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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Ragam Jenis Nyamuk di Sekitar Kandang Babi dan Kaitannya dalam Penyebaran Japanese Encephalitis Upik Kesumawati Hadi; Susi Soviana; Tatty Syafriati
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The mosquitoes species in pig pen area and its relation to the trasmission of Japanese Encephalitis(JE) in North Sumatra Province was studied as the first step of the investigation on JE prevalence inIndonesia. The aim of this research is to determined the mosquitoes species that can be a potential vectorsof JE and its relation to the prevalence of JE reactor of pigs in North Sumatra. Mosquito collections werecarried out by using ultra violete light trap, Magoon trap and human landing collections. Serological studywas done by using competitive enzyme linked-immunosorbent assay (C-ELISA) methode. The resultsshowed several mosquitoes species i.e. Culex tritaeniorhynchus, Cx. quinquefasciatus, and Cx. fuscocephalusthat can be a potential vectors of JE was found in this province. The environmental conditions and the pigfarm and horses stable management supported the development of ideal breeding places for mosquitoesand other blood sucking flies. It was reported also that the prevalence of JE reactors in pig farms and horsestable in North Sumatra was high (71.67%). The high prevalence of JE reactors indicated that the infectionof JE was actively occured between pigs-mosquitoes-pigs in thise area.
Patogenisitas Streptokokus Grup B pada Mencit Neonatus Zinatul HayatiO; Teuku Fadrial Karmil
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Group B Streptococci (GBS) are the major cause of serious infections in neonates, including pneumonia,septicemia and meningitis. Considering that GBS bacteria founded in Indonesia in our previous study isdissimilar as what have been found in foreign country particularly in its serotype distribution, thereforethis research is extremely essential to be conducted in Indonesia in order to searching the vaccine candidateto be used in Indonesia. This research focused in pathogenicity test of each GBS isolates on animal model.Pathogenicity experiment has been done by early onset septicemia model approach to neonatal mouse.Pathogenicity test result that SR-7 isolates can cause the death of neonatal mouse 100% in early-onsetinfection. Whereas, SV-2 isolates cannot cause the neonatal mouse neither in early-onset nor in late-onset.From the serotype determination result at previous research found that SR-7 isolates is serotype isolatesVI whilst SV-2 isolates is serotype III. This result has shown that SR-7 (serotype VI) isolates is the mostpathogen and the SV-2 isolates is less pathogen.
Peningkatan Kadar Protein Putak melalui Fermentasi oleh Kapang Trichoderma reesei (THE INCREASE OF PROTEIN LEVEL FROM PUTAK THROUGH FERMENTATION OF FUNGI TRICHODERMA REESEI) Maritje Aleonor Hilakore; Suryahadi .; Komang Wiryawan; Djumali Mangunwijaya
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted was to increasing the protein level in putak by fermentation using fungiTrichoderma reesei. A laboratoryum experimental study was conducted using  factorial CompletelyRandomized Design 3 x 4 x 3. The main factor is were  inoculant levels of fungi T. reesei (T): 5,0; 7,5 and 10,0% (w/w),the level  and the second factor were of incubation time (W):  2; 3; and 4 days. Variables tested werecrude protein (CP), true protein (TP) and crude fiber (CF). The result showed that  treatment with 7.5% ofT.reesei  and incubation time for 4 days gawe the highest of crude and true protein level (20,60%)  from14,17% and 13,25% from 3,25%, and  lowest crude fiber 9,08% from 9,70%. Through fermentation of  fungiT.reesei can be increase the protein and decrease the fiber level of putak.
