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Journal : Jurnal Katalisator

The BATANG KEMUMU (Colacasia gigantea cv) SEBAGAI BAHAN BAKU OBAT ALAMI ANTIBAKTERI DAN ANTIKANKER Hesti Marliza; Trie Yuni Elfasyari Elfasyari; Faziyana; Sarina Sembiring Milala
JURNAL KATALISATOR Vol. 6 No. 1 (2021): jurnal Katalisator Volume 6 No 1, 2021
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (808.831 KB) | DOI: 10.62769/katalisator.v6i1.197

Abstract

Kemumu (Colocasia gigantea cv) is a type of taro whose stems and leaves are edible. Kemumu belongs to the type of taro whose properties and phytochemical content, in particular, have not been widely reported. This study aims to determine the phytochemical content of kemumu stem as well as antibacterial bioactivity test and cytotoxic test (anticancer initial test) which can be used as raw materials for natural medicine. The stem is extracted using three types of solvents with different levels of polarity, n-Hexane, Ethyl acetate, and Ethanol. Antibacterial activity test uses agar diffusion method with some 20%, 40%, and 60% concentrations. Cytotoxic activity was tested by the Brine Shrimps Lethality Test (BSLT) method. Phytochemical test results are known that the kemumu extract contains secondary metabolites such as flavonoids, alkaloids, tannins, terpenoids, and steroids. The ethyl acetate extract contains flavonoids, tannins, terpenoids, and steroids. Antibacterial activity was found in ethyl acetate extract with a concentration of 40% with an inhibition zone of 17.5 mm. The highest LC50 value was also in the ethyl acetate extract of kemumu, which was 7.14 ppm. This value indicates a high level of toxicity so that ethyl acetate extract of kemumu acts as an antibacterial and anticancer.
The AKTIVITAS ANTIDIABETES EKSTRAK KULIT JERING (PITHECELLOBIUM JIRINGA) DENGAN FRAKSI PELARUT METANOL Alfin Surya; Dimas Prada Sumitra; Hesti Marliza; Zaiyar Zaiyar
JURNAL KATALISATOR Vol. 6 No. 1 (2021): jurnal Katalisator Volume 6 No 1, 2021
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1350.877 KB) | DOI: 10.62769/katalisator.v6i1.265

