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All Journal HAYATI Journal of Biosciences Jurnal Hortikultura Indonesia (JHI) BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology Jurnal Kimia Riset Caraka Tani: Journal of Sustainable Agriculture Biosaintifika: Journal of Biology & Biology Education PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Darmabakti Cendekia: Journal of Community Service and Engagements Journal Pharmasci (Journal of Pharmacy and Science) Sewagati Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Yosephine Sri Wulan Manuhara
Department Of Biology, Faculty Of Science And Technology, Airlangga University, Surabaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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OPTIMIZATION OF Talinum paniculatum Gaertn. ROOT INDUCTION AND THE EFFECT OF PHOSPHATE CONCENTRATIONS AND AMMONIUM:NITRATE RATIO ON BIOMASS OF ADVENTITIOUS ROOTS IN IN VITRO CULTURE Manuhara, Yosephine Sri Wulan; Palupi Dasawulan Lestari; Syifa Fajrisani; Putri Gehasti; Sugiharto
BIOTROPIA Vol. 30 No. 2 (2023): BIOTROPIA Vol. 30 No. 2 August 2023
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2023.30.2.1678

Abstract

Java ginseng (Talinum paniculatum Gaertn.) is a medicinal plant, the roots of which are commonly used in traditional medicine. In its natural habitat, the roots grow very slowly, requiring two to three years to produce 100 g of roots per plant. Plant tissue culture could therefore provide an alternative means of accelerating root growth. This research aimed to optimize root induction and determine the effect of phosphate (KH2PO4) concentration and the ratio between ammonium and nitrate (ammonium:nitrate) on the biomass of Java ginseng adventitious roots in in vitro culture. Stem and leaf were used as explants and various combinations and concentrations of IBA and BAP, kinetin, and TDZ were used as growth regulators. Leaf explants were grown in Murashige and Skoog (MS) media supplemented with IBA 2 mg/L and various concentrations of phosphate (170; 212.5; 255; 297.5; 340; 382.5; 425; 467.5; 510 mg/L) and various ammonium:nitrate ratios (21:19 mM as the control, 0:30 mM, 10:20 mM, 15:15 mM, 20:10 mM, 30:0 mM). Cultures were maintained for 6 weeks. The observed parameters were fresh weight, dry weight, the duration of root formation, and the number and length of adventitious roots. The data were analyzed using Analysis of Variance. The results showed that the concentration of phosphate and the ammonium:nitrate ratio significantly influenced the amount, length, fresh weight, and dry weight of Java ginseng adventitious root. The highest fresh weight (37.47 mg) and dry weight (5.53 mg) were achieved in the treatment of double phosphate concentration (KH2PO4 340 mg/L), while an ammonium:nitrate ratio of 10:20 mM was the optimum treatment to produce the highest biomass (fresh weight 73.6 mg and dry weight 8.2 mg).
Peningkatan Kompetensi Guru Biologi SMA/MA Kabupaten Bojonegoro Melalui Pelatihan Genetika Molekuler: Improving the Competence of Biology Teachers at SMA/MA in Bojonegoro Regency Through Molecular Genetics Training Sugiharto, Sugiharto; Amin, Muhammad Hilman Fu'adil; Pramudya, Manikya; Hayati, Alfiah; Dewi, Firli Rahma Primula; Manuhara, Yosephine Sri Wulan; Soepriandono, Hari
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 11 No. Suppl-1 (2026): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v11iSuppl-1.11116

