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JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Published by Universitas Lampung
ISSN : 14117525     EISSN : 24610399     DOI : -
Core Subject : Agriculture,
Jurnal Hama dan Penyakit Tropika (JHPT Tropika) publishes articles in plant pests, plant pathogens, plant damage caused by those pests and pathogens and or their management in tropical areas. In addition to basic and applied research papers, JHPT Tropika publishes short communication that have not been published. Before being accepted for publication, all manuscripts must be peer reviewed. The journal is published sixmonthly in March and September.
Arjuna Subject : -
Articles 521 Documents
Efficacy of powder formulations of some tropical flora and clay against Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) on maize in storage Kingsley Chidi Emeasor; Udeme Etim Jonah; David Nwazuo Enyiukwu
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22316-23

Abstract

A study was carried out to determine the efficacy of three plant materials; pawpaw (Carica papaya) seed powder, kapok tree (Ceiba pentandra) leaf powder, soursop (Annona muricata) leaf powder as well as clay and Permethrin dust against Sitophilus zeamais (Mots.) under laboratory conditions. The experiment was laid out in 5×4 factorial with 5 replicates in Completely Randomized Design in the laboratory. Maize grains weighing 50 g were treated with the plant powders, clay and the synthetic Permethrin at the rate of 0.00 g, 1.5 g, 3.5 g and 5.0 g by weight and then infested with 10 adult weevils in each vial for assessments of adult mortality, F1 emergence and damage. The comparative effectiveness of each of the treatments were assessed by counting weevils’ mortality at 14 days, 28 days, 42 days and 56 days post treatment and progeny emergence at 28 days, 42 days and 56 days post-treatment. The results were subjected to statistical analysis after transforming the data using mean percentage method and the ANOVA, the significant means were separated using the least significant differences test at 5% level. The Permethrin, a synthetic insecticide proved to be very effective followed by clay among all the treatments. Among the botanicals, C. papaya was the most effective followed by A. muricata, but were concentration dependent while C. pentandra was the least or not effective. In all trials, percentage mortality among adult S. zeamais reared on maize treated with Permethrin powders was observed to be the highest (100%) followed by those treated with C. papaya seed powder (56.6%), clay (55.5%) and A. muricata seed powder (38.8%). However, grains treated with powdered leaves of C. pentandra exhibited a low mean percentage mortality of 0.00, 0.20, 0.57 and 0.63, respectively, at different intervals post treatment which was not significantly different when compared with the untreated (control). These percentage mortality among adults were significantly (P<0.05) different from the untreated (control). Thus, all the treatments were found to significantly affect the survival of the S. zeamais at different concentration except in C. pentandra. Effect of plant powder on the emergence of adult S. zeamais was significantly (P< 0.05) different and reduced the F1 progeny emergence. The result indicated that C. pentandra had no significant effect on mortality and F1 emergence of adult S. zeamais (P< 0.05) resulting in severe damage of the grains and weight loss.
Hemolysis and hypersensitive tests ease culture collection management of antagonistic bacteria Amaria, Widi; Meity Suradji Sinaga; Kikin Hamzah Mutaqin; Supriadi; Widodo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22324-30

Abstract

A biosafety assessment is a crucial first step in the management of microbial culture collection to screen and determine unexpected potential plant and human pathogenic bacteria. It is common to collect and store as many fresh culture collections from natural resources before being further evaluated for antagonist bacteria. As a result, a bulk of isolates must be preserved which required more effort and budget. Safety evaluations based on the hemolysis and hypersensitive reactions offer simple tests to ease culture collection management of antagonist bacteria. The study aimed to evaluate the safety of bacterial culture collection for their hemolysis and hypersensitive reactions. Ninety-five isolates of rhizosphere and endophytic bacterial isolates from the culture collections of the Department of Plant Protection-IPB University, Indonesian Industrial and Beverages Crops Research Institute, and Indonesian Spice and Medicinal Crops Research Institute, were evaluated their safety using the hemolysis and hypersensitive tests. The hemolysis test was conducted using blood agar media, from which isolates with a negative (?) reaction were then tested for the hypersensitivity reaction on tobacco leaves. Bacterial isolates passed from both hemolysis and hypersensitivity tests were then preserved by the lyophilization method for long-term storage of culture collection. Based on the hemolysis test, 68 out of 95 bacterial isolates (71.57%) were found to be positive (? or ?) reactions. The hypersensitive test showed that 22 of 27 negative hemolysis isolates did not trigger hypersensitivity reactions in tobacco leaves, therefore, they were preserved by lyophilization. The study indicated that a high number of bacterial isolates in the present collection, 68 positive hemolysis, and 5 hypersensitive, need to be re-evaluated due to their safety concerns. The present study highlights the importance of biosafety tests performed in an early stage before the to permanent collection of antagonist isolates.
Preservation of weeds’ pathogenic fungi in tempeh and tapioca liquid waste and its effectiveness in goatweed (Ageratum conyzoides) Soesanto, Loekas; Mugiastuti, Endang; Ragil Sastyawan, Murti Wisnu; Manan, Abdul
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22331-41

