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I G. Made Krisna Erawan
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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
Perbandingan Angka Fertilitas dan Hambatan Perkembangan Embrio Mencit yang Dikultur dalam Medium M16 dan Human Tubal Fluid (THE COMPARISON OF MICE FERTILITY RATE AND EMBRYONIC DEVELOPMENT CELL BLOCK WHEN CULTURED IN M16 AND HUMAN TUBAL FLUID MEDIA) Widjiati .; Sri Endah Pusporini; M. Zainal Arifin
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to compare the fertility rate and embryonic development cell block ofmice when cultured in M16 and Human Tubal Fluid (HTF) media, respectively. Two months old femaleBalbC mice were super ovulated using Pregnant Mare Serum Gonadotrophin (PMSG) and Human ChorionicGonadotrophin (HCG) prior to mating with vasectomies mice. At 17 hours post mating the mice wassacrificed for the collections of egg cells and spermatozoa. Egg cells were collected by tearing the fertilizationsac, while the sperm were collected from caudal epididymis. After the collection, both the egg cells andsperm were put in Petri dish containing M16 and HTF media and kept in 5% CO2 incubator at 370C for onehour prior to the in vitro fertilization (IVF) was performed. In vitro fertilization was performed in 5% CO2 incubator at 370C and kept for 24 hours in M16 and in HTF culture media. The results showed thatfertilization rate was 98.09% and 99.57%; cell block embryonic development was 85.09% and 83.36%when cultured in M16 and HTF media, respectively. In conclusion, HTF media can be used for culturingmouse embryo.
Preparasi Imunoglobulin G Kelinci sebagai Antigen Penginduksi Antibodi Spesifik Terhadap Virus Avian Influenza H5N1 Strain Legok Ketut Karuni Nyanakumari Natih; Retno Damayanti Soejoedono; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research was to prepare rabbit Immunoglobulin G as anti-idiotype antibody (Ab2) ofAvian Influenza Virus (AIV) H5N1. A polyclonal antibody was collected from guinea pigs immunized withinactivated AI vaccine H5N1of Legok strain. Antibody of H5N1 AI in serum was detected by Agar gelprecipitation test (AGPT) and an Inhibition Hemmaglutination test (IHT). The highest titre of antibodywas obtained one week after the third immunization. Serum of guinea pigs containing IgG was purifiedusing the Montage Antibody purification kit & spin column with Prosep A media (Millipore). The AI H5N1IgG concentration was 8 mg/ml. AI H5N1 IgG, was then digested with pepsin to obtain F(ab)2 fraction andwas called Ab1. The concentration of IgG and F(ab)2 and purity of IgG were determined by UVspectrophotometer which showed Ab1 concentration 1 mg/ml. Molecular weight was estimated by sodiumdodecyl sulfate- polyacrilamide gel electrophoresis (SDS-PAGE). Ab2 was produced by immunization ofrabbit with Ab1. The first immunization was carried out by subcutaneous injection with 500 ?g of Ab1emulsified in Complete Freund Adjuvant. The immunization was repeated with the same dose of Ab1emulsified in Incomplete Freund Adjuvan at 1 week intervals. One week after the second immunization,rabbit’s serum was harvested and IgG was purified using the Montage Antibody purification kit & spincolumn with Prosep A media (Millipore). The rabbit IgG, called Ab2, was an anti-idiotypic antibody againstAIV-H5N1. In AGPT, a precipitation line appeared between Ab1 and Ab2. A partial reaction appearedbetween Ab2 and the AI H5N1 antigen was also detected. The results indicated that Ab2 is a possiblecandidate of imunogen for protection against an AI virus H5N1 infection.
Enhancement of Nonspecific Immune Response and Growth Performance of Litopenaeus vannamei by Oral Administration of Nucleotides (PENINGKATAN RESPONS IMUN NONSPESIFIK DAN PERFORMA PERTUMBUHAN LITOPENAEUS VANNAMEI MELALUI PEMBERIAN NUKLEOTIDA SECARA ORAL) Henky Manoppo; Sukenda .