Abstract

ABSTRACT Jering skin is a solid waste that can cause problems if not treated seriously because it pollutes the environment. Based on previous research, the potential possessed by jering skin is very high because it contains flavonoids and polyphenols which are antiseptic, and antioxidant, also has a very toxic toxicity value for shrimp artemia salina, this has the potential as an anticancer. Therefore, this study aims to continue testing other activities, namely by conducting antidiabetic activity tests using the methanol solvent fraction. The method used is to use a Microplate reader or platec reader at a wavelength of 410 nm to determine absorbance, so that with data analysis the Inhibitor Concentration value of 50% (IC50) of sample extracts is inhibited by the α-glucosidase enzyme in hydrolyzing the p-nitrophenyl substrate. -α-D-glucopyranoside (p-NPG) to form glucose. The results of the study obtained an IC50 value of 387,6091 µg/mL. When compared with IC50 values ​​for positive root as a positive control of 0.8135 μg / mL, it is still weak because the root is a pure composition while the Methanol Faction still contains Rough extract still in the fractionation but the dry skin Methanol fraction can be needed as a source to be used as a substitute for further as a joint exploration of antidiabetic in jering skin. Keywords: Jering, Methanol, Antidiabeti, IC50. ABSTRAK Kulit jering merupakan limbah padat yang dapat menimbulkan masalah bila tidak ditangani dengan serius karena mencemari lingkungan. Berdasarkan penelitian sebelumnya, potensi yang dimiliki oleh kulit jering tersebut sangat tinggi karena mengandung senyawa flavonoid dan polifenol yang bersifat antiseptik, dan antioksidan, juga memiliki nilai Toksisitas yang sangat toksit terhadap udang artemia salina hal ini berpotensi sebagai antikanker. Oleh karena itu, penelitian ini bertujuan untuk melanjutkan uji aktivitas lainnya yaitu dengan melakukan uji aktivitas Antidiabetes mengunakan fraksi pelarut metanol. Adapun metode yang digunakan adalah dengan mengunakan Microplate reader atau platec reader pada panjang gelombang 410 nm untuk menentukan absorbansi, sehingga dengan analisis data diperoleh nilai Inhibitor Concentration 50% (IC50) dari ekstrak sampel dalam menghambat kerja enzim α-glukosidase dalam menghidrolisis substrat p-nitrofenil-α-D-glukopiranosida (p-NPG) untuk membentuk glukosa. Hasil dari penelitian diperoleh nilai IC50 adalah sebesar 387,6091 µg/mL. Jika dibandingkan dengan nilai IC50 untuk akarbose sebagai kontrol posistif sebesar 0,8135 µg/mL, memang masih lemah karena akarbose merupakan senyawa murni sedangkan Fraksi Metanol masih berupa ekstrak Kasar walaupun sudah di fraksinasi namun fraksi Metanol kulit jering dapat dikatakan memiliki potensi untuk dilakukan isolasi lebih lanjut sebagai eksplorasi senyawa aktif antidiabetes dalam kulit jering. Kata Kunci : Jering; Metanol; Antidiabetes; IC50.
POTENSI EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta) DALAM MENGHAMBAT PERTUMBUHAN Staphylococcus epidermidis Alfin Surya; Fitri Nadira; Hesti Marliza; Zaiyar
JURNAL KATALISATOR Vol. 6 No. 2 (2021): Jurnal Katalisator, Volume 6, No 2, 2021
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (534.191 KB) | DOI: 10.22216/katalisator.v6i2.528

Abstract

Kacapiring (Gardenia augusta) merupakan salah satu tanaman Indonesia yang berkhasiat obat. Seluruh bagian dari tanaman kacapiring dapat dimanfaatkan bagi kehidupan manusia. Daun kacapiring memiliki kemampuan sebagai antikarsinogenesis dan antimikroba. Analisis fitokimia ekstrak daun kacapiring menunjukkan kandungan melabolit sekunder yang terdapat pada ekstrak etanol daun kacapiring, diantaranya alkaloid, flavonoid, saponin, dan tanin. Penelitian ini bertujuan untuk mengetahui daya hambat ekstrak etanol daun kacapiring (Gardenia augusta) dalam menghambat pertumbuhan Staphylococcus epidermidis. Penelitian ini bersifat eksperimental laboratorium menggunakan metode difusi (Kirby & Bauer), kanamisin sebagai kontrol positif, etanol sebagai kontrol negatif dan konsentrasi 2,5%, 5%, 10% dan 20%. Berdasarkan penelitian ini didapatkan hasil daya hambat esktrak etanol daun kacapiring terhadap pertumbuhan Staphylococcus epidermidis pada konsentrasi 2,5%, 5%, 10% dan 20% adalah 6 mm, 6 mm, 6 mm, dan 6,7 mm. Maka, dari hasil penelitian ini dapat disimpulkan bahwa ekstrak etanol daun kacapiring (Gardenia augusta) pada konsentrasi 2,5%, 5%, dan 10% belum mampu menghambat pertumbuhan dari bakteri Staphylococcus epidermidis sedangkan pada konsentrasi 20% mampu menghambat pertumbuhan bakteri Staphylococcus epidermidis dengan besar rata-rata zona hambat 6,7 mm masih dikatakan dalam kategori zona hambat sedang.  
Pre-Eliminary Studi Aktivitas Sitotoksik Biota Laut Pantai Sekilak Batam Terhadap Larva Udang (Artemia salina Leach) HESTI MARLIZA; Nurliyasman Nurliyasman; RENY HARYANI; Veranica Lestari
JURNAL KATALISATOR Vol. 7 No. 1 (2022): Jurnal Katalisator Volume 7 Nomor 1, April 2022
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.466 KB) | DOI: 10.22216/katalisator.v7i1.960

