxidant potential for adult quail exposed briefly to heat. ABSTRAK Penelitian ini bertujuan mengkaji potensi ekstrak klorofil daun singkong sebagai antioksidan pada burung puyuh (Coturnix coturnix japonica) dewasa yang dipapar panas singkat. Adapun peubah yang diamati adalah konsumsi pakan, kecernaan pakan, bobot badan, jumlah telur, bobot telur, tinggi kuning telur, tinggi putih telur/albumen, bobot ovarium, bobot uterus, dan tebal kerabang telur. Penelitian ini terdiri dari enam kelompok perlakuan. Kelompok kontrol (K0), kelompok hewan uji hanya diberi ekstrak klorofil daun singkong 5,29 mg/168 g bobot badan/oral (KL). Kelompok hewan uji dipapar suhu 40oC selama delapan jam tiap hari (P). Kelompok P+KL1, P+KL2, dan P+KL3 masing-masing dipapar suhu 40oC selama delapan jam tiap hari, kemudian diberi ekstrak klorofil daun singkong 5,29, 10,58, dan 21,16 mg/168 g bobot badan per oral selama 28 hari setelah diadaptasikan satu minggu. Parameter seperti konsumsi pakan, kecernaan pakan, jumlah telur, dan bobot telur dihitung setiap hari selama penelitian. Parameter lain diukur pada akhir perlakuan, kecuali bobot badan dilakukan setiap minggu. Hasil penelitian menunjukkan bahwa burung puyuh yang mendapatkan paparan panas (P) konsumsi pakannya cenderung menurun dibandingkan kelompok K0. Rataan kecernaan pakan tertinggi dijumpai pada kelompok KL. Rataan bobot badan burung puyuh yang mendapatkan ekstrak klorofil daun singkong cenderung meningkat dibandingkan dengan perlakuan K0 dan P. Rataan jumlah dan bobot telur pada kelompok yang mendapatkan paparan panas dan ekstrak klorofil daun singkong cenderung meningkat dibandingkan kelompok K0, kecuali pada kelompok P+KL3. Rataan tinggi kuning telur/yolk dan albumen dari kelompok burung puyuh yang mendapat ekstrak klorofil daun singkong cenderung lebih tinggi dibandingkan kelompok K0 dan P. Rataan bobot ovarium dan uterus pada semua kelompok perlakuan tidak menunjukkan perbedaan (P>0,05). Rataan tebal kerabang telur pada kelompok P paling tipis dibandingkan yang lain. Simpulan pada penelitian ini adalah ekstrak daun singkong memiliki potensi sebagai antioksidan pada burung puyuh dewasa yang diberikan paparan panas singkat.
Kualitas Semen Beku Babi dalam Pengencer Komersial yang Disuplementasi dengan Trehalosa (THE QUALITY OF BOAR FROZEN SEMEN IN COMMERCIAL EXTENDER SUPPLEMENTED WITH TREHALOSE) Tuty Laswardi Yusuf; Raden Iis Arifiantini; Reni Ratni Dapawole; Wilmientje Marlene Mesang Nalley
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (105.27 KB) | DOI: 10.19087/jveteriner.2017.18.1.69

