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Jurnal Veteriner

jvet
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Published by Universitas Udayana

ISSN : 14118327 EISSN : 24775665 DOI : https://doi.org/10.19087/jveteriner

Core Subject : Health,

Veterinary

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki

Arjuna Subject : -

Articles 1,116 Documents

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Infeksi Alami Canine Parvovirus pada Anjing Kintamani di Desa Sukawana, Kintamani, Bangli, Bali (NATURAL INFECTION OF CANINE PARVOVIRUS IN KINTAMANI DOGS OF SUKAWANA VILLAGE, KINTAMANI, BANGLI, BALI) I Gusti Ayu Agung Suartini; Indrawati Sendow; I Nyoman Suarsana; Ni Luh Eka Setiasih; Maratun Janah
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Kintamani dog as one of germ plasm owned by Bali province has been widely accepted as dog of Indonesian origin which need to be preserved. Report have shown that puppies of Kintamani dogs sold in Denpasar animal market often die due to Canine parvovirus (CPV) infection. The mortality of CPV infection in puppies can reach as high as 91% espescially in unvaccinated dogs. As the mortality of CPV in dogs is very high, it is important to find out the seroprevalence of CPV infection in Kintamani dogs in Sukawana village. Up to now, the seroprevalence of CPV infection in Sukawana, the natural habitate of Kintamani dog has never been reported. In this study the sample collection and area selection was conducted by haemaggutination inhibition (HI) test. Sera sample were concluded positive if the HI titers of sera were > 64 HI units. Seroprevalence of CPV infection was calculated by dividing the number of positive sera with the total sera samples. The seroprevalence of CPV among dogs was determined using non parametric analysis (Chi-Square). From 70 sera samples collected 67.1% (47/70) were antibody positive against CPV. The highest seroprevalence was found in Banjar Sukawana 22.8% (16/70). A higher seroprevalence was found in female dogs 45.7% (32/70) compare to male dogs 21.4% (15/70). Kintamani dogs aged between 724 month have the highest seroprevalence 27.1% (19/70). Based on the distribution of antibody titers, the seroprevalence antibody >64 HI was 65.7%. The result showed that the high titer (> 64 HI) of antibody against CPV, it was shown that CPV infection has occurs naturally in kintamani dog at Sukawana village.

Keragaman Jenis dan Prevalensi Lalat Pasar Tradisional di Kota Bogor (DIVERSITY AND PREVALENCE OF FLIES AT TRADITIONAL MARKETS IN BOGOR CITY) Puguh Wahyudi; Susi Soviana; Upik Kesumawati Hadi
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.155 KB)

Bogor city is one of the greater Jabodetabek area which has a fairly high growth of the modern market.This should not shift the role of traditional market, if accompanied with an increase in the number andquality of traditional markets, among others by controlling infestations of flies on the market that can bea vector of various diseases.This research was conducted to identify the diversity and infestation of fliesspesies in five old Bogor traditional markets. The flies were collected using insect nets and then killed withchloroform to count and identification purposes. Measuring the prevalence of flies infestation in eachmarket were using sticky fly paper on block sale of meat, fish and outside market environment. Therewere ten fly spesies belong to four main families that Calliphoridae (C. megacephala, C. saffranea, C.rufifacies, and Lucilia sericata), Muscidae (M. domestica, M. conducens, and M. fasciata), Sarcophagidae (S.haemorroidalis, and S. fuscicauda), and Drosophilidae (Drosophila repleta). The others three families werePhoridae, Anthomyiidae, and Syrphidae. Fly diversity index on each markets were 1.203 (Bogor Market),1.038 (Sukasari Market), 2.678 (Anyar Market), 1.017 (Jambu Dua Market), and 1.618 (Gunung BatuMarket). Measurement of Calliphorid flies infestations as an indicator of the presence of litterdecomposition of organic material showed a high concentration in the market environment.These resultsillustrate the general environmental sanitation of traditional markets are bad.