Abstract

This research aimed to determine the best liquid media for the propagation of weed pathogenic fungi, the duration of the fungus storage on the media, and their virulence on goatweed (Ageratum conyzoides). The research consisted of two stages, i.e., the propagation of weed pathogenic fungi in alternative liquid media using a factorial completely randomized design, with the first factor being the pathogenic weed fungus (Curvularia sp., Fusarium sp., and Chaetomium sp.) and the second one being the media (tempeh or tapioca liquid waste) with four replicates. Applications were carried out using a hand sprayer on the underside of weed leaves at a density of 106 conidia or cfu mL-1. Each weed was sprayed with 10 mL of the solution. Variables observed were conidia density, number of colonies, incubation period and symptoms, disease intensity, the area under the disease progress curve (AUDPC), plant height, number of leaves, dry crown weight, and dry root weight. The results showed that tempeh and tapioca liquid waste media could be used as alternative media for multiplying pathogenic fungi. The conidia density of Curvularia sp. was 2.375 × 109 conidia mL-1 higher than that of Fusarium sp. at 1.7 × 109 conidia mL-1 and Chaetomium sp. at 9.5×104 cfu mL-1. Curvularia sp. propagated in tempeh liquid waste was able to cause damage to the leaves of goatweed as shown successively from the incubation period of 3.33 dai or accelerating 81.50%, increasing the disease intensity of 88.78%, and the AUDPC of 713.25% days compared to control. The most effective shelf life of Curvularia sp., Fusarium sp., and Chaetomium sp. in both tempeh and tapioca liquid waste media was found at six weeks at room temperature. Curvularia sp. in tapioca liquid waste could decrease weed height, the number of leaves, shoot dry weight, and root dry weight by 45.11, 28.65, 22.12, and 46.25%, respectively, compared to control.
The role of bacterial consortium as bioactivator to stimulate production and suppress grain rot disease and bacterial leaf blight in rice Sahlan, Fadyah Khamila; Ayu Eka, Nur Fadhillah; Junaid, Muhammad; Hardina, Nur; Nurmujahidin; Kuswinanti, Tutik; Patandjengi, Baharuddin
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22365-70

Abstract

Burkholderia glumae and Xanthomonas oryzae pv. oryzae are the primary causes of bacterial disease in rice, capable of reducing production and posing a threat to food availability. This research aims to assess the efficacy of a consortium of five bacteria contained in Mikrobat as bioactivators. The goal is to enhance rice yields, mitigate the severity, and suppress grain rot disease and bacterial leaf blight in rice. The research employed a split-plot design encompassing 12 treatment combinations replicated three times. The experimental setup involved two factors: the main plot consisted of rice varieties-Ciherang, Inpari 4, and Trisakti-while the subplots represented types of bioactivators-Mikrobat, EM4, Trichoderma asperellum, and a control. Results demonstrated Mikrobat’s positive impact compared to the control, with an average of 17.37 productive tillers and higher yields than other bioactivator treatments. Notably, Mikrobat exhibited 50.04% spikelets per panicle and a 1000-grain weight of 19.77 g. The severity of disease with Mikrobat treatment was notably lower than with EM4 and T. asperellum, featuring 4.20% for grain rot disease and 4.41% for bacterial leaf blight. This was significantly different from the control, which showed severity rates of 9.94% for grain rot disease and 7.75% for bacterial leaf blight. Among the three tested varieties, Ciherang demonstrated the best response, displaying higher resistance to both tested diseases. The varietal treatments did not significantly differ from each other. Although Mikrobat’s effects were not significantly distinct from EM4 and T. asperellum, its usage still reduced the severity and suppression of grain rot and leaf blight diseases
The toxicity of ammonia as a fumigant to dry wood termite (Cryptotermes cynocephalus L.) on sengon wood Irfan, Abdul Mubaraq; Sakti Harahap, Idham; Pudjianto
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22342-48

