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Jurnal Veteriner

jvet
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Published by Universitas Udayana

ISSN : 14118327 EISSN : 24775665 DOI : https://doi.org/10.19087/jveteriner

Core Subject : Health,

Veterinary

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki

Arjuna Subject : -

Articles 1,116 Documents

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Keragaman Genetik Kukang Jawa (Nycticebus javanicus) Menggunakan Control Region (D-loop) DNA Mitokondria (mtDNA) (GENETIC DIVERSITY ON JAVAN SLOW LORIS (NYCTICEBUS JAVANICUS) USING OF CONTROL REGION (D-LOOP) mtDNA) Wirdateti Wirdateti; Hayati Aziza; Handayani Handayani
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Javan slow loris (Nycticebus javanicus) one of the species of the genus Nycticebus is endemic in Java. Their distribution region is in West Java and Banten, and also reported to be found in Central Java and East Java, although very rarely. Status of the species is Critically Endangered due to high levels of poaching, habitat loss and habitat fragmentation, so that the necessary conservation in order to increase the population. For conservation management it is necessary to know the status of genetic resources that play of role in breeding, then this research is to explore the genetic population of the Javan slow loris from some locations in West Java. The research objective was to assess the diversity of their current population of Java loris through the control region (D-loop) of mitochondrial DNA (mtDNA). A total of 23 individuals samples from Gunung Halimun Park, Tasikmalaya, Garut, Ciamis, Jember and confiscated at the Rehabilitation Center IAR Bogor were used in this stydy. Specific primers of D-loop are used for loris with a length of 296 bp sequence. The result showed there are only five different sites and formed six haplotypes, each haplotypes only 1-3 nucleotides different. Low genetic diversity is shown as much as 42.96% of individuals show the same sequence or genetic distance (d) = 0 that indicate was monomorf population from different population. The genetic distance of the entire population was 0.003 ± 0:01 (0.3%).

Diagnosis Toksoplasmosis Kongenital Berdasarkan Gen Surface Antigen-1 Toxoplama gondii Isolat Lokal Menggunakan Polymerase Chain Reaction (DIAGNOSIS OF CONGENITAL TOXOPLASMOSIS BASED ON SURFACE ANTIGEN -1 GENE OF LOCAL ISOLATE TOXOPLASMA GONDII USING POLY Dwi Priyowidodo; Sri Hartati; Asmarani Kusumawati; Joko Prastowo
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Congenital toxoplasmosis has an important role in the transmission of toxoplasmosis in animals andhumans. Thus, a rapid and an accurate diagnostic method is needed. The aim of this study was to conductthe diagnosis technique of congenital toxoplasmosis in mice based on surface antigen-1 (SAG-1) gene oflocal isolates (IS-1) T. gondii using Polymerase Chain Reaction (PCR). A total of 15 pregnant mice Balb/C strain with the aged of eight weeks were used as experimental animal. Mice were intraperitoneallyinfected with 103tachizoit of T. gondii RH strain at day 9th of gestation. Amniotic fluids, blood, fetus, andplacenta then were collected at day 1, 2 , 3, 4 and 5 post infection. DNA was extracted from the abovesamples using PureLinkTM Genomic DNA Kit (Invitrogen, Life Technologies, US), and then amplified byusing specific primer based on SAG-1 gene of the local isolate T. gondii. This study shows that positivePCR result were seen in all samples of amniotic fluids at day 2 up to day 5 post infection. Fetus andplacenta samples also show positive PCR result at 3 up to day 5 post infection. Negative PCR result showsin blood samples, however. To conclude, PCR technique using SAG-1 gene of local isolates T. gondii as atarget gene, could be used to detect congenital toxoplasmosis from infected mouse samples such as, amnionfluids, fetus, and placenta. Further research was needed to apply the PCR method with SAG-1 gene of localisolate T. gondiion the human samples of congenital toxoplasmosis.

