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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Penurunan Kadar Glukosa Darah dan Hitung Sel Kupffer Tikus Hiperglikemik Setelah Pemberian Dekok Daun Salam (INDONESIAN BAY LEAF DECOCTION COULD LOWER FASTING BLOOD GLUCOSE LEVEL ON HYPERGLYCEMIC RATS AND LOWER KUPFFER CELL COUNT) Yuliana .; Tangking Widarsa
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Diabetes mellitus (DM) is a metabolic syndrome due to pancreas failure to produce insulin, thereforehyperglycemia is the hallmark of DM. There is hyperglycemia in this disease. In the world, there isaround143 million diabetes mellitus patients. One of the deathcause in diabetes mellitus is chronic liver disease.Herbal medicine is thought to give less side effect than sintetic medicine. A traditional plant that hashypoglicemia property is decoction of Indonesian bay leaves. This study was aimed to study effectivity ofIndonesian bay leaf decoction in decreasing blood glucose level, pancreas and liver hemorrhage scores, andKupffer cell count on alloxan-induced Wistar rats. This Control Group Design used twenty four rats (threemonth old). The rats were divided randomly into four groups, i.e.: control, 1 mL of Indonesian bay leafdecoction (0.9 g/kg body weight (BW)), 2 mL of Indonesian bay leaf decoction (1.8 g/kg BW), and 3 mL ofIndonesian bay leaf decoction (2.7 g /kg BW). After one week adaptation the rats were inducedwithalloxan,then pretest for fasting blood glucose test was examined. Treatment was given for ten days and posttestblood glucose test was done afterwards. At the end of the experiment, all rats were sacrificed to collectpancreas, kidney, and liver. Those organs were stained with hematoxylin eosin. Levelof fasting bloodglucose, hemorrhage score, on pancreas and kidney and Kupffer cell count were analyzed by using Anova.In conclusion, Indonesian bay leaf decoction may lower fasting blood glucose level on hyperglycemic rats aswell as Kupffer cell count significantly, but it is not likely to give significant result in lowering of pancreasand kidney hemorrhage scores.
DEVELOPMENT OF MICE AND HAMSTER EMBRYOS IN KSOMAA AND HECM-6 MEDIUM Bayu Rosadi; M Agus Setiadi; Dondin Sajuthi; Arief Boediono
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of the present study was to investigate the viability of mice and hamster embryos developed in Kalium Simplex Optimized Medium amino acid (KSOMaa) and Hamster Embryo Culture Medium-6 (HECM-6) medium. Female DDY mice were superovulated by injection i.p. of 5 IU Pregnant Mare Serum Gonadotropine (PMSG) and 5 IU Human Chorionic Gonadotropine (hCG) in 48 h interval, hamster (Phodopus campbelli) injected by 2.5 IU PMSG and 2.5 IU hCG 48 h later. Then females were mated with fertile males. Eight-cell embryos were recovered at day 3 after natural mating. The mice embryos were cultured in KSOMaa+5% NBCS (New Born Calf Serum) (T1) and HECM-6+5% NBCS (T2), the hamster embryos were cultured in KSOMaa+5% NBCS (T3) and HECM-6 + 5% NBCS (T4) for further development at 37oC in a humidified atmosphere of 5% CO2 in air for 48 h. The examinations were replicated five times. The T1 embryos developed to compact morulla and early blastocyst 100% (140/140), 92.1% (129/140) to blastocyst and expanded blastocyst, and 22.9% (32/140) became hatching/hatched. The T3 reached 100% (60/60) to compact morulla and early blastocyst, 85.0% (51/60) blastocyst, and 48.3% (29/60) expanded blastocyst, no embryo observed hatching/hatced. The T2 embryos had more expanded blastocyst than T3 (P<0.05), hatching/hatched rate higher than T1 and T3 but lower than T4 (P<0.05). Shortly, KSOMaa enable to support 8-cell stage mice and hamster embryo, but the hamster embryo developed lower at expanded blastocyst stage. HECM-6 is more appropriate than KSOMaa to support 8-cell mice embryos development and suitable to develop 8-cell stage hamster embryos.
