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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Caraka Tani: Journal of Sustainable Agriculture Biosaintifika: Journal of Biology & Biology Education PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Darmabakti Cendekia: Journal of Community Service and Engagements Journal Pharmasci (Journal of Pharmacy and Science) Sewagati Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Yosephine Sri Wulan Manuhara
Department Of Biology, Faculty Of Science And Technology, Airlangga University, Surabaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Optimization of Biomass and Secondary Metabolite Production in Gynura procumbens (Lour.) Merr. Adventitious Roots Culture by Using the Method of Subculture and Fed-batch Cultivation in a Bioreactor Dannis Yuda Kusuma; Alfinda Novi Kristanti; Anjar Tri Wibowo; Boon Chin Tan; Yosephine Sri Wulan Manuhara
HAYATI Journal of Biosciences Vol. 30 No. 5 (2023): September 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.5.797-807

Abstract

The valuable extract of bioactive compounds from Gynura procumbens has been widely manufactured into various health products. The demand for these compounds is continuously increasing, but production through conventional farming methods is insufficient due to limited agricultural land and environmental stresses. An alternative to producing plant biomass is in vitro cultivation methods. This method requires less space and enables biomass propagation in a controlled condition that can facilitate stable and efficient production of plant secondary metabolites. This study evaluated the effect of inoculum subculture periods and culture methods on G. procumbens biomass and secondary metabolite production in a bioreactor. The 3-L airlift balloon type-bubble bioreactors was modified in this study to adopt the treatment of 1st-5th subculture periods and fed- and batch-cultivation strategies. We found the G. procumbens adventitious root culture was optimally derived from the 1st subculture produced biomass of 148.02±1.45 g FW and 8.59±0.12 g DW, and TPC (14.48±1.08 mg GAE/g DW) and TFC (116.89±0.44 mg KE/g DW and 33.97±0.13 mg QE/g DW). Additionally, the fed method after 28 days of culture using double distilled water replenishment improved adventitious root biomass (213.75±35.00 g FW and 11.21±0.18 g DW), while nutrient replenishment improved TFC (52.14±0.44 mg KE/g DW and 14.54±0.13 mg QE/g DW). These results can be used to optimize the cultivation of G. procumbens adventitious roots in a large-scale bioreactor.
Biomass Production of Gynura procumbens Adventitious Roots in Different Type of Liquid Culture Yosephine Sri Wulan Manuhara; Dannis Yuda Kusuma; Rafika Lailiyatul Kurnia Sari; Alfinda Novi Kristanti
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.9670

Abstract

Gynura procumbens has a potency to produce raw material for pharmaceutical industry. Liquid culture systems have significant effects on multiplication rates of organ, so the propagation technology to increase biomass of this plant or organ using the liquid culture system was necessary. This research was conducted to compare biomass production of adventitious roots of G. procumbens in different liquid culture (shake flask, temporary immersion bioreactor and balloon-type bubble bioreactor). Adventitious roots culture was maintained in Murashige and Skoog liquid medium supplemented with IBA (indole butyric acid) 5 mg/L and different concentrations of sucrose (10, 30, 50 g/L). Result showed that the highest biomass production (fresh weight) was in shake flask that was 3.9-fold higher than initial explant, while in temporary immersion system was 5.12-fold higher than initial explant, and in balloon- type bubble bioreactor was 13.1-fold higher than initial explant. The highest enhancement of adventitious roots was occurred at sucrose supplementation of 50 g/L. Based on this research we found that the best type of liquid culture to increase biomass of G. procumbens adventitious roots was balloon-type bubble bioreactor. Scaling-up of adventitious root culture are necessary to fulfill the need of raw material for pharmaceutical industry and ballon-type bubble bioreactor was the most suitable method to scale up the performance. 
Budidaya Cabai Besar di Lahan Tidak Produktif Desa Keputih, Kecamatan Sukolilo, Kota Surabaya dengan Teknik Hidroponik Sistem Irigasi Tetes: Upaya Memberdayakan Kelompok Ibu-ibu PKK Sugiarso, Djarot; Juwono, Hendro; Harmami, Harmami; Ulfin, Ita; Ni'mah, Yatim Lailun; Manuhara, Yosephine Sri Wulan
Sewagati Vol 8 No 3 (2024)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v8i3.947

