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Amri, Indah Amalia
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Journal : Veterinary Biomedical and Clinical Journal

 1 
Identification of Colibacillosis and Antibiotic Resistance Test in Free-range Chicken: Identifikasi Kolibasilosis dan Uji Resistensi Antibiotik pada Ayam Kampung Wulandari, Eka; Amri, Indah Amalia
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.4

Abstract

Escherichia coli are normal flora in the digestive tract of animals and humans but can be opportunistic under certain conditions. Apart from normal flora, there are also pathogenic strains of the E. coli bacteria that can cause disease in the hospes. The disease that can be caused by E. coli bacteria is colibacillosis which often occurs in poultry. One of the clinical symptoms that appear in chickens suffering from colibasillosis is diarrhea which can arise due to enteritis. This study aims to identify the incidence of colibacillosis and antibiotic resistance of E. coli bacteria in Free-range Chicken. Clinical symptoms showed that the animal looked weak, had diarrhea, and had feces around the cloaca. The necropsy results showed the presence of hemorrhage in the duodenum and caecum. Samples of organ swabs that experienced hemorrhage were then tested for isolation and identification of E.coli bacteria and antibiotic resistance using the Kirby-Bauer method for five types of antibiotics, penicillin, bacitracin, gentamicin, cefadroxil, and ciprofloxacin. The test results showed that the Free-range Chicken had colibacillosis. The E. coli bacteria that cause colibacillosis were resistant to penicillin and bacitracin antibiotics, intermediates to cefadroxil, and sensitive to ciprofloxacin and gentamicin.
Effect of Oregano (Origanum vulgare) Extract on Kidney Histomorphometry and Histopathology in Mice (Mus musculus) Colibacillosis Model: Pengaruh Pemberian Ekstrak Oregano (Origanum vulgare) terhadap Histomorfometri dan Histopatologi Ginjal pada Mencit (Mus musculus) Model Kolibasilosis Alviano, Bian Febry Rohmana; Amri, Indah Amalia
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.4

Abstract

Colibacillosis is a disease that infects the digestive tract due to infection with the pathogen Escherichia coli. E. coli is able to cause bacteremia so that E. coli can reach the kidney through blood flowing to the kidney. To reduce the side effects of treatment using antibiotics, the herbal medicine oregano (Origanum vulgare) which contains phenols (carvacrol and thymol) works as an antibacterial. The benefits of this research are as a source of information and the basis for further research related to the use of oregano extract in the treatment of colibacillosis due to E. coli infection. The study used 20 male mice, divided into 5 applicable groups, namely K-, healthy mice were not given any treatment; K+ was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml; P1 was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml + Origanum vulgare extract 25 mg/kg; P2 was induced by Streptomycin 20 mg and infected with Escherichia coli 3 x 109 CFU/ml, 0.5ml + Origanum vulgare extract 50mg/kg; P3 was induced by Streptomycin 20 mg and infected with E.coli 3 x 109 CFU/ml, 0.5 ml + Origanum vulgare extract 100 mg/kg. Kidney was taken for histopathological preparations with hematoxylin eosin (HE) staining. After that, it was analyzed using Kruskal Wallis and continued with Mann Whitney. Histomorphometry semiquantitative measures the width of the Bowman's glomerular capsule and the length of the tubular lumen and then analyzed using One Way Analysis of Variance (ANOVA). The results of this study showed that oregano extract had an effect on histomorphometry and histopathology of renal, with an optimal dose is 5 mg/mice.
Antibiotic Sensitivity Profile of Pasteurella multocida in Cattle in Malang, Indonesia Dodik Prasetyo; Amri, Indah Amalia; Dian Siswanto; Rahayu Shafa Camila Sanjoyo; Monica Widyaningrum; Tanti Widya; Fidi Nur Eka Puji Dameanti
Veterinary Biomedical and Clinical Journal Vol. 6 No. 2 (2024):
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.02.4

Abstract

This study was conducted in Malang, Indonesia, to assess the prevalence and antibiotic susceptibility of Pasteurella multocida in cattle exhibiting clinical signs of respiratory disease. Nasal and tracheal swabs were collected from 50 cattle across multiple farms and tested for P. multocida. The isolates were identified through colony morphology, Gram staining, and biochemical tests, with 4 out of 50 samples (8%) testing positive for P. multocida. Antibiotic susceptibility was determined using the disk diffusion method, involving seven antibiotics commonly used in veterinary practice. Results showed that 75% of the isolates were sensitive to ceftriaxone, while amoxicillin-clavulanic acid was effective in 50% of cases. However, all isolates demonstrated resistance to erythromycin and tetracycline. These findings emphasize the importance of susceptibility testing to guide appropriate antibiotic therapy and prevent the development of resistance. Further research is necessary to monitor resistance patterns and improve treatment strategies for pasteurellosis in cattle.
Co-Authors Adrenalin, Sruti Listra Aeka, Ajeng Agustin, Yuliana Akramsyah, Muhammad Ali Albiruni Haryo, Albiruni Alviano, Bian Febry Rohmana Anggaini, Diva Septia Anisa, Agri Kaltaria Anna Roosdiana Assifa Mardiyah, Fraya Auranzha, Avriell Jasmine Cahyanissa, Ardilla Rachma Dameanti, Fidi Nur Aini Eka Puji Dian Siswanto Dodik Prasetyo Eka Wulandari Fahmiantika, Reza Fajar Shodiq Permata Fidi Nur Eka Puji Dameanti Geoputri, Kartika Myrnandari Hardian, Andreas Bandang Hendrasmara, Muhamad Ferian Izofani, Safira Kamulyan, Ujiati Kurniawan, Septian Bestart Lesmana, Muhammad Arfan Luthfiana, Nurul Monica Widyaningrum Mustofa Helmi Effendi Noviatri, Aldila Orlona, Vanessa Prudence Paramanandi, Dini Agusti Pratama, Alfaro Rikko Purwatiningsih, Wawid Qosimah, Dahliatul Rahayu Shafa Camila Sanjoyo Residiwati, Gretania Rickyawan, Nofan Sarapil, Aurelia Feodora Quon Siti Kurniawati Sutrisno, Rahayu Syah, Ali Akram Tanti Widya Tedja, Dhaneswara Tuska, Habib Syaiful Arif Viski Fitri Hendrawan Winda Syafitri, Winda Wisesa, Ida Bagus Gde Rama Yiondra, Putri Archangela lzzah Yudit Oktanella, Yudit