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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Teripang Pasir Meningkatkan Kandungan Antioksidan Superoksida Dismutase pada Pankreas Tikus Diabetes (SEA CUCUMBER INCREASED ANTIOXIDANT SUPEROXIDE DISMUTASE IN THE PANCREATIC TISSUE OF DIABETIC RATS) Tutik Wresdiyati; Ani Karmila; Made Astawan; Rahman Karnila
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

High level of blood glucose is an indicator for diabetes mellitus (DM) condition. The condition iscaused by low level of insulin secretion or impairement of insulin receptor. The number of DM patientincreases every year. The World Health Organization reported that the number of DM patient in Indonesiawas the 4th highest in the world, after following China, India, and the United States of America, respectively.This study was conducted to analyze the effect of sea cucumber (Holothuria scabra J) on the profile ofantioxidant copper, zinc-superoxide dismutase (Cu, Zn-SOD) in the pancreatic tissues of diabetic rats. Atotal of 25 male white rats (Sprague Dawley) were used in this study. They were divided into five groups;(1) negative control (KN), (2) positive control, diabetic rats (KP), (3) diabetic rats treated with hydrolyzatedprotein of sea cucumber (HDL), (4) diabetic rats treated with concentrated protein of sea cucumber (KST),and (5) diabetic rats treated with isolated protein of sea cucumber (ISL), respectively. Diabetic conditionwas obtained by alloxan injection 110 mg/kg bw. The treatments were done for 28 days. At the end oftreatment period, the rats were sacrificed and pancreatic tissues were collected and fixed in Bouin solution and then processed to paraffin embedding standard method. The tissues were then stained withimmunohistochemical staining techniques using monoclonal antibody of Cu, Zn-SOD. The results showedthat treatment of HDL, KST, and ISL of sea cucumber (Holothuria scabra J) increased the content ofantioxidant Cu, Zn-SOD either in Langerhans islets and acinar cells of pancreatic tissues-diabetic rats.The HDL of sea cucumber treatment gave the best effect in increasing the antioxidant content of Cu, Zn-SOD in pancreatic tissue of diabetic rats.
Ekstrak Air Umbi Ubijalar Ungu Menurunkan Total Kolesterol serta Meningkatkan Total Antioksidan Darah Kelinci I Made Jawi; Ketut Budiasa
Jurnal Veteriner Vol 12, No 2 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

High cholesterol diet can increase blood total cholesterol and the incidence of cardiovascular disease.Flavonoid from fruits and vegetables can prevent those conditions due to it’s antioxidant effect. The aimof this study was to examine hypolipidemic and antioxidant possible effects of water extract of purplesweet potato, on blood of local rabbit with high cholesterol diet. This studies used 20 female adult localrabbit which were divided into 2 groups by using randomized pre-test and post-test control group design.Before treatment blood samples were collected from auricula venous to examine the total cholesterol, LowDensity Lipoprotein(LDL), High Density Lipoprotein (HDL), triglyseride, malondialdehide (MDA), totalantioxidant, Serum Glutamic Oxaloacetic Transaminase (SGOT), Serum Glutamic Piruvic Transaminase(SGPT), and haemoglobin concentration, as pretest data. After pre-tests, group 1 was given high cholesteroldiet without water extract of purple sweet potato. In contrast group 2 was given high cholesterol diet withwater extract of purple sweet potato. After 3 months of the treatment the blood were collected for posttestexamination. The data was analysed by using t-test. The result showed a significant increase in totalcholesterol, LDL, SGOT,SGPT, and MDA in the blood of group 1( p<0,05), whereas there was a significantdecrease in HDL at the same group (p<0,05). However, after 3 months of the treatment, all of the parametersin group 2 (except the total cholesterol) were not significantly different with the pretest result (p>0.05),although there was a significant difference compared to the control group (p<0.05). Total cholesterol wassignificantly decreased after 3 months of treatment in group 2 (p<0,05). It can be concluded that waterextract of purple sweet potato can keep the lipid profiles in normal range values, prevent the increase ofMDA, increase blood total antioxidant, decrease blood total cholesterol, and safe for the rabbit liver thatgiven high cholesterol diet.
