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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Aktivitas Imunomodulator Ekstrak Buah Mengkudu pada Mencit yang Diinfeksi Staphylococcus aureus (IMMUNOMODULATORS ACTIVITY OF NONI (MORINDA CITRIFOLIA L.) FRUIT EXTRACT IN MICE INFECTED WITH STAPHYLOCOCCUS AUREUS) Zumrotul Mufidah; Muhaimin Rifa’i; Sri Rahayu
Jurnal Veteriner Vol 14 No 4 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aim was to determine the immunomodulatory activity of noni (Morinda citrifolia L.) fruitextract in mice infected with Staphylococcus aureus. Mice were divided into two group :  non-infected  andinfected. Non Infected group was without S. aureus infection whereas the infected group was infected withS. aureus. Group contain control, dose 1 (25 mg/kg BW), dose 2 (100 mg/kg BW), and dose 3 (300 mg/kg BW).Oral treatment carried out for 20 days in every morning and each sample was injected with  S. aureus atday 21 with 109 cell/mL. Relative number of T cell (CD4+, CD4+CD25+),) and cytokine interferon-ã fromCD4+ T cell (CD4+IFN-ã+) subsets was measured using the BD FACSCaliburTM Flowcytometer. Data wereanalyzed by using Analysis of Varians (p<0,05) and SPSS 16 for windows. The result showed thatadministration of noni crude extract was significantly change the relative number of CD4+, CD4+IFN-ã+,and CD4+CD25+ T cells. Treatment of noni crude extract in non-infection group could increase  relativenumber of CD4+, CD4+IFN-ã+  and CD4+CD25+ T cells that might be caused by active compounds of noni asmitogen.  Giving of noni crude extract in infected group could reduce  the relative number of CD4+, CD4+CD25+and CD4+IFN-ã+ T cells due to it  active compounds as anti-inflamation. Noni fruit extract can be used aspreventive therapy on S. aureus infection  because it contains active compounds as an anti-inflammationeffect.
Multiplex Reverse Transcription-Polymerase Chain Reaction untuk Deteksi Cepat Virus Flu Burung H5N1 (MULTIPLEX REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION FOR RAPID DETECTION OF H5N1 AVIAN INFLUENZA VIRUS) Raden Wasito; Hastari Wuryastuty; Gesit Tjahyowati; Sri Handayani Irianingsih; Tisna Tyasasmaya
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian influenza virus subtype H5N1 (AIV H5N1) is highly pathogenic and fatal in poultry. The virusis still endemic with low virulence rate, although it may play a critical role in causing high morbidity andmortality rates in poultry in Indonesia. In general, diagnostic approach for AIV H5N1 is based onconventional serological and viral isolation methods that have the potential to produce consumings oftime and relatively expensive cost within the laboratory without compromising test utility. Thus, amolecular approach of multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developedand applied for the detection of matrix gene type A influenza viruses, AIV subtype subtype H5hemagglutinin gene with simultaneous detection of N1 nucleoprotein gene. Thirty sera specimens fromthe diseased commercial chickens that were specifically amplified positive-RT-PCR for AIV H5N1 wereselected for mRT-PCR. The mRT-PCR products were visualized by agarose gel electrophoresis and consistedof DNA fragments of AIV of 245 bp, 545 bp and 343 bp for M, H5 and N1 genes, respectively. Thus, themRT-PCR that can rapidly differentiate simultaneously between these genes is very important for thecontrol and even eradication of AIV transmission in poultry in Indonesia.
Case Report : Urolith Surgical Removal in a Green Iguana (Iguana iguana) (LAPORAN KASUS : BEDAH PENGELUARAN BATU KANTUNG KEMIH PADA SEEKOR IGUANA HIJAU (IGUANA IGUANA)) Puveanthan Nagappan Govendan; I Gusti Made Ananthawijaya; Anak Agung Gede Jayawarditha
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.611 KB) | DOI: 10.19087/jveteriner.2018.19.1.143

