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All Journal HAYATI Journal of Biosciences Biotika: Jurnal Ilmiah Biologi AL KAUNIYAH Jurnal Sain Peternakan Indonesia Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Surya Octagon Interdisciplinary Journal of Science and Technology Floribunda ASEAN Journal of Community Engagement Current Research on Biosciences and Biotechnology Makara Journal of Science
Dwiranti, Astari
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Indonesia, Depok, 16424, Indonesia, (021) 7270163.
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Assessment of potential cellular toxicity of methylglyoxal on primary human epidermal keratinocytes Prakoso, Nurul Muhammad; Sundari, Ayu Mulia; Fadhilah; Abinawanto; Dwiranti, Astari; Bowolaksono, Anom
Current Research on Biosciences and Biotechnology Vol. 6 No. 2 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.6.2/JYICDMXO

Abstract

Skin is the outermost layer of the human body, which is important to prevent damage by infection, ultraviolet radiation, and toxic materials. The impermeable barrier of the skin has to be maintained via skin regeneration to preserve its essential function. However, skin regeneration declines due to decreased cell proliferation during skin aging. In this study, we investigated the role of methylglyoxal (MGO) as a precursor of advanced glycation end-products (AGE), which have been reported as an emerging threat of early aging in the skin. Previous studies reported a reduction in survival of the HaCaT cell line exposed to 500 µM MGO. However, studies on primary keratinocytes are still limited. We evaluated the viability and proliferation of primary human epidermal keratinocytes (HEKa) under the exposure of MGO. We also employed H2DCFDA assay to quantify the generation of intracellular ROS due to MGO. Our study revealed a biphasic response in which lower doses of MGO (25 and 50 µM) increased viability, while the cellular toxicity began at higher concentrations of 100, 200, and 300 µM. The proliferation assay also suggests an overall reduction of primary epidermal keratinocytes exposed to 100 and 200 µM MGO after 24 and 48 hours of culture. Increasing ROS concentrations were also observed in cells treated with 100 and 200 µM MGO. Our data suggested that a lower concentration of MGO was required to induce cellular toxicity in primary human epidermal keratinocytes compared to the HaCaT cell line reported by another study, indicating different sensitivity to MGO.
Caffeine as a modulator of redox balance and migration in MDA-MB-231 triple-negative breast cancer cells Amira, Qanita Hana; Fadilah; Bowolaksono, Anom; Dwiranti, Astari
Current Research on Biosciences and Biotechnology Vol. 6 No. 2 (2025)
Publisher : Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/crbb.2025.6.2/54S7U9DO

Abstract

Caffeine, a widely consumed stimulant, has demonstrated significant effects on cancer cell behavior, particularly in triple-negative breast cancer (TNBC) cells. This study investigates the impact of caffeine on MDA-MB-231 cells, focusing on cell morphology, viability, antioxidant gene expression, and cell migration. Caffeine at concentrations of 10 mM and above induced notable morphological changes, including cell rounding, detachment, and decreased cell density, indicative of cytotoxic effects and cellular stress responses such as apoptosis. Viability assays revealed a dose-dependent reduction in cell survival, with a substantial decline in total cell count at higher caffeine concentrations. The observed decrease in cell viability is associated with the downregulation of antioxidant genes SOD2 and GLO1, suggesting disrupted redox balance and impaired detoxification systems. A significant positive correlation was noted between SOD2 and GLO1 expression levels, indicating their interdependence in antioxidant defense mechanisms. Additionally, caffeine treatment impaired cell migration, as the wound healing assay shows, with higher concentrations significantly hindering wound closure. This effect on migration, relevant for metastasis, aligns with findings from previous studies on caffeine’s influence on cancer cell motility. The results suggest that 10 mM caffeine may serve as an optimal concentration for inducing cellular stress without immediate, widespread cell death, positioning it as a promising candidate for further therapeutic exploration.
Cloning and Expression of SCAMP3 in Escherichia coli BL21(DE3) with In Silico Sequence-Based Cancer Epitopes Prediction Rajagukguk, Selly Setiati; Pambudi, Sabar; Dwiranti, Astari; Utomo, Doddy Irawan Setyo; Bowolaksono, Anom
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

