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The Role of Intrinsic Pathway Apoptosis via Caspase-9 in Atherogenesis Due To Atherogenic Diet in Sprague Dawley Rats Yanuartono Yanuartono; Hastari Wuryastuti; R Wasito; Sri Raharjo
Indonesian Food and Nutrition Progress Vol 12, No 1 (2005)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.32

Abstract

Thirty male rats, strain Sprague Dawley were used as experimental animal to study the role of intrinsic (mitochondrial) pathway apoptosis in atherogenesis due to high fat and high cholesterol diet. The rats were randomly alloted into three group (I, II, III) of 15 each. Group I as control was fed normal diet, group II was fed diet containing high fat diet, and group III was fed containing high fat and high cholesterol diet (atherogenic diet). After 6 and 12 weeks on experimental diet, 15 rats were selected randomly (5 rats of each group). All animal were then killed and the aorta were taken out for caspase-9 immunohistochemical analysis. Based on the present study result it can be concluded that high fat diet and high cholesterol diet could induced apoptosis through caspase-9.
The Role of Extrinsic Pathway (Death Receptor Pathway) Apoptosis through Caspase-8 in Atherogenesis due to High Fat and High Cholesterol Diet Yanuartono Yanuartono; Hastari Wuryastuti; R Wasito; Sri Raharjo
Indonesian Food and Nutrition Progress Vol 12, No 1 (2005)
Publisher : Indonesian Association of Food Technologists

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jifnp.33

Abstract

Thirty male rats, strain Sprague Dawley were used as experimental animal to study the role of death receptor pathway apoptosis in atherogenesis due to high fat and high cholesterol diet. The rats were randomly alloted into three group (I, II, III) of 10 each. Group I as control was fed normal diet, group II was fed diet containing high fat diet, and group III was fed containing high fat and high cholesterol diet (atherogenic diet). After 6 and 12 weeks on experimental diet, 15 rats were selected randomly (5 rats of each group). All animal were then killed and the aorta were taken out for caspase-8 immunohistochemical analysis. Based on the present study result it can be concluded that high cholesterol and/or high fat diet induced apoptosis through death receptor pathway via caspase-8.
Strongyloidiasis in Bornean Orangutan: A Case Report Suandhika, Putu; Susanti, Agatha Ria; Sriningsih, Agnes Pratamiutami; Hina, Carlo Yunior Ray; Yanuartono, Yanuartono; Indarjulianto, Soedarmanto; Priyowidodo, Dwi
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.297-302

Abstract

This study reported the diagnosis and treatment of Strongyloidiasis in two Bornean orangutans (Pongo pygmaeus); adult male aged 23 years, weighing 100 kg and an infant female orangutan, 4 years old, weighing 13,6 kg. Samples were physically and laboratory examined for faeces using the Mini-FLOTAC method. Therapy was performed on the adult orangutan using albendazole at a dose of 400 mg individual twice a day for 4 days, orally. The infant orangutan was administered albendazole at a dose of 10 mg/kg BW once a day for 3 days orally. From several physical examinations, both orangutans looked active, body temperature and oral mucosa color were normal, green feces with solid consistency in the adult orangutan and brown with solid consistency in the infant orangutan. Microscopic faeces examination of the adult orangutan showed Strongyloides sp. as many as 1005 eggs per gram (EPG) of feces, and the infant orangutan as many as 2490 larvae per gram of feces. Both orangutans were diagnosed with severe Strongyloidiasis based on those examinations. Three days after starting treatment, the adult orangutan's EPG decreased to 890, and a week later, no Strongyloides sp. eggs or larvae were discovered. A week following treatment, no Strongyloides sp. was discovered in an infant orangutan. In conclusion, albendazole was an effective treatment for severe Strongyloidiasis in adult and infant orangutans. Strongyloidiasis can be a threat to both infant and adult orangutans without distinct clinical symptoms.
Case report Diagnosis dan Penanganan Feline Panleukopenia Nareswari, Anggitya; Indarjulianto, Soedarmanto; Yanuartono, Yanuartono; Purnamaningsih, Hary; Widiyono, Irkham
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100434

