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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Lokasi Gigitan Secara Anatomi dan Waktu Kematian Pascagigitan Anjing Rabies pada Korban Manusia di Bali (THE ANATOMICAL LOCATIONS OF BITE AND THE TIME OF DEATH IN HUMAN VICTIMS BITTEN BY RABIES INFECTED DOGS IN BALI) I Ketut Suatha; Calvin Iffandi; Yunita Lestyorini; Sri Milfa; Abdul Azis Nasution; Nurul Faiziah; Rasdiyanah .; Imam Sobari; Herbert .; Ni Wayan Listyawati Palgunadi; I Made Kardena; Sri Kayati Widyastuti; I Wayan Batan
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rabies was a new emerging disease in Bali. After the first case of rabies in 2008 there were morethan one hundred of human victims in Bali. The aim of this study was to report the anatomical locationof human body bitten by rabies infected dogs in Bali, and also the day of victims death after the bitten. Aretrospective cross-sectional review of rabies incidences from September 2008 to the end of 2011 was usedin this study. A total of 122 rabies human victims data were used as a population sample. The data on theprofiles of individuals victim were colected , such as the age, gender, living place, anatomical bite site onhuman body, bite date, and type of biting animal. The obtained data were analyzed using descriptiveanalysis. The result showed that the anatomical location of human body that bitten by rabies infected dogwas occurred on leg (52%), hand (32%), body (6%), and head (4%). Of 122 victims, 61.5% were men and38.5% were woman. The death time of the human rabies victims in average occurred at day 95th after thebite date, and time of the victims death depended on the anatomical location of the bite. Bite that occurredon the head caused death in average on day 19th, on the body on day 83th, on the hand on day 122nd, and onleg on day 166th. In conclusion, the most victims of human rabies in Bali was men and the death was foundoccurred on day 95th after the bite date. The bite at the head caused death more quickly than the other partof human body.
Pakan Aditif Ekstrak Etanol Lumut Hati Meningkatkan Pertumbuhan Morfologi Duodenum dan Perkembangan Otot Dada Ayam Pedaging (SUPPLEMENTATION OF ETHANOLIC EXTRACT OF MARCHANTIA IN PRE-STARTER DIET IMPROVES THE GROWTH OF DUODENUM AND PECTORALIS THORACICUS Hendry Tri Sakti Surya Gunawan Saragih; Muhammad Faisal Alawi; Muhammad Rafieiy; Indra Lesmana; Heri Sujadmiko
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.81 KB) | DOI: 10.19087/jveteriner.2017.18.4.617

Abstract

This study aimed to determine the effectiveness of ethanolic extract of Marchantia as an alternative additive in broiler’s chicken feed. The research was designed by using 5 treatment groups with 20 day-old chick broilers in each group and the treatment was done until the sample aged 14 days. The treatment groups consisted of basal feed and ethanolic extract of Marchantia in 0; 62.5; 250; 500; 1000 mg/kg basal feed. Data were analyzed by using one way ANOVA test followed by Tukey test with significance P<0.05. The results showed that at 14 days old of the sample, the weight gain of the broilers in the treatment group 1, 2, 3, and 4 were higher than the control group; Feed Conversion Ratio of treatment group 4 was better than the control group and other treatments; the small intestine morphology of villus heights, crypt depth, and villus/crypt ratio showed that the treatment group 3 and 4 were better than the control group; the pectoralis muscle’s weight, muscle’s area, and myofiber’s area showed that the treatment group 1 and 3 were better than the control group. The study indicates that the ethanolic extract of Marchantia spp. with concentration of 750-1000 mg/kg basal feed has effective ability as an alternative additive in broiler’s chicken feed.
Diagnosis Infeksi Streptococcus suis serotipe-2 pada Babi Secara Serologi dengan Muramidase Released Protein (SEROLOGICALLY DIAGNOSE OF STREPTOCOCCUS SUIS SEROTYPE-2 INFECTION IN PIGS BASED ON MURAMIDASE RELEASED PROTEIN) Siti Isrina Oktavia Salasia; Clara Ajeng Artdita; Mitra Slipranata; Sidna Artanto
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Streptococcus suis is a bacterial pathogen causing disease of pigs that characterized by meningitis,bronchopneumonia, arthritis, pericarditis, polyserositis and septicaemia. S. suis especially serotype 2 caninfect human (zoonotic) with a special symptom of meningitis. The aim of this research was to detect S.suis infection based on muramidase released protein (MRP), as an important virulence marker of S. suis.S. suis serotype 2 strain P171 with phenotype of MRP+EF+ was used in this research. The MRP antigen wasextracted using lysozyme and separated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE). Balb/c mice were imunized with 136 kDa MRP to produce antibody against MRP. Theantibody was evaluated by using enzyme linkage immunosorbent assay (ELISA). The results of the researchshowed that the antibody against MRP with molecular weight of 136 kDa could be produced on Balb/Cmice with the highest absorbance of 3,889 and could be used to detect field sera from infected pigs with200x dilution using ELISA antigen capture. Antibody against MRP could detect serologically of S. suisinfection in pigs in Papua with 50% seropositivy by using ELISA antigen capture and 40% by using dot blot.
Terapi Ammonium Khlorida-Asam Askorbat untuk Menurunkan Tingkat Keasaman Urin dan Kristalisasi Struvit pada Kucing Urolithiasis (THERAPY AMMONIUM CHLORIDE-ASCORBIC ACID FOR LOWERING URINE ACIDITY AND STRUVITE CRYSTALLIZATION IN FELINE UROLITHIASIS) Alfarisa Nururrozi; Soedarmanto Indarjulianto; Yanuartono Yanuartono; Hary Purnamaningsih; Sitarina Widyarini; Slamet Raharjo; Dhasia Ramandani
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.516 KB) | DOI: 10.19087/jveteriner.2019.20.1.8

