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I G. Made Krisna Erawan
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
Diet Rendah Protein Meningkatkan Jumlah Neuron Nitrergik Duodenum dan Jejunum Serta Menurunkan Bobot Badan Tikus Wistar (LOW PROTEIN DIET INCREASES THE NUMBER OF DUODENUM, JEJUNUM NITRERGIC NEURONS AND REDUCES BODY WEIGHT IN THE WISTAR RATS) Raden Rara Amelia Hana; Sarmin Sarmin; Claude Mona Airin; Pudji Astuti
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.663 KB) | DOI: 10.19087/jveteriner.2018.19.4.539

Abstract

Protein is very important for the growth, development and activity in rats. The impact of lack protein intake to gastrointestinal, especially in the small intestine, the number of nitrergic neurons, and changes in body weight in Wistar rats has not been studied. Therefore the aims of this study to examine the effect of a low-protein diet for 4 weeks on the number of duodenal, jejunal, ilealnitrergicneuron and changes in body weight in Wistar rats. Nine male Wistar (Rattusnorvegicus) rats, aged 1 month, with an average body weight of 120 grams used in this study. The rats were adapted for 7 days in individual cages with a normal diet (containing 24% protein) and drinking ad libitum. After being adapted all rats were divided into 3 groups (K-24, K-14, and K-4), consist of 3 rats. Then all rats fasted for 12-hours. Each group received treatment as follows: K-24 group (as a control) was given a diet containing 24% of protein, K-14 group was given a diet containing 14% of protein, and K-4 group was given a diet containing 4% of protein . Diet and drinking water are given ad libitum. Along of treatment for 4 weeks.body weights and blood glucose were measured every weeks, then all rats were euthanized and sample tissues of duodenum, jejunum, and ileum were then were obtained for histopathological preparations using the NADPH-d technique. Data on the number of nitrergicmyenteric neurons in the duodenum, jejunum, and ileum, body weight gain, and blood glucose levels were statistically analyzed by single factor design Anova. The results of this study concluded that a low protein diet (14% and 4% protein) for 4-weeks in the duodenum, jejunum have been increased the number of nitrergic neurons, on the other hand, in the ileum have been decreased the number of nitrergic neurons, reduced body weight, and did not increase blood glucose levels in Wistar rats.
Pneumonia Verminosa pada Kucing Lokal yang Terinfeksi oleh Aelurotsrongylus sp (VERMINOUS PNEUMONIA IN DOMESTIC CAT INFECTED BY AELUROSTRONGYLUS SP) Ida Bagus Oka Winaya; I Ketut Berata; I Made Kardena; Ida Bagus Made Oka
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to evaluate the pulmo pathological changes of domestic cat infected byAelurostrongylus sp. A total of 15 cats were examined at Faculty of Veterinery Medicine, Udayana Universityduring 2010. Ten out of 15 cats showed sneezing, whereas the remains showed serous rhinitis and sneezing.Macroscopic and microscopic changes were observed mainly on pulmo samples. Hyperemias on caudalislobes and pleura effusion were found in the pulmo. The pulmo tissue was fixed on 10 % neutral bufferformalin and stained with haematoxilin-eosin (HE) for histopathological examination. Aelurostrongylus spwas present in the alveoli lumen of the lung samples. Meanwhile, the alveoli septa of the lung wereobserved thicker and infiltrated with neutrophils, plasma exudates and erythrocytes. Pleural effusion wasmainly consisted of eosinophilic substances. It is concluded that verminous pneumonia in domestic catinfected with Aelurostrongylus sp was an acute infection.
Penerapan Metode Diagnosis Cepat Virus Avian Influenza H5N1 dengan Metode Single Step Multiplex RT-PCR Aris Haryanto; Ratna Ermawati; Medania Purwaningrum; Dini Wahyu Yudianingtyas; Michael Haryadi Wibowo; Charles Rangga Tabbu
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian influenza (AI) virus is a segmented single stranded (ss) RNA virus with negative polarity andbelong to the Orthomyxoviridae family. Diagnose of AI virus can be performed using conventional methodsbut it has low sensitivity and specificity. The objective of the research was to apply rapid, precise, andaccurate diagnostic method for AI virus and also to determine its type and subtype based on the SingleStep Multiplex Reverse Transcriptase-Polymerase Chain Reaction targeting M, H5, and N1 genes. In thismethod M, H5 and NI genes were simultaneously amplified in one PCR tube. The steps of this researchconsist of collecting viral RNAs from 10 different AI samples originated from Maros Disease InvestigationCenter during 2007. DNA Amplification was conducted by Simplex RT-PCR using M primer set. Then, bysingle step multiplex RT-PCR were conducted simultaneously using M, H5 and N1 primers set. The RTPCRproducts were then separated on 1.5% agarose gel, stained by ethidum bromide and visualized underUV transilluminator. Results showed that 8 of 10 RNA virus samples could be amplified by Simplex RTPCRfor M gene which generating a DNA fragment of 276 bp. Amplification using multiplex RT-PCRmethod showed two of 10 samples were AI positive using multiplex RT-PCR, three DNA fragments weregenerated consisting of 276 bp for M gene, 189 bp for H5 gene, and 131 bp for N1. In this study, rapid andeffective diagnosis method for AI virus can be conducted by using simultaneous Single Step Multiplex RTPCR.By this technique type and subtype of AI virus, can also be determined, especially H5N1.