Abstract

Wood-based packaging material is usually made from low-quality kind of wood, making it a potential medium for carrying or housing wood-destructive organisms. The objective of this research was to investigate the toxicity of ammonia as a fumigant for controlling the dry-wood termite, Cryptotermes cynocephalus. Ammonia was applied as ammonium hydroxide (NH4OH) 25%, which was placed inside the fumigation chamber. At 2.25 cm wood thickness, toxicity tests were performed using 3 levels of NH4OH doses (0, 700, and 3800 mL/m³) and 4 levels of exposure (4, 6, 8, and 10 hours). The toxicity of ammonia fumigant at 1.25 cm wood thickness was assessed using 6 dose levels of NH4OH (0, 200, 378, 587, 1732, and 4188 mL/m³) for 4 hours of exposure. The LD50 and LD90 values of ammonia against the dry-wood termite, C. cynocephalus, inside the sengon wood at 2.25 cm thickness after 4 hours of exposure were 3263 mL NH4OH/m³ and 22,781 mL NH4OH/m³, respectively. Moreover, for 1.25 cm wood thickness, for the same exposure duration (4 hours), the LD50 and LD90 values of ammonia fumigant were 541.594 mL NH4OH/m³ and 1432.125 mL NH4OH/m³, respectively. Meanwhile, for 0.25 cm wood thickness, the LD50 and LD90 values were 67.67 mL of NH4OH/m³ and 241.140 mL NH4OH/m³, respectively. In addition, for 2.25 cm wood thickness, the LD50 and LD90 values of ammonia fumigant were lower when the exposure time was longer (2149 and 10,722 mL NH4OH/m³ for 6 hours of exposure, and 1373 and 8705 mL NH4OH/m³ for 8 hours of exposure).
Effects of holy basil (Ocimum sanctum) on viral disease of chili (Capsicum annum L.) under mixed crop cultivation Arsi, Arsi; SHK, Suparman; Lailaturrahmi; Hamidson, Harman; Pujiastuti, Yulia; Umayah, Abu; Gunawan, Bambang; Pratama, Rahmat; Irsan, Chandra; Suwandi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22349-57

Abstract

Chili is one of the important commodities in Indonesia. Chili cultivation faces many obstacles, leading to a decrease in production, such as virus infections. Yellow leaf curl and curly top viruses are groups of viruses that are often found in chili fields. This research was conducted to investigate the effect of intercropping (basil-chili) in suppressing viral diseases, especially yellow leaf curl and curly top viruses. A Randomized block design was used for this research, including intercropping and monoculture. The parameters observed in this study included disease incidence and intensity, the number and weight of chilies. Symptoms of virus infection include leaf curl, yellowing, and stunting. The disease has been reported to be transmitted by insect vectors, such as Aphis gossypii and Bemisia tabaci. The results showed that the incidence and intensity of yellow leaf curl and curly top virus diseases in chili pepper and basil plants were not significantly different in each treatment. The number of healthy chilies and their weight were also not significantly different. Damaged chilies are fruits that have been attacked by fruit flies. Basil intercropped with chilies attracts fruit flies, which affects the quantity and weight of the harvested chilies. The population of B. tabaci was significantly different in each treatment, while A. gossypii did not show significant differences among the treatments. Intercropping basil and chili increased the population of A. gossypii, while the population of B. tabaci decreased in this treatment. These two insect pests are vectors for yellow leaf curl and curly top viruses.
Joint application of B. bassiana and M. anisopliae bioinsecticides for controlling rice bugs and improving rice yields Ponijan; Handayani, Etik Puji; Kurniawati, Nurleni; Rakhmiati; Zulkarnaen
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22358-64