Serbuk Daun Jambu Biji Memperbaiki Performans Pertumbuhan dan Morfologi Duodenum Ayam Jawa Super (GUAVA LEAF POWDER IMPROVING PERFORMANCE OF GROWTH AND DUODENUM MORPHOLOGY OF JAWA SUPER CHICKEN) Haris Setiawan; Listiatie Budi Utami; Muhammad Zulfikar
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Natural bioactive compounds used as feed supplements in feed could increase chicken productivity in the livestock industry. The research aims to study the use of guava leaf powder as a source of bioactive compounds in the feed on growth performance and duodenum morphology of Jawa Super Chicken. This study used 80 days old chick (DOC) Jawa Super chickens reared until the age of 16 days. The study used a completely randomized design, with the addition of treatments guava leaf powder in the basal feed from 0 g/kg diet (control); 2.5 g/kg of feed (P1); 5 g/kg of feed (P2); 10 g/kg of feed (P3). Parameters observed included growth performance, body components, digestive organ components and duodenum morphology consisting of crypt depth, height, and area of villi. Data was analyzed by using one-way analysis of variance then continued with LSD and Duncan test (Pd”0.05). The results showed that guava leaf powder could significantly increase the height of villi, and components of digestive organs in chickens. The results of the duodenum morphology observation showed that the P2 and P3 are better than the control (Pd”0.05). The growth performance and body components of the Jawa Super chicken did not show a significant difference (Pd”0.05) between the control and the treatment. In conclusion, the guava leaf powder with a dose of 10 g/kg body weight can increase duodenal villi growth, the weight of intestine and liver without influence the weight of Jawa Super chickens aged 16 days.

Identifikasi Keragaman Genetik Gen 12S Ribomsom RNA Sebagai Penanda Genetik untuk Penentuan Spesies Kuskus (IDENTIFICATION OF GENETIC DIVERSITY 12SRRNA GENES AS GENETIC MARKER FOR DETERMINING SPECIES CUSCUS) Rini Widayanti; Hery Wijayanto; Woro Danur Wendo; Rony Marsyal Kunda
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (246.9 KB)

Cuscus is marsupial’s animal (Phalageridae) which has limited spread in eastern Indonesia (Sulawesi,Maluku, Papua), Australia and Papua New Guinea. The ex-situ and in-situ conservation of cuscus undercaptivating condition is an alternative solution to protect cuscus from extinction. This study aimed todetermine nucleotide sequence and genetic markers on 12Sr RNA gene with sequencing method of eachspecies on three islands. Whole genome DNA was extracted from 17 samples of cuscus obtained fromdifferent habitats, Sulawesi (2 individual), Maluku (7 individual), and Papua (8 individual) according tothe protocol of Qiamp DNA Blood Mini Kit (Qiagen), and then it was used as template for amplificationof 12Sr RNA gene by using PCR. PCR product were then purified using column chromatography and wereused as template for sequencing reaction. Result sequencing of 12Sr RNA gene were analyzed usingMEGA program version 6. PCR product gives a result nucleotida of 958 bp according to databasegenebank, sequencing product gives result nucleotida of 896 bp and found of 105 different nucleotide sites.Filogram based on nucleotide sequences 12SrRNA gene from Sulawesi cuscus is Ailurops ursinus whereasthe cuscus from Papua and Maluku is Phalanger sp. and Spilocuscus maculatus species. Thirteen nucleotidasites were found, sites no 67 (A/G), 89 (G/C), 137 (T/C), 285 (G/A), 468 (T/C), 595 (T/C, 598 (T/C), 647 (T/C),654 (G/A), 665 (T/C), 769 (C/T), 874 (C/T), and 876 (A/G) which can be used as genetic marker betweenPhalanger genera from Papua and Maluku, and three nucleotida sites (sites no 127 (G/A), 481 (C/T), and885 (T/C) can be used as genetic marker between Spilocuscus genera from Papua and Maluku.