Abstract

Feline panleukopenia (FPL) adalah penyakitinfeksi Feline panleukopenia virus yang menyebabkan mortalitas tinggi pada kucing. Penelitianini bertujuan melaporkan diagnosis dan penanganan kasus FPL. Studi kasus ini menggunakan seekor kucing persia mix, betina, berumur empat bulan yang diperiksa secara klinis, uji FPV-Ag dan hematologi. Hasil anamnesis dan pemeriksaan klinis didapatkan bahwa kucing lesu, tidak mau makan, muntah, dan mengalami diare berwarna coklat, suhu tubuh 40,3⁰C, dehidrasi, dan selaput lendir anemis. Hasil pemeriksaan hematologi menunjukkan kucing mengalami leukopenia, anemia dan FPV-Ag test positif, sehingga kucing didiagnosis menderita FPL. Kucing diberi terapi infus Ringer Lactate 30ml/kgBB/ hari IV, enrofloxacin 5 mg/ kgBB/hari SID SC dan Amoxicilin 10 mg/kgBB dua hari sekali IM, hematopoietin  0,1 ml/kgBB BID IV, vitamin-Asam amino 0.1 ml/ kgBB BID IM. Kucing dirawat secara intensif dan mati pada hari ketiga. Disimpulkan bahwa kucing pada kasus ini didiagnosis FPL berdasar gejala klinis, leukopenia dan reaksi positip tes FPV-Ag. Kucing penderita FPL tersebut telah diberi terapi cairan, antibiotik, dan vitamin, tetapi tidak berhasil disembuhkan.
Zinc deficiency in ruminants and its management: A brief review Yanuartono, Yanuartono; Indarjulianto, Soedarmanto; Paryuni, Alsi Dara
Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science) Vol. 14 No. 3 (2024): Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Ve
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/jipvet.v14i3.408

Abstract

Minerals are organic substances found in nature and living things. In ruminants, minerals are one of the crucial components of the body. One of the mineral elements that is very important for the body is Zinc (Zn). The prominent role of Zn in ruminants is the synthesis and degradation of carbohydrates, fats, proteins, and nucleic acids. Ruminants can experience Zn deficiency by showing clinical symptoms such as dermatitis, anorexia and parakeratosis, stunted growth, and nail damage. In contrast, ruminants can also experience Zn poisoning with various clinical symptoms such as general weakness, diarrhea, hemolytic anemia, and decreased body weight. Current Zn deficiency can be overcome by adding commercial supplement products containing Zn. This paper aims to add brief insights into the benefits and treatment of Zn deficiency in ruminants.
Limbah Industri Perunggasan dan Manfaatnya sebagai Sumber Protein Ruminansia: Ulasan Singkat Yanuartono, Yanuartono; Indarjulianto, Soedarmanto; Paryuni, Alsi Dara
Jurnal Peternakan Vol 21, No 2 (2024): September 2024
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v21i2.26476

