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All Journal AQUATIC SCIENCE & MANAGEMENT JURNAL ILMIAH PLATAX Jurnal Ilmiah Tatengkorang Jurnal Mandala Pengabdian Masyarakat
Balansa, Walter
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Environmental Science Immunology & microbiology Public Health Social Sciences

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Journal : JURNAL ILMIAH PLATAX

 1 
A New and Practical Method for Measuring Sponge Spicules Rieuwpassa, Frets J.; Tomasoa, Aprelia M.; Palawe, Jaka F. P.; Rieuwpassa, Fredrik; Mege, Revolson Alexius; Balansa, Walter
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.47882

Abstract

Binocular light microscopy (BLM) is an excellent match for a scanning electron microscope (SEM) and a trinocular light microscope equipped with a micrometer (TLM). The practicality, user-friendliness, and short-time analysis of BLM make this method a good choice for spicule analysis. However, its effectiveness and accuracy are yet to be confirmed. This study aimed to validate the effectiveness of BLM by comparing its usefulness to both TLM and the gold standard methods. BLM was first subjected to measuring megascleres and microscleres of 2 sponges. Then, by using the If function built-in Excell and t-test in SPSS 16.0, the compatibility of BLM was evaluated against SEM by measuring the length of spicules from 4 Sangihe sponges and their counterpart species from different locations. Furthermore, the t-test analysis was used to validate the compatibility and effectiveness of our method to the TLM by measuring the spicules of four sponges. Both the F-function and the t-test analysis proved BLM was compatible with SEM with both measurements showing a perfect match for megascleres typed spicules of 4 compared sponges. This new technique also showed a perfect match with SEM (p = 0.367, t-test) and with TLM (p = 0.963, t-test). Keywords: Spicules, sponges, SEM, Wallacea, biomaterial, sponge taxonomy
Exploring Metabolites Of Green Algae Caulerpa Spp. To Discover Putative Inhibitors Of Dpp-4, A New Antidiabetic Target Protein: Molecular Docking Compounds from Marine Green Algae Balansa, Walter
Jurnal Ilmiah Platax Vol. 11 No. 2 (2023): ISSUE JULY-DECEMBER 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i2.48061

Abstract

Diabetes mellitus (DM) remains a serious global health threat, claiming a million lives every year and affecting nearly 9% of the adult population who suffer from this impaired insulin sensitivity disease. The Dipeptidyl peptidase-4 (DPP-4) enzyme has recently attracted attention because of its crucial role in insulin signaling, making this enzyme an interesting and emerging target for antidiabetic drug discovery. This study aimed to explore reported metabolites from marine algae of the genus Caulerpa as DPP-4 inhibitors through computational studies using CB-dock 2, Protein-Ligand Interaction Profiler, SwissAdme, and pkCMS. Molecular docking allowed the identification of 7 hit compounds with strong binding affinities (8.4 kcal to 9.3 kcal/mol) against DPP-4 target enzyme PDB ID: 3G4I. Four hits showed stronger binding affinity than two DPP-4 specific inhibitors and FDA-approved antidiabetic drugs, sitagliptin (8.4 kcal/mol) and linagliptin (9.0 kcal/mol). Following a molecular modification of the hit compounds using a bioisosterism-like approach and ADMET evaluation with pkCMS, three putative DPP-4 inhibitors were identified. They showed either stronger binding affinities or better ADMET profiles than sitagliptin and linagliptin, suggesting their promising potential as DPP-4 inhibitors. However, further optimized bioisoterism, in silico and in vivo studies, and clinical-based trials are required to confirm their antidiabetic activity. Keywords: Antidiabetic, Caulerpa racemosa, caulerpin, DPP-4
Antifungal Potential Of The Sponge Styllisa Flabelliformis Against The Pathogenic And Resistant Aspergillus Fungi Tomasoa, Aprelia Martina; Rieuwpassa, Frets Jonas; Azhari, Deidy; Ijong, Frans Gruber; Mege, Revolson Alexius; Tanod, Wendy Alexander; Balansa, Walter
Jurnal Ilmiah Platax Vol. 11 No. 1 (2023): ISSUE JANUARY-JUNE 2023
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v11i1.48603