Abstract

Indonesia merupakan negara kepulauan yang memiliki perairan laut yang sangat luas, serta kaya akan sumber daya alam dan keanekaragaman biota laut, salah satunya di Pantai Sekilak Batam. Biota laut memiliki berbagi jenis senyawa bioaktif, diantaranya memiliki aktivitas sebagai antikanker. Tujuan penelitian ini yaitu untuk mengetahui kandungan metabolit sekunder yang terdapat dalam biota laut yang didapat dari Pantai Sekilak Batam dan untuk mengetahui biota laut manakah yang berpotensi memiliki aktivitas sitotoksik. Sampel yang digunakan adalah empat biota laut yang berasal dari pantai Sekilak Batam. Metode penelitian menggunakan metode BSLT (Brine Shrimp Lethality Test). Hasil penelitian metabolit sekunder pada keempat sampel biota laut yang mengandung flavonoid yaitu Halimeda opuntia, Halimeda makroloba, Amphiroa fragilissima,Sinularia polydactyla. Alkaloid yaitu Halimeda opuntia, Sinularia polydactyla. Saponin yaitu Halimeda opuntia, Halimeda makroloba, dan Amphiroa fragilissima. Steroid yaitu Halimeda makroloba dan Amphiroa fragilissima, dan positif triterpenoid pada Sinularia polydactyla. Pada hasil uji aktivitas sitotoksik keempat biota laut menunjukkan hasil dengan nilai LC50 yang diperoleh dari ekstrak Halimeda opuntia sebesar 36,47 ppm Halimeda macroloba sebesar 35,48 ppm, Sinularia polydactyla sebesar 37,15 ppm, Amphiroa fragilissima sebesar 36,47 ppm. Pada hasil uji aktivitas sitotoksik keempat biota laut memiliki efek toksik dengan kategori toksik yaitu <1000 ppm.
ANALISIS KUALITATIF KANDUNGAN FORMALIN PADA IKAN ASIN DI PASAR TRADISIONAL KOTA PEKANBARU Alfin Surya; Qoirina; Hesti Marliza
JURNAL KATALISATOR Vol. 7 No. 2 (2022): jurnal Katalisator Volume 7 No 2, Oktober 2022
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (140.383 KB) | DOI: 10.62769/katalisator.v7i2.1665

Abstract

Ikan asin merupakan salah satu lauk dari ikan yang diawetkan yang sudah lama dikenal oleh masyarakat Indonesia. Untuk memperpanjang umur simpan dan memperindah tampilan produk olahan ikan, biasanya ditambahkan pengawet. Salah satu pengawet yang dicurigai ditambahkan pada ikan asin adalah formalin, dimana zat kimia tersebut berbahaya bila dikonsumsi oleh manusia. Penelitian ini bertujuan untuk mengidentifikasi bahan pengawet formalin pada sampel ikan asin jambal roti, ikan asin kepala batu, ikan asin belah, ikan asin gembung, ikan asin gurami, ikan asin gabus, ikan asin malung, ikan asin gulamo, ikan asin nila, dan ikan asin tenggiri yang terdapat di Pasar tradisional kota Pekanbaru dan apakah ikan asin tersebut layak dikonsumsi. Uji kualitatif formalin dilakukan pada sampel ikan asin dengan menggunakan metode pereaksi Schiff dan tes kit. Hasil penelitian menunjukkan 10 dari 10sampel ikan asin yang diperiksa menggunakan pereaksi schiff positif mengandung formalin dan 7 dari 10 sampel ikan asin yang diperiksa menggunakan metode tes kit positif berformalin. Menurut Permenkes RI No. 722/Menkes/Per/IX/88 dan No. 1168/Menkes/Per/IX/1999 penggunaan formalin pada bahan tambahan pangan dilarang. Terdapat kandungan formalin pada ikan asin yang dijual di pasar tradisional kota Pekanbaru sehingga dinyatakan tidak aman untuk dikosumsi oleh masyarakat.  
POTENSI TOKSISITAS EKSTRAK ETANOL DAUN SALAM DAN KEJI BELING DENGAN METODE BSLT (Brine Shrimp Lethality Test) Alfin Surya; Andre Ajis Saputra; Hesti Marliza; Zaiyar
JURNAL KATALISATOR Vol. 8 No. 1 (2023): Jurnal Katalisator Volume 8 No. 1, April 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.596 KB) | DOI: 10.62769/katalisator.v8i1.2078