Abstract

Two years old male Green Iguana (Iguana iguana) weighing 1.3 kilograms presented with a swelling mass in the abdomen. Clinical signs, palpation and radiography images indicated urolith in the urinary bladder. The mass was radiopaque and had a width of 37 mm and a length of 41 mm. Clinical findings, examination and radio-imaging confirmed urolithiasis in the urinary bladder. Coeliotomy and cystotomy were performed to remove the urolith. Two days after surgery, the iguana regained its appetite and normal urate secretion was observed. Prognosis is good when surgery is performed on a healthy animal.
Protein Kasar Plasma Seminalis Sapi Menurunkan Kejadian Nekrosis Spermatozoa Kambing yang Disimpan pada Suhu Dingin (BULL SEMINAL PLASMACRUDE PROTEIN DECREASING THE PERCENTAGE OF NECROSISIN GOATSPERMATOZOA THAT KEPT IN COLD TEMPARATURE) Suherni Susilowati; Hardijanto .; Indah Norma Triana
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the benefits of bullseminal plasma crude proteinin goatspermatozoa diluent to improve of frozen semen quality.This study consists of three treatments: (i)treatmentcontrol (P0,goat semen + diluents ; (ii) treatment I(PI, diluent + goat semen + bull seminal plasmacrudeprotein (1:1);and (iii)treatment II (PII, diluent + goat semen+ bull seminal plasma crude protein (1:2). Alltreatments were kept in arefrigerator (5oC) andobserved for necrosis of goatspermatozoa, daily. The highestspermatozoa necrosis was observed in the diluter without the bull seminal plasma crude protein eitheratday1, 2, 3, 4orday 5. The necrosis was statistically significant different (p<0,05) between P0, PI and PII.withthe lowest percentagenecrosis at PI. In conclusion, the addition of bull seminal plasma crude proteinin skim milk diluentwill decrease theincidenceof necrosis ingoat spermatozoa which were kept in coldtemperature.
Resistensi Antibiotik Terhadap Bakteri Escherichia coli yang Diisolasi dari Peternakan Ayam Pedaging di Kabupaten Subang, Jawa Barat (ANTIBIOTIC RESISTANCE TO ESCHERICHIA. COLI ISOLATED FROM BROILER FARMS IN SUBANG DISTRICT, WEST JAVA PROVINCE) Aji Barbora Niasono; Hadri Latif; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.825 KB) | DOI: 10.19087/jveteriner.2019.20.2.187

Abstract

Antibiotic resistance in avian bacterial pathogens is a common problem in the Indonesia poultry industry. The present study aimed to provide information on the present status of antibiotic resistance in Escherichia coli (E. coli) from a broiler farm environment. The research method uses a cross sectional study with a stratified random sampling pattern. E. coli was collected from litter using boot swab method at the broiler house farms in Subang District. E. coli isolates (n = 74) were obtained from 74 samples of boot swabs. The sample was isolated and identified morphologically and biochemically using the IMVIC test to obtain E. coli isolates. The test was followed by a sensitivity test for nine antibiotics, namely tetracycline, sulfamethoxazole, trimethoprim, ampicillin, nalidixic acid, ciprofloxacin, enrofloxacin, gentamicin and chloramphenicol by using the agar dilution method. The interpretation of the results of this sensitivity test refers to the Clinical and Laboratory Standards Institute CLSI VET01S (2015) and CLSI M100 (2018). Measurement of the level of knowledge is done using a structured questionnaire that has been tested for validity and reliability. E. coli was isolated from 74 samples of boot swab. 93.2% of E. coli samples were found to be MDR with the highest prevalence in four types of antibiotics at 40.5%. The most common resistances observed were against tetracycline 97.3%, sulfamethoxazole 87.8%, trimethoprim 74.3%, ampicillin 68.9%, nalidixic acid 64.8%, ciprofloxacin 45.9%, enrofloxacin 40.5%, gentamicin 28.4% and chloramphenicol 10.8%. The findings of this study revealed the high level of antibiotic resistance in broiler environment is worrisome and have negative implications for human and animal health.
Penggunaan Asam Lemak Sawit dalam Ransum untuk Meningkatkan Produksi Ayam Pedaging (THE USE OF PALM-TREE FATTY ACID IN RATION FOR THE IMPROVEMENT OF THE PRODUCTIVITY OF BROILER CHICKENS) Gusti Ayu Mayani Kristina Dewi
Jurnal Veteriner Vol 4 No 2 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Abstak dapat dibaca pada Full Text Abstract can be read at Full Text
Kombinasi Calcitriol dan Ethynil Ethyl Estradiol Meningkatkan Ekskresi Kalsium Urin dan Risiko Urolitiasis pada Tikus Ovariektomi Hartiningsih Hartiningsih; Ayu Dewi Puspitasari; Nurdysa Diliana Putri; Nurul Arifah; Wari Pawestri; Devita Anggraeni
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.684 KB) | DOI: 10.19087/jveteriner.2017.18.2.239