Abstract

ABSTRAK. Peningkatan produksi daging global selama beberapa tahun terakhir terutama didorong oleh produksi daging unggas yang lebih efisien karena usaha perunggasan terus berkembang lebih maju di banyak bagian dunia. Metode peningkatan pemanfaatan penggunaan pakan asal limbah industri perunggasan telah diusulkan sebagai solusi untuk meningkatkan efisiensi pemanfaatan limbah, mengurangi kompetisi pemanfaatan pangan maupun pakan dan meningkatkan system daur ulang yang efisien. Limbah industri perunggasan yang dapat dimanfaatkan sebagai sumber protein ruminansia antara lain adalah tepung bulu, limbah penetasan ayam, kaki ayam, tepung jeroan, tepung darah, tepung tulang, manure dan litter unggas. Limbah industri peternakan unggas tersebut dapat dimanfaatkan kembali ke dalam rantai makanan, setelah melalui berbagai macam perlakuan. Penggunaan limbah unggas sebagai sumber protein ternak ruminansia telah lama diteliti dan diterapkan di lapangan. Tulisan ini bertujuan memberikan ulasan singkat pemanfaatan limbah industry perunggasan sebagai sumber protein alternatif untuk ruminansia.Kata kunci: Unggas; limbah; protein; ruminansiaPoultry industry waste and its benefits as a source of protein for ruminants: a brief reviewABSTRACT. The increase in global meat production over recent years has been driven primarily by more efficient poultry meat production as poultry businesses continue to advance in many parts of the world. Methods for increasing the use of feed from poultry industry waste have been proposed to increase waste utilization efficiency, reduce competition for food and feed use, and improve an efficient recycling system. Poultry industry waste that can be used as a source of ruminant protein includes feather meal, chicken hatching waste, chicken feet, offal meal, blood meal, bone meal, manure, and poultry litter. After various treatments, poultry farming industrial waste can be reused into the food chain. The use of poultry waste as a ruminant protein source has long been researched and applied in the field. This paper will briefly review the use of poultry industry waste as an alternative protein source for ruminants.
Manfaat Feed Additives pada Ruminansia: Ulasan Singkat Yanuartono, Yanuartono; Soedarmanto, Indarjulianto; Paryuni, Alsi Dara
Jurnal Peternakan Vol 22, No 1 (2025): Februari 2025
Publisher : State Islamic University of Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/jupet.v22i1.30061

Abstract

ABSTRAK. Seiring dengan meningkatnya permintaan produk hewani, maka kebutuhan pakan ruminansia juga semakin meningkat. Pemenuhan kebutuhan pakan bertujuan untuk menghasilkan produk hewan yang lebih baik, memanfaatkan sumber daya pakan yang tersedia secara efisien, dan memaksimalkan keuntungan peternak. Salah satu upaya untuk memaksimalkan produk hewani adalah dengan menambahkan feed additives pada pakan. Bahan tambahan pakan merupakan bahan pakan non-nutrisi yang ditambahkan ke dalam campuran pakan untuk tujuan tertentu dan saat ini mempunyai peranan penting dalam berbagai bidang produksi ternak ruminansia. Berbagai bahan tambahan pakan telah digunakan di bidang peternakan untuk meningkatkan produksi ternak, meningkatkan efisiensi sumber daya pakan yang tersedia, dan memaksimalkan kinerja ternak ruminansia. Artikel singkat ini mengulas manfaat feed additive bagi ternak ruminansia.Kata kunci: Feed additives, produksi ternak, non-nutrisi, ruminansiaBenefits of Feed Additives for Ruminants: A Brief ReviewABSTRACT. As demand for animal products increases, the need for ruminant feed also increases. Fulfilling feed needs aims at better animal production, efficiently utilizing available feed resources, and maximizing farmers' profits. One effort to maximize animal products is by adding feed additives to animal feed. Feed additives are non-nutrient feed ingredients added to feed mixtures for specific purposes and currently have an essential role in various fields of ruminant livestock production. Various feed additives have been used in the livestock sector to improve livestock production, increase the efficiency of available feed resources, and maximize the performance of ruminant livestock. This short article reviews the benefits of feed additives for ruminant livestock.
GAMBARAN VAKSINASI FELINE PANLEUKOPENIA PADA KUCING DI KLINIK DEPARTEMEN ILMU PENYAKIT DALAM FKH -UGM DESCRIPTION OF FELINE PANLEUKOPENIA VACCINATION IN CAT AT THE CLINIC OF THE DEPARTMENT OF INTERNAL MEDICINE FKH UGM Purnamaningsih, Hary; Indarjulianto, Soedarmanto; Widiyono, Irkham; Hartati, Sri; Yanuartono, Yanuartono; Raharjo, Slamet; Nururrozi, Alfarisa
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.70828

