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Journal : Journal of Applied Veterinary Science and Technology

Total Lactic Acid Bacteria (LAB) and Antioxidant Activity of Goat's Milk Yoghurt with The Addition Of Red Bran During Refrigeration Storage Jangnga, Indah Puspadyah; Haskito, Ajeng Erika Prihastuti; Sari, Citra; Adrenalin, Sruti Listra
Journal of Applied Veterinary Science And Technology Vol. 4 No. 2 (2023): October 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V4.I2.2023.61-66

Abstract

Background: Goat milk yogurt is a functional food made from processed food of animal origin with the help of LAB fermentation, which is healthy for the body. The utilization of red rice bran flour as an additional ingredient in making yogurt can increase the level of antioxidant activity and become a food fiber content in yogurt. Purpose: This study aims to determine the effect of refrigerated storage temperatures (4℃) of goat milk yogurt with the addition of red rice bran flour on total LAB and antioxidant activity. Methods: Using a CRD with five treatments and four replications. The treatment included 0, 1, 2, 3, and 4 weeks of storage. Each treatment group will be calculated for total LAB using the TPC method and antioxidant activity testing using the DPPH method. The data obtained were analyzed using the One Way ANOVA Test followed by the Duncan Test to determine the effect between treatment groups. Results: The results showed that storage time significantly affected (p<0.01) total LAB and antioxidant activity. The results of total LAB successively from 0 to 4 weeks of storage were 8,0 x 108 CFU/ml, 7,8 x 107 CFU/ml, 7,6 x 107 CFU/ml, 6,4 x 106 CFU/ml, and 6,3 x 106 CFU/ml. The results of antioxidant activity successively from 0 to 4 weeks of storage were 534,29 mg/ml, 443,96 mg/ml, 358,61 mg/ml, 282,32 mg/ml, and 230,88 mg/ml. Conclusion: Goat's milk yogurt with the addition of brown rice bran flour is recommended to be consumed for up to 2 weeks of storage. 
Occurrence and Antimicrobial Resistance Profiles of MRSA and ESBL-Producing Enterobacteriaceae in a Veterinary Hospital Environment Syah, Ali Akram; Dameanti, Fidi Nur Aini Eka Puji; Effendi, Mustofa Helmi; Amri, Indah Amalia; Adrenalin, Sruti Listra; Luthfiana, Nurul
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.109-117

Abstract

Background: Nosocomial infections pose a major challenge not only in human hospitals but also in veterinary settings. These infections are frequently associated with methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E). Purpose:  This study aimed to detect and characterize MRSA and ESBL-E isolated from environmental samples at the Veterinary Teaching Hospital, Universitas Brawijaya (VTH UB). Method: The study involved four main procedures: (1) sample isolation; (2) bacterial identification of S. aureus and Enterobacteriaceae using MacConkey agar supplemented with 1 mg/L cefotaxime (MCA + 1 mg/L); (3) antibiotic susceptibility testing and MRSA confirmation using Oxacillin Resistance Screening Agar Base (ORSAB); and (4) confirmation of ESBL production through the Double Disk Synergy Test (DDST). Results: Isolation and identification revealed S. aureus in 86.6% (13/15) of samples and Enterobacteriaceae in 26.6% (4/15). Among Enterobacteriaceae, Klebsiella pneumoniae accounted for 6.7% (1/15) and Enterobacter aerogenes for 20% (3/15). Antimicrobial resistance testing of Staphylococcus aureus isolates showed that all (13/13) were resistant to at least one antibiotic class. Resistance rates were 53.8% (7/13) to oxacillin, 92.3% (12/13) to cefoxitin, 23.0% (3/13) to tetracycline, 92.3% (12/13) to erythromycin, and 46.1% (6/13) to gentamicin. The prevalence of MRSA among environmental isolates was 46.6% (7/15), while ESBL-E prevalence was 26.6% (4/15). Conclusion: The detection of MRSA and ESBL-E in the hospital environment indicates potential zoonotic and hospital associated infection risks in veterinary facilities. These findings emphasize the importance of strengthened infection control practices and provide a baseline for broader surveillance across veterinary hospitals.