Deteksi Kerusakan DNA Spermatozoa Semen Segar dan Semen Beku Sapi Menggunakan Pewarnaan Toluidine Blue (DETECTION OF SPERM DNA DAMAGE INFRESH AND FROZEN SEMEN USING TOLUIDINE BLUE STAINING) Langgeng Priyanto; Raden Iis Arifiantini; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Sperm DNA integrity is very important in the success of fertilization and embryo development.Toluidine blue (TB) is a sensitive staining to examine the chromatin structure of sperm. The aim of thisstudy was to detect the sperm DNA damage before and after freezing using TB. Semen was obtained fromeight superior bulls (two Brahman, two Ongole, two Simental and two Limosin) belong to Lembang ArtificialInsemination Centre. Semen was collected twice a week using artificial vagina, then was evaluatedmacrocopically and microscopically after collected, including sperm motility, viability, plasma membraneintegrity (MI) and acrosome intact (AI), sperm concentration, abnormality and sperm DNA integrity. Thesemen that been used in this study showed the total motility was more than 70%, sperm concentrationwas more than 1000x106, and the sperm abnormality was below 20%. The result showed that the qualityof semen after freezing processed was decreased significantly (P<0.05) on percentage of sperm motility,viability, MI and AI, whereas there was no different on DNA integrity (P>0.05). Sperm DNA integrity offresh and frozen semen were 93.91±4.77% and 92.06 ±2.41% respectively. The decrease of DNA integritywas low (1.84%) compared to motility (28.3%), viability (21.6%), MI (14.1%), and AI (11.8%). In conclusion,toluidine blue can be used to detect of DNA damage and the freezing process will not decrease the DNAintegrity.

Pelacakan Protein Wingless-Type MMTV Integration Site Family Member-4 pada Uterus Mencit (DETECTION WINGLESS-TYPE MMTV INTEGRATION SITE FAMILY MEMBER-4 PROTEIN OF MOUSE UTERUS) Agung Janika Sitasiwi; Wayan Tunas Artama; Agung Budiyanto; Edy Dharmana
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aims of this study was to detect the expression of Wingless-type MMTV integration site familymember 4 (Wnt4) protein in the uterus of Swiss Webster mice. Laboratory animals that were used areadult Swiss Webster mice weighing 25-30 grams. Mice were kept and mated in a controlled laboratoryconditions. Pregnancy was determined by the presence of vaginal plug in female mice after breeding.Protein was isolated from the uterus at seven days of gestation. Immunoblotting was performed usingChemiluminescent Western Blot kit. The primary antibody used was anti Wnt-4 antibody with a 1: 1000dilution. The results showed protein bands with molecular weights ranging from 40 kDa showed a positivereaction to the primary antibody that were used so that it can be concluded that the protein is Wnt4protein.