Abstract

The rice bug (Leptocorisa oratorius F.) is a serious pest of head rice (Oryza sativa L.). It feeds on the developing kernels of rice, starting from the heading phase when the panicle is exposed from the boot until the end of the ripening phase, which makes it susceptible to indirect yield loss. Multiple insecticide applications are often made during the hard drought growth stage due to high densities of rice bugs that frequently migrate from nearby harvested fields or as a result of eggs laid by subthreshold populations during the first two weeks of heading. The research was conducted at The Food Crops and Horticulture Laboratory Trimurjo, Central Lampung, from August to October 2020, using a randomized complete block design (RCBD) with 7 bioinsecticide treatments. The objective of the research was to determine the efficacy of integrating biopesticides B. bassiana and M. anisopliae on rice bug mortality and rice yields. The results showed that rice bug mortality increased by 100% with a lethal period of 72 hours after exposure to B. bassiana 5 g L-1 + M. anisopliae 5 g L-1. Additionally, there was a 19.73% increase in the percentage of rice grain maturity (hard dough) and a reduction of 99.4% in damage (empty grain) compared to the control.
Effect of genetic modified maize contained Cry1Ab gene on the arthropods abundance and diversity in limited test field Herlina, Lina; Istiaji, Bonjok
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 2 (2023): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.22371-82

Abstract

In Indonesia, transgenic products are still considered innovative, and genetically modified (GM) maize has stayed on the market while its impact on environmental biosafety is now being evaluated. Bt corn has been recognized as one of the solutions to the problem of Asian corn and cob borers to preserve maize production. Therefore, this study aimed to investigate the effect of Bt corn on arthropod richness and diversity in a limited testing field using a randomized block design with four different maize varieties as treatments in six replications. The significant findings showed that Bt corn had no significant influence on the diversity, evenness, and abundance index (Margalef and Meinhinick Index) in the limited testing fields, which were all greater at 85 days after plant (DAP) than 60 DAP. Based on the evenness and abundance index, we conclude that Bt corn does not harm the community of existing arthropods.
Morphological and molecular identification as well pathogenicity of the causal agents of fruit rot disease of apple manalagi (Malus sylvestris) in Pujon, East Java Unun Triasih; Abadi, Abdul Latief; Muhibbudin, Anton; Widyaningsih, Sri
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12356-66

Abstract

Apple fruit rot disease is one of the major apple diseases in Indonesia. It has been caused by several species of pathogenic within the genus Colletotrichum. This research aims to identify species of the pathogen causing apple fruit rot disease in Pujon, East Java. Suspected fungal isolates were identified based on morphological (macroscopic and microscopic) and molecular characteristics. Based on the morphological observation, five isolates were identified as Colletotrichum spp. These isolates have similar morphological characteristics such as white-greyish colony color, texture colony velvety, zonation conidia concentric, round cylindrical conidial end, and conidia with 10.4–12.8 ?m in length and width 3.1–3.52 ?m, respectively. Isolates M1 showed the highest pathogenicity, therefore selected for molecular identification. Molecular identification was conducted using ITS1 and ITS4 primer, AM1 was identified as C. gloeosporioides with 99.57% similarity to C. gloeosporioides JX-19 variety from China.
Enrichment of organic material with Trichoderma asperellum for the management of twisted disease on shallot Ilma, Hurin Nabila Aghnia; Wibowo, Arif; Joko, Tri; Subandiyah, Siti; Harper, Stephen
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12428-37

Abstract

Shallots are a type of plant used as a flavoring agent and are often affected by twisted disease caused by Fusarium spp. This study aims to determine the ability of organic material enriched by Trichoderma asperellum in controlling twisted disease, increasing production, and suppressing the population of Fusarium spp. in the soil. The research showed that compost and chicken manure enriched with T. asperellum in a ratio (200:1) were able to reduce the disease incidence compared to controls, in addition to increasing production and suppressing the population of Fusarium spp. in the soil. The colonies of Fusarium spp. in the soil were correlated with disease incidence in each treatment. The highest incidence of twisted disease was followed by the number of Fusarium spp. colonies. The microbial composition did not affect disease suppression when observed using a dependent method using PCR-RISA. The microbial composition with the dependent method using PCR RISA did not affect the suppression of the twisted disease in the shallot plants.

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