Abstract

Struvite/Magnesium ammonium phosphate (MAP) is common causes of feline urolithiasis. Prevent the formation of MAP crystallization can be treated by adjusting the pH urine in acid conditions. Urine with an acid pH will decrease struvite activity product (SAP) by preventing the phosphate deprotonation process which is the main constituent of struvite components. This study aims to determine the potential of a combination of ammonium chloride (NH4Cl) and ascorbic acid to decrease SAP by retain the urine pH under normal conditions. Twelve male cats, 2-5 years old and body weight 3.0 ± 0.8 kg were diagnosed struvite urolithiasis used for this study. Cats have a9clinical history of hematuria, dysuria, polyuria, and stranguria. Diagnosis of urolithiasis performed by clinical examination, USG, x-rays, and urinalysis. Cats that found struvite urolith, treated with 200 mg/kg NH4Cl and 100 mg/kg ascorbic acid orally twice a day. The research objects were observed included urine pH and crystaluria density. The data were analyzed using analisis of varian. The result of this study showed NH4Cl and ascorbic acid treatment can decrease the urine pH at 8 cats (89%) with an average pH 6.3 ± 0.3. Microscopic examination of the urine showed the struvite crystallization more infrequently than before therapy. The study concluded that the combination of NH4Cl and asam askorbat can lower the urine pH and reduce struvite crystal density in the urine.
Mengebiri Kuda Posisi Berdiri dengan Metode Terbuka (THE CASTRATION OF STANDING HORSES BY OPEN METHOD) Luh Putu Listriani Wistawan; Ni Nyoman Sutiati; I Gusti Agung Gede Putra Pemayun; I Wayan Batan
Jurnal Veteriner Vol 2 No 3 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Abstak dapat dibaca pada Full Text Abstract can be read at Full Text
Sekuen Nukleotida Gene Shiga like toxin-2 dari Isolat Lokal Escherichia coli O157:H7 asal Hewan dan Manusia (NUCLEOTIDES SQUENCES OF SHIGA-LIKE TOXIN 2 GENES OF ESCHERICHIA COLI O157:H7 LOCAL ISOLATES ORIGINATED FROM ANIMALS AND HUMAN) I Wayan Suardana; Dyah Ayu Widiasih; Komang Januartha Putra Pinatih
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.77 KB) | DOI: 10.19087/jveteriner.2017.18.1.83