Pengetahuan, Sikap, dan Praktik Pemilik Breeding Kennel terhadap Pencegahan dan Pengendalian Bruselosis pada Anjing Impor (KNOWLEDGE, ATTITUDE, AND PRACTISES OF BREEDING KENNEL OWNER REGARDING CANINE BRUCELLOSIS PREVENTION AND CONTROLLING ON IMPORTED DOGS Citra Noviana; I Wayan Teguh Wibawan; Etih Sudarnika
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.374 KB)

This study was conducted to determine the seroprevalence of Brucella canis of imported dog for breederthrough Soekarno Hatta International Airport, Cengkareng Tanggerang, Indonesia. A total of 64 serumsamples were obtained from breeder dogs imported to Indonesia between Mei–September 2014. Theserums were examined by Immunochromatographic Assay. None of the serum samples in this studytested positive. Result of this study indicated that antibodies of B. canis were not detected in 64 dogsimported to Indonesia during the research period. A Knowledge, Attitude and Practises (KAP) Survey wasalso performed. This study used 32 respondents and data were analyzed by using path analysis. Therespondents were breeding kennel owners (breeder) who imported dogs through Soekarno HattaInternational Airport during the research period. The study concluded that the knowledge significantlyinfluenced the attitude of the breeder, and also the attitude significantly influenced the practises ofpreventing and controlling brucellosis. The annually income was also identified as a variable thatsignificantly influenced the practises. Practice prevention and control of canine brucellosis can be improvedby increasing the knowledge of the breeding kennel owner.

Physical Properties of Cervical Mucus of Repeat Breeder Aceh Cattle Tongku Nizwan Siregar; Iin Agustina; Dian Masyitah; Al Azhar; Dasrul Dasrul; Cut Nila Thasmi; Rusli Sulaiman; Razali Daud
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study aimed to investigate the physical property changes of cervical mucus in repeated breeder Aceh cows. Six cows consisting three normal (K1) and three repeat breeders (RB) (K2) of adult Aceh cows were used. The characteristics of K1 group were at > 2 months post parturition, successful pregnancy following once insemination, and twice regular estrus cycle twice. The K2 were cows with normal estrus but failed to be pregnant after three times artificial inseminations. Cervical mucus samples (50 ml each) were collected by aspiration using a sterile catheter and disposable syringe. The comparisons of the physical properties of cervical mucus between fertile and repeat breeder cattle were as follows.. Cervical mucus unavailability/in small quantity was 0.00 vs. 66.67%, cloudy color was 0.00 vs. 66.67%, thick consistency was 0.00 vs. 100%, fern pattern was 0.00 vs 66.67%, spinnbarkeit was 5.16±1.60 vs. 2.83±2.02 cm and pH values was 7.33±0.57 vs 9.33±1.52. in conclusion, the physical properties of cervical mucus in repeat breeder Aceh were less in quantities, more cloudy, higher in viscosity, and higher pH as compared to normal fertile cows. ABSTRAK Penelitian ini bertujuan mengetahui perubahan sifat fisik mukus serviks sapi aceh yang mengalami kawin berulang (repeat breeding, RB). Dalam penelitian ini digunakan enam ekor sapi aceh betina yang terdiri dari tiga ekor sapi normal (K1) dan tiga ekor sapi yang mengalami RB (K2). Sapi K1 merupakan sapi >2 bulan pascapartus yang mempunyai riwayat berhasil bunting dengan sekali inseminasi dan mempunyai dua kali siklus reguler, sedangkan K2 terdiri atas sapi yang didiagnosis mengalami RB, yaitu sapi yang gagal bunting setelah lebih dari tiga kali inseminasi namun memiliki siklus estrus normal. Sampel mukus serviks dikoleksi dengan metode aspirasi menggunakan kateter steril dan disposible syringe 50 mL. Kateter dimasukan melalui vagina yang yang diiringi dengan palpasi rektal untuk mengarahkan kateter masuk ke serviks uterus. Hasil pemeriksaan sifat fisik mukus serviks pada sapi fertil vs sapi RB menunjukkan bahwa kuantitas mukus tidak ada/sedikit (0,00 vs 66,67%), warna keruh (0,00 vs 66,67%), konsistensi kental (0,00 vs 100%), pola pakis (0,00 vs 66,67%), spinnbarkeit (5,16±1,60 vs 2,83±2,02 cm) dan nilai pH (7,33±0,57 vs 9,33±1,52). Dari hasil penelitian dapat disimpulkan bahwa sapi aceh yang mengalami RB memiliki kuantitas mukus lebih sedikit, warna lebih keruh, konsistensi kental, dan pH yang lebih tinggi dibanding sapi aceh yang fertil.