Abstract

The high excretion of calcium (Ca) in the urine can trigger the formation of urolith. Estrogen and calcitriol decrease urinary Ca excretion. This study aims to examine the combination of calcitriol and ethinyl ethyl estradiol against Ca urinary excretion and urolithiasis risk of ovariectomized rats. Twentyfive female Wistar rats eight weeks old were divided into five groups: i) normal control (NK); ii) ovariectomized control (OVK); iii) ovariectomized + calcitriol (OVD); iv) ovariectomized + ethinyl ethyl estradiol (OVE); and v) ovariectomized + combination calcitriol and ethinyl ethyl estradiol (OVDE). Seven weeks post-ovariectomy, each rat was put in an individual metabolic cage for the study of Ca balance. At day 4 to 7 of the study, residual feed, urine, and feces were collected daily for Ca analysis. At day 8, the rats were euthanized, the left kidney were collected for histopathological examination. The results showed that combination of calcitriol and ethinyl ethyl estradiol in OVDE rats caused Ca intake and Ca intestinal absorption significantly higher, and urinary Ca excretion tended to be higher although not significantly different compared to OVK rats. Calcium excretion in OVK rat urine was higher compared to the NK rats. The kidney histopathological changes of OVK rats were not different from the NK rats. Histopathological examination of the OVDE group kidney showed protein deposition in the capsular of Bowman’s capsule and proximal tubules, atrophy of the proximal tubules, and necrosis, respectively. It is concluded that the combination of calcitriol with ethinyl ethyl estradiol in ovariectomized rats increased urinary Ca excretion and increased the risk of urolithiasis. ABSTRAK Tingginya ekskresi kalsium (Ca) dalam urin dapat menjadi pemicu terbentuknya urolit. Estrogen dan calcitriol menurunkan ekskresi Ca urin. Penelitian ini dilakukan bertujuan untuk mengkaji kombinasi calcitriol dan ethynil ethyl estradiol terhadap ekskresi Ca dalam urin dan risiko urolitiasis tikus ovariektomi. Sebanyak 25 tikus Wistar betina umur delapan minggu, dibagi menjadi lima kelompok (kontrol normal NK, kontrol ovariektomi OVK, ovariektomi + calcitriol OVD, ovariektomi + ethynil ethyl estradiol OVE, dan ovariektomi + kombinasi calcitriol dan ethynil ethyl estradiol OVDE). Tujuh minggu pascaovariektomi, setiap tikus dimasukan kandang metabolik individu untuk studi keseimbangan Ca. Pada hari ke-4 sampai 7 studi keseimbangan Ca, setiap hari sisa pakan, urin, dan feses dikumpulkan untuk analisis Ca. Pada hari ke-8 studi keseimbangan Ca, tikus dieutanasi, ginjal kiri diambil untuk pemeriksaan histopatologi. Hasil analisis menunjukkan bahwa kombinasi calcitriol dan ethynil ethyl estradiol pada tikus OVDE menyebabkan konsumsi Ca dan absorpsi Ca intestinal nyata lebih tinggi, dan ekskresi Ca dalam urin cenderung lebih tinggi meskipun tidak berbeda nyata dibanding tikus OVK. Ekskresi Ca dalam urin tikus OVK lebih tinggi dibanding tikus NK. Gambaran histopatologi ginjal OVK terlihat tidak berbeda dengan tikus NK. Histopatologi ginjal OVDE terlihat endapan protein dalam ruang kapsuler kapsula Bowman’s dan tubulus proksimalis, tubulus proksimalis atropi, dan nekrosis. Dari hasil penelitian disimpulkan bahwa kombinasi calcitriol dengan ethynil ethyl estradiol pada tikus ovariektomi meningkatkan ekskresi Ca urin dan meningkatkan risiko urolitiasis.
Peningkatan Kualitas Spermatozoa Epididimis Kerbau Belang yang Dikriopreservasi dengan Beberapa Konsentrasi Sukrosa (THE QUALITY ENHANCEMENT OF EPIDIDYMAL SPERMATOZOA OF SPOTTED BUFFALO CRYOPRESERVING WITH VARIOUS SUCROSE CONCENTRATIONS) Muhammad Rizal; Herdis -; Yulnawati -; Hera Maheshwari