Abstract

Budidaya cabai besar menggunakan teknik hidroponik sistem irigasi tetes diterapkan dalam kegiatan Pengabdian kepada Masyarakat (PkM) di Kelurahan Keputih, Kecamatan Sukolilo, Kota Surabaya dengan latar belakang adanya lahan tidak produktif di desa tersebut dan Ibu-ibu PKK sebagai mitra. Teknik penanaman menggunakan metode hidroponik dapat mengatasi kondisi lahan yang tidak subur, karena metode ini menggunakan nutrisi yang terlarut dalam media air, sehingga tidak membutuhkan media tanam padat (tanah). Budidaya cabai besar dilakukan di dalam rumah kaca ukuran 6 x 12 m (72 m2) agar tanaman tidak terpapar hama. Kerangka rumah kaca terbuat dari kanal c75 galvalum. Atap rumah kaca adalah plastik UV tebal 250 µ, 14%; sedangkan untuk menutup rumah kaca digunakan insect net. Selanjutkan kegiatan PkM dilanjutkan dengan pelatihan dan praktek hidroponik system irigasi tetes. Hasil kegiatan pengabdian kepada Masyarakat menunjukkan keterlibatan Ibu-ibu PKK dalam seluruh kegiatan dan hasil budidaya cabai besar menghasilkan cabai besar yang layak jual, sehingga diharapkan pengetahuan yang diberikan dalam kegiatan pengabdian kepada Masyarakat ini dapat meningkatkan pendapatan.
Methyl Jasmonate Elicitation Enhanced Biomass, Phenolic, and Flavonoid Production of Adventitious Root Culture of Gynura procumbens in Balloon Type Bubble Bioreactor Wijayanti, Ely Tri; Kusuma, Dannis Yuda; Kristanti, Alfinda Novi; Wibowo, Anjar Tri; Sugiharto, Sugiharto; Sugiarso, Djarot; Manuhara, Yosephine Sri Wulan
Majalah Obat Tradisional Vol 29, No 2 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.87736

Abstract

Secondary metabolites in the medicinal plant Gynura procumbens have various beneficial pharmacological activities. However, the limited availability of these beneficial compounds has constrained the possibilities for therapeutic applications. Exogenously applied methyl jasmonate (MeJa) as an elicitor has been reported to trigger the accumulation of secondary metabolites in plant tissues cultured in vitro. This study aimed to assess how varying periods of MeJa elicitation affect the growth and accumulation of phenolic and flavonoid compounds in adventitious root cultures of G. procumbens within a balloon-type bubble bioreactor (BTBB). Adventitious roots were induced from leaf and stem explants obtained from established in vitro shoots. Upon transfer to the BTBB, the cultures were supplemented with 100 µM MeJa and sampled at 1, 2, 3, and 4 weeks. The results revealed that including MeJa in the culture medium significantly boosted growth and the accumulation of phenolic and flavonoid compounds in G. procumbens. The highest biomass and concentrations of phenolic and flavonoid compounds were observed after 3 weeks of elicitation. These findings highlight the potential of MeJa elicitation for enhancing the production of valuable bioactive compounds in G. procumbens adventitious root cultures, showcasing its utility for industrial applications.
Evaluation of Temperature Stress Under Different Hydroponic Systems on Growth and Saponin Content of Talinum paniculatum Gaertn. Cuttings Yachya, Arif; Kristanti, Alfinda Novi; Manuhara, Yosephine Sri Wulan
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84241