Profil Kimia Klinik Darah Sapi Bali (BLOOD CLINICAL CHEMISTRY PROFILES OF THE BALI CATTLE) Anak Agung Sagung Kendran; I Made Damriyasa; Nyoman Sadra Dharmawan; Ida Bagus Komang Ardana; Luh Dewi Anggreni
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

For the evaluation of clinical tests in veterinary laboratories a reference basis of normal values ofclinically healthy farm animals is essential. It is well known that variables such as breed, age and sex havean influence on many blood parameters. The reference value of blood chemistry of bali cattle is scanty. Theaim of the current study was to evaluate blood chemistry of bali cattle includes alanine aminotransferase(ALT), aspartate aminotransferase (AST), urea, creatinine and glucose, measured by an auto analyzer.These data were used to establish reference intervals in bali cattle of different ages and sex. Blood wascollected from 195 (21 young males, 54 adult males, 60 young females, and 60 adult females) clinicallyhealthy bali cattle by means of jugular venipuncture. Values from different age and sex were comparedstatistically. The results of the study was obtained that ALT and blood glucose significantly higher inmales Bali cattle (P <0.05) then in females. ALT, AST and urea were significantly higher in adults Balicattle (P<0.05) then in young Bali cattle. However, glucose of young Bali cattle was higher when comparedwith the adult. There was no significant difference in the creatinine levels between adults and the youngones. The results of the current study provide reference intervals for clinical chemistry of Bali cattle
Identifikasi Escherichia coli O157:H7 serta Deteksi Gen Shiga Like Toxin 1 dan 2 Asal Feses Hewan, Daging, dan Feses Manusia I Wayan Suardana; Wayan Tunas Artama; Widya Asmara; Budi Setiadi Daryono
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Escherichia coli O157:H7 with the ability to produce shiga-like toxin was isolated from beef, cattle,chicken, and human feces. Due to its importance to human health, it is necessary to identify the genesencoding the production of shiga-like toxin, stx1 and stx2 respectively to further understand the pathogenesis.Isolation of E. coli was done on Eosin Methylene Blue Agar (EMBA), followed by identification on SorbitolMacConkey Agar (SMAC), latex agglutination test, and H7 antiserum test, respectivelly. The existence ofgenes stx1 and stx2 in E. coli O157:H7 was confirmed molecularly using PCR method with specific primersLP 30/31 and LP 43/44, Stx2 (F)/Stx2 (R) respectively. Escherichia coli O157:H7 was isolated from 22 outof 344 samples (6,4%). Some isolates showed gene stx1 and stx2 was detected in two isolates as indicatedby a 384 bp band (stx1 gene), 584 bp and 1588 bp bands (stx2 gene) respectivelly. The results indicatedthat local isolates E. coli O157:H7 are potential as a zoonoses agent.
Lumba-Lumba Hidung Botol Laut Jawa Adalah Tursiops aduncus Berdasar Sekuen Gen NADH Dehidrogenase Subunit 6 (VERIFICATION BOTTLENOSE DOLPHINS FROM JAVA SEA IS TURSIOPS ADUNCUS BASED ON GENE SEQUENCES OF NADH DEHYDROGENASE SUBUNIT 6) Rini Widayanti; Yuda Heru Fibrianto; Woro Danur Wendo
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bottlenose dolphins (Tursiops sp.) is one of the aquatic mammals widely spread in the marines ofIndonesia archipelago, especially the Java Sea. The taxonomy of the genus Tursiops is still  controversial.The purpose of this study was to examine the molecular basis of Tursiops sp of Java sea marine origin onthe basis of its NADH dehydrogenase gene subunit 6 (ND6) sequences. Samples of blood were collectedfrom five male bottle nose dolphins from captivity of PT. Wersut Seguni Indonesia. DNA was isolated,amplified by polymerase chain reaction (PCR), sequenced, and analyzed the data using the MEGA v. 5.1program. The results of PCR amplification was 868 base pairs (bp), DNA sequencing showed that 528nucleotides were ND6 gene, nucleotide at the position of 387 could be used to distinguish the bottle nosedolphins Java marine origin with T. aduncus.   Filogram using Neighbor joining method based on thenucleotide sequence of the gene ND6, showed that bottle nose dolphins Java marine origin belong to groupof T. aduncus.
DAYA REDUKSI CENDAWAN DUDDINGTONIA FLAGRANS DAN SACCHAROMYCES CEREVISIAE TERHADAP LARVA CACING HAEMONCHUS CONTORTUS PADA DOMBA Riza Zainuddin Ahmad; Fadjar Satrija; Nampiah Sukarno; Fachrian Hasmi Pasaribu
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Salah satu masalah pada ternak domba adalah cacingan (haemonchosis) yang disebabkan oleh nematoda Haemonchus contortus
Aktivitas Antiplasmodium Daun Sernai (Wedelia Biflora) Berdasarkan Evaluasi Fungsi Ginjal dan Hati pada Mencit yang Diinfeksi dengan Plasmodium berghei Isa .; Rinidar .; Sugito .
