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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Respons Stres Sapi Bali Jantan pada Proses Pengangkutan dan Pemuatan ke Atas Kapal Ternak (STRESS RESPONSE OF BALI BULLS DURING TRANSPORTATION AND LOADING PROCCESS ONTO THE CATTLE SHIP) Sri Widayati; Puji Astuti; Irkham Widiyono
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.421 KB) | DOI: 10.19087/jveteriner.2019.20.4.566

Abstract

Transportation is needed for livestock distribution from production centers to consumption centers. This might potentially result in a stressful condition for the cattle. The study was aimed to observe the stress response of Bali bulls during the transport and loading proccess onto the ship. Six adult clinically healthy Bali bulls were used in this study. The animals were housed in pen at the animal quarantine installation, given dried straw and drinking water ad libitum since three days before the study. The animals were loaded on the truck in 30 minutes, transported to the harbor in 15 minutes, and loaded on to the ship in 30 minutes. On the ship the animals were kept in a pen bedded with straw and given dried straw and drinking water ad libitum for 19 hours. Venous blood samples were collected from jugular vein during the rest in pen before loading on to the truck, after loading on the ship, and about 19 hours after loading and resting in the ship pen. Serum was separated by centrifugation and stored at -20oC for cortisol analysis. The concentrations of cortisol was analyzed by using enzyme linked immunosorbent assay (ELISA). The results showed that the average of serum cortisol concentration of Bali bulls was at the level of 33.81±28.52 ng/mL in the rest period at the quarantine installation, increased significantly to the level of 138.41±102.57 ng/mL after loading onto the ship (P<0.05), and decreased again to the level of 41.54±43.47 ng/mL after 19 hours of resting period in the pen on the ship (P>0.05). It can be concluded that Bali bulls may suffer from stress during transport and loading proccess which can be recovered after resting in the ship pen.
TRAP PRODUCTION AND REDUCTION LARVAE III HAEMONCHUS CONTORTUS BY NEMATOPHAGOUS MOULDS Riza Zainuddin Ahmad
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was carried out to determine the ability of nematophagous moulds (Arthrobotrys oligosporaand Duddingtonia flagrans) to trap and reduce the number of H. contortus larvae III. Tests was conducted inpetri dishes containing agar medium, the moulds and Haemonchus contortus larvae. The ability of bothmould in trapping H. contortus larvae III was observed 3; 4; 10; 24 and 48 hours post-incubation, whereas theability of both mould in killing the larvae was observed 0,5; 3; 6; 9; 12; 15; 21; 24; 27 and 48 hours postincubation.The results showed that A. oligospora was more capable in trapping and reducing H. contortuslarvae III than Duddingtonia flagrans (p<0, 01). It was also evident that A. oligospora of Denmark originwas more capable in trapping and killing Haemonchus larve than that of local isolate. It is clear from thisstudy that A. oligospora is potential biological method for controling H. contortus infection in animals.
Resistensi Antibiotik pada Salmonella Isolat Sapi Bakalan Asal Australia yang Diimpor Melalui Pelabuhan Tanjung Priok Jakarta (ANTIBIOTIC RESISTANCE OF SALMONELLA ISOLATES FROM AUSTRALIAN IMPORTED FEEDER CATTLES THROUGH TANJUNG PRIOK PORT JAKARTA) Anindya Kurniawati; Denny Widaya Lukman; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.226 KB)

Abstract

Importation of feeder cattle from farms constantly using antibiotics in feed could result in the occurrenceof antibiotic resistant bacteria. A study was therefore conducted to investigate the possible use antibioticsand the antibiotic resistance of commensal Salmonella sppagainst several antibiotics. Salmonella spp.bacteria (n=50)were isolated from 100 samples of feeder cattlesfaeces. Total of 50 Salmonella spp. isolateswere subjected for Salmonella spp. examination and the isolated Salmonella spp. was tested for theantibiotic resistance using 10 antibiotics (ampicillin, cephalotin, erythromycin, tetracycline, streptomycin,chloramphenicol, trimethoprim, trimethoprim-sulfamethoxazole, nalidixic acid, and enrofloxacin) usingdisk diffusion method on Muller-Hinton agar following Clinical and Laboratory Standards Institute (CLSI)guidelines for interpretation. The isolated Salmonella showed resistance towards erythromycin 98%,ampicillin 34%, streptomycin 22%, nalidixic acid 8%, cephalotin 6%, tetracycline 4%, and chloramphenicol2%. There was no resistance against enrofloxacin, trimethoprim, and trimethoprim-sulfamethoxazole. Salmonella spp. bacteria derived from imported feeder cattle which have been resistant to antibiotics arepotential for spreading the antibiotic resistant bacteria to other susceptible animals. Such bacteria canalsotransfer the antibiotic resistant gene to other bacteria in Indonesia which ould be a potential threatfor public and animal healths.
Aktivitas Antagonis Bacillus subtilis terhadap Streptococcus iniae dan Pseudomonas fluorescens (ANTAGONIST ACTIVITY OF BACILLUS SUBTILIS AGAINST STREPTOCOCCUS INIAE AND PSEUDOMONAS FLUORESCENS) Budianto Budianto; Heny Suprastyani
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.135 KB) | DOI: 10.19087/jveteriner.2017.18.3.409