Abstract

Increases in the temperature of nutrient solutions have restricted the use of hydroponic cultivation in the tropics, predominantly due to plant stress. This study aimed to evaluate the effects of temperature stress under different hydroponic systems on the growth and saponin content of Talinum paniculatum cuttings. Three hydroponic systems, i.e., deep flow technique (DFT), nutrient film technique (NFT), and aeroponic, were tested. The temperature of the nutrient solution was set for each system, i.e., under ambient temperature (UAT) and with controlled temperature (WCT) at 26° C. The cultivation period was 60 days. The result showed peroxidation activity and proline accumulation for the adventitious roots of T. paniculatum cuttings with UAT and WCT, alongside various levels of plasma membrane damage. Levels of Malondialdehyde (MDA) and proline were analyzed by spectrophotometer. Membrane damage was analyzed with Evans blue dye. The results indicated that the levels of MDA and proline accumulation under the three hydroponic systems were higher for the WCT than for the UAT treatment. In contrast, vegetative growth was higher in UAT than in WCT. The saponin content of the adventitious root correlated with the MDA level. Saponin production was triggered by oxidative stress during cultivation, while the adventitious roots had a higher saponin content in all three hydroponic systems with the WCT treatment compared to the UAT treatment. Among the systems, aeroponic was superior for biomass and saponin. Root growth was promoted in the nutrient solution under ambient temperature whereas the production of saponins was stimulated under the controlled temperature. In the aeroponic system, root biomass values of 1.17 and 0.478 g dry weight were obtained under ambient and controlled temperatures, respectively. The total saponin contents differed slightly, namely 189.83 and 195.61 mg/g, respectively. 
Amorphophallus muelleri Blume Shoot Induction on Different Media Types and Plant Growth Regulator Combinations Oktavia, Gebby Agnessya Esa; Junairiah, Junairiah; Manuhara, Yosephine Sri Wulan
AGRIVITA Journal of Agricultural Science Vol 46, No 3 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i3.4477

Abstract

Conventional propagation experiences problems due to long period for seed production, disease contamination, low reproductive rate and season dependent. In vitro propagation offers several advantages. This research aims to assess the impact of different media types and the concentrations of BAP and IBA on the in vitro growth and development of A. muelleri shoots. Bulbil explants were cultivated using MS media with different media types (solid media, agitated liquid media, and static liquid media) with various BAP and IBA concentration treatments (1 mg/l, 2 mg/l, and 3 mg/l). The results demonstrated that the media type and combinations of BAP and IBA treatments significantly influenced period of callus formation and the number of shoots. The development of explant on both solid and static liquid media involves initial explant swelling, formation of nodular callus, greenish buds, and ultimately shoots. Anatomical observation showed that explants experienced indirect organogenesis, characterized by the presence of pre meristem nodular callus, meristematic tissue, apical meristem and leaf primordia. The agitated liquid media didn’t promote growth and development of the explants. The most effective combination was found to be the static liquid media supplemented with 3 mg/l BAP and 1 mg/l IBA.
Produksi Cabai Melalui Teknik Hidroponik Irigasi Tetes untuk Meningkatkan Pendapatan Komunitas Pemuda Tentena, Kabupaten Poso, Sulawesi Tengah Manuhara, Yosephine Sri Wulan; Wibowo, Anjar Tri; Sugiharto, Sugiharto; Sugiarso, Djarot
Sewagati Vol 9 No 3 (2025)
Publisher : Pusat Publikasi ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j26139960.v9i3.2322

