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Khusuma, Ari
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Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Utilization Of Kuku Pancar (Lawsonia Inermis Lin) Leaves as a Substitute For Eosin Dye in The Examination of Soil Transmitted Helminth (STH) Eggs Yusari, Lala Deswinta; Srigede, Lalu; Manu, Thomas Tandi; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.356

Abstract

Nail surfacing leaves (Lawsonia inermis lin) are natural materials commonly used as dyes, because of the content of chemical compounds in them such as lawsone, flavonoids, tannins and anthocyanins, therefore nail surfacing leaves can be used as an alternative dye to replace 2% Eosin in STH worm egg examination, because it has the same properties as 2% Eosin and can produce an orange yellow color after the extraction process. The research objective is to determine the results of nail surfacing leaves (Lawsonia inermis lin) can be used as an alternative substance to replace Eosin in the examination of STH (soil transmitted helminth) worm eggs. The methods of this research is using is experimental, where this study looks at the clarity of soil transmitted helminth (STH) worm eggs on preparations, using coloring from the results of soaking the leaves of the nail surf as a substitute for Eosin. The results of the research is that the coloring results obtained in each variation of immersion time are good. The conclusion of this research is that the staining of worm eggs using the results of immersion of nail surfacing leaves for 12 hours, 24 hours, 36 hours, and 48 hours can be used as an alternative dye in the examination of STH worm eggs.
Analisis Konsentrasi Letal Dan Waktu Letal Isolat Jamur Aspergillus Niger Terhadap Nyamuk Aedes Aegypti Kadek Sinta Cahyani, Ni Diah; Wiadnya, Ida Bagus Rai; Khusuma, Ari; Getas, I Wayan
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.264

Abstract

Latar Belakang: Nyamuk Aedes aegypti merupakan penyebab utama penyakit DBD, kasus DBD di dunia pada tahun 2015 sebanyak 3,2 juta kasus. Di Indonesia pada tahun 2018 terdapat 65.602 kasus. Upaya pengendalian terhadap vektor DBD telah banyak dilakukan baik dengan menggunakan insektisida kimia dan alami salah satunya dengan jamur Aspergillus niger sebagai fungi entomopatogen. Tujuan Penelitian: Mengetahui konsentrasi letal dan waktu letal dari isolat jamur Aspergillus niger terhadap nyamuk Aedes aegypti.  Metode Penelitian: Penelitian ini merupakan penelitian true eksperimental dengan rancangan acak lengkap. Perlakuan dengan 3 konsentrasi isolat jamur Aspergillus niger yaitu 10-5, 10-6, 10-7 spora/ml dengan 6 replikasi. Penelitian ini menggunakan obat nyamuk semprot sebagai kontrol positif. Data diperoleh dianalisa secara statistik menggunakan analisis probit. Hasil Penelitian: Konsentrasi isolat jamur Aspergillus niger yang efektif terhadap nyamuk Aedes aegypti adalah konsentrasi 10-7. Hasil analisis probit uji patogenitas isolat jamur Aspergillus niger terhadap nyamuk Aedes aegypti dengan tingkat kepercayaan 95% didapatkan LC50, LC70, LC90 adalah 6,1 x 107 spora/ml, 8,5 x 107 spora/ml, 1,1 x 108 spora/ml dan diketahui LT50, LT70, LT90 berturut-turut adalah 1,919 jam, 1,510 jam, 1,184 jam. Kesimpulan: Konsentrasi isolat jamur Aspergillus niger secara signifikan memberikan pengaruh terhadap kematian nyamuk Aedes aegypti.
Analisis Jumlah dan Jenis Bakteri Coccus Gram Positif pada Sisa Sampel Darah di Spuit Arfi, Sofiyan Dwi; Kristinawati, Erna; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 2 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i2.270