Abstract

Intensification of fish farming has caused various impacts, for example diseases in fish. One of the diseases in fish is a bacterial disease. The use of probiotic bacteria as an antimicrobial agent, which is relatively safe and effective, is a strategy to treat the disease. Bacillus subtilis is probiotic bacteria which can produce bacteriocin compounds and has antagonistic effects against both Gram negative and Gram positive bacteria. The aim of this study was to evaluate the antagonist activity of B. subtilis against Streptococcus iniae and Pseudomonas fluorescens. Antagonist activity test was done by using paper disc diffusion against the bacteria. The variations on the test paper disc method used were based on the difference of B. subtilis incubation time, such as: 0, 6, 12, 18, 24 and 30 hours. The results showed B. subtilis (generation time = 30.62 min) produced antibacterial compounds which inhibited the growth of the bacteria. Antagonist activity was detected in early exponential phase, six hours, with inhibition zone diameter of 7.28 ± 0.18 mm (S. iniae) and 6.75 ± 0.11 mm (P. fluorescens) and reached optimum at early stationary phase, 24 hours, the inhibition zone diameter of 8.84 ± 0.28 mm (S. iniae) and 9.14 ± 0.91 mm (P. fluorescens). It can be concluded that B. subtilis can contribute in controlling the spread of bacterial diseases in fish farming, particularly caused by S. iniae and P. fluorescens ABSTRAK Intensifikasi budidaya ikan telah menyebabkan berbagai dampak, seperti penyakit pada ikan. Salah satunya adalah penyakit bakteriawi. Penggunaan bakteri probiotik sebagai agen antimikrob yang aman dan efektif adalah salah satu strategi untuk menanggulangi penyakit tersebut. Bacillus subtilis merupakan bakteri probiotik yang dapat memproduksi senyawa bakteriosin dan memiliki efek antagonis terhadap bakteri Gram negatif dan positif. Tujuan dari penelitian ini adalah untuk mengevaluasi aktivitas antagonis dari B. subtilis terhadap Streptococcus iniae dan Pseudomonas fluorescens. Uji aktivitas antagonis dilakukan dengan menggunakan difusi cakram kertas terhadap bakteri uji. Variasi perlakuan pada uji cakram kertas adalah menggunakan perbedaan waktu inkubasi B. subtilis, yaitu waktu inkubasi 0, 6, 12, 18, 24 dan 30 jam. Berdasarkan hasil penelitian, B. subtilis (waktu generasi=30,62 menit) dapat memproduksi senyawa antibakteri yang dapat menghambat pertumbuhan bakteri uji. Aktivitas antagonis terdeteksi pada awal fase eksponensial yaitu inkubasi enam jam, dengan diameter zona hambat sebesar 7,28 ± 0,18 mm (S. iniae) dan 6,75 ± 0,11 mm (P. fluorescens) dan mencapai optimum pada awal fase stasioner yaitu inkubasi 24 jam, dengan diameter zona hambat sebesar 8,84 ± 0,28 mm (S. iniae) dan 9,14 ± 0,91 mm (P. fluorescens). Dapat disimpulkan bahwa B. subtilis dapat berperan dalam pengendalian penyebaran penyakit bakteri pada budidaya ikan, khususnya yang disebabkan oleh S. iniae dan P. fluorescens.
Anemia and Eosinophilia in Traditional Goat Farmers: Early Markers of Strongyle Zoonoses (ANEMIA DAN EOSINOFILIA PADA PETERNAK KAMBING TRADISIONAL: PENANDA AWAL ZOONOSIS STRONGYLUS) Insy Nafisah Taufik; Henhen Heryaman; Rini Widyastuti; Mas Rizky A. A. Syamsunarno; Mohammad Ghozali
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The spread of zoonoses between humans and animals can increase with the presence of specific contacts between the two. As well as intensive contact between farmer and goat. Anemia is a common hematologic change encountered in infection and zoonoses so it can be used as an early indicator for zoonoses. Nevertheless, anemia itself cannot lead to a specific etiology thus differential leukocyte count and goat fecal examination can be a good supporting test. Understanding the etiologies of the disease is critically important in preventing the decline in human’s quality of life as well as economic losses. This cross-sectional study included 30 farmers who were recruited by purposive sampling method. They agreed to do a CBC examination with 22 parameters. From the test, 37% of them showed mild anemia. There were 45% farmers with microcytic hypochromic anemia while the rest had normocytic normochromic anemia. There was an increased number in eosinophil and segmented neutrophil from anemic farmers, 18% and 9% respectively. Besides, 13% of goats physical examination indicated as below normal conditions characterized by lean bodies, lymph node swelling, and pale mucosa. Whereas 38% goat’s fecal test showed evidence of Strongyle eggs. The research suggests a possible interrelation between animals, humans, and the environment with an increased risk of zoonoses. Anemia and eosinophilia that occur can be an early marker which will be decisive not only for the success of the disease progression reduction but also the long term suppression of the economic loss.
Deteksi Gen Penyandi Resistensi ampC dan mcr-1 pada Escherichia coli penyebab Colibacillosis Unggas di Sukabumi (DETECTION OF GENE ENCODING RESISTANCE AMPC AND MCR-1 IN ESCHERICHIA COLI CAUSES AVIAN COLIBACILLOSIS IN SUKABUMI) Agustin Indrawati; Ryan Septa Kurnia; Ni Luh Putu Ika Mayasari
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.231 KB) | DOI: 10.19087/jveteriner.2019.20.4.495

