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Journal : Jurnal Veteriner

Efikasi Vaksinasi pada Benih Ikan Nila (Oreochromis niloticus) dengan Metode Infiltrasi Hiperosmotik untuk Mencegah Infeksi Streptococcus agalactiae (THE EFFICACY OF VACCINATION ON TILAPIA SEEDS OF (Oreochromis niloticus) USING HYPEROSMOTIC INFILTRATION Amalia Putri Firdausi; Sukenda Sukenda; Sri Nuryati
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.123 KB) | DOI: 10.19087/jveteriner.2017.18.4.634

Abstract

The objective of this study was to evaluate the effectiveness of vaccination on tilapia seedlings resulted from the vaccinated parent by hyperosmotic infiltration method at four different salinity to prevent Streptococcus agalactiae bacterial infection. A total of 100 seeds aged 20 days from the vaccinated mother were immersed in four different salinity: 0 ppt (control), 10 ppt, 20 ppt, and 30 ppt for five minutes, then removed and transferred into vaccine-containing containers for 30 minutes. Further maintenance was performed in freshwater aquariums and at days 10, 20, and 30 post vaccination, the immune system performance: Relative Percent Survival (RPS) after the challenge test, specific antibody level, and lysozyme was observed. The study was conducted using a complete randomized design with three replications. The results showed that the 10 ppt salinity treatment gave the best results compared to the others and control. The final RPS (10 ppt) value was 84.72%, 66.49%, and 47.06%, on the 10th, 20th, and 30thh days of vaccination, respectively. Specific antibody levels (10 ppt) were 0.077, 0.078, and 0.077 and lysozyme 0.092, 0.084, and 0.032 at days 10, 20, and 30 post vaccination, respectively. The value of RPS and specific antibody level at 10 ppt salinity was significantly different (P <0.05) compared to the other treatments, while the lysozyme treatment activity of 10 ppt was not significantly different compared to the 20 ppt treatment. Vaccination using 10 ppt saline hyperosmotic infiltration can improve the performance of the immune system by improving maternal immune protection against Streptococcus agalactiae infection.
Co-Authors , Rahman, , Abrisam Hadi Bintoro Achmad Zidan Akmal Maulana Adna Sumadikarta Adna Sumadikarta Adycha, Pricila Aurora Alhaq, ⁠Sofyan Anassyahtira, Aliffia Andri Iskandar Aulia Herlita Bintan Naktavia Bintoro, Abrisam Hadi Cecilia Eny Indriastuti Choerunnisa, Hasna Najla Dian Eka Ramadhani Fachrezi, Muhammad Hafidz Fika Ismaladewi Filibertus Tantio Firdaus, Mujammad Ridlo Firjatullah, Muhammad Sahlan Hendriana, Andri Herlita, Aulia Hikmal Yumilzam Ihsan, Muhammad Fajar Maulana Ima Kusumanti Indriastuti, Cecilia Eny Iqbal Kurniawinata, Mohamad Ishaq, ⁠Maulana Iskandar, Andri Ismaladewi, Fika Jemmy Erwanda Maulana Khalisha, Nadira Lesmana, Dudi M. Isya Rais A. Maha, Indah Permata Sari Br Maha, Indah Permatasari Mauladina, Annas Yudha Maulana, Achmad Zidan Akmal Maulana, Jemmy Erwanda Muarif Muarif Muhammad Aqshol Dafa Ramadhan Muhammad Arif Mulya Muhammad Sahlan Firjatullah Mulanti, Rahmah Mulya, Muhammad Arif MUNTI YUHANA Nadira Khalisha Nainggolan, Zacky Naoval Naktavia, Bintan Permatasari, Sheny Pradian, Yassar Ariq Prama, Ega Adtiya Pratiwi, Rifqah Priatna, Moh. Fahri Aulia Priatna, Mohamad Fahri Rahmah Mulanti Rahman Rahman Rahman, Riem Rahayu Rais A., M. Isya Ramadhan, Muhammad Aqshol Dafa Ramadhani, Dian Eka Rangkuti, Gavrilan Hafiz Ranta Sumadi Respati Mahadhika Reta Sagita Riem Rahayu Rahman Risma Arafah Tunisa Rivasa, Varel Raihan Rizky, M. Fizry Alnur Sagita, Reta Sasmita, Galih Amar Taufiqurrahman Satrio Yudha Wisesa Sheny Permatasari Sopacua, Ivana Oktarina Sri Nuryati Sukenda Sukenda Surahman, Nur Prasetyo Ari Syafira, Nadia Tunisa, Risma Arafah Tunisah, Risma Arafah Wahyudi, Imam Tri Wida Lesmanawati Wildan Nurussalam Wiyoto Wiyoto Wiyoto Wiyoto, Wiyoto Yumilzam, Hikmal Zacky Naoval Nainggolan ⁠Maulana Ishaq ⁠Sofyan Alhaq