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The effect of administering mangosteen rind extract (garnicia mangostana l) compared with glimepiride to the blood sugar levels of white male rat (rattus norwegicus l) induced by streptozotocin As'ari, Hasyim; Mahartini, Dewi Mutmainnah
Folia Medica Indonesiana Vol. 52, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

The purpose of this study is to prove the differential effect of administering the mangosteen Rind extract due to lowering the blood sugar levels of Winstar white male rats induced by streptozotosin (STZ). This study used a randomized design of the ramdomized posttest only control group design. The sample consisted of 4 groups with a sample size of 7 animals each group of rats. All samples were prepared STZ induction of diabetes with a single dose of 50 mg/Kg BW through intraperitoneal (IP). K0 (control group; given 1% CMC 1 cc/day), K1 (treatment group 1; given 0,054 mg glimepiride/200 grBW, K2 (treatment group 2; given mangosteen Rind extract 50 mg/Kg BW), K3 (treatment group 3; given the mangosteen Rind extract 100 mg/Kg BW). Timing of therapy in each group was for 7 days. Shapiro-Wilk normality test (a=0.05) in the BW D variable data, pre-post STZ GDP and D GDP. Testing homogeneity used Levene's test. When data distribution was normal and homogenous, it used an ANOVA deferential test. When data distribution was normal and inhomogeneous, it proceed to apply T-test with 2 free samples. The results: 1) There were significant differences between K0 D GDP with K1 (p=0.015), K0 to K2 (p=0.003) and group K0 to K3 (p=0.002), 2) Whereas no difference was shown in the K1 with K2 (p=0.442), K1 to K3 (p=0,401) and K2 to K3 (p=0.878). Conclusion: The administration of mangosteen Rind extract doses of 50 and 100 mg/kg bw/day did not differ in lowering blood sugar levels compared with glimepiride administration of a dose of 0.054 mg/200 grBW rat/day. Mangosteen Rind extract dose of 100 mg/kg bw/day and 50 mg/kg bw/day did not provide a significant difference in lowering blood sugar levels.
Decrease of LDL Cholesterol Through The Increase of HDL Cholesterol by Administering Garcinia mangostana L. Peel Extract in White Mice As'ari, Hasyim
Folia Medica Indonesiana Vol. 57, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Highlight: • Ethanol extract of Garcinia mangostana L. peel reduce malondialdehyde. • Garcinia mangostana L. peel extract can increase non-radical products which impact in decreasing LDL cholesterol and increasing HDL cholesterol. Abstract: Atherosclerosis contributes to coronary heart disease which may lead to fatality. High cholesterol consumption, stress, and smoking can increase LDL cholesterol in the blood. Consumption of unsaturated fats, high fiber foods, exercise, quitting smoking, losing weight, and giving hypolipidemic drugs, especially herbs, can increase HDL cholesterol and decrease LDL cholesterol. Garcinia mangostana L. peel extract can decrease LDL cholesterol by increasing reverse HDL cholesterol transport to the liver. The study used post test control group design. This study was experimental laboratory research with population of hypercholesterolemic male white mice aged 3-4 weeks with 100-200 grams weight. The HDL and LDL cholesterol data were collected through an enzymatic method by spectrophotometer. This study used analysis of variance (Anova) with significance level of α <0.05. The experiment divided the subjects into positive and negative control groups with dosage variations of 50, 150, 250, and 350 mg/kgBW. Examination of hypercholesterolemia in white mice was conducted on the 8th day. The examination of HDL and LDL cholesterol given peel extract of Garcinia mangostana L. was conducted on the 22nd day. The analysis showed that giving Garcinia mangostana L. peel extract for various dosages could significantly decrease LDL cholesterol and increase HDL cholesterol (p <0.05). Peel extract of Garcinia mangostana L. that contained mangosteen could increase non-radical products that could prevent the transfer of ester cholesterol from HDL to VLDL which impact in increasing HDL cholesterol and decreasing LDL cholesterol.
Isolasi dan Uji Antagonistik Bakteri Endofit Akar Tanaman Padi Terhadap Pertumbuhan Fungi Pyricularia oryzae Penyebab Penyakit Blas Dagsy, Irqami Rachma Dwi; As'ari, Hasyim
Biosfer : Jurnal Biologi dan Pendidikan Biologi Vol. 10 No. 2 (2025): BIOSFER: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP Unpas,