Sediaan Daun Katuk dalam Pakan Ayam Pedaging Menurunkan Lemak Abdominal, Kadar Lemak, dan Kolesterol Daging (SUPLEMENTATION OF VARIOUS PREPARATIONS KATUK LEAVES IN FEED CAUSING A DECREASE OF ABDOMINAL FAT, FAT AND CHOLESTEROL LEVELS TO CARCASS OF BROILER Zulvia Maika Letis; Agik Suprayogi; Damiana Rita Ekastuti
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This research was designed to determine the efficacy of various preparations of katuk (Sauropus androgynus L.) leaves on productivity and carcass quality of broiler chiken. This research used experimental methods of block randomized design. Experimental animals used in this study were 100 male broiler chickens (Ross) with body weight ±40 g. The chickens were divided into five experimental groups, namely: control group (control), katuk leaves powder (TDK), extract of dried katuk (EKK), extract of brewed katuk (EKS) and katuk juice (KP). Each treatment consisted of twenty chickens. The administration of feed treatment every day from the seventh days to 37th days-old chicken (age 1th to 5th) ad libitum. The results showed that the administration of preparation extract and juice (EKK, EKS and KP) leaves katuk able to improve carcass quality and prevent the decline in productivity growth in body weight compare with leaf meal preparation katuk (TDK) real body weight can reduce the growth of broiler chickens is 1645,80 g/individual (EKK), 1450,30 g/individual (EKS), 1472,50 g/individual (KP) and 1408,90 g/individual (TDK). The administration of various preparations of katuk had a positive response, especially in improving carcass quality characterized by the decrease in abdominal fat deposits, level of fat and cholesterol broiler meat. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh berbagai sediaaan katuk (Sauropus androgynus L.) terhadap produktivitas dan kualitas daging ayam pedaging/broiler. Penelitian ini menggunakan rancangan acak kelompok (RAK). Hewan coba yang digunakan dalam penelitian ini sebanyak 100 ekor ayam broiler jantan (strain Ross) bobot badan sekitar 40 g yang dikelompokan ke dalam lima kelompok perlakuan pakan, yaitu: kontrol, tepung daun katuk (TDK), ekstrak katuk kering (EKK), ekstrak katuk seduh (EKS), dan katuk perasan (KP). Setiap perlakuan terdiri atas 20 ekor ayam. Pemberian pakan perlakuan dilakukan setiap hari mulai usia hari ke tujuh sampai ayam berusia 37 hari(minggu ke-1 sampai mingu ke-5) ad libitum. Hasil penelitian menunjukkan bahwa pemberian sediaan ekstrak dan perasan (EKK, EKS, dan KP) daun katuk mampu meningkatkan kualitas karkas dan mencegah penurunan produktivitas pertumbuhan bobot badan dibandingkan dengan sediaan tepung daun katuk (TDK) yang nyata dapat menurunkan pertumbuhan bobot badan ayam broiler yaitu 1645,80 g/ekor (EKK), 1450,30 g/ekor (EKS), 1472,50 g/ekor (KP), dan 1408,90 g/ekor (TDK). Pemberian berbagai sediaan katuk memiliki respons positif terutama dalam peningkatan kualitas karkas ditandai dengan penurunan deposit lemak abdominal, kadar lemak, dan kolesterol daging ayam broiler.

Radiographic Evaluation of Rabbit Femur Implanted Bali Cattle Bone Graft I Wayan Wirata; Steven Dwi Purbantoro; Luh Made Sudimartini; IWN Fajar Gunawan; AA Oka Dharmayudha; IGAG Putra Pemayun
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Bone xenograft from cattle bone is commonly used to treat a comminuted fracture case. This study aims to know the process of fractured-femur bone healing in rabbit post-implantation powder bone graft from cortical femur bone of Bali cattle evaluated by radiographs. Ten male local rabbits were used in this study, which were divided into 2 groups randomly. Group I (KI) as control, the diaphysis of femur bone was drilled with a diameter of 5 mm without implanting the bone graft, while Group II (KII), the diaphysis of femur bone was drilled 2 holes with a diameter of 5 mm each and with distance 20 mm, substituted with mineralized powder bone graft for the proximal hole (KIIa) and demineralized powder bone graft for the distal hole (KIIb). Fracture healing evaluation was done at week 0 (24 hours), 2, 4, and 6 postoperative by monitoring the growth of callus, fracture line, and union process with radiograph based classification according to Hammer et al., tabulated statistically, and presented descriptively. The results showed that KI and KII were in the sequel of fracture healing but had not reached remodeling phase perfectly. In conclusion, mineralized and demineralized powder bone graft used in this study was as osteoconductive and the use of bone graft shows no different significance and time shows different significance to fracture healing.