Abstract

Kota Tentena adalah salah satu pusat wisata dengan adanya danau Poso yang sangat terkenal. Namun masih terdapat  masyarakat yang hidup miskin. Kelompok pemuda Pukat Tentena di desa Tentena, Kabupaten Poso merupakan kelompok yang mengarah pada ekonomi produktif yang dalam beberapa tahun ini memiliki usaha pertanian sayur dan buah secara hidroponik untuk menampung pemuda dari daerah pelosok yang ingin melanjutkan sekolah namun tidak memiliki biaya. Namun masih banyak pemuda yang belum dapat dibantu untuk membiayai sekolah mereka. Sementara itu Komunitas Pemuda masih memiliki lahan yang tidak produktif. Oleh karena itu melalui program Kemitraan Masyarakat ini dilakukan kegiatan untuk memanfaatkan lahan tidak produktif tersebut sebagai lahan produksi cabai melalui teknik hidroponik irigasi tetes. Metode pelaksanaan dilakukan dalam beberapa tahap yaitu pembuatan rumah kaca (green house), pelatihan budidaya cabai menggunakan teknik hidroponik sistem irigasi tetes, penanaman benih cabai, pembuatan hidroponik sistem irigasi tetes, pemindahan dan pemeliharaan bibit cabai dalam sistem irigasi tetes.Setelah dipelihara selama lebih kurang 60 hari tanaman mulai berbunga dan berbuah. Untuk pemanenan cabai bisa dilakukan beberapa kali. Pada panen perdana ini diperoleh lebih kurang 250 g per tanaman. Hasil penjualan cabai dapat menambah pendapatan pemuda Tentena sebagai sasaran Mitra Pengabdian.
Expression of the curculin gene from different organs of Curculigo latifolia under different culture conditions Muslihatin, Wirdhatul; Fajar, Nur Syamsiatul; Wibowo, Anjar Tri; Manuhara, Yosephine Sri Wulan
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.1131

Abstract

Curculigo latifolia is the main source of curculin. Curculin is a special kind of protein that tastes sweet and can change the way food tastes, and is naturally expressed in fruit. This study aims to measure and compare the relative expression of the curculin gene in different organs, i.e., fruit and leaf, under in vitro and in vivo conditions. In this study, mRNA isolation was carried out in tissues derived from fruits and leaves grown in vitro and in vivo (in the soil) of C. latifolia from West Java, Indonesia. Leaves from 20 weeks seedling on polybag, fruit 40 days after anthesis, and leaves from 20 weeks seedling of C. latifolia cultured on MS0 medium that were used in this experiment. The relative expression was measured using qRT-PCR. The results showed that the comparison of curculigo’s parts in the leaves was lower than in the fruit. The lowest expression was obtained in leaves grown under in vitro conditions at 0.001-fold, while leaves grown in the soil at 0.566-fold compared to curculin gene expression in fruit. This study concludes that the expression of the Curculin gene in fruit is different between the fruit and leaves, and the growth conditions have an influence on Curculin gene expression, where Curculin gene expression under in vitro conditions is lower than expression under in vivo conditions. The curculin is not only expressed in the fruit but also the leaves, so it has the potential to be developed.
Induksi Kalus Amorphophallus titanum (Becc.) Becc. Melalui Kultur In Vitro: Callus Induction of Amorphophallus titanum (Becc.) Becc. Through In Vitro Culture Dannis Yuda Kusuma; Anjar Tri Wibowo; Yosephine Sri Wulan Manuhara
Jurnal Hortikultura Indonesia (JHI) Vol. 15 No. 3 (2024): Jurnal Hortikultura Indonesia (JHI)
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.15.3.133-139

Abstract

Bunga bangkai raksasa (Amorphophallus titanum (Becc.) Becc. merupakan jenis tumbuhan yang dilindungi. Regenerasi biji di habitat alami sulit dan memerlukan waktu yang lama. Konservasi ex situ dengan kultur in vitro menjadi alternatif pemulihan populasi di habitat alaminya. Tujuan penelitian ini adalah mengetahui jenis eksplan dan zat pengatur tumbuh terbaik untuk menginduksi kalus A. titanum. Jenis eksplan yang digunakan antara lain petiolus, percabangan lamina daun dan lamina daun, sedangkan zat pengatur tumbuh yang digunakan antara lain BAP, CPPU, dan TDZ. Hasil penelitian menunjukkan petiolus yang dikultur secara vertikal mampu menghasilkan kalus lebih banyak dan waktu yang lebih pendek dibandingkan yang dikultur secara horisontal. Eksplan petiolus yang dikultur secara vertikal merupakan eksplan yang menghasilkan kalus terbanyak dan waktu inisiasi tercepat dibandingkan eksplan percabangan lamina daun dan lamina daun. Media MS dengan penambahan TDZ 1 mg L-1 merupakan media terbaik dalam menghasilkan kalus. Waktu inisiasi kalus tercepat diperoleh pada media MS dengan penambahan TDZ 1 mg L-1 dengan eksplan petiolus yang dikultur secara vertikal. Kata kunci: Amorphophallus titanum, petiolus, thidiazuron, zat pengatur tumbuh
ENTREPRENEURSHIP OF HEALTHY VEGETABLE BY HYDROPONIC METHOD TO EMPOWER KARANG TARUNA COMMUNITY: WIRAUSAHA SAYUR SEHAT BEBAS PESTISIDA DENGAN METODE HIDROPONIK UNTUK MEMBERDAYAKAN KOMUNITAS KARANG TARUNA Sri Wulan Manuhara, Yosephine; Winarni, Dwi; Sugiharto; Sugiarso, R. Djarot
Darmabakti Cendekia: Journal of Community Service and Engagements Vol. 6 No. 1 (2024): JUNE 2024
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/dc.V6.I1.2024.84-90