Stabilitas Mikrob Usus, Histologi Hati dan Ginjal Mencit Setelah Pemberian Ekstrak Pliek u Bumbu Masak Tradisional Aceh (STABILITY OF GUT MICROBIAL AND HISTOLOGY OF LIVER AND KIDNEY OF MICE AFTER ADMINISTRATION OF PLIEK-U EXTRACT Nurliana .; Sri Estuningsih; Sugito .; Dian Masyitha
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Pliek u is one of the traditional spices of Aceh prepared by fermentation of coconut meat which is usedas chicken feed additive too. The stability of gut micro flora and histopathological changes of liver andkidney of mice were detected after three days oral administration of acute single dose of ethanol extract ofpliek u. Nine animals were used and grouped into three; in which group I and II (treatment groups) wereadministered 370 and 733 mg/kg body weight of pliek u, respectively, and group III referred as the control.At the fourth day of experiment, all animals were sacrificed, and their livers, kidneys and intestinal gutwere excised. The gut microbial was measured by Total Plate Count (TPC). Livers and kidneys wereprocessed for paraffin procedure and hematoxyllin-eosin staining. The results showed that the ethanolextract of pliek u has no significant effect (P>0.05) on the TPC of the gut microbial and the structure of theliver and kidney of mice. It can be concluded that administration of the two doses (370 and 730 mg/kg bw)of pliek u did not lowering the numbers of gut microbial and were not toxic to the livers and kidneys of themice.
Imunitas Protektif Mencit Terhadap Cairan Kista Taenia saginata (PROTECTIVE IMMUNITY OF MICE AGAINST CYST FLUID OF TAENIA SAGINATA) Nyoman Sadra Dharmawan; I Made Dwinata; I Made Damriyasa; Ida Bagus Made Oka; Kadek Swastika; Luh Dewi Anggreni; Nyoman Mantik Astawa
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to determine immune response of mice against vaccines derived fromcyst fluid of Taenia saginata. The study was conducted using four BALB/c mice aged 6 weeks as experimentalanimals. All experimental animals were vaccinated intra peritoneal with Taenia saginata cyst fluidemulsified in Freund’s adjuvant. Immune response in the mice was determined by detecting antibodiesusing ELISA and by the presence of lymphocytes through evaluation of blood smear. The results showedthat the cyst fluid of Taenia saginata was antigenic and capable of inducing antibody responses that weredetected by ELISA. Mean antibody titers obtained in the results of the first, second, third, and fourth ofvaccination was 3.3 units; 17.9 units; 21.2 units; and 72.1 units; respectively. Evaluation of blood smear ofvaccinated mice showed an increase in the percentage of lymphocytes after vaccination with an average66.75%, compared with the average of lymphocytes before vaccination which was 40.75%. Further researchis still required in experimental animals by vaccination followed by challenge test with Taenia saginataeggs.
Lymphocytes Subpopulation in Peripheral Blood and Spleen of Village Chickens Recognized by Monoclonal Antibodies (SUBPOPULASI LIMFOSIT PADA DARAH TEPI DAN LIMPA AYAM KAMPUNG YANG DIKENALI OLEH ANTIBODI MONOCLONAL) Nyoman Mantik Astawa; Ida Bagus Made Oka
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lymphocytes play important role in host defence system against pathogenic agents both in mammalianand avian species. Monoclonal antibodies (mAbs) have been widely used to identify lymphocytessubpopulation in a host based on their surface cluster differentiation (CD) markers. Currently, mAbsagainst lymphocytes surface markers of village chickens have been produced by fusion of myeloma withlymphocytes derived from spleen of mice immune to chicken lymphocytes. In two fusion experiments, 623clones of hybridomas were produced and four (BG4, CB1, DB2 and BB2) of which secreted mAbs againstchickens lymphocyte surface molecules. Two mAbs (BG4 and DB2) recognized protein of 32 kDa, one mAb(CB1) recognized protein of 64 kDa, and one mAb was unable to recognize any protein of chicken lymphocytesurface molecule. Three mAbs recognized lymphocyte subpopulation in spleen and peripheral blood ofvillage chickens. In peripheral blood, mAbs BG4, CB1 and DB2 recognized lymphocytes subpopulationwith the percentages of 11.2%, 21.4% and 7.4% respectively. In spleen those three mAbs recognizedlymphocytes subpopulations at the percentages of 38.2%, 51.54% and 31.5% respectively. Based on thoseresult, it is very likely that mAbs BG4 and DB2 recognized CD4 molecule and mAb CB1 recognized CD8molecule of village chickens lymphocytes.
