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All Journal Biomedical Journal of Indonesia Jurnal Penelitian Kesehatan Suara Forikes THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Kesehatan Vokasional Klinikal Sains (Jurnal Analis Kesehatan) Sainmatika: Jurnal Ilmiah Matematika dan Ilmu Pengetahuan Alam Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Jurnal Kesehatan Saelmakers PERDANA (JKSP) 2-TRIK: TUNAS-TUNAS RISET KESEHATAN Jurnal Analis Medika Biosains (JAMBS) Jurnal Riset Kesehatan Poltekkes Depkes Bandung INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Medicra (Journal of Medical Laboratory Science/Technology) Community Empowerment Mitra Raflesia (Journal of Health Science) Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Masker Medika J.Abdimas: Community Health Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Khidmah: Jurnal Pengabdian Kepada Masyarakat JMLS: Journal of Medical Laboratory and Science Hasil Karya Aisyiyah Untuk Indonesia Journal Health Applied Science And Technology (JHAST) Jurnal Inspirasi Kesehatan (JIKA) Jurnal Media Analis Kesehatan
Bastian Bastian
DIV Teknologi Laboratorium Medis IKesT Muhammadiyah Palembang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Perbandingan Jumlah Total Koloni Bakteri Klebsiella Pneumonia Pada Media Nutrient Agar Dan Media Alternatif Bengkuang (Pachyrrhizus Erosus) Bastian Bastian
Mitra Raflesia (Journal of Health Science) Vol 15, No 1 (2023)
Publisher : LPPM STIKES BHAKTI HUSADA BENGKULU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51712/mitraraflesia.v15i1.158

Abstract

Latar Belakang : Pneumonia adalah penyakit infeksi akut yang menyerang jaringan paru-paru (alveoli), menyebabkan kematian terbesar pada anak di seluruh dunia. Jumlah kasus pneumonia balita di Provinsi Sumatera Selatan menempati posisi ke-19 sebesar 37, 36%. Salah satu bakteri penyebab pneumonia adalah Klebsiella pneumonia. Diagnostik laboratorium pada bakteri Klebsiella pneumoniae yaitu dapat dilakukan dengan cara isolasi bakteri, pewarnaan gram, dan uji biokimia. Bakteri Klebsiella pneumonia membutuhkan sumber nutrisi terutama karbohidrat untuk tumbuh dan berkembang-biak. Gold standar diagnostik yang digunakan dalam pemeriksaan adalah kultur pada media. Isolasi dapat menggunakan media sintetis dan media alami seperti bengkuang karena memiliki kandungan karbohidrat jenis inulin (Oligosakarida) sebanyak 7,2 gr, vitamin, nutrisi dan lainnya yang dibutuhkan untuk pertumbuhan bakteri. Metode : Jenis penelitian eksperimen murni (true experiment). Penelitian ini dilakukan di laboratorium mikrobiologi Institut Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang pada tanggal 10 januari 2022 - 14 januari 2022 dengan jumlah pengulangan sebanyak 32 pengulanganHasil : Nilai rata-rata jumlah total koloni bakteri Klebsiella pneumonia pada media Nutrient Agar (NA) 198 CFU/mL, dan media alternatif bengkuang (Pachyrrhizus erosus) 234 CFU/mL. Uji T tidak berpasangan didapatkan nilai p (Sig 2 Tailed) = 0,029.Simpulan : Hasil penelitian yaitu bengkuang (Pachyrrhizur erosus) dapat digunakan sebagai media alternatif pertumbuhan bakteri Klebsiella pneumoniaKata Kunci : Klebsiella pneumonia, Nutrient Agar dan bengkuang 
KOLERASI JUMLAH LEUKOSIT TERHADAP KADAR CRP PADA PENDERITA PENYAKIT TUBERKULOSIS Bastian Darwin; Meyrita Birka Yolanda
jurnal kesehatan terapan sains dan teknologi Vol 1 No 1 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i1.1

Abstract

Introduction: Tuberculosis is one of the most common infectious diseases in the community with a percentage of 80% caused by Acid Resistant Bacillus (BTA), namely the bacterium Mycobacterium tuberculosis. Leukocytes act as an immune system or kill germs and diseases that are in the human bloodstream. In addition to leukocytes, there are CRP parameters that help fight tuberculosis infection, namely by releasing various pro-inflammatory cytokines and bacterial invasion occurs which then induces liver cells to synthesize acute phase proteins such as CRP which will increase sharply for a few moments, inflammation will occur during the systemic inflammatory process. Purpose: This study aims to determine the correlation between leukocyte count and CRP levels in tuberculosis patients. Research Method: This research was conducted in the Hematology Laboratory of the Muhammadiyah Palembang Institute of Health Sciences and Technology in March-April 2021. This type of research is Analytical Descriptive research with a cross sectional approach. Data were analyzed using Spearmen's correlation test. Results: This study had an average leukocyte count of 4.85 cells/µL, CRP examination results with an average value of 9.60 mg/L and Spearman's correlation test with p = 0.113. Conclusion: There is no correlation between leukocyte count and CRP levels in tuberculosis patients.
Perbedaan Kadar Hemoglobin Segera Diperiksa dan Penundaan 2,5 Jam Pada Suhu Ruang Indah Sari; Ema Karlina; Bastian
jurnal kesehatan terapan sains dan teknologi Vol 1 No 1 (2023): Journal Health Applied Science And Technology (JHAST)
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jhast.v1i1.5