Pengembangan Teknologi Mikroenkapsulasi Bakteri Probiotik dan Manfaatnya untuk Kesehatan (TECHNOLOGY DEVELOPMENT OF PROBIOTIC BACTERIA MICROENCAPSULATION AND IT BENEFIT FOR HEALTHY) Raden Haryo Bimo Setiarto; Harsi Dewantari Kusumaningrum; Betty Sri Laksmi Jenie; Tatik Khusniati
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Probiotic based products are associated with many health benefits. However, the main problem is the low survival of these probiotic in food products as well as in gastrointestinal tract. Providing probiotics with a physical barrier is an efficient approach to maintain microorganisms and to deliver them into the gut. Microencapsulation is one of the most efficient methods, and it has been under consideration and investigation by some researcher. Generally, the success of this technology is depend on the quality of the wall material, probiotic strain, the core release form and the encapsulation method. Therefore, in this review, some relevant microencapsulation aspects, such as the capsule, wall material, core release forms, encapsulation methods will be briefly discussed. In this sense, microencapsulation has gained an increasing interest, since it has been demonstrated that it could protect the destruction of bacteria not only during its production process but also during its delivery into gastrointestinal tract, as well as it function as a protective effects during storage.

Kaji Banding Kualitas Semen Segar Empat Genetik Ayam Lokal Indonesia (COMPARATIVE STUDY ON THE QUALITY OF FRESH SEMEN OF FOUR GENETIC LOCAL CHICKEN IN INDONESIA) Junaedi Junaedi; Husnaeni Husnaeni
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was conducted to examine the comparison the quality of spermatozoa in four genetic groups of poultry (Pelung chicken, Nunukan chicken, Sentul chicken, and Bangkok chicken). The parameters of this study were the concentration of spermatozoa, motility, viability, semen volume, color, and consistency. The study was designed using a Completely Randomized Design (CRD) with four local chicken nations and four replications. The results showed that the volume of Nunukan chicken semen was 0.32±0.01 mL/ejaculation, Bangkok chicken was 0.31±0.01 mL/ejaculate, Sentul chicken was 0.15±0.02 mL/ejaculate and Pelung chicken was 0.23±0.02 mL/ejaculation. the color of fresh semen is white (Pelung chicken, Nunukan chicken and Sentul chicken) except chicken Bangkok has the color of cream semen. Pelung chicken has a concentration of spermatozoa 5,043.33±51 million/mL, Nunukan chicken 3,250.22 ±45 million/mL, Sentul chicken 3,002.87±67 million/mL, and Bangkok chicken 3,002.87 ± 67 million/mL. Motility of Pelung chicken 84.,69 ± 1.12%, Bangkok chicken 82.35±1.85%, Nunukan chicken 77.74±1.57% and Sentul chicken 77.64±1.65%. Viability spermatozoa of Sentul chicken was 90.35 ± 1.21%, Bangkok chicken was 90.64 ± 1.16%, Pelung chicken was 89.17 ± 1.23% and Nunukan chicken was 86.29 ± 1.15%. It can be concluded that the motility spermatozoa of Pelung chicken and Bangkok chicken is better than the spermatozoa motility of Nunukan chicken and Sentul chicken. The viability of Sentul chicken and Bangkok chicken was higher compared to the viability semen of chicken Pelung and chicken Nunukan.