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research evaluated the nonspecific immune responseand growth of Litopenaeus vannamei fednucleotide diet.  In Laboratory, juveniles were reared in two groups of glass aquaria, each with threereplications.  Shrimps in group one were fed nucleotide diet and in group two were fedpellet four consecutiveweeks. Total Haemocyte Count and Phenoleoxydase activity were evaluated at the end of feeding whilegrowth was measured at two weeks interval. At the end of feeding, shrimps were intramuscularlyinjectedwith Vibrio harveyi  0.1x106 cfu.shrimp-1.  In tambak, juveniles were raised in two groups of net cages(hapa), each with three replications. One group was fed nucleotide diet while the other wasfed pellet forfour weeks. Total Haemocyte Count of shrimp fed nucleotide diet significantly increased up to 87% higherthan shrimps fed pellet.  Phenoleoxydase activity of shrimp fed nucleotides diet also increased isignificantlyas compared to shrimp fed pellet (p=0.02). Higher resistance and growth were observed in shrimp fednucleotide diet. In tambak, weight gain of shrimp fed nucleotide was 35.75% greater than shrimp fedpellet. Survival rate (83.24%) was higher than shrimp fed pellet (81.71%).  As conclusion, oral administrationof nucleotide at 400 mg.kg-1 diet could enhancethe nonspecific immune response, resistance, and growth ofL. vannamei.
Penurunan Kerusakan Jaringan Paru Terinfeksi Tuberkulosis oleh Ekstrak Pegagan Melalui Peningkatan Ekspresi Tissue Inhibitor of Matrix Metalloproteinase-1 (SUPLEMENTATION OF EFFECT ANILYSIS OF CENTELLA ASIATICA EXTRACT IN REDUCE LUNG TUBERCULOSIS TISSUE D Arifa Mustika; Anny Setijo Rahaju; Roostantia Indrawati
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Centella asiatica is a medicinal plant used for wound healing through increasing of collagen synthesis.This evidence generates a new expectation that it could be used for therapy of tuberculosis infection,especially for healing lung tissue damage. Until now, the effects and mechanisms onC. asiatica to cure thelung tissue damage due to M. tuberculosis infection remains unclear. The aim of this study was to prove theeffect and mechanism of ethanol extract of C. asiatica to repair the rats lung tissue damaged throughexpression of the enzimmatrix metalloproteinase-1(MMP-1)danenzimtissue inhibitor of matrixmetalloproteinase-1 (TIMP-1). The study was conducted in male rats. Twenty four rats were infected withM. tuberculosis through intratrachea and randomly divided into four groups. Group 1, 2 and 3 were thetreatment groups that they were given the ethanol extract of C. asiatica at dose 375mg/kgbw, 750 mg /kgbw, and 1500 mg / kgbw, orally and once a day for fourteen days. The fourth group was a control groupthat given distilled water. On day 15 rats were euthanized and lungs tissue have been taken. Evaluationof lungs tissue damage were assessed by the Dorman’s score in Hematoxylin Eosin and evaluation of the expression of MMP - 1 and TIMP 1 were performed by immunohistochemistry. Data of TIMP-1 wereanalyzed with ANOVA and data of lung tissue damage and MMP–1 were analyzed with Mann WhitneyU (á = 0.05). The results showed that there was a significant differences in the lungs tissue damagebetween the dose groups of 375 mg / kgbw and controls (p = 0.006), the dose groups at dose 750 mg / kgbwand controls (p = 0.004 ), the dose groups of 1500 mg / kgbw and controls (p = 0.043). There wasn’t asignificant difference between the treatment groups and control in the expression of MMP-1. In the expressionof TIMP – 1, there was a significant difference between the treatment group at dose of 750 mg / kg andcontrol. The conclusion of the study is the ethanol extract of C. asiatica has the ability to reduce lung tissuedamage of rats infected with M. tuberculosis, through increasesthe expression of TIMP-1
Performans Kerbau Lumpur dan Strategi Pengembangannya pada Daerah dengan Ketinggian Berbeda di Kabupaten Cianjur (PERFORMANCE ANALYSIS OF SWAMP BUFFALO AT DIFFERENT ALTITUDES IN CIANJUR DISTRICT AND ITS DEVELOPMENT STRATEGIES Komariah .; Cece Sumantri; Henny Nuraini; Sri Nurdiati; Sri Mulatsih
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research objectives were to analyze reproduction performance and productivity of swamp buffalofrom different altitudes in Cianjur and draw up a hierarchy of productivity strategy development usinganalysis of SWOT (Strength, Weakness, Opportunity, Threats) and Analytical Hierarchy Process (AHP)with four criteria: technology, costs, impact, and the response of farmers. Survey was conducted in Cianjurduring January-March 2014 by interview prepared questionnaires and direct observation of 63 buffalo farmers. Secondary data were also obtained from relevant agencies. Primary data were collected usingdirect observation of 139 reproductive female buffaloes then were further analyzed. A total of 58 buffaloesat their productive period were sampled and taken their morphometric data. Whilst 37 buffaloes weremeasured their frame size using Body Condition Score (BCS). The results showed that the reproductionperformance of buffaloes in the lowlands are not significantly different from those in the highland. The ageat first oestrus, first mating, first calving, gestation period were 25.6 months, 26.6 months, 38.7 months,11.8 months, respectively.. The oestrus period was 5.3 days, and post-partum mating interval was 54.6days. Differences in altitude and sex significantly affected (P <0.05) the morphometry assessment. Thebody weight of male buffaloes were found lower than the females both in highlands and lowlands (P<0.05).The body conditioning score of buffalo performance at highland was better compared to those in thelowland. Based on the SWOT analysis and AHP: (1) The main strategy is to improve the technology basedon the criteria of internal weakness by increasing scale holdings to seize opportunities buffalo meat selfsufficiency;(2) based on the criteria of cost and impact, the strategy was to cover threats over the professionout of the region by empowering farmers (facilitate increased productivity buffalo); (3) based on the responsecriteria, the primary strategy is to improve the quality of education of farmers by facilitating productivityimprovement opportunities to achieve self-sufficiency buffalo meat. The main development strategy basedon the four criteria: technology, cost, impact, and farmer response were increasing of buffalo ownershipscale, production facilities, and farmers education quality.
