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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Penggunaan Antibodi Anti-idiotipe sebagai Vaksin terhadap Streptokokosis (THE USING OF ANTI-IDIOTYPE ANTIBODY AS VACCINE AGAINST STREPTOCOCCOSIS) I Nyoman Suartha; Iwan Harjono Utama; Bambang Pontjo Priyosoeryanto; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 2 No 4 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

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Peningkatan Efikasi Vaksinasi pada Ikan Nila (Oreochromis niloticus) dengan Penambahan Ekstrak Tanaman Terung Asam dan Lempuyang Esti Handayani Hardi; Komsanah Sukarti; Maulina Anggridini
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was aimed to evaluate the Solanum ferox/SF and bitter ginger (Zingiber zerumbet/ZZ) in increasing the effectiveness of the Pseudomonas sp. (EP-02) vaccine through immersion in tilapia (Oreochromis niloticus). The dose used was 600 mg.L-1 of SF; 200 mg.L-1 of ZZ and vaccine bacterial density used 108 CFU.mL-1, the ratio between vaccine and extract was 1: 1: 1. The test were begun with immersion the tilapia in the vaccine and extract for 20 minutes, the challenges test was done on day 7th (d7), 14th (d14) and 21th (d21) post-vaccination with the combined bacteria Aeromonas hydrophila (EA-01) and Pseudomonas fluorescens (EP-01) (concentration of each bacteria was 105 CFU.mL-1) through intramuscular injection of 0.1 mL each fish. Abnormal swimming patterns, pathology of anatomy, total mortality, survival rate, RPS, were measured at week 2 after the challenge test. Application of SF and ZZ extracts in vaccination can increase the non specific (haematology parameters) and specific immunity (phagocytic index and antibody) since d7 of vaccination. While the increasing fish immunity in vaccine groups occurred in d14 post vaccination. The extract administration in vaccination reduced the fish mortality; increased SR and RPS of tilapia faster than the vaccination group. In conclusion, addition of SF and ZZ extracts can increase the efficacy of vaccines faster and more effectively from day 7th of infection.
Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA) Trioso Purnawarman; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Agus Setiyono; Muharam Saepulloh
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.
Kualitas Spermatozoa Kauda Epididimis Sapi Bali dengan Penambahan Laktosa atau Maltosa yang Dipreservasi pada Suhu 3–5oC Jusak Labetubun; Isak Piter Siwa
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this research was to examine the effectivity of lactose or maltose in maintaining theviability of cauda epididymal spermatozoa at Bali cattle when preserved at 3-5 oC. Epididymal spermatozoawere collected by slicing, flashing and pressure of cauda epididymis with normal saline ( 0,9% NaCl). Thecollected spermatozoa were divided in equal volume into three different tubes where each tube was thendiluted with : i) 20 % egg yolk Tris solution (control), ii) 20 % egg yolk Tris solution + 0.6 gram lactose/ 100ml (L 0.6), iii) 20 % egg yolk Tris solution + 0.6 gram maltose/ 100 ml (M 0.6), respectively following this alltubes were kept in a refrigerator at 3-5 oC for six days. The quality of spermatozoa including percentage ofmotility, live, and intact plasma membrane (IPM) were evaluated daily for the six days period. Result atthe study showed that the mean spermatozoa concentration was 11.2 million cells/ml, whilst the mean atpercentage of motility, percentage of live, percentage of abnormality, percentage of cytoplasmic dropletsand percentage of IPM were 75.00%, 86.75%, 10.50%, 14.00% and 86.75%, respectively. At day 7 ofstorage, the percentage of motility, live, and IPM when the spermatozoa were preserved in media containinglactose was 39.00%, 51.40%, and 51.80%, respectively whilst when preserved in media containing maltosewas 38.00%, 49.80% and 52.00%, respectively. The percentage of motility, live and IPM was significantlyhigher (p<0.05) compared to the control. It is therefore concluded that the addition of lactose or maltose inthe preservation media could maintain the quality of the epididymal spermatozoa of Bali cattle.