Abstract

Animals/livestock, especially cattle, are known as the main reservoir of Escherichia coli O157: H7. As the only one of zoonotic E. coli, the pathogenicity of these bacteria is determined by its ability to produce one or more very potent cytotoxin known as Shiga-like toxin (Stx) or verocytotoxin, particularly of the Stx2 type that is closely related to the incidence of hemolytic uremic syndrome (HUS) in humans. This study analyzed the nucleotide sequences of stx2 gene between isolates from animals and humans in an effort to assess the potential zoonoses of the agent. The research activity was initiated by cultivating 20 isolates of E. coli O157:H7 collection based on result in the previous study i.e. 2 isolates originated from cattle feces, 2 isolates originated from beef, 2 isolates originated from chicken feces, 2 isolates originated from human feces, and 12 non-clinical isolates originated from human fecal who were suffering with renal failure. All isolates were confirmed on selective medium Sorbitol MacConkey Agar (SMAC) followed by testing on aglutination O157 latex test, and H7 antisera. Molecular analysis of stx2 gene covering open reading frame (ORF) of the stx2 gene was performed using the primer which was designed by researcher i.e. Stx2 (F)/Stx2 (R). The results showed, there were 2 isolates i.e. KL-48 (2) originated from human feces and SM-25 (1) originated from cattle feces were positive for carrying a stx2 gene, which was marked by the 1587 bp PCR product. Analysis of sequencing showed both isolates had identical to stx2 nucleotide squences with E. phaga 933 as well as E. coli ATCC 933. These results indicate the both local isolates are potential as zoonotic agents with clinical effects similar to E. phaga 933 and E. coli ATCC 43894. ABSTRAK Hewan ternak khususnya sapi, dikenal sebagai reservoir utama Escherichia coli O157:H7. Sebagai satu-satunya serotipe E. coli yang bersifat zoonosis, patogenitas bakteri ini ditentukan oleh kemampuannya untuk menghasilkan satu atau lebih cytotoxin yang sangat potensial yang dikenal dengan nama Shiga-like toxin (Stx) atau verocytotoxin, khususnya dari jenis Stx2 yang terkait erat dengan kejadian hemolytic uremic syndrome (HUS) pada manusia. Studi ini bertujuan menganalisis susunan nukleotida dari gen stx2 antara isolat asal hewan dan manusia dalam upaya mengkaji potensi zoonosis yang ditimbulkannya. Kegiatan penelitian diawali dengan kultivasi 20 isolat E. coli O157:H7 koleksi hasil penelitian sebelumnya dengan rincian dua isolat asal tinja sapi, dua isolat asal daging sapi, dua isolat asal tinja ayam, dua isolat asal tinja manusia non-klinis, dan 12 isolat asal tinja manusia klinis (asal penderita gagal ginjal). Isolat sebanyak 20 tersebut dikonfirmasi pada media selektif sorbitol MacConkey agar (SMAC) yang dilanjutkan dengan uji latex O157 aglutination test serta uji antiserum H7. Analisis molekuler komplit gen stx2 yang meliputi open reading frame (ORF) dari gen stx2 dilakukan menggunakan primer rancangan peneliti yaitu Stx2(F)/Stx2(R). Hasil penelitian menunjukkan bahwa ada dua isolat yaitu KL-48 (2) asal tinja manusia dan SM-25 (1) asal tinja sapi positif membawa gene stx2 yang ditandai dengan produk PCR 1587 bp. Analisis hasil sekuensing menunjukkan kedua isolat memiliki susunan gene stx2 yang identik dengan E. phaga 933 dan E. coli ATCC 43894. Hasil ini mengindikasikan kedua isolat lokal berpotensi sebagai agen zoonosis dengan efek klinis yang serupa dengan E. phaga 933 dan E. coli ATCC 43894.
Karakteristik Aliran Darah pada Katup Semilunar Aorta Anjing Kampung yang Dinilai dengan Pulsed Wave Doppler Ekhokardiografi (CHARACTERISTICS OF BLOOD FLOW IN SEMILUNAR AORTA VALVE OF MONGREL DOG ASSESSED BY PULSED WAVE DOPPLER ECHOCARDIOGRAPHY) Deni Noviana; Fitri Alham
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study was to determine the characteristics of blood flow which passed through theaortic valve of normal Indonesian mongrel dogs using trans-thoracic Pulsed Wave Doppler (PWD)echocardiography. Eight (8) adult dogs (three male dogs and 5 female dogs) with an average age 3.31 ± 0.59years and average body weight 12.45 ± 1.30 kg were used in the study. Trans-thoracic PWD echocardiographyexaminations were performed in conscious/unsedated animals at the left lateral recumbence position. Theinstruments used in this study were: i) two dimensional ultrasound device and ii) phased array transducersmall foot print with 3.7 – 5.0 MHz frequency. Six elements of PWD were measured: i) heart rate (HR); ii)peak velocity (Vpeak); iii) velocity time integral (VTI); iv) mean pressure gradient (MPG); v) pulsatilityindex (PI); and vi) ratio of systole-diastole (S/D). Results showed the typically characteristics of blood flowin semilunar aorta valve is demonstrated by the only one peak wave (peak velocity = Vpeak) upon examinationby placing the Doppler scan volume right on the aorta valve. In general, all six elements of PWD measuredwere found to be higher in female dogs compared to male dogs. However, these differences were notstatistically significant (P>0.05).