Karakteristik Fisik dan Nilai Organoleptik Sosis Daging Kuda Berdasarkan Level Subtitusi Tepung Tapioka (PHYSICAL CHARACTERISTICS AND ORGANOLEPTIC VALUESOF HORSE’S MEAT SAUSAGE BASED ON LEVEL SUBTITUTION OF TAPIOCA FLOUR) Bulkaini Bulkaini; Djoko Kisworo; Muhammad Yasin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of the study was to determine the effect of the tapioca flour level substitution on physical characteristics and organoleptic values of sausage made of male horse’s meat.The material used was meat of 4.0-4.5 years old male horse and a number of spices in making sausages. This study was conducted in a One Way Completely Randomized Design with four treatments and three replications, namely P1 = Control (without tapioca flour); P2 = 15% tapioca flour; P3 = 30% tapioca flour and P4 = 45% tapioca flour. The data obtained were analyzed using analysis of variance. The treatments which significantly different were then continued analyzed using the Duncan New Multiple Range Test. The results showed that the level of tapioca flour substitution had a significant effect (P <0.05) on water holding capacity (WHC), color, aroma and texture of the sausage, while the cooking lost, tensile strength, pH, and taste were not affected significantly (P> 0.05). The substitution of 30% tapioca flour from the ingedients gave the highest value of horse’s meat sausages in WHC of (45.74 ± 8.18) %, but low in cooking losses of (3.33 ± 1.15) %, low tensile strength of (0.45 ± 0.00) kg/cm2 and normal pH (6.66 ± 0.05). Based on organoleptic values that substitution of 30% tapioca flour gave slightly brown color of sausage (3.83 ± 0.03), good aroma (5.95 ± 0.04), delicious taste (6.20 ± 0.03) and soft texture (5.68 ± 0.02).

Bakteri Legionella pneumophila Terdeteksi pada Air Kolam Renang di Kota Surabaya dengan Nested Polymerase Chain Reaction (LEGIONELLA PNEUMOPHILA BACTERIADETECTED IN SWIMMING POOL WATER OF SURABAYA BY USING NESTED POLYMERASE CHAIN REACTION) Eduardus Bimo Aksono; Ana Adelina Farahdiba; Eka Pramyrtha Hestianah
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Legionella pneumophila is a Gram-negative bacillus that causes nosocomial and community-acquired pneumonia. The aim of this research was to detect the presence of bacteria of L. pneumophila species in the swimming pools water of Surabaya city by using nested Polymerase Chain Reaction (PCR) assay of a specific gene for L. pneumophila (mip gene). This study used purposive sampling method. A total of 10 water samples were collected from five swimming pools consisting of 200 mL water for each swimming pool. The results showed that of 10 samples tested by nested PCR, one sample was positive for L. pneumophila, and nine samples were negative. L. pneumophila were found in pool water samples with a higher temperature (>30ºC).Serogrouping analysis of positive sample that L. pneumophila bacteria detected in the water sample of swimming pool in Surabaya was L. pneumophila serogroup 9 (98%) and serogroup 10 (98%). L. pneumophila detection of bacteria is expected to raise the awareness of physician and microbiologists about the transmission of L. pneumophila and will also be useful for controlling the agents. ABSTRAK Legionella pneumophila adalah bakteri Gram-negatif berbentuk batang yang dapat menyebabkan penyakit nosokomial dan pneumonia. Tujuan penelitian ini adalah untuk mendeteksi keberadaan bakteri L. pneumophila pada air kolam renang di Kota Surabaya dengan menggunakan nested Polymerase Chain Reaction (PCR) berbasis gen spesifik L. pneumophila (mip gene). Penelitian ini menggunakan metode purposive sampling. Sebanyak sepuluh sampel diambil dari lima kolam renang. Sampel diambil sebanyak 200 mL dari air kolam renang di setiap lokasi. Hasil dari 10 sampel yang diuji menggunakan nested PCR, satu sampel menunjukkan hasil positif untuk L.pneumophila, dan sembilan sampel menunjukkan hasil negatif. Bakteri L. pneumophila ditemukan pada sampel air kolam dengan suhu yang lebih tinggi (>30ºC). Satu sampel positip tersebut ketika dilanjutkan terhadap analisis serogrup terlihat bahwa bakteri L. pneumophila yang terdeteksi pada air kolam renang di Kota Surabaya termasuk L. Pneumophila serogrup 9 (98%) dan serogrup 10 (98%). Terdeteksinya bakteri L. pneumophila ini diharapkan dapat meningkatkan kesadaran dokter dan ahli mikrobiologi tentang penyebaran L. pneumophila dan juga bermanfaat untuk mengontrol agen Legionellosis.

