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Contact Name
Made Ria Defiani
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jbiologi@unud.ac.id
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Managed by Biology Study Program, Faculty of Math and Natural Science, University of Udayana Jl. Raya Kampus Bukit Jimbaran, Kuta Selatan, Jimbaran, Badung, Bali 80361
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INDONESIA
Jurnal Biologi Udayana
Published by Universitas Udayana
ISSN : 14105292     EISSN : 25992856     DOI : https://doi.org/10.24843/jbiounud
Jurnal Biologi Udayana (p-ISSN 1410-5292 | e-ISSN 2599-2856 | DOI 10.24843/jbiounud) managed by the Department of Biology, Udayana University, published in two formats namely print and online regularly twice a year (June and December).
Articles 262 Documents
IDENTIFIKASI MOLEKULER BAKTERI STREPTOCOCCUS YANG BERASOSIASI DENGAN IKAN KERAPU YANG DIPERJUALBELIKAN DI PASAR-PASAR IKAN DI BALI I.B. Oka Suyasa; I.G.N.K. Mahardika; Yan Ramona
Jurnal Biologi Udayana Vol 18 No 1 (2014): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengidentifikasi bakteri Streptococcus sp. yang berasosiasi pada ikan Kerapu dengan menggunakan teknik-teknik molekuler Polymerase Chain Reaction (PCR) agar diperoleh identitas definitive bakteri tersebut. Dalam penelitian ini, sekuen 16S RNA marker dari bakteri-bakteri tersebut dijadikan target untuk diamplifikasi dengan metode PCR. Sekuensing produk PCR dilakukan di Berkeley Sequencing Facility, USA. Hasil sekuen nukleotida yang diperoleh selanjutnya di aligned dan di BLAST dengan menggunakan software MEGA 5.0. Hasil penelitian menunjukkan bahwa teridentifikasi sebanyak dua spesies bakteri Streptococcus (sementara diberi nama Streptococcus sp. isolat A dan B). Streptococcus sp. isolat A ditemukan pada semua lokasi pengambilan sampel ikan, sementara itu isolat B hanya ditemukan pada ikan yang diambil dari pasar ikan Karangasem. Berdasarkan nilai Haplotype Diversity (HD) nya, variasi genetik bakteri Streptococcus sp. pada ikan Kerapu tergolong sangat rendah.
Toxicity of extract seeds custard apple (Annona squamosa) on mortality of mosquito larvae Anopheles sp. Ni Putu Ristiati; Ni Putu Sri Ratna Dewi; Sanusi Mulyadiharja; Ni Wayan Gunia Prastuti
Jurnal Biologi Udayana Vol 23 No 1 (2019): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.59 KB) | DOI: 10.24843/JBIOUNUD.2019.v23.i01.p01

Abstract

The aimed of this study were to determine differences of toxicity of custard apple seeds extract at different concentrations on mortality the mosquito larvae of Anopheles sp. and to find the most effective concentration of extract towards the mosquitoes’ mortality. The experiment used Randomised Complete Design. The samples used were instar larvae of Anopheles sp. The crude extracts were obtained through maceration process. The research was conducted for 48 hours at Laboratory of Biology Department of Undiksha. Data were analysed using one way anava. The results showed that there were significant differences in which the value of f count > f table (16.500>2.87). It showed different concentration of toxicity from custard apple seeds extract on mortality mosquito larvae of Anopheles sp. The concentration of the extract was linier to the mortality of the Anopheles sp. The most effective concentration extract as a larvacide was 50 ppm.
PENGARUH PERLAKUAN DELIGNIFIKASI DENGAN LARUTAN NAOH DAN KONSENTRASI SUBSTRAT JERAMI PADI TERHADAP PRODUKSI ENZIM SELULASE DARI ASPERGILLUS NIGER NRRL A-II, 264 IDA BAGUS WAYAN GUNAM; KETUT BUDA; I MADE YOGA SEMARA GUNA
Jurnal Biologi Udayana Vol 14 No 2 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