Abstract

Antibiotic resistance has become a global problem that can threaten human and animal health. The use of antibiotics in livestock as a treatment and control of disease is often associated with the cause of the spread of resistant bacteria. Resistance bacteria are caused by presence of resistant gene resistance that can move between bacteria. This study aims to detect the presence of genes that encode resistance to ampicillin (ampC) antibiotics and colistin (mcr-1) in Escherichia coli bacteria derived from cases of colibacillosis in Sukabumi. A total of 25 isolates of E. coli archive collection of PT. Medika Animal Lab is used in this research. All isolates identified using PCR were then tested for sensitivity using the disk diffusion method and minimum inhibitory concentrations (MICs). Isolates that are resistant to ampicillin and colistin were tested for detection of ampC and mcr-1 genes using PCR. The results of the sensitivity test showed the whole isolates were resistant to ampicillin (100%) and phosphomycin (8%), but none were resistant to colistin sulphate. The total isolate E. coli successfully detected gene encoding resistance of ampC (100%). The results of sensitivity and resistance detection test showed that the whole isolates were ampicillin resistant and had the ampC resistance-encoding gene.
MINERALIZATION STUDY OF RENAL RATS FOLLOWING OVARYOHYSTERECTOMY AND ADMINISTRATION HIGH DOSE CALCIUM CARBONATE Wiwik Misaco Yuniarti; Ira Sari Yudaniayanti; Nusdianto Triakoso
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the effect of high dose calcium carbonate in rat (Rattus norvegicus) following ovaryohysterectomy. A total of twenty female rats at 13 week-old were used in this study. Following ovaryohitectomy, the animals were randomized in four treatment groups. Group P0 were :fed with standard food only P1, P2 and P2 groups treated with standard food but supplemented calcium carbonate respectively at the dose of 75 mg per animal per day, 225 mg per animal per day , and 450 mg per animal per day. The calcium carbonate supplement were given daily in the morning for 42 days. The experimental animals were sacrificed at 21 week-old. Calcium and phosphor level in sinister kidneys were determined by spectrofotometric method. The data obtained from this study were analysed using one way analysis of variance. No significant difference was observed in calcium level among four treatment groups, with the lowest level were found in P3 group. However, the phosphor level of P1 was significantly lower than those of P2 and P3 groups. The highest phosphor level was observed in P3 group, indicating a phosphorous retension and the signs of renal failure.
Ekstrak Sambiloto (Andrographis paniculata) Menurunkan Jumlah Skizon, Mikrogamet, Makrogamet, dan Oosista Eimeria tenella (EXTRACT OF ANDROGRAPHIS PANICULATA DECREASED SCHIZONTS, MICROGAMETES, MACROGAMETES AND OOCYSTS NUMBER OF EIMERIA TENELLA) UMI CAHYANINGSIH; RESSY RIANDCI; DYAH ISWANTINI
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to observe the effect of ethanol extract of Andrographis paniculata givenin grading doses to the schizonts, microgamete, macrogamete, and oocytes counts of Eimeria tenella inchicken caecum. A total of ninety day old broiler chicks were used in the study. At two weeks old the broilerswere divided into six groups. Each group consisted of 15 broilers, the 6 groups were: (i) negative control(broilers did not receive any treatment); (ii) positive control (each animal were infected with 104 E. tenellaoocytes); (iii) medicine control (each animal were infected with 104 E. tenella oocytes and coccidiostat); (iv)A1 (each animal were infected with 104 E. tenella oocytes and paniculata extract 90 mg/kg body weight); (v)A2 (each animal were infected with 104 E. tenella oocytes and paniculata extract 180 mg/kg body weight);and (vi) A3 (each animal were infected with 104 E. tenella oocytes and paniculata extract 360 mg/kg bodyweight). At day 6, 9, 13, 16, and 22 post infection three broilers from each group were sacrificed and theirceca were collected for histopathological examination. The results showed that paniculata extract at dose90 mg/kg body weight and 180 mg/kg body weight was able to decrease the numbers of shizont, microgamete,macrogamete, and oocytes of E. tenella in the chicken caecum.
Perkembangan Praimplantasi Embrio Mencit dengan Materi Genetik yang Berasal dari Parental, Maternal, dan Inti Sel Somatik (PRE-IMPLANTATION DEVELOPMENT OF MOUSE EMBRYO WITH GENETIC MATERIAL DERIVED FROM PARENTAL, MATERNAL AND SOMATIC CELL NUCLEUS) Harry Murti; Mokhamad Fahrudin; Mohamad Agus Setiadi; Boenjamin Setiawan; Arief Boediono
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.43 KB)