Pengaruh Pemberian Ekstrak Akar Alang-Alang terhadap Hati Mencit yang Menderita Gangguan Patologi (THE INFLUENCE OF THE ROOT OF SEDGE GRASS EXTRACTION ON LIVER OF MICES THAT TO SUFFER A PATHOLOGICAL DAMAGED) I Ketut Berata; Ida Bagus Oka Winaya
Jurnal Veteriner Vol 4 No 1 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Abstak dapat dibaca pada Full Text Abstract can be read at Full Text
Aluminosilikat Berpotensi Menekan Gangguan Reproduksi Mikotoksin Zearalenon Berdasarkan Pengamatan Jumlah Folikel dan Ekspresi Caspase-9 Ovarium Muhammad Thohawi Elziyad Purnama; Imam Mustofa; Tri Wahyu Suprayogi; Abdul Samik; Ragil Angga Prastiya; Amung Logam Saputro
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Zearalenone is a resorcylic acid lactone produced by fungal Fusarium graminearum in contaminated edible grains and can cause reproduction disorder in animals by binding to estrogen receptors on target cells. The aim of this study was to assess the potential use of aluminosilicates as mycotoxin binders to eliminate the adverse effect of zearalenone by examining the number of follicles and caspase-9 expression in the ovary of mice. The study adopted a completely randomized simple design using 20 mices which were randomly divided into five group each of which consisted of four mices. Five treatment groups consisted of K+ (without zearalenone and aluminosilicates); K- (treated with zearalenone 0.1 mg/mice/day); P1 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 0.5 mg/mice/day); P2 (treated with zearalenone 0.1 mg/mice/day and aluminosilicates 1 mg/mice/day); and P3 were treated with zearalenone 0,1 mg/mice/day and aluminosilicates 2 mg/mice/day) with gastric tube daily for 10 days. The data obtained from this study were analyzed by analysis of variance and proceeded with Duncan test. The result showed that the primary follicles, secondary follicles, tertiary follicles and de Graaf follicles increased significantly on P3 treatment group. Caspase-9 expressions decreased significantly in all of aluminosilicates groups as compared to positive control. The treatment of mice with zearalenone and aluminosilicates increases the number of follicles and decreased caspase-9 expression in the ovary of mice. ABSTRAK Zearalenon merupakan senyawa resorcylic acid lactone yang diproduksi oleh jamur Fusarium graminearum dan dapat mengakibatkan gangguan reproduksi pada ternak dengan membentuk ikatan pada reseptor estrogen. Penelitian ini bertujuan untuk menguji potensi aluminosilikat terhadap mencit yang telah dipapar zearalenon pada aspek jumlah folikel dan ekspresi caspase-9 organ ovarium. Penelitian ini menggunakan 20 ekor mencit yang dibagi menjadi lima kelompok perlakuan dengan masing-masing empat ulangan, yakni K+ tanpa dipapar zearalenon dan aluminosilikat; K- dosis zearalenon 0,1 mg/ekor/hari; P1 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 0,5 mg/ekor/hari; P2 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 1 mg/ekor/hari; P3 dosis zearalenon 0,1 mg/ekor/hari dan aluminosilikat 2 mg/ekor/hari dengan sonde lambung selama sepuluh hari. Data hasil skoring dan perhitungan dianalisis dengan sidik ragam dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa jumlah folikel primer, sekunder, tersier dan de Graaf pada P3 terjadi peningkatan yang signifikan. Ekspresi caspase-9 ovarium menunjukkan penurunan pada semua perlakuan aluminosilikat. Simpulan penelitian ini adalah mencit yang dipapar zearalenon dan kemudian diberi aluminosilikat mengalami peningkatan jumlah folikel dan penurunan ekspresi caspase-9 pada organ ovarium.