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23969/biosfer.v10i2.32984

Abstract

Penyakit blas yang disebabkan oleh Pyricularia oryzae merupakan salah satu masalah utama pada produksi padi di Indonesia, dengan potensi kerugian hasil mencapai 90% pada varietas rentan. Pengendalian penyakit blas masih bergantung pada varietas tahan dan penggunaan fungisida kimia. Penggunaan fungisida kimia memiliki dampak negatif terhadap pencemaran lingkungan, sehingga diperlukan penangan secara hayati dengan memanfaat bakteri endofit yang mempunyai kemampuan sebagai agen biocontrol.  Penelitian yang dilakuakn bertujuan untuk mengisolasi dan mengetahui aktivitas biokontrol berbagai bakteri endofid  akar tanaman padi terhadap fungi P. oryzae penyebab penyakit blas. Metode penelitian meliputi isolasi bakteri endofit dari akar padi varietas IR-46, dan dilakukan karakterisasi secara makroskopik, mikroskopik, uji biokimia, serta pengujian aktivitas biologis (produksi IAA dan pelarutan fosfat). Uji antagonistik bakteri endofit akar padi dilakukan dengan menggunakan metode kultivasi bersamaan pada media PDA serta mengukur zona hambat terbentuk di sekitar koloni bakteri dan fungi uji, yang dilakukan pengulangan 3 kali. Hasil isolasi dan karaterisasi ditemukan 3 tiga isolat yaitu dari genus Microbacterium, Enterobacter, dan Stenotrophomonas. Hasil  uji antagonis dari ke tiga isolat bakteri endofit akar padi yang di isolasi didapatkan zona hambat terbesar pada genus Stenotrophomonas (10,6 ± 1,05 mm), diikuti Microbacterium (7,13 ± 0,75 mm), dan Enterobacter (4,87 ± 0,76 mm). Kesimpulan penelitian adalah isolat Stenotrophomonas memiliki potensi sebagai agen biokontrol penyakit blas pada padi, dan berpotensi sebagai strategi pengendalian hayati ramah lingkungan dalam mendukung sistem pertanian organik berkelanjutan
Exploration of Industry Partner Support in the Implementation of Internships in the Independent Learning–Independent Campus Program Fatah, Amir; As'ari, Hasyim
Edutechnium Journal of Educational Technology Vol. 3 No. 2 (2025): Edutechnium Journal of Educational Technology
Publisher : CV. Edutechnium Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71365/edujet.v3i2.55

Abstract

The independent learning campus policy (MBKM) by the Minister of Education and Culture of Indonesia has given a new nuance in implementing education in higher education. Students through MBKM are given the right to get hands-on experience in the field for a minimum of three semesters through eight activity programs, one of which is an internship in industry. Industrial internships are learning activities off campus or in the field that involve students, universities, and industry. Partner industries strongly influence the success of this internship program, as industrial internships in the MBKM program require students to study in partner industries for six months. The purpose of this study is to describe the support of industrial partners in the implementation of industrial internships and to describe the forms of industrial support in realizing student independence in learning. This research is a qualitative descriptive study. The research was conducted in 39 partner industries in the industrial internship program for students of the Automotive Engineering Faculty of Engineering, Yogyakarta State University, in 2020, spread throughout Indonesia. Data collection techniques are forum discussion groups (FGD) and documentation. The results show that at the MBKM internship planning stage, partner industries have been involved in mapping the number of students who can participate in the program, offering models of internship forms according to partner industry conditions, and evaluation models that allow optimizing the implementation of internships. At the internship implementation stage, the partner industry supports students conducting industrial practices in their field of interest. It will enable participants to analyze partner industry deficiencies, find solutions, and apply them directly to the field.