DETECTION OF NEWCASTLE DISEASE VIRUS BY NESTED REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION Anak Agung Ayu Mirah Adi; Nyoman Mantik Astawa; Ketut Santhia Adhy Putra; Yasunobu Matsumoto
Jurnal Veteriner Vol 9 No 3 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A study to utilize nested reverse trancriptase-polymerase chain reaction (RT-PCR) for the detection ofNewcastle disease virus (NDV) infection was carried out. NDV isolated from an outbreak in KarangasemDistrict of Bali, Indonesia was propagated in chicken embryos and its pathogenicity was determined byinoculation in 3 week-old chickens. Organ samples were collected from infected chickens for nested-RTPCR.Out of several different pairs of primers designed for study, 3 pairs of primers (F4s-F6r/F5s-F5r,F8s-F10r/F8s-F8r and F12s-F14s/F13s-F13r), each of which for first-round/nested PCR, was able to amplifyspecific regions of NDV genome. In the fist-round PCR, the PCR product of 1500 bps was not clearly visiblein the agarose gel following electrophoresis. In nested PCR a PCR product of 500 bp was clearly visible onagarose gel following electrophoresis. The 3 pairs of primers appeared to be potential for an accurate andrapid detection NDV in the infected host.
The Efficacy and Safety of Topical Gel Formulation of n-Hexane Fraction of Curcuma longa in Wound Healing of Hyperglycemic Mice Ietje Wientarsih; Wiwin Winarsih; Lina Noviyanti Sutardi
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Curcuma longa (turmeric) is one of the tropical plant belong to Zingiberaceae family. It has beneficialeffects in healing of variety diseases. The aim of this study was to evaluate the efficacy and safety of  atopical gel formulation of n-hexane fraction of Curcuma longa in the wound healing of hyperglycemic mice.A total of 30 hyperglycemic mice were divided into three groups. They were given topical application, NC asa negative control (gel base), PC as a positive control (Neomycine sulfate 5%), treated groups (HF = n-hexane gel). Histopathological examination of the skin was performed to evaluate the wound healing.  Theresult showed that, there were no changes on tha appearance of the gel preparation, physically.  Evethoughupon the pH determination of the gel at six month of storage at 40ºC there were significant difference.  Theeffect of n-hexane gel, in terms of decreasing the surface area of wound and histopathological characteristicswere significantly (p <0.05) different between the PC group and HF group.  Skin irritant test showedneither rashes, swelling, inflammation, redness, irritation, itching nor any other toxicity  symptoms followingapplication of the gel. Significant effect of n hexane fraction of C. longa gel on wounds without any adversedermal toxic effects sounds very promising and it may be employed in the management of diabetic wound.
Konsentrasi Serum Anjing yang Optimum untuk Menumbuhkan dan Memelihara Babesia canis dalam Biakan Tutuk Astyawati; Retno Wulansari; Cahyono -; Ferry Ardhiansyah; Ari Rumekso; Dhetty -
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of cultivation system in vitro is very important in the future study of Babesia canis. Theaim of this study was to cultivate B. canis in vitro using RPMI media with different concentration of dogsera. B. canis infected erythrocytes were collected from splenectomized infected dog. Parasites werecultivated with RPMI 1640 medium supplemented with normal dog sera at the concentration of 10%, 20%and 40%, the culture were then incubated in 5% CO2 , 37oC temperature for 17 days and subcultured every48 hours. The Percentage of Parasitized Erythrocytes (PPE) in culture with 10% dog serum was significantlylower than those 20%, and 40% The used of 20% and 40 % sera were better than 10%. It is recommendedthat 40 % serum can be used for initiation phase of cultivation, whereas 20% concentration were used formaintenance of the culture.