Abstract

A natural product that contains compounds that act as anticancer can be detected by conducting a preliminary test, namely the toxicity test. One of the methods in the Toxicity test is the BSLT test method as an initial step in the search for new anticancer compounds. The toxicity value of this method has been shown to correlate with cytotoxicity. anticancer compound. and can show a correlation to a specific anticancer. Bay plants contain saponins, triterpenoids, flavonoids, polyphenols, alkaloids, tannins, and essential oils consisting of sesquiterpenes, lactones, and phenols, which can be used as an alternative to prevent dyslipidemia, antioxidants, anti-inflammatories, antidiabetics, antidiarrheals and antihypertensives. Vile shard plant. contains various kinds of secondary metabolites and chemical substances such as potassium, sodium, silicic acid, calcium, saponins, alkaloids, polyphenols and flavonoids which act as antioxidants and can inhibit the growth and development of cancer cells. The purpose of this study was to compare the toxic effects of the ethanol extract of bay leaves and keji shard leaves on Artemia Salina Leach larvae. The type of research used was quantitative. The novelty of this research is to get the thick extract using sonification so that the time is more efficient compared to just doing maceration which takes longer. The results showed that the extracts of bay leaves and keji shard leaves are very toxic so they have the potential to be anticancer, as evidenced by the LC50 values of the bay leaf and koji shard leaf extracts which were 32.35 ppm and 194 ppm. From the LC50 value, bay leaves have higher toxicity than bitter shard leaves. The results obtained in this study can be used as an illustration for further research for the export of natural plant materials that have the potential as anticancer drugs.
UJI AKTIVITAS ANTIOKSIDAN EKSTRAK 2 PROPANOL DAUN SIRIH CINA (Peperomia pellucida) DENGAN METODE DPPH Alfin Surya; Alvira Rahma Fissilmi; Hesti Marliza; Zaiyar
JURNAL KATALISATOR Vol. 8 No. 2 (2023): Jurnal Katalisator Volume 8 No. 2, Oktober 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v8i2.2532

Abstract

Daun sirih cina secara empiris dapat digunakan sebagai obat sakit kepala, obat jerawat, dan obat nyeri perut pada beberapa penelitian juga di laporkan ekstrak etanol tanaman daun  sirih cina (Peperomia pellucida) diduga memiliki kandungan antioksidan yang baik bagi tubuh. Namun belum ada penelitian yang melaporkan bagaimana aktivitas antioksidan dari ekstrak 2 propanol daun sirih cina dimana dari tingkat kepolaran pelarut propanol lebih polar dibanding etanol dan methanol. Kebaharuan dalam penelitian ini adalah menggunakan 2 propanol sebagai pelarut untuk mendapatkan ekstrak daun sirih cina. Tujuan penelitian untuk mengetahui bagaimana aktivitas ekstrak 2 propanol daun sirih cina. Metode yang digunakan untuk mendapatkan nilai aktivitas antioksidan. Penelitian ini merupakan penelitian eksperimental laboratorium. Daun sirih cina dikeringkan dan dihaluskan kemudian dimaserasi dengan larutan 2 propanol. Selanjutnya dilakukan pengujian aktivitas antioksidan dengan metode DPPH (1,1-Dyphenyl-2-Pycrylhydrazyl) menggunakan alat microplate reader pada panjang gelombang 520 nm. Adapun hasil uji aktivitas antioksidan ekstrak 2 propanol daun sirih cina dengan nilai IC50 603,03 ppm yang menyatakan tingkatan aktivitas antioksidannya termasuk dalam kategori lemah. Dapat disimpulkan bahwa antioksidan ekstrak daun sirih cina menggunakan pelarut 2 propanol tidak lebih baik dalam menarik senyawa aktif
ANTIBACTERIAL ACTIVITY OF SEA GRAPES (Caulerpa racemosa) AGAINST Streptococcusmutans AND Shigella dysenteriae Sri Hainil; Hesti Marliza; Maria Yunivista; Rastria Meilanda
JURNAL KATALISATOR Vol. 8 No. 2 (2023): Jurnal Katalisator Volume 8 No. 2, Oktober 2023
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v8i2.2471