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penelitian dilakukan untuk mengetahui kualitas spermatozoa epididimis kerbau belang selama proses pembekuan (kriopreservasi). Bahan pengencer yang digunakan adalah AndroMed® sebagai kontrol (AM) serta kombinasi AndroMed® dengan 0,2% (S0,2) dan 0,4% sukrosa (S0,4). Hasil penelitian menunjukkan bahwa persentase motilitas spermatozoa epididimis kerbau belang setelah thawing dalam bahan pengencer AM (41%), nyata (P<0,05) lebih rendah dibandingkan dengan S0,2 (46%) dan S0,4 (46%). Demikian pula halnya dengan persentase hidup spermatozoa setelah thawing dalam bahan pengencer AM, S0,2, dan S0,4 secara berturut-turut adalah 52,2; 59,8 dan 60,8% (P<0,05). Sementara itu, tidak terdapat perbedaan nyata (P>0,05) pada persentase membran plasma utuh (MPU) setelah thawing dalam ketiga bahan pengencer. Persentase MPU setelah thawing perlakuan AM, S0,2, dan S0,4 adalah masing-masing 68; 68,8 dan 66,8%. Dapat disimpulkan bahwa penambahan sukrosa sebagai krioprotektan eksternal ke dalam bahan pengencer AndroMed® dapat meningkatkan kualitas spermatozoa epididimis kerbau belang setelah thawing.
Pemakaian Herbal Serbuk Biji Pepaya Matang dalam Pengendalian Infeksi Ascaris suum pada Babi Ida Bagus Komang Ardana; I Made Bakta; I Made Damriyasa
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study on vermisidal effect of ripe papaya seed powder against egg and worm of A.suum in landracepig was done. Twenty four pigs age 8-12 weeks were used in this study using pre-test and post-test groupexperimental design. The pigs were randomly divided into four groups : a group of pigs with no treatmentas a control (P0) and other three groups were given 1 g/kg body weigh (P1), 3 g/kg bw (P2) during threeconsecutive days and treated with 12.5% zodalben oral 0.5 mg of albendazole (P3). The number of A.suumand its eggs per gram (EPG) were identified by using international harmonization and anthelminticefficacy guideline. Then follow by observation and calculation of EPG, fecal egg count reduction (FECR),and the efficacy of the powder for seven days. The result showed there was a significant lower number ofA.suum infestation in the treated group of pigs compared to the control group as it was shown by theincrease of the efficacy against worms and the FECR value. However, there were different effects on theefficacy and FECR between the group that treated with 1 g/kg bw of the ripe papaya seed powder (efficacy75% and FECR 92.4%) compared to the groups that treated with 3 g/kg bw of the seed powder, and treatedwith zodalben 12.5% (both efficacy and FECR were 100%). It can be concluded that herbal powder of ripepapaya seed has a potential effect to be vermisidal, especially for A.suum.
Deteksi Produksi Toksin Stx-1 dan Stx-2 dari Escherichia coli O157:H7 Isolat Lokal Hasil Isolasi Feses dan Daging Sapi I Wayan Suardana; I Gusti Made Krisna Erawan; Bambang Sumiarto; Denny Widaya Lukman
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Shiga toxin produced by Escherichia coli O157:H7 can cause outbreaks and sporadic cases of serioushuman diseases. The diseases are indicated by hemorrhagic colitis and hemolytic uremic syndrome. Meatand meat products have been identified as vehicles of food borne disease caused by E.coli O157:H7. Themain aim of this research was to identify the correlation between the level of E.coli O157:H7 contaminationand the presence of Shiga toxin (Stx1 and Stx2) by applying method of Vero toxin Escherichia coli-ReversePassive Agglutination Test (VTEC-RPLA). The results showed that 3 of 7 isolates and 1 of 4 isolatesisolated from feces of cattle and beef, respectively produced Stx 1 (VT1). In the detection of Stx 2 (VT2), 4of 7 isolates and 1 of 4 isolates, isolated from the same samples were found to produce this toxin.According to all isolates, in this research showed, 1 isolate was found to produce VT2, 4 isolates to produceboth VT1 and VT2, while 6 isolates showed negative results either to VT1 or VT2.

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