Immunological Detection of Newcastle Disease Viral Antigen in the Naturally Infected Chickens by Monoclonal Antibodies against Fusion-2 Protein Nyoman Mantik Astawa; Anak Agung Ayu Mirah Adi
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Monoclonal antibodies (mAbs) against Fusion (F)2 protein of Newcastle disease virus (NDV) wereproduced for the detection of the viral antigen in infected chickens. Cells derived from spleen of Balb/c miceimmunized with the virus were fused with mouse myeloma cells to generate hybridomas capable ofproducing mAbs against the virus. The hybridomas were screened by enzyme-linked immunosorbent assay(ELISA) for anti-NDV specific mAbs using crude viral antigen (allantoic fluid of NDV-infected fertile eggs)and normal uninfected allantoic fluid of fertile eggs as negative control. The NDV proteins reactive withmAbs were then determined by Western Blotting using purified NDV as antigen. The mAbs reactive withF2 (12.5 KDa) protein of NDV were then used for the detection of NDV antigen in both the allantoic fluidof NDV- infected chicken embryos and in organs of naturally infected chickens. The results showed that 2out of 5 mAbs produced were against F2 protein of NDV. By indirect ELISA, the mAbs were able to detectthe viral antigen in allantoic fluid of NDV infected fertile chicken eggs at the titre as low as 2-2 to 2-4 HAunits per 0.1 mL. NDV–antigen was also detected by immunoperoxidase staining in paraffin-embeddedtissues of NDV-infected chickens but not in normal uninfected chickens. The most prominent infection wasdetected in the gastrointestinal tract and the lung. The NDV antigen was also detected in other organssuch as the brain, spleen, and several other tissues. It is evident that mAbs produced against F2 proteinof NDV were applicable for use in the detection of NDV antigen in infected chickens.
Perkiraan Pasokan Nitrogen Mikrob pada Domba Ekor Tipis yang Diberi Bungkil Kedelai Terproteksi Tanin (ESTIMATION OF MICROBIAL NITROGEN SUPPLY IN THIN-TAILED SHEEP FED WITH TANNIN PROTECTED SOYBEAN MEAL) Husnaeni .; Sunarso .; Limbang Kustiawan Nuswantara
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This research was aimed to study the effect of soybean meal protection mangrove leaf tannin onpurine derivative (PD) excretion, estimated microbial nitrogen (MN) supply, and estimated efficiency MNsuplay based on DOMR in thin-tailed sheep. A total of 16 thin-tailed sheep aged eight months withaverage body weight of 11.81±1.65 kg, and were randomly fed with complete feed with tannin-protectedsoybean meal in different levels. The research was conducted according to completely randomized designwith four treatments and four replications. T0 (soybean meal without tannin), T1 (protected soybean meal0.5% tannin), T2 (protected soybean meal 1% tannin), and T3 (protected soybean meal 1.5% tannin).Research finding indicated that organic matter consumption (OMC), organic matter digestibility (OMD),and digestible organic matter in rumen (DOMR) were not affected (P> 0.05) by the treatment, however thetreatment had significant effects (P<0.05) against PD, estimated MN supply, and estimatedMN supply based on DOMR. Purine derivative, estimated MN supply, and estimated MN supply basedDOMR markedly decreased in the treatment of soybean meal protection 1.5% tannins. It was concludedthat soybean meal protection with mangrove leaf tannins was not significant against OMC, OMD andDOMR thin-tailed sheep, but protected soybean meal with tannins 1.5% affected on decreasing purinederivative excretion, estimated MN supply and estimated MN supply based on DOMR.
