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All Journal International Journal of Public Health Science (IJPHS) Jurnal Riset Kesehatan Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Penelitian Pendidikan IPA (JPPIPA) Manuju : Malahayati Nursing Journal Journals of Ners Community JURNAL PENELITIAN KESEHATAN JURNAL ANALIS LABORATORIUM MEDIK Medicra (Journal of Medical Laboratory Science/Technology) JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Jurnal EduHealth Sewagati Analis Kesehatan Sains (e-Journal) Jurnal Rakat Sehat : Pengabdian Kepada Masyarakat Jurnal Sains Student Research Jurnal Laboratorium Khatulistiwa Jurnal Pengabdian Kesehatan Beguai Jejama Frontiers in Community Service and Empowerment
., Pestariati
Poltekkes Kemenkes Surabaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Pour Plate dan Spread Plate Sebagai Metode yang Akurat dalam Pemeriksaan Jumlah Bakteri dalam Urin pada Suhu Ruang dan Suhu Kulkas A'yunin, Zahratin Nisa'; Pestariati, Pestariati; Endarini, Lully Hanni; Krihariyani, Dwi
Jurnal Penelitian Kesehatan SUARA FORIKES Vol 16, No 2 (2025): April-Juni 2025 (up coming)
Publisher : FORIKES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/sf.v16i1.5012

Abstract

in process
Analisis Bakteri Methicillin Resistant Staphylococcus aureus (MRSA) pada Pus Infeksi Nosokomial Pasien Pasca Operasi Rina Idawati; Suliati Suliati; Lully Hanni Endarini; Pestariati Pestariati
JOURNAL SAINS STUDENT RESEARCH Vol. 3 No. 4 (2025): Jurnal Sains Student Research (JSSR) Agustus
Publisher : CV. KAMPUS AKADEMIK PUBLISING

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61722/jssr.v3i4.5984

Abstract

Nosocomial infections are a serious concern in healthcare services, particularly among postoperative patients. One of the main pathogens responsible for nosocomial infections is Staphylococcus aureus, especially strains resistant to the antibiotic methicillin (MRSA). MRSA infections can worsen patient conditions, prolong hospital stays, and increase medical costs.This study aims to analyze the presence of MRSA bacteria in pus samples from nosocomial infections in postoperative patients and to determine its prevalence at General Hospital.This research employed a descriptive-analytic observational method using both primary and secondary data from postoperative patients with nosocomial infections. Pus samples were cultured and identified through Gram staining, catalase test, coagulase test, biochemical tests, and antibiotic susceptibility testing using the Microbiology Analyzer TDR-300B Mindray. The study was conducted from August to November 2024.From 41 pus samples collected from postoperative patients with nosocomial infections, 6 samples tested positive for MRSA, indicating a prevalence of 14.6%. Antibiotic susceptibility tests showed resistance patterns to several beta-lactam antibiotic classes.
Pemanfaatan Teri Jengki (Stolephorus indicus) Dan Layang Deles (Decapterus macrosoma) Sebagai Media Alternatif Pertumbuhan Escherichia coli Nurhasanah, Armelia Gitasari; Pestariati, Pestariati; Anggraini, Anita Dwi; Astuti, Sri Sulami Endah
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 1 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i1.14452

Abstract

ABSTRAK Nutrient agar merupakan jenis media umum yang sering digunakan pada laboratorium, harga media ini relatif mahal. Nutrient agar berbentuk padat, terdiri atas campuran ekstrak daging, pepton serta agar. Ikan teri jengki dan ikan layang deles jumlahnya melimpah di Indonesia. Ikan teri jengki (Stolephorus indicus) mengandung protein 51.54%, sementara ikan layang deles (Decapterus Macrosoma) mengandung protein sebesar 57,89%. Escherichia coli digunakan sebab bakteri ini merupakan salah satu jenis mikroorganisme kontrol positif yang direkomendasikan berdasarkan Data Sheet Nutrient Agar OXOID. Penelitian ini bersifat eksperimental dengan tiga perlakuan pada masing-masing bahan yaitu variasi massa 3 gram, 4 gram dan 5 gram yang dilakukan di Laboratorium Bakteriologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya. Hasil penelitian menunjukkan jumlah koloni rata-rata Escherichia coli yang tumbuh pada kontrol positif sebanyak 118 x 1013, sementara pada media ikan teri jengki variasi massa 3 gram adalah 72 x 1013 CFU/mL, 4 gram sebanyak 85 x 1013 CFU/mL, 5 gram sebanyak 94 x 1013 CFU/mL. Pada media ikan layang deles variasi 3 gram sebanyak 88 x 1013 CFU/mL, 4 gram sebanyak 96 x 1013 CFU/mL, 5 gram 108 x 1013 CFU/mL. Variasi massa yang paling baik dan mendekati hasil kontrol positif (nutriet agar) pada ikan teri jengki dan ikan layang deles adalah variasi massa 5 gram.Kata kunci: ikan teri jengki, ikan layang deles, Escherichia coli, nutrient agar
Studi Komparasi Hasil Pemeriksaan Mycobacterium Tuberculosis Pada Pasien Suspek Tuberkulosis Dengan Menggunakan Tes Cepat Molekuler dan Mikroskopis di RSUD Karangasem Wicaksana, I Nyoman Krisna; Pestariati, Pestariati; Arifin, Syamsul
Journals of Ners Community Vol 13 No 1 (2022): Journals of Ners Community
Publisher : Fakultas Ilmu Kesehatan Universitas Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55129/jnerscommunity.v13i1.1746