Teknik Penanganan Kendali Hewan Sesuai Kaidah Kesejahteraan Hewan Meningkatkan Akurasi Pengukuran Profil Hemodinamika Tikus Laboratorium Huda Shalahudin Darusman; Setyo Widi Nugroho; Fauzi Abdurrahman Munggaran; Dondin Sajuthi
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Chronic discomfort potentially occurred during a hemodynamic measurements in a rat-tail blood pressure chamber due to the animal is restrained in restricted space for a long period – it took 10 repetitions each at approximately 1.5 minutes for a session of hemodynamic measure. Procedures related to the animal welfare methods are required to improve the accuracy of the measurement which would altered from the discomfort to comfort state of the animal. Welfare procedures are represented by humane handling and habituation of the subject (rats) to the operator and the hemodynamic apparatus. Seven rats were subjected in this study and measured for hemodynamic value in three times point: before (T0), after humane handling (T1) and after human handling and habituation to the measurement apparatus (T2), respectively. Six out of seven subjects showed a significant lowering deviation for all variables of hemodynamic measurements, and that deviation followed by a declining trend from T0 to T2, i.e T0>T1>T2. The results indicated that welfare-based approach would benefit the animals from feeling discomfort and yet improving the accuracy and quality of the outcomes.

Perkembangan Histologis Ovarium Bayi dan Anak Owa Jawa (Hylobates moloch) (HISTOLOGICAL DEVELOPMENT OF NEONATE AND JUVENILE JAVAN GIBBON (Hylobates moloch) OVARIES) Pristiani Nurantika Notosoediro; Adi Winarto; Ligaya Innocentia Theresia Antoinetta Tumbelaka
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Reproductive success is one of the biggest challenges for the existence of javan gibbon (Hylobates moloch) in the future. Basic biology information of main reproduction female organ of the species is yet unknown. The research aimed to provide information of female ovary development through histological examination. Two pairs of ovaries were collected from a neonate and a three years old female cadaver at Javan Gibbon Center. Histological techniques (cross and longitudinal sections) were applied to the collected samples using paraffin method with haematoxylin eosin (HE) and Masson’s trichrome (MT) dyes. The follicles are spread evenly in the left and right cortex ovary. The number of primordial follicles within the neonate and infant ovary was 80 815 and 34 622, respectively. In a three years old javan gibbon ovaries, the development of primordial into primary, pre-antral and antral follicles were observed. The average diameter of the follicles and oocytes were, respectively; 25.0±8.9 ?m and 20.0±13.1 ?m for primordial follicle, 48.4±22.5 ?m and 20.5±10.0 ?m for primary follicle, 79.0±49.0 ?m and 25.4±17.4 ?m for pre-antral follicle, 315.5±36.1 ?m and 32.0±17.0 ?m for antral follicle. The size of primordial and primary oocyte and follicle of javan gibbon is smaller than those of Macaca fascicularis at the same age. The connective tissue of neonate ovary was being developed while in the 3 years old female ovary, it was well-developed and clearly seen in the capsula, cortex, and medulla. Javan gibbon follicle development is strongly influenced by age.

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I G. Made Krisna Erawan

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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Editorial Address Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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I G. Made Krisna Erawan

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krisnaerawan@unud.ac.id

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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Kota denpasar,

Bali

INDONESIA