Kualitas Semen Beku Kuda pada Pengencer Susu Skim dengan Konsentrasi Gliserol yang Berbeda Azizah -; Raden Iis Arifiantini
Jurnal Veteriner Vol 10 No 2 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cryoprotectant is one of determining factors for the success of stallion semen cryopreservation. Formost mammalian sperm cryopreservation,glycerol has been widely used as the cryoprotectant. Glycerol inhigh concentration is toxic to the sperm but in low concentration it has protective effect on the semen. Theobjectives of this experiment were to find appropiate glycerol concentration by using skim milk extender inorder to maintain motility and viability of spermatozoa of stallion frozen semen. Semen was collectedfrom three sexually mature stallions by using artificial vagina. The semen characteristics and qualitywere examined both macro and microscopically. Prior to extension, semen was centrifugated at 3000 rpmfor 15 minutes. The pellet was re-extended with skim milk extender with glycerol 5% (G5) 7.5% (G7.5), and10% (G10). The extended semen as then packed in mini straw (0.3ml), equilibrated at 5 oC for 2 hours,frozen in the liquid N2 vapor for15 minutes and then stored in liquid N2 container until futher evaluation.The frozen semen, was thawed at 37 oC for 30 seconds. The percentages of sperm motility and viabilitywere examihed. The result of this research showed that the sperm motility and viability in G5 was (23.8 %;35.6 %) significantly hihger (P<0.05) as compare a with G7.5 (18.1%; 28.6%) and G10. (11.8%; 23.1%). Therecovery Rate in G5 (35.4%) was significant high er (P<0.05) than G7.5 (26.9%) and G10 (17.6%). In conclusion,glicerol of 5% is recomended for cryopreservation of stallion semen extended with skim milk.
Tampilan Reproduksi Kambing Lokal Hasil Induksi Superovulasi dengan Ekstrak Pituitary Sapi (THE REPRODUCTIVE PERFORMANCE OF LOCAL DOES FOLLOWING INDUCED SUPEROVULATION WITH CATTLE PITUITARY EXTRACTS) Tongku Nizwan Siregar; Indah Kesuma Siregar; Teuku Armansyah; Syafruddin .; Arman Sayuti; Hamdani .
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was  to determine the effect of pituitary extracts on the reproductive threeperformance of local does. This study used 15 local female does that were divided into three treatmentgroups.  Each group consisted of five does.  All does were injected with 7.5 mg Prostaglandin F2? /PGF2?(Prostavet C, Virbac S.A) intramuscularlly, twice in 10 days interval. In group 1 (K1), the does wereinseminated 36 hours after last PGF2? administration.  In group II (KII), does were injected with 500 IUpregnant mare serum gonadotropine/PMSG (Folligon ™, Intervet, Boxmeer, Holland) on day 9 cycles andfollowed with injection of 7.5 mg PGF2? two days after. In group III (KIII), goats injected with pituitaryextract on days 9, 10, and 11 cycles with decreasing dose :  2, 1, and; 0.5 ml intramuscular injection locatedat some point, followed with injection of 7.5 mg PGF2? 2 days after. The does on the KII and KIII wereinseminated 36 hours after PGF2? administration. Parameters measured were oestrous percentage,pregnancy, kidding rate, twinning, total of litter size, and averages litter size.  Results showed that alldoes (100%) from all groups showed estrous and kidding rate. Percentage of pregnant does group I, II, andIII were  60, 40, and 80%, respectively. Percentage of twinning group I, II, and III were 0, 50, and 25%.  Thetotal of litter size group I and II were 3 does, and group III were 5 does. The averages litter size group I, II,and III were 1; 1.5; and 1.25 respectively. In conclusion, treatment with pituitary extracts can increasereproductive performance of local does.
Sonogram Dinamika Ovarium pada Kambing Kacang (Capra hircus) (SONOGRAM OF OVARIAN DYNAMIC IN KACANG GOAT (CAPRA HIRCUS)) Santoso .; Amrozi .; Bambang Purwantara; Herdis .