Karakteristik dan Patogenisitas Streptococcus Agalactiae Tipe ?-hemolitik dan Non-hemolitik pada Ikan Nila Esti Handayani Hardi; Sukenda -; Enang Harris; Angela Mariana Lusiastuti
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Streptococcus agalactiae was isolated from cultured Nile tilapia (Oreochromis niloticus) in Cirata gulfand Klaten. The isolates were Gram positive cocci, oxidative fermentative positive, motility, and catalasenegative, grown on media containing NaCl 6.5%, ?-haemolytic and non-haemolytic. Two types of S. agalactiae(?-haemolytic and non-haemolytic) are different from their variety of sugars fermentation. Strains ?-haemolytic can ferment more sugars, including arabinose, sorbitol, lactose, and trehalose. Experimentalinfectivity trials on Nile tilapia (size 15 g), non-haemolytic type showed more virulent. This type causedfaster mortality, more severe behavior changes, and pathology changes than â-haemolytic type. NonhemoliticS. agalactiae caused 48% mortality 6-24 hours after injection, whereas â-haemolitic type caused17% mortality which it occured in 48 hours after injection (mortality of fish control 2,22%). Behaviordisease signs caused by non-haemolitic S. agalactiae started to happen 6 hours after injection whereas 12hours in ?-haemolytic type infection. Histopatological changes were observed on fish eye, spleen, andbrain. Hyperaemia, hyperthrophi, degeneration, and necrosis were also found on infected fish. Thisresearch was concluded that non-haemolytic of S. agalactiae was more virulent than ?-haemolytic.
Kajian In vitro Aktivitas Sel-Sel Trofoblas Blastosis Mencit Aging dan Pengaruhnya terhadap Kegagalan Implantasi Ita Djuwita; Roza Helmita; Adi Winarto; Wahyudin -
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objectives of this in vitro study were to investigate the hatching rate, the outgrowth diameter andthe activity of mitochondria Nicotiamide Adenin Dinucleotide Dyhidrogenage (NADH)-CoQ reductase ofblastocysts trophoblast cells from aging mice. Blastocysts of aging (age >12 months) and young productive(age 2 months) mice were collected from the cornua utery at day-4 of pregnancy and were cultured inmDMEM medium supplemented with 10% New Born Calf Serum (NBCS), 10% ITS, and 50 ?g/mlgentamicine, in 5% CO2 incubator at 37°C for 10 days. The blastocysts hatching rate and the trophoblastsmonolayer were examined for their diameter outgrowth and the NADH-CoQ reductase activity. The resultsshowed that the hatching rate, the trophoblast outgrowth diameter and the activity of NADH-CoQ reductaseof blastocysts collected from productive mice were significantly higher than those collected from the agingmice (P<0,05). It can be concluded that the impairment of blastocysts implantation especially, in agingmice were caused by the low activity of the NADH-CoQ reductase that play important role in energyproduction needed for the hatching and trophoblast outgrowth.
Metode Direct Polymerase Chain Reaction untuk Melacak Campylobacter sp. pada Daging Ayam (DIRECT POLYMERASE CHAIN REACTION METHOD FOR DETECTION CAMPYLOBACTER SP. OF POULTRY MEAT) Andriani .; Mirnawati Sudarwanto; Surachmi Setiyaningsih; Harsi Dewantari Kusumaningrum
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Campylobacter sp. is the most commonly reported as agent of foodborne zoonosis causing acutegastroenteritis in humans. Poultry meat is considered as a major source of C. jejuni infection in human.The conventional methods for detecting foodborne bacteria is time-consuming which rely on the of thebacteria in culture media, followed by biochemical identification. In this study polymerase chain reaction(PCR) technique was used for rapid identification of the pathogenic Campylobacter sp. The samples usedwere 298 chicken carcass with sold in supermarkets and traditional markets, and were carried out inaccordance the isolation protocol ISO/ DIS 10272-1994. Identification was performed using biochemicalAPI Campy. The direct PCR (DPCR) assay with two sets of primers was employed for isolation andidentification of C. jejuni and C. coli. The result of the isolation and identification both by conventional orPCR methods showed that chicken carcasses both from supermarket and traditional market werecontaminated with C. jejuni and or C. coli. Prevalence of Campylobacter sp. contamination in chicken meatwas higher by DPCR (62.6%) than by conventional (19.8%), indicating that DPCR technique was moresensitive than conventional method with detection limit for C. jejuni was103 cfu/ml.

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