his research was done in order to utilize agricultural waste (rice straw) as substrate to produce crude cellulase enzyme from Aspergillus niger. This research was conducted in two stages; the first stage was determination of the initial pH and fermentation time by pH treatment (5, 6 and 7) and fermentation time (7, 9 and 11 days). The second stage was determination of concentration of NaOH treatment and concentration of substrate, namely: concentrations of NaOH (2, 4 and 6%) and concentrations of substrate (1, 2 and 3% (w/v)). The results showed that the fermentation time of 9 days with the initial pH 6.0 was the optimal condition for production of crude cellulase enzyme from A. niger with rice straw as a substrate. The highest enzyme activity derived from interaction of delignification treatment with NaOH concentration of 6% and 2% rice straw substrate which produces endoglukanase enzyme activity (0.037 units/ml), filter paperase activity (0.033 units/ml), soluble protein (0.362 mg/ml), and specific activity of filter paperase (0.123 units/mg).
COMBINATION OF USING COW FECES POWDER AND PROBIOTIC IN FEED FOR LAYER NATIVE CHICKEN Suprio Guntoro; Anak Agung Ngurah Badung Sarmuda Dinata; I Wayan Sudarma
Jurnal Biologi Udayana Vol 20 No 2 (2016): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The development of free-range chicken farm is constrained by expensive food prices. On the other hand,feses of cattle had potential use as a source of feed. This study was conducted to determine the response ofcow feses and probiotics administration in the diet of laying range chicken. The study was conducted over 6months using 240 free-range chickens aged 8 months. Research was arranged in a completely randomized designwith 8 treatments and 5 replications. The treatment given were ration in accordance with the farmers formulacomprising: concentrate: 25%, corn 40% and rice bran 35% (P0) and ration as P0, with the substitution of ricebran with fermented cow feses each treatment with level 10 % (P1), 15% (P2) and 20% (P3) and ration as atP0, P1, P2 and P3 that received additional probiotic (Bio L) 1 cc / L of drinking water (P4, P5, P6 and P7). Theparameters were observed are: egg production (hen day), egg weight, feed intake, FCR (Feed Conversion Ratio)mortality and physical composition of eggs. The results showed that the diet which containing fermented cowfeses 20% did not decreased egg production and feed efficiency. The combination of fermented cow feses at thelevel of 20% with a probiotic (Bio L) 1 cc / L of drinking water showed that egg production  increased althoughit is not significant and reduce the number of FCR (Feed Conversion Ratio). In conclusion the use of fermentedcow feses up to the level of 20% in the diet both with and without the administration of probiotics, has no effecton physical composition of eggs.
CA2+ INTRASELULER TERLIBAT DALAM MEKANISME PEMBUKAAN STOMATA AKIBAT PENGARUH AUXIN MADE PHARMAWATI; MADE RIA DEFIANI; Ni LUH ARPIWI
Jurnal Biologi Udayana Vol 12 No 1 (2008): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Research was done to elucidate the role of Ca2+ intracellular in stomatal movement induced by auxin. Two types of auxin (IAA and NAA) increased stomatal opening and the increase was concentration dependent. The addition of EGTA, Ruthenium red and Procaine as the modulator of Ca2+ concentration , inhibited stomatal opening induced by auxin. EGTA and procaine significantly inhibited somatal opening at 100 uM and 1 mM. Ruthenium red worked at lower concentrations which were 10 uM, 50 uM and 100 uM on LAA-induced stomatal opening, while on NAA-induced stomatal opening, Ruthenium red had an effect at 10 uM, 50 uM, 100 uM dan 1 mM. It is suggested that Ca2+ involves in stomatal opening induced by LAA and NAA as signaling agent.
KETAHANAN Lactobacillus sp. ISOLAT SUSU KUDA SUMBAWA TERHADAP pH RENDAH DAN ASAM DEOKSIKOLAT SERTA KEMAMPUANNYA MENTRANSFORMASI ASAM KOLAT MENJADI ASAM DEOKSIKOLAT I Gusti Agung Gde Bayu Wirama; Yan Ramona; Cok Istri Sri Arisanti
Jurnal Biologi Udayana Vol 19 No 1 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Tujuan penelitian ini adalah untuk mengetahui ketahanan dari 20 isolat Lactobacillus sp., yang sebelumnya diisolasi dari susu kuda Sumbawa, terhadap pH rendah dan NaDC konsentrasi tinggi. Kemampuan isolat tersebut untuk mengubah CA ke DCA juga diamati pada penelitian ini. Hasil penelitian menunjukkan bahwa 11 strain dapat bertahan hidup dalam medium yang mengandung 0,6 mM NaDC pH 2 atau pH 3. Semua strain tahan hidup pada medium pH 4 yang mengandung 0,2 mM NaDC. Pada uji biotransformasi yang dilakukan pada 5 strain yang paling potensial berdasarkan uji ketahanan asam dan NaDC, menunjukkan bahwa tidak satupun strain-strainuji tersebut melakukan biotransformasi CA menjadi DCA, dan hal ini menunjukkan bahwa isolat-isolat tersebut berpotensi untuk dikembangkan menjadi probiotik.
KEANEKARAGAMAN KARANG KERAS DAN IKAN TERUMBU DI PANTAI PAPUMA JEMBER, JAWA TIMUR OKTIYAS MUZAKY LUTHFI; RENDY VIDYA WIBISONO
Jurnal Biologi Udayana Vol 22 No 1 (2018): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (848.572 KB) | DOI: 10.24843/JBIOUNUD.2018.v22.i01.p03