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aimed to determine the antiplasmodial activity of sernai leaves (Wedelia biflora) extractagainst Plasmodium berghei which were infected to mice experimentally using the 4-days suppressive testmethod. The extraction was performed by maceration in three different solvents: ethanol, n-hexane, andethyl acetate. Inoculation of 1x106 P. berghei was performed in 60 Swiss mice intraperitoneally. Theantiplasmodial activity of the ethanol, n-hexana, and ethyl-acetate leaves extract at 4 different doses(100, 80, 60 and 40 mg/kg BW/day, respectively) were tested against P. berghei in vivo for 4 consecutivedays. Animals were given the leaves extracted orally. The negative-control animals were given dimethylsulfoxide diluents (DMSO) and animals in the positive-control were treated with P.berghei + DMSO.Parasitemia status were observed from day 1-4 by thin blood smear from tail and stained with Giemsa.On day 5, levels of urea, creatinine, SGPT and SGOT were measured. The results showed that extract ofsernai leaves using ethanol, n-hexane, and ethyl acetate had the antiplasmodial capacity on P.berghei inmice with effective doses (ED50) of 5.379 mg/kg BW, 25.306 mg/kg BW, and 27.442 mg/kg BW, respectively.The sernai leaves extract had the capability to maintain the liver function but not the kidney function.
Pertumbuhan Duddingtonia flagrans pada Air Liur, Larutan Rumen, Blok Komin, dan Agar Semen Riza Zainuddin Ahmad; Beriajaya -
Jurnal Veteriner Vol 11 No 1 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Duddingtonia flagrans is selected nematophagous fungi as biological control to nematode worm. Theaim of this research was to show that the survival of in vitro tested to D. flagrans mold growth is effectedby artificial saliva, rumen fluid, enzymes and agar-cement and comin block applicator. The testing wasdone two steps : Firstly the conidia’s was multicated and the secondly the growth of D.flagrans tested insaliva, rumen fluid and comin block, agar cement applicator. The result showed that mold could surviveand grow in condition such as in gastrointestinal fluid of sheep and in comin block and in agar-cementapplicator (1 and 2%). It can be concluded that D. flagrans could grow in medium containing rumen fluid,saliva, enzymes and comin block and agar-cement applicator.
Membran Spermatozoa Hasil Seksing Gradien Albumin Berpengencer Andromed dan Cauda Epididymal Plasma-2 Ditambahkan Kuning Telur (MEMBRANE OF SPERM FOLLOWING GRADIENT ALBUMIN SEXING USING ANDROMED AND CEP-2 SUPPLEMENTED WITH EGG YOLK) Rita Fitria Purwoistri; Trinil Susilawati; Sri Rahayu
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to determine sperm membrane stability after sexing with gradientalbumin (egg white) using andromed and CEP-2 supplemented with 10% egg yolk. The observations ofsperm membrane included membrane integrity, uncapacitated, capacitated  and acrosome reacted. Albumingradient was made by mixing egg white with andromed or CEP-2 supplemented with 10% egg yolk resultingin a concentration of egg whites 10%, 30% and 50%. Membrane integrity was observed using HOST(Hypoosmotic Swelling Test), while uncapacitated, capacitated and acrosome reacted sperm  were observedusing CTC (Chlortetracycline) staining. The results showed that both andromed and CEP-2 supplementedwith 10% egg yolk could protect membrane integrity, uncapacitated, capacitated and acrosome reactedsperm. Andromed and CEP-2 supplement with 10% egg yolk could reduce the demage of membrane integrityand uncapacitated sperm, whereas capacitated and acrosome reacted sperm could be kept low.
Staphylococcus aureus Penghasil Pigmen Kuning yang Diisolasi dari Kejadian Bumblefoot pada Broiler Lebih Patogen Dibanding Penghasil Pigmen Putih (STAPHYLOCOCCUS AUREUS PRODUCING YELLOW PIGMENT ISOLATED FROM BUMBLEFOOT CASE IN BROILER CHICKENS IS MORE PAT Khusnan .; Wahyu Prihtiyantoro; Mitra Slipranata
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to evaluate the correlation between the pigments produced by Staphylococcus aureusisolateswith their adhesion capability on epithelial cells and phagocytes activity of macrophages in vitro.Ten S.aureus isolatesfrom bumble foot and arthritis casesin broiler were used in this study. The adhesion assaywas performed using epithelial cell derived from human buccal epithelial cells whereas phagocytic activitywas conducted using mouse peritoneal macrophage cells.The results showed that S. aureus isolates frombroiler produced both yellow and white pigments. Staphylococcus aureus producing white pigment adheredto human buccal epithelial cells with the density of about 24,25 bacteria/cell, which were higher than thoseof Staphylococcus aureus producing yellow pigmentabout 23,78 bacteria/cell. Staphylococcus aureusproducing white pigment was more phagocytosed by macrophages than S. aureusproducing yellow pigment,with an average phagocytic activiry of19,86 bacteria/cellas compared to15,96of bacteria/cell, respectively.

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