Abstract

The Riau Islands are known as an island group in Indonesia that is famous for its wealth of marine plants. Among the prominent marine flora, sea grape (Caulerpa racemosa) is a marine plant that produces secondary metabolites including alkaloids, saponins, phenolics, and flavonoids which have been proven to have antibacterial properties. Previous research showed that 70% ethanol extract from sea grapes exhibited strong antibacterial activity. However, there is no detailed information regarding the ability of the n-hexane and ethyl acetate fractions of sea grapes as antibacterials. This study aims to examine the antibacterial effects of n-hexane and ethyl acetate fractions from sea grapes against Streptococcus mutans and Shigella dysenteriae. The sea grape extraction process is carried out through the maceration method using 95% ethanol solvent, followed by the fractionation stage using the liquid-liquid extraction method. This fractionation process involves the use of non-polar (n-hexane) and semi-polar (ethyl acetate) solvents. The test method used was disk paper diffusion with varying fraction concentrations: 500 μg/disc, 400 μg/disk, 300 μg/disc, and 200 μg/disk. The positive control for this study used 30 μg/disc of tetracycline, because tetracycline is known as a broad-spectrum antibiotic. Meanwhile, the negative control used 10% DMSO. Findings from the research stated that the n-hexane fraction could not stop the growth of Streptococcus mutans bacteria, while the ethyl acetate fraction was able to inhibit the development of these bacteria. The average diameter at a concentration of 500 μg/disk was 9.2 mm, 400 μg/disk is 8 mm, 300 μg/disc is 7.7 mm, and 200 μg/disc is 6.5 mm. However, neither the ethyl acetate nor n-hexane fractions showed an inhibition zone against Shigella dysenteriae bacteria
ANTI-ANTIOXIDANT ACTIVITY LEAVES ANANASA COMOSUS IS MEDIUM USING THE DPPH METHOD alfin_surya; Fitri Harlina Sagala; Hesti Marliza; Zaiyar; Eliya Mursyida
JURNAL KATALISATOR Vol. 9 No. 2 (2024): Jurnal Katalisator Volume 9 No.2, Oktober 2024
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v9i2.3110

Abstract

One of Indonesia's best fruit plants, the pineapple (Ananas comosus), has a high antioxidant content. Ananas comosus, or pineapple leaves, are a component of the pineapple plant. They have slightly thick leaf sheaths with tiny brown thorns pointing upward at their margins. (Ananasa comosus). The purpose of this study is to investigate the pineapple (Ananas comosus) leaf's potential as a natural antioxidant source. The DPPH technique is being utilized in a descriptive quantitative research study to investigate the antioxidant capacity of pineapple leaves. Ananas comosus, or dried and crushed pineapple leaves, were macerated in methanol solvent until the sample was completely submerged. The IC50 value ascertained by the linear regression equation is the parameter employed in this technique. After the extract has evaporated from the solvent, its absorbance is measured at 520 nm using a microplate reader and the DPPH technique. Pineapple leaves show moderate antioxidant activity against DPPH with an IC50 value of 113.069 ppm, according to data from calculations and study. According to this study, the antioxidant capacity of pineapple leaves (Ananas comosus) is moderate.
ANTIOXIDANT ACTIVITY OF THE METHANOL EXTRACT OF TURKEY EGGPLANT (Solanum betaceum Cav.) INVITRO Surya, Alfin; Anggi Wulandari; Hesti Marliza; Zaiyar
JURNAL KATALISATOR Vol. 9 No. 1 (2024): Jurnal Katalisator Volume 9 No.1, April 2024
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62769/katalisator.v9i1.2871