Abstract

Molecular Genetics has a positive impact on life. The SMA/MA curriculum has included this material as a mandatory subject for grade 12th. There are obstacles to its implementation due to teachers' lack of understanding and skills. The purpose of the community service program is to improve the competence of SMA/MA teachers in the molecular field, especially those who are members of MGMP Bojonegoro Regency. The activity was conducted on Saturday, August 30, 2025, at the Molecular Genetics Laboratory of the Biology Department, Airlangga University. A total of 25 participants attended this activity, which was coordinated by the Chair and Secretary of MGMP. Participants were given lectures and practicums on the technique of using micropipettes; isolating DNA and measuring its purity; and principles and materials for DNA amplification with PCR and electrophoresis. Referring to program results (1) an increase in highest score range during post-test (range 61-80 = 44%) compared to pre-test (range 21-40 = 60%), (2) highest score achieved by participants (pre-test = 70 is 4%, post-test = 90 is 20%) (3) percentage of increase in participants scores of more than 100% is 52%, (4) participants gave scores on satisfaction index above 95%, it can be concluded that this program can improve competence of Biology teachers at SMA/MA Bojonegoro Regency. This competency improvement is an effort to support the learning process and enhance the quality of education at the SMA/MA level, enabling students to excel in science.
Optimization of Plant Growth Regulators and Bioreactor Systems for Efficient In Vitro Shoot Multiplication and Elongation of Amorphophallus muelleri Blume Jayanti, Nur Afni; Putri, Angelia Krisdanti Nasera; Manuhara, Yosephine Sri Wulan; Wibowo, Anjar Tri
Caraka Tani: Journal of Sustainable Agriculture Vol 40, No 4 (2025): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v40i4.103957

Abstract

Porang (Amorphophallus muelleri Blume), an Indonesian endemic species rich in glucomannan, is widely utilized in food industries for its health benefits. However, its traditional vegetative propagation through bulbils produces limited plants, necessitating an efficient in vitro multiplication system. This study aimed to optimize plant growth regulator combinations and bioreactor systems for enhanced shoot induction and elongation of A. muelleri. During the initiation and induction stages, combinations of thidiazuron (TDZ) with benzylaminopurine (BAP) or kinetin at concentrations ranging from 0 to 1.00 mg l⁻¹ were applied to solid MS media and observed for 8 weeks. The combination of TDZ 0.50 mg l⁻¹ + BAP 1.00 mg l⁻¹ produced the fastest shoot induction (32.34±2.52 days), while TDZ 0.75 mg l⁻¹ + BAP 0.75 mg l⁻¹ resulted in the highest shoot number (76.33±22.81 shoots explant⁻¹) and 100% shoot formation. TDZ combined with kinetin had no significant effect on induction parameters. For elongation, shoots induced from TDZ 0.75 mg l⁻¹ + BAP 0.75 mg l⁻¹ medium were cultured in two bioreactor systems: a Temporary Immersion System (TIS) and a Balloon Type Bubble Bioreactor (BTBB). The BTBB significantly outperformed the TIS, yielding 85.2±3.35 shoots explant⁻¹ with an average shoot length of 5.67±1.13 mm after 4 weeks. These results indicate that the synergistic use of TDZ and BAP, combined with the BTBB system, substantially improves in vitro shoot multiplication and elongation of porang, providing a reliable approach for its large-scale propagation and conservation.
Co-Authors Abdul Hakim Muhsyi Ahimsa Buena Merpati Alfiah Hayati Alfinda Novi Kristanti Anjar Tri Wibowo Arga Wal Yudha Arif Yachya Boon Chin Tan Boon Chin Tan Dannis Yuda Kusuma Dannis Yuda Kusuma Dannis Yuda Kusuma Dewi, Firli Rahma Primula Djarot Sugiarso Djarot Sugiarso Dwi Winarni Hari Soepriandono Harmami Harmami Hendro Juwono Idqa Nurtri Bhakti Ira Nailas Sa'adah Ita Ulfin Jayanti, Nur Afni Junairiah Junairiah Kusuma, Dannis Yuda Kusuma, Dannis Yuda Lilis Sulistyorini Manikya Pramudya Muhammad Hilman Fu'adil Amin Nabilatun Nisa Ni'mah, Yatim Lailun Ni'matuzahroh Ni'matuzahroh Nindi Novia Erin Ni’matuzzahroh Ni’matuzahroh Nur Syamsiatul Fajar, Nur Syamsiatul Nurul Sofi Amalia Oktavia, Gebby Agnessya Esa Palupi Dasawulan Lestari Putri Gehasti Putri, Angelia Krisdanti Nasera R. Djarot Sugiarso Rachmah, Artifa Rafika Lailiyatul Kurnia Sari Rafika Lailiyatul Kurnia Sari S. Sugiharto Sofiana, Dewi Amelia Sugiarso, R. Djarot Sugiharto Sugiharto Surahmaida, Surahmaida Syifa Fajrisani Ufairanisa Islamatasya Ufairanisa Islamatasya Wijayanti, Ely Tri Yohanes Kartjito Putro