Abstract

Together with bacterial and viral infection, fungal infection represents the world's top ten killer diseases, desperately requiring new antifungal drugs. This research aimed to evaluate the antifungal activity of the sponge Styllisa flabelliformis against the pathogenic fungi Aspergillus flavus and Aspergillus parasiticus by the standard agar diffusion technique. Three concentrations (1, 10, and 10 mg/mL) for the extract and 0.5 mg/mL for ketoconazole and fluconazole were prepared and evaluated in triplicate against the tested fungi. Whereas ketoconazole poorly inhibited A. parasiticus and fluconazole weakly inhibited A. flavus, the extract of S. flabelliformis exerted antifungal activity against A. parasiticus (6.8 ± 1.8; 8.3 ± 3.2; and 9.5 ± 2.1) mm and A. flavus (6.8 ± 1.1; 11.5 ± 1.4; and 14.3 ± 1.1) mm 1, 10 and 100 mg/mL respectively. PASS analysis showed jasplakinolide as a promising antifungal agent with potential activity (Pa) of 0.736. STITCH analysis further confirmed that jasplakinolide worked by inhibiting the expression of cytoskeleton genes that prevented fungi from synthesizing chitin and inhibiting the formation of the fungi’s cell walls and hyphae, different from the ergosterol synthesis inhibition in ketoconazole and fluconazole, implying the potential of jasplakinolide as an antifungal agent.
In-Silico Optimization of Macrolactin A from Sponge-Associated Bacteria and Its Derivatives as Eco-Friendly Antifoulants Balansa, Walter; Riyanti; Hinonaung, Jelita Siska Herlina
Jurnal Ilmiah PLATAX Vol. 13 No. 2 (2025): ISSUE JULY-DECEMBER 2025
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jip.v13i2.65278

Abstract

Marine biofouling causes significant economic and environmental damage creating an urgent demand for eco-friendly antifoulants. This in silico study aimed to evaluate the antifouling efficacy and ecotoxicological profile of macrolactin A (1), previously isolated from Indonesian sponge-associated Bacillus spp., alongside its computationally generated derivatives (1a-1f, 2a–2k) using Biotransformer 3.0. Molecular docking was utilized to assess binding affinities against key protein targets—the bacterial BAM complex, GSK-3 , and acetylcholinesterase (AChE)—and used EPI Suite™ to predict environmental safety. All tested compounds exhibited robust binding affinities (  kcal/mol) against all targets likely binding to allosteric sites. ANOVA revealed significant differences ( ) in binding strength, with derivatives displaying a distinct preference for GSK-3  over AChE and BAM ( ). However, the broad-spectrum affinity across all three targets supports a potential multi-mechanism mode of action. Crucially, most derivatives showed low toxicity and bioaccumulation potential compared to commercial antifoulants such as Irgarol 1501, SeaNine 211, and Selektope®. Notably, analogues 1a-1c and 2e were predicted to be readily biodegradable. This study identified 1a–1c and 2e as leading candidates for eco-friendly antifoulants and provides a strong basis for future experimental development of novel, sustainable marine coating candidates. Keywords: ecofriendly antifouling; in silico; macrolactin; molecular docking; sponge-associated bacteria
Co-Authors Aprelia Martina Tomasoa, Aprelia Martina Arie Wibowo Azhari, Deidy Barlian, Anggraini Devi Nandita Choesin, Devi Nandita Dzaky, Muhammad Luqman Frans Gruber Ijong Frets Jonas Rieuwpassa Gansalangi, Ferdinand Gansalangi, Ferdinang Hinonaung, Jelita Siska Herlina Langi, Edwin O. Makawekes, Steward Imanuel Melupite, Billy Mozes, Getruida Nita Nita Yuanita, Nita Nursatya, Safira Meidina Palawe, Jaka F. P. Revolson Mege Rieuwpassa, Fredrik Rieuwpassa, Frets Rieuwpassa, Frets J. Riyanti Sidauruk, Gaberaja Samuel Roosevelt Tomasoa, Aprelia Tomasoa, Aprelia M. Wendy Alexander Tanod Wibowo, Indra Wuaten, Julius Frans Yana Sambeka