Abstract

.Background: One of the problems in the field of health services that can increase the mortality rate is nosocomial infection. Waste containing pathogenic bacteria can serve as a medium for the spread of disease for health facility workers, sufferers and the public. One of the medical devices that have a high risk factor as an intermediary for infection is a syringe. Bacterial contamination of the syringe after use cannot be ignored. Therefore, further research is needed on waste in health facilities to determine infectious diseases caused by residual blood samples in the syringe. Research Objectives: To determine the presence of gram-positive coccus bacteria in the remaining blood sample in the syringe. Methods: This study used a descriptive observational method with a cross sectional approach to time. The sample size in this study was 14 samples with a sampling technique that is total sampling. Results: It was found that Staphylococcus aureus and Staphylococcus cohnii were found each of the 14 samples. Conclusion: There are gram-positive coccus bacteria, namely Staphylococcus cohnii and Staphylococcus aureus. 
Efektivitas Penggunaan Cuka Apel (Apple Cider Vinegar) Terhadap Daya Hambat Pertumbuhan Staphylococcus Aureus Yang Diisolasi Dari Ulkus Diabetes Mellitus Novianty, Alfiah; Agrijanti, Agrijanti; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.200

Abstract

Diabetic ulcers are a chronic complication of Diabetes Mellitus in the form of open sores on the surface of the skin. Staphylococcus aureus bacteria is one of the bacteria that causes infection in wounds. Staphylococcus aureus bacteria will flourish in areas that have infections. An increasingly severe infection will cause gangrene. As it is known that Apple vinegar contains chemicals that are as antibacterial. Therefore apple vinegar can be used as a treatment in inhibiting the growth of Staphylococcus aureus bacteria. This study used a quasi-experimental design with 6 treatments, namely Tetracycline Antibiotics as a negative control of growth, sterile aquadest as a positive control of growth and apple vinegar with concentrations of 100%, 50%, 25%, and 12.5%. Then performed a statistical test using Kruskal Wallis with a confidence level of 95% or α = 0.05. Result: Sample Diabetic Foot Ulcer positively contained Staphylococcus aureus. Test the sensitivity that is done by using vinegar apple effective to inhibit the growth of Staphylococcus aureus at concentrations of 50% with a power resistor of 21.75 mm and a concentration of 100% by 24 mm. Conclusion: Apple Cider Vinegar effective to inhibit the growth of Staphylococcus aureus was isolated from Ulcer Foot Diabetic.
Pengaruh Pencucian Darah Domba dengan NaCl 0,85% Terhadap Pertumbuhan Bakteri Neisseria gonorrhoeae Pada Media CAP Agrijanti, Agrijanti; Intan Suryawatie Yomo, Meidiana; Khusuma, Ari; Kusuma Dewi, Lale Budi
Jurnal Analis Medika Biosains (JAMBS) Vol 9, No 1 (2022): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v9i1.259

Abstract

Neisseria gonorrhoeae is a fastidious organism. The growth medium for the bacterial culture of Neisseria gonorrhoeae is a Chocolate Agar Plate (CAP) medium enriched with additional nutrients such as sheep blood, vancomisin, nystatin, and isovitaleX. in general, the making of  CAP  is not washed (defibrinated) the sheep blood, in this research, the sheep blood was washed (defibrinated) with NaCl 0.85%. The purpose of the research is to determine the effect of washed sheep blood with NaCl 0.85% on the growth of Neisseria gonorrhoeae in CAP. The method of this research is a pre-experiment conducted in a laboratory to find out a symptom or effect that arises as a result of the treatment of the sample. The treatment in this research is CAP washed of sheep blood and CAP with unwashed sheep blood as a control. The result is the colonies that grew on CAP treatment were better than colonies that grew on CAP control. The conclusion of this research is there is an effect of washed sheep blood with NaCl 0.85% on the growth of Neisseria gonorrhoeae in CAP medium.
Hubungan Kadar Kreatinin Dengan Hemoglobin Pada Pasien Gagal Ginjal Kronik Sebagai Rujukan Terapi Erythropoietin (EPO) Tandi Manu, Thomas; Zaena Safitri, Trysia; Urip, Urip; Pauzi, Iswari; Khusuma, Ari
Indonesian Health Issue Vol. 4 No. 2 (2025): AGUSTUS
Publisher : Indonesian Journal Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47134/inhis.v4i2.132