Abstract

Cloned embryo and parthenogenetic embryo are a potential source of stem cells for regenerativemedicine. Stem cells derived from those embryos are expected to overcome the ethical issues to the use offertilization embryos for therapeutic purposes. The pre-implantation development is a critical step fordeveloping embryos reach the blastocyst stage. The objectives in vivo of this research are to produce mousecloned embryo, parthenogenetic embryo, and fertilized embryo and to study stages of  in vitro pre-implantation development culture. In vivo fertilized embryos, mouse oocytes, and cumulus cells were usedin this study. Treatment was performed on female mice superovulated with PMSG and hCG injections.Two-cell stage of in vivo fertilized embryos were collected on the second day post hCG injection. Clonedembryos were produced through Somatic Cell Nuclear Transfer (SCNT), which included enucleation, nucleartransfer and artificial activation. Parthenogenetic embryos were produced with artificial activationtechnique. The result of the research indicated that SCNT application was able to produce cloned embryos which could develop to blastocyst stage (3,2%). In addition, artificial activation of oocytes could produceparthenogenetic embryos which were able to develop up to the blastocyst stage (8,6%). In conclusion,efficiency level of parthenogenetic embryos that is able to reach the blastocyst stage was higher than in thecloned embryos. Fertilized embryos shows a better development and more efficient compared to in vitrocloned embryos and parthenogenetic embryos cultures.
Viabilitas Spermatozoa Ayam Hutan Hijau dalam Pengencer Posfat Kuning Telur Ditambah Laktosa pada Penyimpanan 5oC (GREEN JUNGGLE FOWL SPERM VIABILITY IN EGG YOLK PHOSFAT DILUENTS CONTAINING LACTOSE STORAGED AT 5OC) Wayan Bebas; Desak Nyoman Dewi Indira Laksmi
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to determine the addition of lactose on egg yolk phosphate diluentsstored at 5°C to the plasma membrane intact, acrosome membranes intact of green jungle fowlspermatozoa, fertility and hatchability of chicken eggs. This study used completely randomized designwith three treatment groups. Treatment I (T0): egg yolk phosphate diluents only (control), treatment II(T1): egg yolk phosphate diluents + lactose 0.3%, and treatment III (T2): egg yolk phosphate diluents +lactose 0.6 %. Each group was replicated nine times. Semen was collected from eight green jungle fowl andevaluated both macroscopically and microscopically. Semen was diluted with each treatment with a singleinsemination dose (0.5 mL) with a concentration of 150 million progressively motile cells. Once diluted,semen was stored at 3-5°C for 48 hours and then was observed for the plasma membrane of intact,acrosome membrane of intact, and some semen were inseminated on local female chicken village forfertility, and hatchability eggs observation. The results showed that the addition of lactose 0.3% and 0.6%providing significant increase (P <0.05) on plasma membrane intact, acrosome membrane intact, fertilityand egg hatchability when compared to controls. However the addition of 0.3% and 0.6% lactose did notshowed a significant effect (P> 0.05). This study concluded that the addition of lactose on egg yolk phosphatediluents for storing green jungle fowl spermatozoa at 3-5oC for 48 hours can improve the plasma membraneintact, acrosome membranes intact, fertility and hatchability of chicken eggs.

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