Analisis Isozim untuk Mengetahui Variasi Genetik Sebagai Upaya Pemurnian Ras Sapi Aceh Teuku Armansyah; Al-Azhar -; Tongku Nizwan Siregar
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was performed to investigate genetic variation of Aceh’s cattle based on analysis of malatedehydrogenase (MDH) and esterase isozymes. Blood samples were collected from 30 out of 345 Aceh’scattles raised at the Center for Breeding Prime Animals (BPTU) of Indrapuri, Aceh Besar. The phenotypecharacteristics of each cattle were recorded before blood collection, and then analyzed based on theRegulation of Agricultural Minister of Indonesia No. 54/permentan/ot.140/10/2006 whereas isozymesanalysis was done by vertical acrylamide gel electrophoresis under constant current of 10 mA at 4°C for 4hours and appropriate staining systems. The pattern band which showed the genotype of the testedcattles, were then used as the basis to determinate the heterozygosity and proportion of polymorphic locus.The results showed that phenotype variation was found among Aceh’s cattle raised at the BPTU Indrapuri,Aceh Besar. Two locus detected in this research were MDH 1 and MDH2, whereas esterase locus (Est1 andEst2) were not detected due to the unsuccesful of the electrophoresis. MDH1 and MDH2 loci in the redblood cells of Aceh’s cattle were polymorphic. On the basis of malate dehydrogenase isozymes usingMVSP 3.1 Program and Simple Matching Coefficient it could be concluded that there was individualgenetic variation among Aceh’s cattles raised at the BPTU Indrapuri, Aceh Besar.
Seleksi Spermatozoa Domba Garut dengan Metode Sentrifugasi Gradien Densitas Percoll Heri Sujoko; Mohamad Agus Setiadi; Arief Boediono
Jurnal Veteriner Vol 10 No 3 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Preparation of a good quality of sperm is required in order to increase the efficacy of in vitro fertilization.One method of a good sperm preparation is the use of Percoll density gradient centrifugation. This studywas aimed to determine the optimal combination of speed and time of centrifugation for the spermpreparation with the lowest mortality rate. Fresh semen collected from Garut sheep with the concentrationof 2 million cells per ml was used in this study. The experimental design used was a completely randomizedfactorial design with one control group and sixteen combination of speed and time of centrifugation. Theywere 1),(1) 200 xg for 5 min; (2) 200 xg for 10 min; (3) 200 xg for 15 min; (4) 200 xg for 20 min; (5) 300 xg for5 min; (6) 300 xg for 10 min; (7) 300 xg for 15 min; (8) 300 xg for 20 min; (9) 400 xg for 5 min; (10) 400 xg for10 min; (11) 400 xg for 15 min; (12) 400 xg for 20 min; (13) 500 xg for 5 min; (14) 500 xg for 10 min; (15) 500xg for 15 min; and (16) 500 xg for 20 min. All of control and treatments combination were replicated forthree times. Each control and combined treatment were replicated for three times. The variables observedwere the concentration of sperm, the percentage of live sperms, the percentage of progressively motilesperms and the percentage of normal sperm. The data obtained were analyzed by two way analysis ofvariance (ANOVA) and the differences between treatment groups were subjected for Duncan’s MultipleRange Test (DMRT). The best result was obtained using 400 xg for 15 minutes which showed the spermconcentration of 66.66 ± 12.22 millions per ml, the living sperms of 74.82± 1.53%, the percentage ofprogressively motile sperm of 57.56±8.42% and the percentage of normal sperm of 86±1.73%).
Faktor-Faktor Risiko Tertular Flu Burung di Desa-Desa Kabupaten Klungkung, Bali (THE RISK FACTOR OF BIRD FLU CASES IN VILLAGES IN KLUNGKUNG REGENCY, BALI) I Gusti Ngurah Badiwangsa Temaja; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was carried  out to identify the risk factor of  bird flu cases in villages in Klungkung regency,Bali. The data were collected through observation and interviews using questionnaire to head of householdsin villages of which were officially recorded as infected and uninfected area of bird flu.  Data was analyzedusing chi-square test and odds ratio to see the relationship AI infection and risks factors, as well as howsignificance is the relationship between them. The study showed that the crucial risk factor to bird flucases in infected villages were ceremonies (odds ratio : 2.401) and traditional rituals (odds ratio 3.229).The occurrence to bird flu in both villages is significantly affected by the following fctors: (1) poultry forconsuming, ceremony, and traditional ritual from markets and collected poultry, (2) disposal of deadchicken in to the  river, (3) the operation of villages markets, (4) Trading of live  poultry, (5) Commercialpoultry livestock in respected villages, (6) herding duck after rice harvesting, (7) frequency  of traditionalceremonies  in the villages.

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