Abstract

Background: Chronic kidney disease (CKD) is a common condition and a global health problem with various causes that lead to a gradual decline in kidney function, resulting in increased creatinine levels. This condition reduces the production of the hormone erythropoietin (EPO), which stimulates the bone marrow to produce red blood cells, leading to decreased hemoglobin levels and resulting in anemia. Erythropoietin (EPO) therapy represents a primary therapeutic option for the management of anemia in patients with chronic kidney disease (CKD) PurposeTo determine the relationship between creatinine and hemoglobin levels in chronic kidney disease patients as a reference for erythropoietin (EPO) therapy. Methods: This study employed an analytical observational method with a cross-sectional approach by reviewing medical record data. The sample consisted of 56 patients obtained through total sampling. The data were analyzed using the Pearson correlation test. Results: The average creatinine level was 9.92 mg/dL, and the average hemoglobin (Hb) level was 7.96 g/dL. Pearson correlation analysis between creatinine and hemoglobin levels in patients receiving erythropoietin (EPO) therapy showed a statistically significant result with a p-value of 0.001 (<0.05) and a moderate negative correlation (r = -0.436). Conclusion: A significant negative correlation was found between creatinine and hemoglobin levels in patients undergoing EPO therapy, indicating that higher creatinine levels tend to be associated with lower hemoglobin concentrations.
The Effect Of Duration Of Tuak Consumption On Low Density Lipoprotein (LDL) And Triglyceride (TG) Levels In The Community In Taman Bali Village Manu, Thomas Tandi; Ananda, Dewa Ayu Putu Wiweka; Getas, I Wayan; Wiadnya, Ida Bagus Rai; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.544

Abstract

Tuak is a traditional alcoholic drink made from palm sap. Excessive alcohol consumption over a long period of time can affect lipid metabolism, increasing LDL and Triglyceride levels in the blood. Increased levels can cause cardiovascular disease. To determine the effect of the duration of tuak consumption on LDL and Triglyceride levels in the community in Dusun Taman Bali. The research used is Observational Analytical with a cross-sectional approach. The sample in this study was the community in Dusun Taman Bali who consumed Tuak for 1, 3, and 5 years with a total of 30 respondents using One Way Anova data analysis. The average LDL levels of 30 respondents with an average of 117.20 mg/dL consumed tuak for 1 year, 130.30 mg/dL for 3 years and 151.20 mg/dL for 5 years, while the average Triglyceride levels of 30 respondents who consumed Tuak for 1 year were 160.90 mg/dL, 166.60 mg/dL for 3 years and 201.10 mg/dL for 5 years. The results obtained showed a significant value of LDL levels of 0.000 while Triglyceride levels were 0.022. This means P <0.05, which means that there is an effect of the duration of tuak consumption of 1.3 and 5 years on LDL and Triglyceride levels in the community in Dusun Taman Bali.
Bacterial Contamination Analysis Based On Total Plate Count From Various Uses Of Compact Powder Putri, Ni Putu Sintyani; Kristinawati, Erna; Khusuma, Ari
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.470

Abstract

The repeated and alternating use of compact powder can increase the risk of bacterical contamination. This contamination can be harmful to the skin health of the users, especially if the compact powder used has been contamination with bacteria exceeding the standard limit set by BPOM, which is 5 × 10³ colonies/g or colonies/mL. Therefore, an analysis of bacterial contamination in the repeatedly and alternately used sompact powder from various uses is necessary using the Total Plate Cound (TPC) method to ensure the suitability and safety of the compact powder. The objective is to determine the level of bacterial contamination based on the Total Plate Cound (TPC) on compact powder used by makeup artists, tester from cosmetic stores, personal use dan new product. The samples of conpact powder analyzed amounted to 24 samples, using purposive sampling technique and the data was analyzed descriptively. The result obtained from the calculation of the average number of bacterical colonies in personal use compact powder, new product and tester from each cosmetic store are < 3 x 10¹ CFU/g, while the average for compact powders sourced from makeup artists is 1.66 x 10^5 CFU/g. Compact powder derived from personal use, new products, and those used as testers by cosmetic stores show ALT values that still fall within the category of meeting the standards set by BPOM. Meanwhile, compact powder derived from makeup artists shows ALT values that exceed the standards set by BPOM.  
Long Suffering Type 1 Diabetes Mellitus During Metabolic Acidosis on Positiveness of Ketones in Urine Patients Mahayani, I Gusti Ayu Putu Sachita; Urip, Urip; Khusuma, Ari; Pauzi, Iswari
THRIVE Health Science Journal Vol. 1 No. 1 (2024): March
Publisher : Balai Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56566/thrive.v1i1.89