Abstract

The aim of the research was to study trehalosa supplementation in Beltsville Thawing Solution (BTS) and MIII extender on the quality of frozen boar semen. Semen was collected from nine boars age 5-6 years using glove hand method. The semen were then evaluated macro and microscopically. Semen having >70% sperm motility and >200x106/mL sperm concentration were diluted in two tubes containing BTS and two tubes containing MIII, then kept at room temperature (20-22oC) for 2 hours (holding time). Following this the semen were centrifugated at 2000 RPM for 10 minutes and the supernatant was discarded, the pellet was re-diluted with BTS, BTS-Trehalose 100 mM (BTS-T), MIII, and MIII-Trehalose 100 mM (MIII-T) with 4% glycerol, packed into 5 mL macrotube, equilibrate at 5oC for 2 hours. After equilibration macrotube were frozen horizontally 4 cm above the liquid nitrogen vapor for 20 minutes and then submerged in liquid nitrogen (-196oC) for futher evaluation. After 24 hours the semen than thawed at 52oC for 45 seconds. The results demonstrated that sperm motility in BTS-T (30.00±4.47%) was higher (P<0.05) then MIII-T (26.67±8.16%), BTS (24.17±9.17%), or MIII (21.67±7.53%). This research conclude that suplementation of 100 mM trehalosein BTS extender improved motility of boar sperm after freezing and thawing. ABSTRAK Penelitian ini bertujuan untuk mempelajari penambahan trehalosa dalam pengencer Beltsville Thawing Solution (BTS) dan MIII terhadap kualitas semen beku babi. Semen dikoleksi dari sembilan ekor babi berumur 5-6 tahun menggunakan teknik glove hand method. Semen dievaluasi secara makro dan mikroskopis. Semen yang memiliki spermatozoa motil di atas 70% dengan konsentrasi di atas 200x106/mL dibagi ke dalam empat tabung, dua tabung pertama diencerkan dengan BTS dan dua tabung lainnya dengan MIII. Semen yang telah diencerkan disimpan pada suhu ruangan (20-22oC) selama dua jam (holding time). Semen kemudian disentrifugasi 2000 rpm selama 10 menit. Supernatan dibuang dan pellet diencerkan kembali. Semen yang disentrifugasi dengan BTS, diencerkan dengan BTS gliserol (BTSg) dan BTS gliserol Trehalosa 100 mM (BTSgT) dan yang disentrifugasi dengan MIII diencerkan dengan MIIIg dan MIIIgT. Gliserol yang digunakan adalah 4%. Semen yang telah diencerkan dikemas dalam macrotube (5 mL), diekuilibrasi pada 5oC selama dua jam. Setelah equilibrasi macrotube dibekukan 4 cm di atas permukaan nitrogen cair selama 20 menit dan disimpan dalam container nitrogen cair (-196oC) untuk pengujian lebih lanjut. Setelah penyimpanan 24 jam, terhadap semen beku dilakukan thawing pada suhu 52oC selama 45 detik. Hasil penelitian menunjukkan motilitas spermatozoa dalam BTSgT (30,00±4,47%) paling tinggi (p<0,05) daripada MIIIgT (26,67±8,16%), BTSg (24,17±9,17%), atau MIIIg (21,67±7,53%). Simpulan dari penelitian ini adalah penambahan 100 mM trehalosa dalam pengencer BTSg dapat mempertahankan motilitas spermatozoa babi lebih baik daripada pengencer lainnya.
Parasitic Worm in Tiger (Panthera tigris) at Serulingmas Zoological Garden Banjarnegara, Bandung Zoological Garden, and Indonesia Safari Park Bogor Risa Tiuria; Unita Pratiwi; Ligaya ITA Tumbelaka
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.4 KB) | DOI: 10.19087/jveteriner.2017.18.1.1