Hambatan Kolonisasi Methicillin Resistant Staphylococcus Aereus oleh Ekstrak Kulit Delima pada Luka Bakar Derajat-2 pada Tikus (INHIBITION OF METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS BACTERIA COLONIZATION ON SECOND DEGREE BURN WOUND IN WISTAR RAT BY US Iswinarno Doso Saputro; Lobredia Zarasade; Revita Widya Prasanti
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bacterial of Methicillin Resistant Staphylococcus Aureus (MRSA) is a pathogen that plays an importantrole as a nosocomial pathogen. The bacteria can cause outbreaks of nosocomial infection which is becomeserious problems in the management of burns patients because many strains have changed intomultiresistant bacteria to several classes of antibiotics. Treatment of MRSA bacterial infection in burnsis still a problem because its cost is relatively expensive and there is an increased trend of the bacteriaresistance. Therefore, alternatives are needed to deal with these problems. One of the alternatives is byusing topical pomegranate peel extract . This study was aimed to compare the topical administration ofpomegranate peel extract with mupirocin and to evaluate the combination effect of pomegranate peelextract with mupirocin against bacterial colonization of MRSA on the burn wound rat skin. Second degreeof burn was made by placing a nail with length 2 cm and width 2 cm. Before it used, the nail was boiled inboiling water for 10 seconds. Isolates of MRSA bacteria were inoculated on burn wound in male rats strainWistar. The rats were divided into three groups, each group was treated six hours after bacterial inoculation.The first group was given mupirocin on the burn wound; the second group was given topical pomegranatepeel extract, and the third group received a combination of mupirocin with pomegranate peel extracttopically. Swab culture was taken on the third and fifth day post-treatment. The specimen was sent to theMicrobiology Laboratory for microbiological examination to evaluate the efficacy of pomegranate peel extract in inhibiting bacterial colonization of MRSA. Topical pomegranate fruit peel extract significantlyinhibited MRSA bacterial colonization on the rat’s burn wound{p sig.(2-tailed),0,045, P < 0,05). Nosignificant result was found on bacterial count in topical pomegranate fruit peel extract administationcompared to topical mupirocin administration or in combination between topical pomegranate andmupirocin. Topical pomegranate fruit peel extract has antibacterial effect, eventhough it is not moreeffective compared to mupirocin administration or combination between the pomegranate and mupirocin.
Aktivasi Oosit Menggunakan Strontium Klorida setelah Injeksi dengan Spermatozoa Domba Hasil Pengeringbekuan (OOCYTE ACTIVATION USING STRONTIUM CHLORIDE FOLLOWING INJECTION OF FREEZE-DRIED RAM SPERMATOZOA) Takdir Saili; Ita Djuwita; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

One of the factors that inhibit the formation of male pronuclei following injection of freeze-dried ramspermatozoa was the absence of artificial activation during oocyte incubation after the injection. Therefore,in this experiment the ability of strontium chloride (SrCl2) to improve oocyte activation followingintracytoplasmic sperm injection (ICSI) was evaluated. Aceto lacmoid staining was used to assessdecondensation and pronucleus formation following ICSI. Results of this experiment revealed that freezedriedspermatozoa had the ability to decondense and to form 1PN following injection into oocytes evenwithout artificial activation, but failed to form 2PN. However, 40% of 2PN oocytes were obtained when theinjected oocytes was first incubated for 20 minutes in medium containing 50 mM strontium chloride thensubsequently incubated for 10 hours in medium without strontium. On the contrary, the 2PN oocytes werenot observed either in injected oocyte neither without artificial activation nor in non-injected oocytes withartificial activation. In conclusion, freeze-dried ram spermatozoa were able to decondense and to support2PN formation following ICSI and artificial activation using strontium.
