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Journal : Indonesian Aquaculture Journal

IN VITRO ANTIBACTERIAL EFFICACY OF LEAVES EXTRACT OF Centela asiatica AGAINST Vibrio harveyi AND Aeromonas hydrophila Rukisah Rukisah; Diana Maulianawati; Jimmy Cahyadi
Indonesian Aquaculture Journal Vol 14, No 2 (2019): (December, 2019)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (712.231 KB) | DOI: 10.15578/iaj.14.2.2019.69-74

Abstract

Disease infection is one of the limiting factors that affect productivity in aquaculture and has caused economic losses. Luminescent vibrios and motile aeromonas septicemia (MAS) are diseases caused by Vibrio harveyi and Aeromonas hydrophila bacteria, respectively. Certain plants have antimicrobial compounds and can potentially be used to treat the diseases, such as Centella asiatica. In the present study, the crude leaves extracts of C. asiatica were examined for its antibacterial potential using methanol solvents against V. harveyi and A. hydrophila bacteria. Different concentrations of 50 mg/mL and 100 mg/mL were checked for its antibacterial activity. The crude extract was also tested for phytochemistry content and LC50 using Brine Shrimp Lethal Assay. The crude extracts of C. asiatica showed a remarkable antibacterial activity with inhibition zone of 10.57 mm against A. hydrophila and 21.14 mm against V. harveyi. The phytochemistry test result showed that C. asiatica leaves contain alkaloid, phenol, and tannin compounds. The acute lethal concentration (LC50) of C. asiatica after 24 hours exposure to the extract mixture was 254 mg/L. The results confirmed the potential use of C. asiatica extracts as a source of antibacterial compounds.
Co-Authors Abdul Jabarsyah Abdul Muis Prasetia, Abdul Muis Agus Indarjo Agus Indarjo Agus Indarjo Ahmad Arsyidi Anhar Rozi Anugrah, Wulan Arsyidi, Ahmad Asniar Asniar Asriadi Asriadi Aswar Gunawan Awaludin Awaludin Awaludin Awaludin Awaludin Awaludin, Awaludin Awaludin, Awaludin Ayu Tri Fatma Ayu Tri Fatma Azis - Hamzah Burhanuddin Ihsan Burhanuddin Ihsan Burhanuddin Ihsan, Burhanuddin cahyadi, jimmy Christine Dyta Nugraeni Dhimas Wiharyanto Gazali Salim Gloria Ika Satriani GS , Achmad Daengs Gusman, Ery Harinto Harinto Hariyadi Hariyadi Helman Helman Heppi Iromo Ika Satriani, Gloria Ira Maya Abdiani Ira Maya Abdiani Ira Maya Abdiani Iswan, Muhammad Jabaruddin Jabaruddin Kartina Kartina Kartina Kartina Khaer, Miftahul Laurha Annisa Linting Maulianawati, Diana Miska Sanda Lembang Mohammad Fadnan Akhmadi Mufrida Zein Muhammad Amien Muhammad Firdaus Muhammad Firdaus Muhammad Iswan Muhammad Iswan Guntur Nabila Nabila Nasrul Ngungut Novi Luthfiyana Nugraeni, Christine Dyta Nurazira Nurazira Nuril Farizah Nuril Farizah Nursia Nursia Nursia, Nursia Patrice Sevania Permana Ari Soejarwo Prakoso, Lukman Yudho Putri Wening Ratrinia Randi Wahyu Andila Rasyid, Rosmawati Rezkyana Rezkyana Ricky Febrinaldy Ridwan Shabir Risman Risman Rozi Rusli Santria Santria Simanjuntak, Ricky Febrinaldy Siti Hawa Stephanie Bija Sukardi Sukardi Sukri Faldin Suriyanti Suriyanti Tamrin Toha Taufik Hidayat Thonas Indra Maryanto Tri Paus Hasiholan Hutapea, Tri Paus Hasiholan Yen Thi Hong Pham Yulma, Yulma Zainuddin Zainuddin