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Abstract

Secretory carrier membrane protein 3 (SCAMP3) is a crucial membrane protein involved in intracellular vesicle traffick-ing and exocytosis. The SCAMP3 expression has been observed in diverse cancer types, such as melanoma, glioma, hepatocellular and breast cancer. Increased SCAMP3 expression has been reported in certain cancer cells relative to that in normal cells, suggesting the potential role of SCAMP3 in cancer development or progression. In this study, we successfully cloned and expressed SCAMP3 in Escherichia coli strain BL21(DE3). SCAMP3 was amplified and insert-ed directionally into the prokaryotic expression vector pET21d(+). The transformation of recombinant plasmid into E. coli BL21(DE3) cells were performed for the protein expression. SDS–PAGE and Western blotting were performed to detect the expression product induced by IPTG, which confirmed the presence of a recombinant pET21d(+)-SCAMP3 at 38-kDa protein weight. Bioinformatics analyses helped discover several possible epitopes distributed throughout the SCAMP3 protein sequence. These findings together serve as a basis for future biochemical and functional studies on this important membrane protein alongside immunotherapy research related to SCAMP3 as a cancer biomarker.
A Multidimensional Welfare Status of Leprosy Patients Living in a Suburban Area Irawati, Yunia; Menaldi, Sri Linuwih SW; Harini, Melinda; Wahyuni, Luh Karunia; Alwin, Wanarani; Dwiranti, Astari; Menaldi, Adhityawarman; Luzanil, Sonny Tirta; Matsurah, Qaishum; ., Dadun; Kurniawan, Rico; Ruwaida, Ida; Wicaksono, Gunawan; Sahid, Muhammad Hidayat; Rahmayanti, Febrina; Priscilia, Florentina; Fitriana, Anis; Rahayu, Tri; Susiyanti, Made; Andayani, Gitalisa; Bani, Anna Puspitasari; Daniel, Hisar; Lestari, Yeni Dwi
ASEAN Journal of Community Engagement Vol. 5, No. 1
Publisher : UI Scholars Hub

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Abstract

Leprosy has a high incidence of physical complications that will impact directly the physiological, economic, and social status of patients. Indonesia has a high burden of the disease, and several leprosy settlements are still spread in the country. Neglasari Village, Sitanala, Tangerang serves as one of the places of residence of people who have had leprosy. A health service initiative known as Identifikasi Tanda-Tanda Mata, Ekstremitas, dan Kulit pada Kusta (KATAMATAKU) was launched as a collaboration of health services among multi-departments (ophthalmology, dermatovenereology, and medical rehabilitation). Sitanala has a relatively high incidence of people who have had leprosy with disabilities of the hand, foot, and eye. As a continuation of this health service and combined with the efforts to improve the welfare of leprosy patients, in November 2019, a multidisciplinary program titled KATAMATAKU Universitas Indonesia, was conducted. This program aimed to determine the demographic data regarding the health, psychological, social, and economic status of the leprosy population at Sitanala The project consisted of a collaborative anti-stigma program by the Faculty of Psychology, Public Health, Social and Political Sciences, Cultural Sciences, Administrative Sciences, and Vocational Educational Program; thematic health program, which supports the improvement of physical abilities and empowerment of former leprosy patients, by the Faculty of Medicine, Dentistry, Nursing, and Pharmacy; thematic economics program, which aims to increase the economic capacity of the leprosy community, by the Faculty of Economics and Business, Mathematics and Natural Sciences, and Engineering. This program enabled the construction of a multidimensional management model, in which every aspect plays important roles to improve the patients’ quality of life.
Foreword from Handling Editor - 7th Edition Dwiranti, Astari
ASEAN Journal of Community Engagement Vol. 4, No. 1
Publisher : UI Scholars Hub