Abstract

Tuberkulosis merupakan suatu penyakit kronik menular yang disebabkan oleh Mycobacterium tuberculosis yang menyebabkan jumlah kematian global mencapai 1.3 juta pada tahun 2020 dan ditambah parah setelah adanya pandemi COVID-19 yang mengakibatkan terbatasnya layanan tuberkulosis, kasus di Indonesia selalu mengalami peningkatan tiap tahunnya, tahun 2019 ditemukan sebanyak 543.874 kasus meningkat dari 420.994 kasus di tahun 2017. Metode pemeriksaan yang banyak digunakan di negara endemik tuberkulosis adalah Mikroskopis, namun terdapat beberapa kekurangan dalam penggunaanya dibandingkan dengan metode Tes Cepat Molekuler. Penelitian ini bersifat observasional dengan pendekatan cross sectional yang bertujuan untuk mengetahui perbedaan hasil pemeriksaan Mycobacterium tuberculosis pada pasien suspek tuberkulosis dengan menggunakan Tes Cepat Molekuler dan Mikroskopis di RSUD Karangasem. Penelitian ini dilaksanakan dari bulan Desember 2021 sampai April 2022 menggunakan sampel sputum yang diperoleh dari RSUD Karangasem. Penelitian menunjukan adanya perbedaan hasil sensitivitas dan spesifisitas pada dua metode. Sensitivitas metode Tes Cepat Molekuler lebih tinggi daripada metode Mikroskopis (100% dan 75%) sedangkan nilai spesifisitas metode Mikroskopis lebih tinggi daripada metode Tes Cepat Molekuler (100% dan 95%).
Efforts to Control Blood Pressure for Prolanis Hypertension Members through the Utilization of Bay Leaf Decoction Handayati, Anik; Suhariyadi; Pestariati
Frontiers in Community Service and Empowerment Vol. 4 No. 3 (2025): September
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ficse.v4i3.104

Abstract

Bay leaves are a type of kitchen spice that is often added to dishes, inexpensive, and easily obtained. Research results have proven that the active compounds in bay leaves are quite effective and safer in lowering blood pressure. Most of the Sreseh residents work as fishermen who tend to consume salty foods, which causes a high incidence of hypertension. Therefore, it is necessary to increase knowledge about healthy lifestyles and the use of herbal medicine made from boiled bay leaves to lower blood pressure among Prolanis hypertension members in the Sreseh Health Center area. The methods used in this community service activity are lecture/presentation and demonstration methods. Lecture activities are carried out by delivering material on the prevention and natural treatment of hypertension using bay leaves, the benefits of bay leaves, and the processing of bay leaves for hypertension treatment. The demonstration method is carried out by showing how to process bay leaves. The target of the activity is Prolanis hypertension patients in Noreh Village, within the working area of the Sreseh Health Center, Sampang, Madura. Members of the Chronic Disease Management Program (PROLANIS), especially those with hypertension, were very enthusiastic and motivated to participate in every stage of the community service activity, from blood pressure checks, Prolanis exercises, to education on the benefits of bay leaves. After consuming boiled bay leaves, blood pressure checks on Prolanis members with hypertension showed a 30% decrease in the number of Prolanis members with hypertension, from the previous 76.67% to 46.67%.
Development of a Rapid Diagnostic Method for Simultaneous Detection of Streptococcus viridans in Cases of Heart Disease Pestariati, Pestariati; Suhariyadi, Suhariyadi; Asryadin, Asryadin; Yuniati, Nilasari Indah
Jurnal Penelitian Pendidikan IPA Vol 11 No 11 (2025): November: In Progress
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i11.12730