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A research was conducted to study the ovarian dynamics in kacang goats. Six non pregnant kacang goatswere used in this research. They were clinically healthy, 2 3 years old and showed normal estrous cycles. Theestrous cycles of the kacang goats were synchronized by using prostaglandin 0.5 mg/kg body weight duringluteal phase. The ovarian dynamics were observed daily by using transrectal ultrasonography. Ovarian dynamicswere characterized by follicular wave. The estrous cycle of kacang goats was 20.0±1.1 days. An ovulatoryfirst follicle wave was appeared on days 1-4, the second follicles wave was on days 3-7, the third folliclewave was on days 8-13, while the ovulatory follicular wave emerged on day 17-18. The dominant folliclesreached the maximum diameter in 3.1±0.6 days from wave emergence and the preovulatory dominantfollicle, the diameter was 6.5±0.5 mm. The corpus luteum of kacang goat was observed on 2.0±0.5 daysafter ovulation up to 2.8±0.8 days before the next ovulation. Follicular development consists of three orfour follicular waves and CL developments were static.
Respon Fagositosis Leukosit Polimorf Babi (in vitro) Terhadap Streptoccocus equi Subsp. Zooepidemicus (PHAGOCYTIC RESPONSE OF SWINE POLYMORPH LEUCOCYTES (IN VITRO) TO STREPTOCCOCUS EQUI SUBSP. ZOOEPIDEMICUS) Iwan Harjono Utama; Aisjah Girindra; Fachriyan Hasmi Pasaribu; I Wayan Teguh Wibawan; Endhie D. Setiawan; Gatut Ashadi; Aida Louise Tenden Rompis
Jurnal Veteriner Vol 1 No 1 (2000)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Respon Fagositosis Leukosit Polimorf Babi (in vitro) Terhadap Streptoccocus equi Subsp. Zooepidemicus (PHAGOCYTIC RESPONSE OF SWINE POLYMORPH LEUCOCYTES (IN VITRO) TO STREPTOCCOCUS EQUI SUBSP. ZOOEPIDEMICUS)
PATHOGENICITY STUDY OF ESCHERICHIA COLI ISOLATED FROM POULTRY ON BROILER CHICKEN AT 15-DAYS OF AGE Michael Haryadi Wibowo; Agnesia Endang Trihapsari Wahyuni
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study was conducted to determine the pathogenicity of Escherichia coli isolated from chickens. Three isolates, Kalasan (Ec/Kls/4/02), Sleman (Ec/Sl/1/02), and Wonosari (Ec/Wno/2/02) were firstly selected and inoculated into broiler chickens at 15 days of age. Two isolates, Kalasan (Ec/Kls/4/02) and Sleman (Ec/Sl/1/02) which showed clear clinical signs and macroscopic lesions, were cultured in Brain Hearth Infussion (BHI) media for 24 hours at 37oC. Serials ten fold dilution of the purified colonies, starting from 10 1 to 10 10 was prepared and 0,5 ml bacterial sample from each dilution were inoculated intraperitoneally into 5 chickens of 15 day-old. The inoculated chickens were monitored for seven days to observe the clinical signs and microscopic lesions. The infective dose (ID)50 of each isolate was determined by Reed and Muench method. The result showed that the two isolates were pathogenic to 15 day-old broiler chickens with specific lesions of pericarditis, perihepatitis, peritonitis, and airsacculitis. Their infective dose-50 was 2 X 10 2,6 bacterial cells per ml for Kalasan isolate and 2 X 10 1,8 bacterial cells per ml for Sleman isolate. It appeared that Kalasan isolate was more pathogenic than Sleman isolate.
Seroprevalensi dan Isolasi Toxoplasma gondii pada Ayam Kampung di Bali (SEROPREVALENCE AND ISOLATION OF TOXOPLASMA GONDII AMONG FREE-RANGE CHICKENS IN BALI) I Made Dwinata; Ida Bagus Made Oka; Nyoman Adi Suratma; I Made Damriyasa
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The prevalence of Toxoplasma gondii in free range chickens is a good indicator of the prevalence of T.gondii oocysts in the environment and the meat of chicken is considered one of the sources of the humaninfection. A study to determine the seroprevalence of T.gondii in free ranging chickens in eight regency inBali have been undertaken. More over, attempt to isolate T gondii was also performed from the copropositivesample. Seroprevalence was detected using modified agglutination test (MAT) and isolation of T.gondiiwere performed from organs (heart and brain) using pepsin-HCl digestion method. Further the pathogenicityof the isolate was determined by bioassay using mice. The result showed that the seroprevalence was24.8% (31 out of 125 chickens examined). T.gondii was found in 17 of the 31 seropositive chickens (55%)more over all isolates were a vitulent to the mice.

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