Pemberian Kalsium Laktat dan Berenang Meningkatkan Osteoblast pada Epiphysis Tulang Radius Mencit Perimenopause (ORALLY LACTATE CALCIUM AND SWIMMING INCREASE OSTEOBLAST IN EPIPHYSIS RADIAL PERIMENOPAUSE MICE BONE) Muliani .; I Nyoman Mangku Karmaya; Ketut Tirtayasa
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study is to measure the effect(s) of orally administered calcium lactate and swimmingactivity to the increase of osteoblasts in the epiphysis of radial bone at perimenopause stage. We usedmice of Mus musculus species age 15-16 months as experimental animals in this particular study. Forexperimental design we used Pre-test and Post-test Control Group Design. There were 4 experimentalgroups: Control; Calcium lactate administration; Swimming activity; and Combination of calcium lactateand swimming activity. Each group contained 22 mice and treatments were given to each group for sixweeks (42 days). Our results showed that following treatment, the average numbers of osteoblasts were9.46±1.81; 26.58±2.82; 25.05±2.68; 59.38±2.91 in Control, Calcium lactate administration, Swimmingactivity, and Combination of calcium-lactate administration and swimming activity groups respectively.There was a significant difference in average osteoblasts between the Control group and the other 3 groups(p<0.05) and there was no significant difference between the Calcium lactate administration and Swimmingactivity groups (p>0.05). The increase number of osteoblasts in the Combination group was larger than theother groups. In conclusion, administration of calcium lactate or swimming activity could increase thenumber of osteoblasts in mice, and the increase is larger when applied in combination.
Kurva Pertumbuhan Sapi Friesian Holstein dari Lahir Sampai Siap Kawin Berdasarkan Tingkat Kelahiran (HOLSTEIN GROWTH CURVE OF NEW BORN CALF UNTIL FIRST MATING BASED ON BIRTH RATE) Lia Budimulyati Salman; Cece Sumantri; Ronny Rachman Noor; Asep Saefuddin; Chalid Talib
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The growth curve is a reflection of the ability of an individual or population to actualize themselves aswell as the size will be the development of the parts of the body until it reaches the maximum size (adult)on the existing environmental conditions. The main goal of this study is to determine the growth curvemodel of Friesian Holstein cattle from birth until ready to mate based on birth rates with Logistic model,Gompertz, and von Bertalanffy. The data was used in this study is the result of the weighing of dairy cowsbody weight of female Friesian Holstein as many as 335 head number collected by PT Taurus Dairy FarmSukabumi from 2001to 2011, which have complete data from birth until first mating with a birth rate thatis different from the birth of the one to five. The data used in the analysis of growth curves using three nonlineargrowth curve model i.e. Logistic models, Gompertz, and Von Bertalanffy. The results obtainedindicate that the growth curve model shave different levels of accuracy, depending on the environment andage or duration of observation, the longer observed to mature weight (A), the age of puberty, and pubertygreater weight. The Conclusion of this study is a model of Logistic close to field conditions. Morover thehigher the birth rate will be heavier birth weight and adult weight. These three non-linear mathematicalmodel used in this study has a high degree of accuracy.
Peningkatan Kandungan Kalium Urin Setelah Pemberian Ekstrak Sari Buah Belimbing Manis (Averrhoa carambola) (THE INCREASE OF POTASSIUM URINE CONTENT AFTER ADMINSTRATION OF CARAMBOLA (AVERRHOA CARAMBOLA) FRUIT JUICE EXTRACT) Ruqiah Ganda Putri Panjaitan; Maria Bintang
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Carambola (Averrhoa carambola L.) has been used as medicinal plant. This research has beenconducted to study the potential diuretic of fruit juice carambola extract on male rats. Diuretic activitywas tested by using Cumming’s method. The treatment was administered only once, and the urine up to 24hours after treatment was collected. The result shows that the administration of 1.6 mL/100 g body weightof fruit juice carambola extract resulted in lower urine volume compared to the without treatment orklortalidon at dose 0.315 mg/100 body weight (p>0.05). Furthermore, Na+ content in treatment rats’ wasurine lower compared to the without treatment or klortalidon (p<0.05). in contrast, high content of K+ wasobserveb in treatment  rast’ urine compared to the without treatment or klortalidon (p> 0.05). It is concludedthat the administration of carambola fruit juice extract may increase K+ content in urine and produce moreconcentrated urine. The mechanism of action, however, remains need to be proven, further.