Abstract

Duch Eggplant rich in phytochemicals, such as polyphenols, flavonoids, and carotenoids. Studies have demonstrated the powerful antioxidant activity of these compounds, which can shield body cells from free radical-induced oxidative damage.The aim of this study is to determine the IC50 value of the antioxidant activity present in the skin of the Holland turnip. This study also has implications for human health. Knowing the anti-oxidant skin activity in the inviter allows us to estimate the potential benefits of protecting the body from oxidative damage. 1.1-diphenyl-2-picrylhydrazil (DPPH) is the method employed. This method involves measuring the ability of the extract to neutralize the free radicals in DPPH. We will observe the color changes from purple to yellow and evaluate the free radical's capture capacity based on the degree of color change. The study's findings revealed an IC50 value of 41,019 ppm. The conclusion from the IC50 values obtained is that the methanol extract in the skin of the Dutch teranga has an antioxidant activity that is very strong to counter free radicals. 
Co-Authors Adella Adella Alfin Surya Alfin Surya Alfin Surya alfin_surya Alvira Rahma Fissilmi Amelia, Ayu Andre Ajis Saputra Anggi Wulandari Aprilya Sri Rachmayanti Apriyani Efendi Arie Vonikartika Arie Vonikartika Arini, Larasuci Cindy Octavia Delladari Mayefis Delladari Mayefis Delladari Mayefis Delladari Mayefis Delladari Mayefis Desy Maniarti Gusmali Diani Mega Sari Didi Yunaspi Dimas Prada Sumitra Dita Oktaviani Elfasyari, Trie Yuni Eliya Mursyida Fadillah, Ainun Faziyana Fenida, Rizka Fitri Harlina Sagala Fitri Nadira Fitri Ramadhani Ghiffari, Habibie Deswilyaz Gusmali, Desy Maniarti Haqqelni Nur Rosyidah Hasan, Nahrul Hayat, Nahrul Henny Rachdianti Tjahjono Suyarto Henny Rachdianti Tjahjono Suyarto Henny Rachdiati Maharani, Refina Maria Yunivista Mawardi Badar Mawardi Badar Mayefis, Delladari Meilanda, Rastria Nahrul Hayat Nanda Siska Nanda Siska Nurlia Oktavianty Nurlia Oktavianty Nurliyasman Nurliyasman Nurliyasman Nurliyasman Nurwijayanti Pratiwi Oktavia Pratiwi, Reri Qoirina Raihani Islamiati Rastria Meilanda Rastria Meilanda RENY HARYANI Reny Haryani Rizka Fenida Rury Trisa Utami Rury Trisa Utami Sammulia, Suci Fitriani Sarina Sembiring Milala Siahaan, Anggel Erggita Siska, Dedi Sri Hainil Sri Hainil Sri Hainil Sri Hainil Sri Hainil, Sri Sri Rahayu Melpa Sari Sri Rahayu Melpa Sari Suci Fitriani Sammulia Suci Fitriani Sammulia Suci Fitriani Sammulia Suhaera Suhaera Suhaera Suhaera Suhaera Suhaera T. Eltrikanawati Trinur Atika Saputri Utami, Rury Trisa Utamii, Rury Trisa Veranica Lestari Yelmiza Yoan De Nanda Herru Yufiradani, Yufiradani Yuliyana Yuliyana Yunisa Friscia Yusri Yunisa Friscia Yusri Zaiyar Zaiyar zaiyar Zaiyar, Zaiyar