Abstract

Heart disease is a leading cause of death worldwide, with a complex pathophysiology often involving interactions between genetic factors, the environment, and pathogenic microorganisms. Streptococcus viridans is a clinically significant pathogenic bacterium associated with infections of the cardiovascular system, including infective endocarditis, pericarditis, and other complications. However, timely and accurate diagnosis of this bacterial infection in cases of acute heart disease is often challenging, requiring rapid and sensitive diagnostic methods. Currently, diagnostic methods for detecting Streptococcus viridans in cases of acute heart disease tend to be time-consuming; therefore, developing a rapid diagnostic method that can detect both bacteria simultaneously is crucial. The aim of this study is to develop a rapid and sensitive diagnostic method for detecting the presence of Streptococcus viridans in samples from patients with heart disease. The method used is the identification of specific genes, the design of primer sequences, and the design of probes using specific 16s rRNA genes using bioinformatics techniques. Based on the research results obtained primer pair sequences are: oligonucleotide primer forward 5’-GCGACGATACATAGCCGAC-3’; primer reverse is 3’- CGAGCCAGTCTGAAAGC-5’, while the probe sequence is 5’-GACTGAGACACGGCCCAGACTC-3’. Primer and probe pair quality tests showed very good primer and probe quality for amplification with a 120 bp amplification product. Suggestions in the research are that it is necessary to continue with qPCR optimization to determine the melting temperature which is then carried out sensitivity tests of primer pair sequences and specific 16s rRNA Streptococcus viridans gene probes.
PERBANDINGAN PEMERIKSAAN TELUR Soil-Transmitted Helminth DENGAN SEDIMENTASI NaCl 0,9% dan NaOH 0,2%, SERTA FLOTASI NaCl JENUH Sania, Zahrotus; Pestariati, Pestariati; Arifin, Syamsul
Jurnal Analis Laboratorium Medik Vol 10 No 2 (2025): Jurnal Analis Laboratorium Medik
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v10i2.6165

Abstract

Latar belakang: Infeksi Soil-Transmitted Helminth (STH) menjadi salah satu persoalan kesehatan masyarakat yang lazim ditemui, terutama di daerah tropis dan subtropis dengan sanitasi yang buruk. Infeksi ini bereakibat pada gangguan kesehatan, misalnya anemia, hambatan tumbuh kembang, hingga penurunan imunitas tubuh. Oleh karena itu, deteksi dini infeksi kecacingan sangat penting dilakukan untuk menunjang diagnosis dan pengendalian penyakit. Salah satu uji yang umum digunakan adalah pemeriksaan tinja secara mikroskopis, yang efisiensinya sangat bergantung pada teknik yang digunakan. Tujuan: untuk menganalisis komparasi hasil pemeriksaan tinja menggunakan metode sedimentasi NaCl 0,9%, sedimentasi NaOH 0,2%, dan flotasi NaCl jenuh dalam mendeteksi telur cacing Soil-Transmitted Helminth. Metode: eksperimental berbasis laboratorium dengan pendekatan komparatif. Sampel tinja yang telah dipastikan positif STH diperiksa menggunakan ketiga metode secara sistematis, dengan pengujian sebanyak tiga kali untuk masing-masing metode. Analisis data dilakukan menggunakan uji normalitas Shapiro-Wilk, uji homogenitas, uji ANOVA One-Way, dan uji Post Hoc. Hasil: metode sedimentasi NaCl 0,9% menghasilkan rerata jumlah telur tertinggi (9,33), diikuti NaOH 0,2% (8,33), dan flotasi NaCl jenuh (4,00). Terdapat perbedaan signifikan antar metode (p = 0,007), dengan perbedaan signifikan antara metode flotasi dan kedua metode sedimentasi. Kesimpulan: bahwa metode sedimentasi NaCl 0,9% merupakan metode paling efektif dalam mendeteksi telur STH pada penelitian ini.
Deteksi Gen blaZ pada Staphylococcus aureus Isolat Urine Pasien ISK Menggunakan Metode Real-Time PCR Verdiani, Intan; Pestariati, Pestariati; Puspitasari, Ayu
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2084