Abstract

ABSTRACT Coral reef in Papuma was distributed in 3 to 20 m depth, with sand as main substrate. The coral reef was built from hard coral (scleractinian) and reef fishes. Papuma’s coral reef was interesting to be studied due to they can deal with the extreme environment such as high sand sedimentation, high wave, strong current, and anthropogenic risk. The purpose of this study was to descript distribution of hard coral and their fishes based on quantitative data in Papuma. We used Line Intercept Transect (LIT) method with 100 m long perpendicular with coast line to quantify of coral cover based on their life form. While reef fishes data was obtained using underwater visual census with 100 m long. All data was taken from 3 stasiun they were: station 1 (8°25'59.26"S 113°33'15.07"E), station 2 (8°26'0.93"S 113°33'17.53"E) and station 3 (8°26'2.02"S 113°33'20.06"E), in 3-12 m depth. The result showed that coral reef in Papuma was dominated by branching coral (CB) from genus Montipora with coral cover 30%, while sand (SD) to be main substrate with 26.9% of coverage. Montipora is one of genus famili Acroporidae that has faster growth than massive coral and strong branch as an adaptation strategy with strong current. In this research, we also found 137 of reef fishes that was 19% composed by indicator fish (Chaetodontidae). High number of indicator fish can represent of healthy coral reef. High coral cover coverage usually will be followed by increasing number of indicator fish. These fish were obligate corallivorous that consume coral polyp during their life. Coral reef in Papuma contained 10 types of life form and had coral cover about 39.9% in average.
MOTILITAS DAN VIABILITAS SPERMATOZOA MENCIT (MUS MUSCULUS L.) SETELAH PEMBERIAN EKSTRAK TEMU PUTIH (CURCUMA ZEDOARIA (BERG.) ROSCOE.) Elfira Dzikri Ashfahani; Ngurah Intan Wiratmini; A.A.S.A Sukm aningsih
Jurnal Biologi Udayana Vol 14 No 1 (2010): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This research was aimed to study the effect of temu putih (Curcuma zedoaria (Berg.) Roscoe.) extract treatment on viability and motility of mice (Mus musculus L.) spermatozoa. The extract was given orally once a day in 35 days. The animals were divided into four groups; one control group and three treatment groups with six replicates. (M0 = control group; M1 = group was given 100 mg/Kg body weight/day; M2 = group was given 200 mg/Kg body weight/day; M3 = group was given 300 mg/Kg body weight/day). The result of the study showed that motility and viability of spermatozoa were decreased significantly (p< 0.05) after receiving temu putih (C. zedoaria (Berg.) Roscoe.) extract for 35 days.
IDENTIFIKASI LARVA SARCOPHAGIDAE (GENUS SARCOPHAGA) PADA BANGKAI MENCIT (Mus musculus) DI HUTAN MANGROVE Ayu Saka Laksmita; Ni Luh Watiniasih; I Ketut Junitha
Jurnal Biologi Udayana Vol 19 No 2 (2015): JURNAL BIOLOGI
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

Penelitian ini bertujuan untuk mengidentifikasi larva Sarcophagidae pada bangkai mencit di hutan mangrove(Mangrove Information Center, Suwung, Denpasar). Kronologi kehadiran dan tahap perkembangan serangga tersebut berkaitan dengan prediksi lama kematian untuk kepentingan forensik. Sampel dalam penelitian ini adalah semua tahap perkembangan serangga yang terdapat pada bangkai mencit sebagai model. Pengambilan sampel dilakukan di dua daerah, yaitu di daerah daratan dan perairan. Pada masing-masing lokasi diletakkan lima bangkai mencit pada tiga titik yang berbeda. Pengambilan sampel dilakukan selama lima hari berturut-turut. Identifikasi jenis dan tahap perkembangan serangga dilaksanakan di Laboratorium Taksonomi Hewan dan Laboratorium Ekologi, Jurusan Biologi, FMIPA, Universitas Udayana. Analisa data dilakukan secara deskriptif. Perbedaan perkembangan serangga dari telur sampai pupa maupun bekas pupa yang ditemukan pada bangkai dapat digunakan untuk memprediksi lama kematian dari suatu hewan.
PENGARUH PEMBERIAN RHODAMIN B TERHADAP SIKLUS ESTRUS MENCIT (Mus musculus L.) BETINA G.A.A Rina Febrina; Ngurah Intan Wiratmini; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 17 No 1 (2013): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

The research was conducted to identify the effect of rhodamine B on estrous cycle on female mice (Mus musculus L.). Twenty-four female mice were used, and randomly divided into four groups.  Rhodamine B was given orally (gavage) with a dose of  0 ppm (control), 150 ppm, 300 ppm, and 600 ppm in two estrous cycles.  Treatments were started when mice in estrus phases. The time between first and the next estrus phase were observed from vaginal smear preparation. Data obtained were analysed with One Way Anova.  If the differences were appeared between treatments, then the LSD test was performed. The results showed that rhodamine B was significantly increased the time of estrous cycle (P<0.05).

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