The Production and Use of Monoclonal Antibodies for the Detection of Avian Influenza Antigen in the in Infected Chickens Nyoman Mantik Astawa; Ida Bagus Kade Suardana; Gusti Ayu Yuniati Kencana
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A safe and appropriate diagnostic method for avian influenza virus (AIV) infection in chickens wasestablished using monoclonal antibodies (mAbs) against the virus. The virus used for the production of themonoclonal antibodies was an Indonesian AIV-H5N1 isolate. Immortal mouse myeloma cells were fusedwith the lymphocytes derived from the spleen of mice immunized with the virus. The mAbs were tested fortheir specificity by enzyme linked immunosorbent assay (ELISA) and western blotting using formaldehydeinactivated virus and normal allantoic fluid as antigens. Twelve mAbs specific against AIV were isolatedand 8 mAbs were used for immunodetection of AIV antigen in chicken’s tissues. By indirect ELISA, themAbs were able to detect AIV antigen in allantoic fluid at the titre as low as 2-2 to 2-4 HA units per 0.1 ml.By immunoperoxidase staining AIV–antigen was detected in paraffin embedded tissues of AIV-infectedchickens. AIV antigen was not detected in chickens which were confirmed to be AIV negative. In theinfected chickens, high intensity of AIV antigen was detected in proventricle gland and small intestine.The AIV antigen with a lesser intensity was detected in lungs and spleen but hardly detected in muscle,brain and several other tissues. This study show clear evidences that mAbs produced in this study areapplicable for use in the detection of AIV antigen in infected chickens.
Variasi Respon Pembentukan IgY terhadap Toxoid Tetanus dalam Serum dan Kuning Telur pada Individu Ayam Petelur I Wayan Teguh Wibawan; Iman Bayu Prakoso Darmono; I Nyoman Suartha
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The variation of response on the production of specific IgY to tetanus toxoid among chickenin serum and egg yolk was observed in this study. Chicken showed relatively late response inproducing specific IgY in serum, around 59 days were needed to have a positive precipitationreaction of complex IgY-tetanus toxoid in the immunodiffusion test (agar gel precipitation test/AGPT). The presence of IgY in egg yolk could be detected one week after positive reations inserum was observed. The positive reaction in AGPT mostly related with the positive reaction inELISA, eventhough the variation of titer values were observed among chicken sera and egg yolk.This response variation might be related with the different of physiological status of the chicken.
Pemberian Ekstrak Methanol Daun Paliasa Menurunkan Kadar Glukosa Darah Tikus Hiperglikemik (METHANOL EXTRACT OF PALIASA LEAVES DECREASES BLOOD GLUCOSE LEVEL HYPERGLYCEMIC RATS ) Yuliana .; Tangking Widarsa; Gede Wiranatha
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Diabetes melitus is a disease that often encountered by Indonesian people. It can cause microvascular andmacrovascular complication. Traditional herbal medicine such as paliasa (Kleinhovia hospita L.) leavesextract can decrease blood glucose. This study was aimed to identify the effectiveness of extract paliasaleaves on blood glucose decreasing for aloxan induced Wistar mice. This Control Group Design used twentyeight mice (three months old). The mice were divided randomly into four groups, i.e. control, paliasa250mg/kg body weight (BW), 500 mg/kg BW, and 750 mg/kg BW. After one week adaptation and induced byalloxan, pretest fasting blood glucose test was done. Treatment was given for two weeks and posttest bloodglucose test was done afterwards. The decrease of fasting blood glucose level was analyzed by usinganalysis of varians. Result showed there was significantly higher level of blood glucose decrease in micewith paliasa extract dose of 750mg/kgBB. Mean level of blood glucose decrease on control group was2.9±0.17, paliasa group with dose 250mg/kgBB was 29.28±1.15, paliasa 500 mg/kgBB was 46.7±2.1, danpaliasa 750mg/kgBB was 74.8±1.88.  In conclusion, extract paliasa treatment can decrease fasting bloodglucose level on hyperglycemic rats.
Analisis Genetik Gen Protective Antigenic pada Bacillus anthracis Isolat Jawa Tengah dan Yogyakarta (GENETIC ANALYSIS ON PROTECTIVE ANTIGENIC GENE OF BACILLUS ANTHRACIS ISOLATES OF CENTRAL JAVA AND YOGYAKARTA) Maxs Urias Ebenhaizar Sanam; Widya Asmara; Agnesia Endang Tri Hastuti Wahyuni; Michael Haryadi Wibowo
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to determine sequence and genotype diversity of protective antigenic gene ofBacillus anthracis isolated from Central Java and Yogyakarta. Pag-A gene which encodes for antigenicprotein is one toxin component and the virulent factor of B. anthracis. As many as five isolates fromSemarang, Sragen, and Boyolali (Central Java) and Sleman (Yogyakarta) were used. The gene wassequenced and amplified used three set of primers PA1857/PA2436, PA8/PA5, and PA-5F/PA-5R. Theresult showed that the nucleotide sequences of gene from five isolates were identical and only had onenucleotide difference as compared to B. anthracis sterne M22589. All isolates were confirmed as genotypebased on pag-A sequence. It was concluded that all B. anthracis from Central Java and Yogyakarta haveidentical pag-A sequence and belong to genotypt-1. Further studies are needed to investigate B. anthracisisolates from other regions of Indonesia.

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