Evaluasi Penerapan Standar Sanitasi dan Higien di Rumah Potong Hewan Kategori II (EVALUATION OF SANITATION AND HYGIENE STANDARD IMPLEMENTATION AT CATEGORY II ABATTOIR) Zikri Maulina Gaznur; Henny Nuraini; Rudy Priyanto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Meat is one of livestock industry products from abattoir. The existence of abattoir is necessary to ensure the meat product produced is safe, healthy and halal. This study was conducted to evaluate sanitation and hygiene standard implementation in category II abattoir. Total plate count (TPC), Salmonella sp, Coliform, and Escherichia coli were analized by using Bacteriological Analytical Manual (BAM) method. Analisis of water quality and liquid waste was done according to American Public Health Association (APHA) method. Based on laboratory test on TPC, Salmonella sp, Coliform, and Escherichia coli, the test results did not exceed the limit standard of SNI 3932:2008. The result of water quality and liquid waste analysis was around the threshold set by Indonesian Republic’s Regulation of Health Ministry No. 492/Menkes/Per/IV/2010 on the Quality Requirements of Water and Environment Regulation Ministry No. 5/2014 regarding Standard Liquid Waste. ABSTRAK Daging adalah salah satu produk industri peternakan dari usaha pemotongan hewan. Permintaan masyarakat terhadap daging sapi memengaruhi intensitas pemotongan, sehingga keberadaan rumah pemotongan hewan (RPH) diharapkan dapat menjamin kualitas daging secara aman, sehat dan halal. Penelitian ini bertujuan untuk mengevaluasi penerapan standar sanitasi dan higien di RPH kategori II. Pengujian total plate count (TPC) daging, Colliform, Escherichia coli, dan Salmonella sp berdasarkan metode Bacteriological Analytical Manual (BAM). Pengujian air bersih dan limbah cair menggunakan metode American Public Health Association (APHA). Hasil analisis mikrobiologi menunjukkan bahwa TPC, Salmonella sp, Coliform dan E. coli berada di bawah persyaratan SNI 3932:2008. Hasil analisis air bersih dan limbah cair sudah memenuhi baku mutu Peraturan Menteri Kesehatan Republik Indonesia 492/Menkes/Per/IV/2010 tentang Persyaratan Kualitas Air Bersih dan Peraturan Menteri Lingkungan Hidup 5/2014 Tentang Baku Mutu Air Limbah.

Hyaluronan Expression on Vitrified Oocytes Before and After In Vitro Maturation (EKSPRESI HYALURONAN PADA OOSIT YANG DIVITRIFIKASI SEBELUM DAN SESUDAH MATURASI IN VITRO) Zakiyatul Faizah; R. Haryanto Aswin; Hamdani Lunardhi; Widjiati Widjiati
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Oocyte vitrification is a major challenge in assisted reproductive technology. Oocyte vitrification with cumulus cells provide benefits in the process of maturation and fertilization. Vitrification leads to rapid temperature changes, therefore the decreasing in temperature could damage the cells even when the morphology was normal. Vitrification of mature oocytes is common because of its low sensitiveness towards low temperatures than immature oocytes. The aim of the research was to compare the effect of vitrification before and after in vitro maturation to the expression of hyaluronan. Maturation was operated in medium TC 50 ?L in CO2 incubators for 24 hours. Vitrification started with washing oocyte in PBS basic medium supplemented with 20% serum for 1-2 minutes, then in equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose (K1); 2) PBS + 20% serum + 0.25 M sucrose (K2); and 3).PBS + 20% serum + 0.1 M sucrose (K3). Immunocytochemical stain was performed to evaluate the hyaluronan expression. Remmele scale index (Immunoreactive score, IRS) was used to read the result. There was no differences of hyaluronan expression in oocyte and cumulus group of K1, K2 and K3 at p< 0.05, statistically. We concluded that there was no difference of hyaluronan expression on oocyte and cumulus between vitrified oocyte of pre and post in vitro maturation which indicated that oocyte could be vitrified in the immature and mature state.

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I G. Made Krisna Erawan

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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