Performans Produksi dan Profil Metabolik Darah Domba Garut dan Jonggol yang Diberi Limbah Tauge dan Omega-3 (PRODUCTION PERFORMANCE AND BLOOD METABOLIC PROFILES OF GARUT AND JONGGOL RAMS THAT WAS FED MUNG BEAN SPROUT WASTE AND OMEGA-3) Gagah Hendra Wijaya; Mohamad Yamin; Henny Nuraini; Anita Esfandiari
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research objectives were to evaluate and compare of production performances and blood metabolicprofiles of garut and jonggol rams fed with mung bean sprout waste and omega-3. This research used 24rams aged of 1-2 years, consisted of 12 garut rams weighed of 36,43±1,45 kg (CV=13,87%) and 12 jonggolrams weighed of 23,09±0,57kg (CV=8,64%). Research used Completely Randomized factorial Design(CRD 2x3) with 2 main treatments x 3 factorials and four replications. The first factors as the maintreatments were different breeds of garut and jonggol. The second factors as the factorial treatments werethree different feed percentages which consisted of P0 (40% grass+60% concentrate I), P1 (40% mung beansprouts waste+60% concentrate II), and P2 (40% mung bean sprouts waste+60% concentrate II+omega-3).Data were analyzed by analysis of variance (ANOVA) and proceeded by Duncan Range Test. The resultsshowed that P1 had the highest performance on feed efficiency and average daily gain (ADG) parameters.Blood metabolic test showed that blood total protein, cholesterol, and glucose of all rams were normal. Inconclusion feeding rams with mung bean sprout waste as grass substitution have a positive effect on theperformance of the rams production performance.
Pemanfaatan Ekstrak Hipofisis Sapi untuk Memperbaiki Performans Reproduksi Induk Babi Post Partum (UTILIZATION OF CATTLE HYPOPHYSIS EXTRACT TO IMPROVE REPRODUCTIVE OF POST PARTUM SOWS) Tree May Thiessen Outang; Wilmientje Marlene Nalley; Thomas Mata Hine
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.637 KB) | DOI: 10.19087/jveteriner.2017.18.3.383

Abstract

This study aimed to evaluate study the influence of cattle hypophysis extract (HE) on the reproductive performance of post partum sows. Twenty landrace sows, 45 days after partum were divided into four groups, five sows each. All were injected with HE intramuscularly. Group I (P0) was injected with 3 ml of saline solution, Group II (P1) was injected with 3 ml contain of 10 mg/EH/Kg body weight (BW), Group III (P2) was injected with 3 ml contain of 20 mg/EH/Kg BW, Group IV (P3) was injected with 3 ml contain of 40 mg/EH/Kg BW. Every sows that showed the estrus symptom were inseminated artificially on the second days, twice each in the morning and evening. The intensity of estrus, length of estrus, number of service preconception (S/C), conception rate (CR), litter size, weight of piglet and milk production were recorded. The result showed that the score of estrus intensity in Group I, II, III and IV were 1.40; 2.40; 2.80 and 2.80 respectively with the length of estrus in each group were 4.40; 5.00; 5.00; 5.60 days respectively. The number of service in Group I, II, III and IV of each pregnancy were 4.40; 1.40; 1.00 and 1.20 with the CR were 0; 60; 100; and 80% respectively. The litter size produced of Group I, II, III and IV were 4.00; 6.00; 10.60; 8.40 respectively. The average of piglet body weight of Group I, II, III and IV were 1.20; 1.36; 1.56 and 1.35 kg respectively. The milk production of each sows of Group I, II, III and IV were 13; 41; 93; 88 g respectively. It can be concluded that cattle hypophysis extract improves the performance of post partum sows with the best level was 20 mg EH/kg of body weight. ABSTRAK Penelitian ini bertujuan mempelajari pengaruh pemberian ekstrak hipofisis