Abstract

Examination of ketones in the patient's urine and measuring the patient's blood sugar level will determine whether there will be metabolic acidosis in patients with type 1 diabetes mellitus, which is a metabolic disorder that can be caused by various etiologies, such as chronic hyperglycemia due to impaired insulin secretion or impaired insulin action. alone. Diabetes Mellitus type 1 itself is more caused by reduced insulin secretion due to damage to pancreatic -cells. This study was conducted to examine ketones in urine on the duration of suffering from type 1 diabetes mellitus when metabolic acidosis occurs Data collection in this study was carried out by accidental sampling method by researchers to collect secondary and primary data on urine ketones in patients with metabolic acidosis. Based on research that has been done, data obtained from 34 patients with type 1 diabetes mellitus at the Sigerongan Health Center, as many as 8 patients with positive results of urine ketones with a prevalence of +2 totaling 3 people and +3 totaling 5 people. Judging from the high level of glucose (hyperglycemia) that is > 200 mg/dl and the positivity of ketone bodies in the patient's urine as a sign of metabolic acidosis. With a long-susceptibility to suffer from diabetes mellitus 1-5 years and above. The results of measuring ketone levels in the patient's urine show that there is no relationship with the duration of suffering from diabetes mellitus in the urine but blood sugar levels.
Differences in Cholesterol Levels in Coffee Drinkers Without Sugar and Coffee Drinkers With Sugar in The Work Area Salsabila, Nadira; Khusuma, Ari; Jiwintarum, Yunan
THRIVE Health Science Journal Vol. 1 No. 1 (2024): March
Publisher : Balai Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56566/thrive.v1i1.93

Abstract

Indonesia is one of the coffee-producing countries as well as coffee bean exporters which ranks fourth in the world. Coffee is one of the most consumed beverages in the world which has become a major need for people without realizing it. Based on the results of previous studies that coffee drinkers using sugar can increase cholesterol levels. Excessive cholesterol in the body will accumulate in the walls of blood vessels and cause a condition called atherosclerosis, namely narrowing or hardening of the arteries. This condition is the forerunner of heart disease and stroke. This study aims to determine differences in cholesterol levels in coffee drinkers without sugar and coffee drinkers using sugar in the working area of Karang Pule. This research is an analytical observation using capillary blood samples of respondents who drink coffee without sugar and drink coffee with sugar. The data obtained in this study were recorded and presented in tabular form and analyzed descriptively. From the results of the study, it was found that the results of examining cholesterol levels in coffee drinkers without sugar were 15 people with an average cholesterol level of 169 mg/dl. Meanwhile, coffee drinkers using sugar were 15 people with an average cholesterol level examination of 242 mg/dl
Co-Authors Agrijanti Agrijanti, Agrijanti Ananda, Dewa Ayu Putu Wiweka Arfi, Sofiyan Dwi Astriani, Astriani Cahayaningrum, Aryani Dewi, Lale Budi Kusuma Eustachius Hagni Wardoyo Fakhmi, Shausan Nabila Getas, I Wayan Intan Suryawatie Yomo, Meidiana Kadek Sinta Cahyani, Ni Diah Kristinawati, Erna Laila Nisa, Isra Lalu Srigede, Lalu Mahayani, I Gusti Ayu Putu Sachita Manu, Thomas Tandi Novianty, Alfiah Nurul Inayati Pancawati Ariami Pauzi, Iswari Permatasari, Lina Putri, Ni Putu Sintyani Reginata, Ni Luh Vira Rohmi Rohmi, Rohmi Salsabila, Nadira Siti Zaetun, Siti Tandi Manu, Thomas Urip Urip, Urip Wiadnya, Ida Bagus Rai Wulandari, Ayu Nurislami Yunan Jiwintarum Yusari, Lala Deswinta Zaena Safitri, Trysia