Abstract

This research was done to infestigate the existence and the type of parasitic worms from feces of tiger (Panthera tigris) at Serulingmas Zoological Garden (TRMS) at Banjarnegara, Central Java , Bandung Zoological Garden (KBB), and Indonesia Safari Park Bogor (TSI). Total of 35 tigers feces samples were examined. They are taken from 4 Bengal tigers at Serulingmas Zoological Garden, 12 tigers (8 Bengal tigers and 4 Sumatran tigers) at Bandung Zoological Garden, and 19 tigers (4 Bengal tigers and 15 Sumatran tigers) at Indonesia Safari Park Bogor. All of the feces samples were examined with qualitative (flotation and sedimentation) and quantitative (McMaster slide) method to know the existence of parasitic worm eggs. Moreover, a tiger feces that contain eggs of strongylid were cultured. Parasitic worms that were found in tigers from the research were ascarid (Toxocara sp, Toxascaris sp), strongylid (Trichostrongylus sp, Ancylostoma sp, Cooperia sp), ), oxyurid (Oxyuris sp) and Strongyloides sp. The result showed that prevalence index of parasitic worms in tigers at TRMS, KBB, and TSI were 100%, 50%, and 47,4%, respectively. Parasitic worms at TRMS were ascarid (Toxocara sp), strongylid (Ancylostoma sp, Trichostrongylus sp, Cooperia sp) and Strongyloides sp. Parasitic worms at KBB were ascarid (Toxocara sp, Toxascaris sp), strongylid (Ancylostoma sp, Trichostrongylus sp), dan oxyurid (Oxyuris sp). Parasitic worms at TSI were ascarid (Toxocara sp, Toxascaris sp), strongylid (Ancylostoma sp), and oxyurid (Oxyuris sp). ABSTRAK Penelitian ini dilakukan untuk mengetahui jenis cacing parasitik pada harimau (Panthera tigris) di Taman Rekreasi Margasatwa Serulingmas (TRMS) Banjarnegara Jawa Tengah, Kebun Binatang Bandung (KBB), dan Taman Safari Indonesia (TSI) Bogor. Sebanyak 35 sampel tinja harimau dari tiga lembaga konservasi eks-situ, yaitu 4 ekor harimau Benggala dari TRMS, 12 ekor (4 ekor harimau Benggala dan 8 ekor harimau Sumatera) dari KBB, dan 19 ekor (4 ekor harimau Benggala dan 15 ekor harimau Sumatera) dari TSI Bogor. Semua sampel tinja diperiksa dengan metoda kualitatif (pengapungan dan sedimentasi) dan kuantitatif (metoda McMaster). Selain itu juga dilakukan pemupukan pada tinja yang positif telur strongylid. Cacing parasitik yang ditemukan dari penelitian ini adalah cacing ascarid (Toxocara sp, Toxascaris sp), cacing strongylid (Trichostrongylus sp, Ancylostoma sp, Cooperia sp), cacing oxyurid (Oxyuris sp) dan Strongyloides sp. Berdasarkan hasil penelitian, dapat disimpulkan bahwa prevalensi kecacingan pada harimau di TRMS 100%, di KBB 50%, dan di TSI Bogor 47,4%. Cacing parasitik yang ditemukan pada harimau di TRMS adalah cacing ascarid (Toxocara sp), cacing strongylid (Ancylostoma sp, Trichostrongylus sp, Cooperia sp) and Strongyloides sp. Cacing parasitik yang terdapat pada harimau di KBB adalah cacing ascarid (Toxocara sp, Toxascaris sp), cacing strongylid (Ancylostoma sp, Trichostrongylus sp), dan cacing oxyurid (Oxyuris sp). Cacing parasitik yang terdapat di TSI Bogor adalah cacing ascarid (Toxocara sp, Toxascaris sp), strongylid (Ancylostoma sp), dan cacing oxyurid (Oxyuris sp).
Ayam Pedaging Jantan yang Dipelihara di Dataran Tinggi Sulawesi Selatan Produktivitasnya Lebih Tinggi (HIGHER PRODUCTIVITY PERFORMANCE OF MALE BROILERS REARED IN THE HIGHLANDOF SOUTH SULAWESI) Bahri Syamsuryadi; Rudi Afnan; Irma Isnafia Arief; Damiana Rita Ekastuti
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.432 KB) | DOI: 10.19087/jveteriner.2017.18.1.160

