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Khusuma, Ari
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Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Perbedaan Jumlah Limfosit dan Monosit serta Morfologi Eritrosit pada Perokok Tembakau dan Elektrik sebagai Faktor Risiko Aterosklerosis pada Remaja Laila Nisa, Isra; Rohmi; Khusuma, Ari; Zaetun, Siti
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.60

Abstract

Background : Smoking from adolescence can increase the risk of atherosclerosis because there is a buildup of fibrolipids and other substances in the blood that can cause plaque. Meanwhile, research results show that e-cigarettes are no less dangerous than tobacco cigarettes. Namely, the number of lymphocytes in e-smokers is 54% and tobacco smokers are 39% (Mega, 2020). The novelty of this research is to look at differences in erythrocyte count and morphology in adolescents at risk of atherosclerosis. Research Objectives : To determine the differences in the number of lymphocytes and monocytes and the morphology of erythrocytes in tobacco and electronic smokers as risk factors for atherosclerosis in adolescents. Research Methods : This research is an analytical observational study with a cross sectional research design and uses the lameshow formula to determine the sample size so that a sample size of 30 samples can be obtained. Data were tested using the Independent T-test and Mann Whitney-U test. The results of the study : The average percentage of lymphocytes and monocytes in tobacco and electronic smoker is 37% & 27% lymphocytes and 7% & 6% monocytes. From 15 samples of tobacco smokers the results were normal and from 15 samples of e-smokers there was 1 lymphocyte result below normal. Also, the abnormal cells found include anulocytes, tear drops, burr cells, ovalocytes and stomatocytes. Conclusion : There are differences in the number of lymphocytes and monocytes as well as erythrocyte morphology in tobacco and electronic smokers as risk factors for atherosclerosis in adolescents with the probability value for lymphocytes being 0.01<0.05 and monocytes 0.04<0.05.
Identifikasi Sel Ragi (Candida sp.) pada Sedimen Urine dengan Menggunaka Metode Preparat Basah dan Preparat Gram pada Penderita Diabetes Melitus Cahayaningrum, Aryani; Agrijanti; Manu, Thomas Tandi; Khusuma, Ari
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.75

Abstract

Latar Belakang: Kandidiasis adalah infeksi jamur yang disebabkan oleh jamur Candida sp. Diabetes melitus menjadi salah satu faktor predisposisi terjadinya infeksi jamur. Diagnosa dari kandidiasis bisa dilakukan dengan berbagai macam cara. Salah satunya adalah dengan melihat sel ragi pada sedimen urin di bawah mikroskop dengan menggunakan metode preparat basah atau melakukan pewarnaan gram . Tujuan Penelitian: Mengidentifikasi sel ragi (Candida sp.) pada sedimen urin dengan menggunakan metode preparat basah dan preparat gram pada penderita Diabetes Melitus. Metode Penelitian: Rancangan Penelitian ini merupakan penelitian deskriptif, yang dilakukan dengan metode observasional analitik dengan pendekatan cross sectional sehingga variabel bebas dan variabel terikat diamati dalam satu waktu. Hasil: Didapatkan 8 sampel positif sel ragi dan 14 sampel negatif sel ragi pada metode preparat basah dan preparat gram. Kesimpulan: Berdasarkan kualitas, preparat gram lebih unggul daripada preparat basah karena sel ragi yang terlihat pada preparat gram lebih jelas daripada sel ragi yang terlihat pada preparat gram. Sedangkan dari segi efisensi waktu dan biaya, preparat basah lebih unggul karena cepat dan murah.
Co-Authors Agrijanti Agrijanti, Agrijanti Ananda, Dewa Ayu Putu Wiweka Arfi, Sofiyan Dwi Astriani, Astriani Cahayaningrum, Aryani Dewi, Lale Budi Kusuma Eustachius Hagni Wardoyo Fakhmi, Shausan Nabila Getas, I Wayan Intan Suryawatie Yomo, Meidiana Kadek Sinta Cahyani, Ni Diah Kristinawati, Erna Laila Nisa, Isra Lalu Srigede, Lalu Mahayani, I Gusti Ayu Putu Sachita Manu, Thomas Tandi Novianty, Alfiah Nurul Inayati Pancawati Ariami Pauzi, Iswari Permatasari, Lina Putri, Ni Putu Sintyani Reginata, Ni Luh Vira Rohmi Rohmi, Rohmi Salsabila, Nadira Siti Zaetun, Siti Tandi Manu, Thomas Urip Urip, Urip Wiadnya, Ida Bagus Rai Wulandari, Ayu Nurislami Yunan Jiwintarum Yusari, Lala Deswinta Zaena Safitri, Trysia