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Fanata, Wahyu Indra Duwi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Materials Science & Nanotechnology Mathematics Physics

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Journal : Biosaintifika: Journal of Biology

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Molecular Detection of Rust Fungus (Puccinia spp.) on Sugarcane using ITS region Tanzil, Ahmad Ilham; Maulida, Dinda Mala; Fanata, Wahyu Indra Duwi; Neliana, Intan Ria; Sugiharto, Bambang; Magnon, Cassidy
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): Inpress December 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.32946

Abstract

Identifying pathogenic fungi at the species level based on morphology, especially for leaf rust, is quite difficult. Further investigation, such as molecular techniques, is required to identify leaf rust fungi precisely. One way that can be employed is DNA barcoding. This study seeks to detect leaf rust fungi (Puccinia spp.) on sugarcane. Accurate species identification allows early detection of Puccinia kuehnii, helping farmers and extension agencies to apply targeted control strategies. A fungal sample was collected from sugarcane fields in Bondowoso, East Java, Indonesia. Morphological identification was done with an SEM, whereas molecular identification was done through DNA extraction, amplification, visualization, sequencing, and phylogenetic tree creation. The primers employed are obtained from the ITS region of ribosomal DNA, which contains enough diversity to differentiate fungi at the species level. The amplification findings revealed a DNA band of 500 bp at both loci. Sequencing results indicate that samples The sequencing results indicatethat the samples from Bondowoso are closely related to the Puccinia kuehnii sequence from NCBI. The findings of the phylogenetic tree construction revealed that samples from both locations are still associated with P. kuehnii sequences from other nations. This is the first study documenting P. kuehnii in Indonesia, especially East Java, that integrates morphological and molecular characterization.
Co-Authors . Usmadi Adie, Moch Muchlish Ahmad Ilham Tanzil Ali Wafa Ariyono, Alief Rizky Arum, Ayu Puspita Asshidiqie, Wahyu Pratama Bambang Sugiharto Budi Hartono HARDIAN SUSILO ADDY Hardiyani, Wulan Arum Hari Purnomo Husna, Syafira Fatihatul Ika Purnamasari Intan Kartika Setyawati Khamidah, Khusna Luh Putu Suciati Magfiroh, Illia Seldon Maghfirah, Intan Hadiatun Magnon, Cassidy Maulida, Dinda Mala Muhammad, Adibillah Muhtadi, Much. Misbah Nafiah, Nur Lailin Neliana, Intan Ria Nikmah, Nasilatun Nisa, Aminatun Novitasari , Adin Nur Widodo Parawita Dewanti Puji Rahayu Raharja, Ajung Gilang Putra Ramadhan, Galang Rizki Ramadhani, Rifki Fahrisal Ratnasari, Tri Refa Firgiyanto Restanto, Didik Pudji Ristiyana, Suci Saydi, Royhan Setiawan, Dedi Edo Setiyono Setiyono Setyadi, Farhad Dwi Sholikhah, A’idah Mar’atus Subekti, Andri Oktavian Syafina Pusparani Taufik, Rahadian Falqi TRI HANDOYO Tri Wahyu Saputra Ummah, Anisatul Ummi Sholikhah Utami, Sri Rejeki Wahyu Nurkholis Hadi Syahputra Yusuf Rachmandika