terhadap performans reproduksi induk babi betina post partum. Penelitian ini menggunakan 20 ekor induk babi betina jenis landrace, dibagi kedalam empat kelompok perlakuan masing-masing terdiri atas lima ekor induk babi. Babi betina yang baru 45 hari menyapih anaknya diinjeksi dengan ekstrak hipofisis (EH) secara intramuskuler. Kelompok I (P0) diinjeksi dengan 3 mL NaCl fisiologis; Kelompok II (P1) diinjeksi dengan 3 mL NaCL mengandung 10 mg EH/kg bobot badan (BB); Kelompok III (P2) diinjeksi dengan 3 mL NaCl mengandung 20 mg EH/kg BB: Kelompok IV (P3) diinjeksi dengan 3 mL NaCl mengandung 20 mg EH/kg BB. Setiap induk babi yang menunjukkan gejala estrus diinseminasi pada hari kedua dengan interval dua kali, pagi dan sore. Peubah yang diamati adalah intensitas estrus, lama estrus, jumlah perkawinan per kebuntingan (S/C), angka konsepsi (conception rate/CR), litter size, bobot lahir anak, dan produksi susu. Hasil penelitian menunjukkan skor intensitas estrus kelompok I, II, III, dan IV masing-masing 1,40; 2,40; 2,80; 2,80 dengan lama estrus masing-masing kelompok secara berturut-turut 4,40; 5,00; 5,00; 5,60 hari. Nilai S/C masing-masing adalah 4,40; 1,40; 1,00; 1,20 dengan CR masing-masing 0; 60; 100; dan 80%. Total jumlah anak yang dihasilkan kelompok I, II, III, dan IV masing-masing adalah 4,00; 6,00; 10,60; dan 8,40 ekor. Rataan bobot lahir anak per ekor pada kelompok I; II; III; dan IV masing-masing adalah 1,20; 1,36; 1,56; dan 1,35 kg. Produksi susu per induk pada kelompok I, II, III, dan IV masingmasing adalah 13; 41; 93, dan 88 g. Simpulan dari penelitian ini adalah ekstrak hipofisis dapat meningkatkan performans induk babi post partum. Dosis terbaik adalah 20 mg EH/kg BB.
Fertilitas Semen Kerbau Rawa (Bubalus bubalis carabanensis) yang Diencerkan dengan Pengencer Nira Aren (FERTILITY OF SWAMP BUFFALO SEMEN (BUBALUS BUBALIS CARABANENSIS) DILUTED WITH SUGAR PALM JUICE EXTENDER) Muhammad Rizal; Muhammad Riyadhi
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Sugar palm juice (Arenga pinnata Merr.) can be used as semen extender because its various nutrientscontent which were needed for spermatozoa preservation. The objective of this research was to examineeffectivity of sugar palm juice as swamp buffalo semen extender in artificial insemination (AI) program.Semen of swamp buffalo were collected by using an artificial vagina. Fresh semen were evaluated anddivided in equal volume into four tubes and diluted with lactose extender containing 20% egg yolk (lactoseor control), 85% sugar palm juice + 15% egg yolk (PJEY15), 80% sugar palm juice + 20% egg yolk (PJEY20),and 75% sugar palm juice + 25% egg yolk (PJEY25), respectively. Diluted-semen were preserved inrefrigerator at 5oC, and quality of spermatozoa including percentages of motile spermatozoa, livespermatozoa, and intact plasma membrane (IPM) were evaluated every day for four days. A total of 13female swamp buffaloes were estrous synchronize with PGF2a, and six buffaloes were inseminated withlactose and seven buffaloes were inseminated with PJEY20. Results of this research showed that at dayfourpreservation, mean percentages of motile and live spermatozoa for lactose (36.67 and 51.83%) andPJEY20 (35.83 and 51.5%) were significantly (P<0.05) higher than PJEY15 (31.67 and 44%) and PJEY25 (30 and 45.33%), but no difference significant between treatment for IPM parameter. Pregnancy rate forlactose was 66.67% and 71.43% for PJEY20. Percentage of birth for lactose and PJEY20 were 66.67% and71.43%, respectively. In conclusion, sugar palm juice could be used as an alternative extender in semenpreservation process and AI of swamp buffalo.

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