Abstract

Study to determine the correlation between husbandry environment and sex to the performance, hematological characteristics, and meat quality of broilers have been conducted. Two hundred and eighty eight female and male Cobb broilers, were kept in cage where eight broilers with the same sex in each pen. A completely randomized experimental design with a 3x2 factorial arrangement (three different altitudes x two sexes) with three replicates was applied. The three different altitudes included 50 m, 300 m, and 500 m above sea level, respectively. The results showed that differences in maintenance altitude and sex significantly influenced (P<0.01) the panting frequency, water and feed consumption, body weight, physical meat traits (pH and cooking loss), and meat microbiology. Whilst, differences in maintenance altitude with broilers of the same sex significantly did not affect (P>0.01) the animal srectal temperature, feed conversion, and meat chemical and organoleptic. It is concluded that better productivity can be achieved when male broilers are reared in a high altitude environment. ABSTRAK Penelitian ini bertujuan untuk mengkaji hubungan lingkungan pemeliharaan dan jenis kelamin ayam pedaging terhadap performans, karakteristik hematologi, dan kualitas daging. Sebanyak 288 ekor ayam pedaging strain Cobb, berjenis kelamin jantan dan betina, dipelihara dalam petak kandang dan tiap petak diisi delapan ekor ayam dengan jenis kelamin yang sama. Penelitian disusun berdasarkan Randangan Acak Lengkap (RAL) pola faktorial (3x2) dengan tiga ulangan. Faktor pertama ialah ketinggian tempat pemeliharaan dengan tiga kategori yaitu: ketinggian (50 mdpl), ketinggian (300 mdpl), dan ketinggian (500 mdpl) sedangkan faktor kedua ialah jenis kelamin jantan dan betina. Hasil penelitian menunjukkan perbedaan ketinggian tempat pemeliharaan dan jenis kelamin nyata memengaruhi (P<0.01) frekuensi panting, konsumsi air minum, konsumsi pakan, bobot badan, fisik daging (pH dan susut masak), dan mikrobiologi daging. Pemeliharaan pada ketinggian 50, 300, dan 500 mdpl dengan jenis kelamin berbeda nyata tidak memengaruhi (P>0.01) suhu rektal, konversi pakan, kimia daging dan organoleptik. Hasil penelitian ini menyimpulkan performans yang lebih baik dapat dicapai apabila ayam pedaging dipelihara pada dataran tinggi dengan jenis kelamin jantan.
Evaluasi Penerapan Standar Sanitasi dan Higien di Rumah Potong Hewan Kategori II (EVALUATION OF SANITATION AND HYGIENE STANDARD IMPLEMENTATION AT CATEGORY II ABATTOIR) Zikri Maulina Gaznur; Henny Nuraini; Rudy Priyanto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.66 KB) | DOI: 10.19087/jveteriner.2017.18.1.107

Abstract

Meat is one of livestock industry products from abattoir. The existence of abattoir is necessary to ensure the meat product produced is safe, healthy and halal. This study was conducted to evaluate sanitation and hygiene standard implementation in category II abattoir. Total plate count (TPC), Salmonella sp, Coliform, and Escherichia coli were analized by using Bacteriological Analytical Manual (BAM) method. Analisis of water quality and liquid waste was done according to American Public Health Association (APHA) method. Based on laboratory test on TPC, Salmonella sp, Coliform, and Escherichia coli, the test results did not exceed the limit standard of SNI 3932:2008. The result of water quality and liquid waste analysis was around the threshold set by Indonesian Republic’s Regulation of Health Ministry No. 492/Menkes/Per/IV/2010 on the Quality Requirements of Water and Environment Regulation Ministry No. 5/2014 regarding Standard Liquid Waste. ABSTRAK Daging adalah salah satu produk industri peternakan dari usaha pemotongan hewan. Permintaan masyarakat terhadap daging sapi memengaruhi intensitas pemotongan, sehingga keberadaan rumah pemotongan hewan (RPH) diharapkan dapat menjamin kualitas daging secara aman, sehat dan halal. Penelitian ini bertujuan untuk mengevaluasi penerapan standar sanitasi dan higien di RPH kategori II. Pengujian total plate count (TPC) daging, Colliform, Escherichia coli, dan Salmonella sp berdasarkan metode Bacteriological Analytical Manual (BAM). Pengujian air bersih dan limbah cair menggunakan metode American Public Health Association (APHA). Hasil analisis mikrobiologi menunjukkan bahwa TPC, Salmonella sp, Coliform dan E. coli berada di bawah persyaratan SNI 3932:2008. Hasil analisis air bersih dan limbah cair sudah memenuhi baku mutu Peraturan Menteri Kesehatan Republik Indonesia 492/Menkes/Per/IV/2010 tentang Persyaratan Kualitas Air Bersih dan Peraturan Menteri Lingkungan Hidup 5/2014 Tentang Baku Mutu Air Limbah.

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