Abstract

Abstract              Feline Panleukopenia is a disease caused by a DNA type virus, family Parvoviridae with high morbidity and mortality in the Felidae family group (Kruse et al., 2010; Hartmann, 2017). The study also shows that the prevalence rate of the disease has increased for 3 years since 2015. The report of Kusumawardani et al (2015) shows that 97 cases of cats were diagnosed with FPV. The vaccination program has not been implemented optimally by the cat-keeping community. The incidence of the disease is still common and cases can increase in certain seasons. Several cases handled at the Clinic of the Department of Internal Medicine, FKH-UGM ended in death. There has been no report on the implementation of vaccination in animal health care places. It is necessary to study the vaccination program for cats carried out by the cat-keeping community as an effort to prevent Feline Panleukopenia disease so that it can provide information about FPV vaccination as a basis for optimizing the implementation of FPV vaccination in cats. The study used ambulatory card data from cat examinations as clinical patients of the Department of Internal Medicine, FKH UGM in 2019.  Recap data collected in the form of ambulances for cat patients who requested vaccination. Patient data provided with vaccination services are grouped based on cat identity, age, sex, cat breed/race and maintenance system. The data obtained were tabulated and analyzed descriptively. Based on the results of the recap of ambulatory data for cat patients in 2019 who were vaccinated against feline panleukopenia, 236 individuals were obtained. The age of vaccinated cats at the age of <6 months were 128 animals (54%) and 108 cats (46%). there were 108 cats (46%) male cats and 128 (54%) female cats vaccinated against Feline panleukopenia. Types/breeds of cats, which were vaccinated against feline panleukopenia in purebred cats (Persi, Angora and others) as many as 128 (54%), 35 domestic cats (15%) and 73 mixed-breed cats (31%) ). Based on the study of this research data, it can be concluded that the feline panleukopenia virus vaccination program in male domestic cats is still low compared to purebred cats Keywords : Feline Panleukopenia Virus, cat, vaccination
Sexing Burung Lovebird Fischer (Agapornis fischeri) Berdasarkan Perkembangan Berat Badan Fatona, Desnita Rizka; Permana, Rief Ghulam Satria; Akrom, Afif Muhammad; Yanuartono, Yanuartono; Indarjulianto, Soedarmanto
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.96593

Abstract

Lovebird fischer (Agapornis fischeri) merupakan burung yang banyak diminati karena memiliki warna bulu yang cantik serta suara atau kicauan yang merdu dan lantang. Jenis kelamin burung lovebird biasanya baru dapat dilakukan ketika burung sudah dewasa, tetapi pada umur tersebut suara sulit untuk dibentuk. Perkembangan berat badan LB sampai umur 60 hari merupakan salah satu faktor yang dipakai untuk sexing. Penelitian ini bertujuan membandingkan pekembangan berat badan LB muda sebagai dasar sexing. Penelitian ini menggunakan 12 ekor burung lovebird umur 30 hari. Burung dipastikan jenis kelaminnya berdasarkan sexing secara genotip dengan metode polymerase chain reaction (PCR) untuk amplifikasi gen CHD1 menggunakan pasangan primer CHD1F/CHD1R dengan sampel bulu. Semua burung ditimbang berat badan pada hari ke-30, 45 dan 60. Hasil pengukuran berat badan antara jantan dan betina dibandingkan menggunakan uji t-Test. Hasil penelitian didapatkan bahwa berat badan LB betina umur 30, 45 dan 60 hari berturut-turut adalah 19,81±4,50 gram, 32,29±6,30 gram dan 42,72±3,29 gram, sedangkan LB jantan 19,11±4,72 gram, 31,32±5,82 gram dan 41,96±3,19 gram, namun demikian tidak ada perbedaan nyata antara betina dan jantan (p<0.05). Disimpulkan bahwa berat badan LB umur 30, 45 dan 60 hari tidak berbeda nyata, sehingga tidak dapat dipakai sebagai dasar sexing burung LB.