Peran Transforming Growth Factor? terhadap Tingkat Kematangan dan Kejadian Apoptosis Oosit Sapi pada kultur In Vitro Widjiati -; Rimayanti -; Arief boediono; Agus Setiadi
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Low productivity of in vitro embryo production at blastosis level appears to be as a result of imperfectoocyte maturation which causes imperfect oocyte growth, and in turn will affect the embryo growth. Inaddition to hormonal factor, growth factor plays significant role in maturation process of oocytes. Asgrowth factor might has a significant role during maturation process, a study was conducted to determinewhether transforming growth factor ? (TGF ?) isolated from oocytes of cumulus complex is required for coculture and in vitro embryo production.. Oocytes were collected from follicles with the diameter of 3-5 mmand > 5 mm. Then the oocytes were cultured for 22 hours at 38.5o with 5% CO2 atmosphere in tissueculture medium (TCM) 199 supplemented with 5 ?g/mg luteonizing hormone (LH), 3% bovine serumalbumin (BSA) 50 ?g/ml gentamycin sulfat and three different levels of TGF ? (12,85 pg/ml, 25,7 pg/mland 38,55 pg/ml). The oocyte maturation and number of apoptosis cells were examined. The result showedthat oocyte maturation in medium supplemented TGF ? at the dose of 38,55 pg/ml was better than in thatat dose of 12,85pg/ml or 25,7 pg/ml. The best maturation was observed at metaphase II stage of oocytedevelopment. No apoptosis was observed during maturation of oocytes. Supplementation of TGF ? at thedose of 38,55 pg/ml in culture medium increased the oocyte maturation without causing a significantapoptosis in vitro.
Respons Metabolik terhadap Pembatasan Asupan Pakan pada Kambing Peranakan Ettawa (METABOLIC RESPONSES TO FOOD RESTRICTION IN ETTAWA CROSSBRED GOATS) Irkham Widiyono; Sarmin .; Bambang Suwignyo
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The study aim of this  was to evaluate the effects of food restriction on metabolic aspects (bloodchemistry parameters) in Ettawa crossbred goats. Four adult male and clinically healthy Ettawa crossbredgoats were used in this study. The animals were kept in individual cage for 12 weeks. Animal feed consistedof aerial part of peanut plant and concentrate at a ratio of 60:40. The experiment was devided into 2phases of feeding periods, fullfeeding (ad libitum  feeding and restricted feeding. After the adaptationperiod for 4 weeks (week 1-4), the animals were fed ad libitum for 4 weeks (week 5-8), and then continuedwith 50% of the ad libitum feed intake during the following 4 weeks restricted feeding period (week 9-12).During the experiment, drinking water was supplied ad libitum. Body weight were examined and bloodsamples were collected at the end of the fullfeeding and restricted feeding periods for blood chemistryanalyses. Statistical differences were determined by paired t-Test at probability level of 0.05. This studyshowed that feed restriction up to about 50% of ad libitum (80% of maintenance) feed intake resulted inchanges of blood chemistry parameters. At the end of restricted feeding period, the elevated concentrationof serum inorganic phosphate, creatinine, blood urea nitrogen, and cholesterine were observed and reducedconcentration of glucose, calcium, and triglyceride were observed. However no change was observed on  levelof arterial blood pH and the concentration of serum natrium, potassium, chloride, and protein. In conclusion,nutritional status has significant effect on blood chemistry parameters of Ettawa crossbred goats. Seruminorganic phosphate, calcium, creatinine, blood urea nitrogen, cholesterine, glucose, and triglycerideconcentration could provide the more reliable information for assessment of the nutritional status ofEttawa crossbred goat.

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