Abstract

Urinary tract infection (UTI) is one of the most common bacterial infections and is often caused by various types of bacteria. In recent years, the prevalence of Staphylococcus aureus in UTI cases has increased, along with rising resistance to antibiotics, particularly the β-lactam group such as penicillin. This study aimed to detect the blaZ gene, which plays a role in penicillin resistance in S. aureus isolated from the urine of UTI patients. The study was conducted from November 2024 to May 2025 using a descriptive quantitative design. A total of 100 urine samples from UTI patients were obtained from RSPAL Dr. Ramelan Surabaya. Bacterial identification was carried out at the Microbiology Laboratory of RSPAL Dr. Ramelan using the Vitek 2 Compact system. Phenotypic resistance testing against penicillin G was performed at the Bacteriology Laboratory of the Department of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya using the Kirby-Bauer disc diffusion method. Genotypic detection of the blaZ gene was conducted using the Real-Time PCR method at the Molecular Biology Laboratory. The results showed that out of five Staphylococcus aureus isolates phenotypically resistant to penicillin G, only three isolates (60%) were positive for the blaZ gene. These findings indicate that penicillin resistance in S. aureus is not solely mediated by the blaZ gene and highlight the importance of molecular surveillance of β-lactam resistance genes in clinical isolates.
Detection of The Blaz Gene as a Penicillin Resistance Marker in Staphylococcus Aureus From Diabetic Ulcer Swabs Indrayani, Salsabila Anggia; Pestariati, Pestariati; Astuti, Sri Sulami Endah
Jurnal Riset Kesehatan Vol 14 No 2 (2025): NOVEMBER 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jrk.v14i2.13592

Abstract

Diabetic ulcer is the most common complication in patients with diabetes mellitus, and Staphylococcus aureus is the predominant bacterial cause of infection. The aim of this study was to detect the presence of the blaZ gene as a penicillin-resistance marker in S. aureus isolated from diabetic ulcer swabs. This research was a descriptive quantitative study. The study population consisted of patients with diabetic ulcers treated at the Diabetes Wound Care Center Branch in Surabaya. Respondents (samples) were selected using purposive sampling, with inclusion criteria being patients diagnosed with diabetic ulcers and eligible for swab collection. A total of 30 ulcer swab samples were obtained between April and May 2025. Isolation and identification were conducted at the Bacteriology Laboratory of Poltekkes Surabaya, resulting in 16 isolates (54%) identified as S. aureus. Among these, 10 isolates (62.5%) were resistant to penicillin G. Detection of the blaZ gene using Real-Time PCR at the Molecular Biology Laboratory of Poltekkes Surabaya showed that 8 isolates (80%) were positive for the gene. These findings indicate that most penicillin-resistant S. aureus isolates carry the blaZ gene, suggesting high potential for β-lactamase production. Future studies are recommended to include larger sample sizes, analyze additional resistance genes, and investigate clinical factors influencing the emergence of antibiotic resistance in diabetic ulcers.
Co-Authors A'yunin, Zahratin Nisa' Anggraini, Anita Dwi Anindya, Lokawanti Al Fahna Anita Dwi Anggraini Anita Dwi Anggraini Ariana, Novi Armelia Gitasari Nurhasanah Asryadin, Asryadin Ayu Puspitasari, Ayu Christ Kartika Rahayuningsih Dawami, Tamymatur Roudhatud Diah Titik Mutiarawati Edy Haryanto Endah Astuti, Sri Sulami Endarini, Lully Hanni Evy Diah Woelansari Gufron, Gusti Naila Rachmawati Handayati, Anik I Gusti Bagus Wiksuana I Nyoman Krisna Wicaksana Ida Lestari Iis Rofiatul Ana Indrayani, Salsabila Anggia Istanto , Wisnu Istanto, Wisnu Juliana Christyaningsih Krihariyani, Dwi Kumala, Nyna Rachma Kusumaningrum, Debby Liliek Soetjiatie Luthfi Rusyadi Mamik Mamik Mamik Mamik Meilinda Rachmawati Minarti Minarti Minarti Minarti Mufidah Mufidah Muhammad Sholakhuddin Al-Ayubi Museyaroh, Museyaroh Mutiarawati, Diah Titik Nurhasanah, Armelia Gitasari Nyna Rachma Kumala Retno Sasongkowati Rina Idawati Sania, Zahrotus Sasongkowati, Retno Sri Sulami Endah Astuti Sri Utami Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Syamsul Arifin Syamsul Arifin Verdiani, Intan Wicaksana, I Nyoman Krisna Wisnu Istanto Wisnu Istanto Wisnu, Istanto Yuniati, Nilasari Indah