Abstract

Background: Banyuwangi Regency is a popular tourist location in East Java Province. However, after the beginning of the Covid-19 epidemic in early 2020, visitor visitation have significantly declined. This causes a decline in people's economic activity. One of the affected communities was Kemiri in the Singojuruh sub-district, where many young people lost their jobs. Objective: To empower the Karang Taruna youth community in Kemiri village to be entrepreneurs by planting a variety of vegetables such as pakcoi, lettuce, red spinach, and others using the NFT (nutrient film technique) hydroponic system method on unproductive land. Method: This community service activity is divided into five activities, namely training on vegetable plant cultivation using NFT hydroponic system techniques, making green house, making hydroponic installation, planting and maintaining of packcoi and red spinach, harvesting and packaging. Results: After training in basic hydroponic techniques, how to seed seeds and making AB mix nutrients, Karang Taruna youth can practice growing pakcoi and red spinach hydroponically in the green house. At the age of four weeks, after the seedlings are transferred into the hydroponic system, pakcoi vegetables and red spinach can be harvested and ready for sale. Success indicators are measured using a pre-test and post-test to determine mitra's knowledge regarding hydroponic cultivation techniques. Conclusion: Mitra understanding and practicing hydroponic vegetable cultivation techniques. In addition, the youth of Karang Taruna Desa Kemiri, Kab. Banyuwangi, can supplement their income by selling produced veggies.
Co-Authors Abdul Hakim Muhsyi Ahimsa Buena Merpati Alfiah Hayati Alfinda Novi Kristanti Anjar Tri Wibowo Arga Wal Yudha Arif Yachya Boon Chin Tan Boon Chin Tan Dannis Yuda Kusuma Dannis Yuda Kusuma Dannis Yuda Kusuma Dewi, Firli Rahma Primula Djarot Sugiarso Djarot Sugiarso Dwi Winarni Hari Soepriandono Harmami Harmami Hendro Juwono Idqa Nurtri Bhakti Ira Nailas Sa'adah Ita Ulfin Jayanti, Nur Afni Junairiah Junairiah Kusuma, Dannis Yuda Kusuma, Dannis Yuda Lilis Sulistyorini Manikya Pramudya Muhammad Hilman Fu'adil Amin Nabilatun Nisa Ni'mah, Yatim Lailun Ni'matuzahroh Ni'matuzahroh Nindi Novia Erin Ni’matuzzahroh Ni’matuzahroh Nur Syamsiatul Fajar, Nur Syamsiatul Nurul Sofi Amalia Oktavia, Gebby Agnessya Esa Palupi Dasawulan Lestari Putri Gehasti Putri, Angelia Krisdanti Nasera R. Djarot Sugiarso Rachmah, Artifa Rafika Lailiyatul Kurnia Sari Rafika Lailiyatul Kurnia Sari S. Sugiharto Sofiana, Dewi Amelia Sugiarso, R. Djarot Sugiharto Sugiharto Surahmaida, Surahmaida Syifa Fajrisani Ufairanisa Islamatasya Ufairanisa Islamatasya Wijayanti, Ely Tri Yohanes Kartjito Putro