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Abstract

We are delighted to present the 7th edition of AJCE, published in July 2020. We have selected twelve of the best articles for this edition, consisted of nine Research Articles, two Review Article, and one Case-Based Articles. These articles presented the valuable and interesting information with the broad ranges covering three sub-themes, i.e. education (young adult literature, cross-border development, self protection, developing community engagement with Social Life Cycle Assessment, Malay minorities), Economy (creative economy clinic, dairy cattle production), and Health (child health nutrition program, health education projects, empowering women in Covid-19, knowledge regarding Biliary Atresia). We want to express our thanks to the reviewers who gave inputs and contributions to improve the quality of the articles. Our greatest thanks are also due to Azhar Firdaus for helping us with the publication process and to the Directorate of Administration, Data, and Management of Research Product and Innovation (DADPPRI UI) for providing a journal development grant and proofread assistance through Enago. Finally, we hope that readers enjoy the articles that we present in this edition.
Foreword from Handling Editor – 8th Edition Dwiranti, Astari
ASEAN Journal of Community Engagement Vol. 4, No. 2
Publisher : UI Scholars Hub

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Abstract

We are delighted to present the 8th edition of AJCE, published in December 2020. We have selected eleven of the best articles for this edition, consisted of six Research Articles, one Review Article, and four Case-Based Articles. These articles presented the valuable and interesting information with the broad ranges covering three sub-themes, i.e. education (Community engagement in COVID-19 responses, intellectual property rights, and technique to decrease depression level), Economy (participatory budget during the Covid-19 pandemic, and Empowering Botswana’s rural communities), and Health (Development-induced displacement on the Tribal community, Combating COVID-19 infodemic, Village fund accountability and gendering, stunting early detection, and Training program to support posbindu cadre knowledge). We want to express our thanks to the reviewers who gave inputs and contributions to improve the quality of the articles. Our greatest thanks are also due to Azhar Firdaus for helping us with the publication process and to the Directorate of Administration, Data, and Research Product and Innovation Management Universitas Indonesia (DADPPRI UI) for providing a journal development grant, and proofread assistance through ENAGO. Finally, we hope that readers enjoy the articles that we present in this edition.
Co-Authors ., Dadun Abinawanto Abinawanto Abinawanto Adra Amalia Nur Ahlina Alwin, Wanarani Amira, Qanita Hana Andayani, Gitalisa Andi Salamah Anna Widyastuti ANOM BOWOLAKSONO Arief Boediono Atria, Mega Bani, Anna Puspitasari Daniel, Hisar Deti Purwanti Fadhilah Fadilah Febrina Rahmayanti Fitriana, Anis Funahashi, Hiroaki Harini, Melinda Ida Ruwaida, Ida Irawati, Yunia Iriani, Nur Annisa Isnaenisa Rachma Khayrani, Apriliana Cahya Kitty, Yelina Kurniawan, Rico Lischer, Kenny Luh Karunia Wahyuni, Luh Karunia Luzanil, Sonny Tirta Made Susiyanti Matsurah, Qaishum Menaldi, Adhityawarman Menaldi, Sri Linuwih SW Mouleidi Dwi Putri Nadhifa Tazkia Ramadhani Niarsi Merry Hemelda Niarsi Merry Hemelda Niarsi Merry Hemelda Nidaan Khafian Pambudi, Sabar Prakoso, Nurul Muhammad Priscilia, Florentina Putrika, Afiatry Rajagukguk, Selly Setiati Ratna Yuniati Sahid, Muhammad Hidayat SAHLAN, MUHAMAD Sarah Tsabituddinillah Sari, Elvita Shela Kartika Wijaya Siska Apriliani Sri Rahayu, Amy Yayuk Sundari, Ayu Mulia Tri Rahayu Utomo, Doddy Irawan Setyo Wicaksono, Gunawan Windri Handayani Windri Handayani Windri Handayani Wulandari, Tri Aprilliana Yeni Dwi Lestari