Abstract

Hemoglobin is a protein molecule in red blood cells that functions as a means of transporting oxygen (O2) from the lungs to tissues throughout the body and taking carbon dioxide (CO2) from the tissue below to the lungs to be released into the free air. This study aims to determine the effect between blood immediately examined and a delay of 2.5 hours at room temperature on hemoglobin levels conducted at IKesT Muhammadiyah Palembang. The type of research used is cross sectional with intact group comparison research design. The sample consisted of 30 samples immediately examined and 30 samples with a delay of 2.5 hours taken from 30 respondents. The study was conducted starting from patient preparation, taking examination materials, processing examination materials, analysis and research results. The average hemoglobin level immediately examined was 12.8 g/dL while the hemoglobin level with a delay of 2.5 hours was 12.7 g / dL, the results of the wilxocon test showed that the significant value was p=0.342. The p value obtained is p>0.05. The results can be concluded that there is no difference in hemoglobin levels immediately examined and a delay of 2.5 hours at room temperature.
PERBANDINGAN KADAR ENZIM ALKALINE PHOSPHATASE (ALP) PADA SERUM HEMOLISIS RINGAN DAN NON HEMOLISIS Tria Agustina; Bastian Bastian; Dewi Hartati
Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health) Vol 7 No 1 (2023): Jurnal Bahana Kesehatan Masyarakat (Bahana of Journal Public Health)
Publisher : Poltekkes Kemenkes Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35910/jbkm.v7i1.619

Abstract

Background: Alkaline Phosphatase (ALP) is a hydrolase enzyme that is first produced by the liver epithelium and osteoblasts (cells that form new bone), this enzyme is mostly found in the liver and bones. ALP enzyme examination can be affected if there is damage to the cells, causing haemolysis. Haemolysis can be affected by phlebotomy techniques at an inappropriate pre-analytical stage, so that haemolysis can cause increased concentrations in red blood cells compared to serum or plasma, thus giving false concentration results. This study aims to determine the differences in ALP enzyme levels in mild haemolysed serum and non-haemolysed serum. Methods: The study population included all 17 male students of DIV Medical Technology Laboratory, Faculty of Science and Technology Muhammadiyah Palembang, by differentiating inclusion, namely aged 17 to 22 years, male sex and willing to be respondents. Samples in the form of mild hemolysis and non-hemolysis serum were taken from the respondents. The stages of the research began with taking venous blood samples, processing the blood into non-hemolytic serum and mild hemolytic serum, and ALP examination using the Biosystem BA 200 tool. Results: This study showed that the difference between ALP enzyme levels in non-haemolytic and haemolytic samples was 73.9 U/L or 55%. The data analysis test used the Paired T test which obtained p ≥ 0,000. Conclusion: There are differences in results between mild haemolysed serum and non-haemolysed serum.
Edukasi Pengaruh Pemasangan Tourniquet Terhadap Kadar Elektrolit Pada Petugas Laboratorium Rumah Sakit Musi Medika Cendikia Bastian Bastian
Khidmah Vol 5 No 1 (2023): Khidmah
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/khidmah.v5i1.446

Abstract

Laboratorium merupakan tempat pelaksaan kegiatan pemeriksaan spesimen dari manusia, mengeluarkan hasil penyakit, serta faktor-faktor dari penyakit tersebut. Tahapan pemeriksaan laboratorium ada tiga tahapan yaitu tahap pra analitik, tahap analitik, dan pasca analitik. Tahap pra analitik merupakan tahapan yang paling sering menyumbang kesalahan. Dalam tahap pra analitik salah satunya ada pengambilan spesimen seperti darah. Untuk memudahkan flebotomis mengambil darah maka perlu alat untuk pembendungan darah tersebut yaitu tourniquet. Pemasangan tourniquet tidak diperbolehkan melebih satu menit karena jika lebih akan mempengaruhi hasil pemeriksaan darah tersebut. Salah satu pemeriksaan yang terdampak adalah pemeriksaan elektrolit. Elektrolit merupakan komponen penting dalam tubuh dan jika penghitungan kadarnya salah maka dapat berakibat fatal bagi pasien. Permasalahan yang dihadapi petugas laboratorium RS MMC Palembang adalah kurangnya pemahaman mengenai pemasangan tourniquet dalam pengambilan darah untuk pemeriksaan kadar elektrolit. Sebagai upaya dalam menjawab permasalahan yang dihadapi petugas laboratorium RS MMC Palembang berkaitan dengan hal ini maka perlu dilakukan sosialisasi dan edukasi dengan tujuan dapat meningkatkan pengetahuan dan pemahaman pada petugas laboratorium RS MMC Palembang mengenai pemasangan tourniquet dalam pengambilan darah untuk pemeriksaan elektrolit. Media yang digunakan mengenai pengaruh penggunaan tourniquet terhadap pemeriksaan elektrolit adalah sosialisasi dan edukasi melalui media poster, dan mendemonstrasikannya di akhir kegiatan.
Differences In HbA1c Levels In Diabetes Mellitus Patients Using Capillary And Venous blood Samples Aristoteles; bastian; Teni Lestari
Jurnal Inspirasi Kesehatan Vol. 1 No. 2 (2023): Spesial Edisi Khusus (Proceeding)
Publisher : Fakultas Kesehatan IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/jika.v1i2.52

Abstract

Introduction: HbA1c is a non-enzymatic binding of glucose molecules to hemoglobin through a post- translational glycation process, measuring the level or percentage of glucose bound to hemoglobin, this parameter is used as the main benchmark for controlling Diabetes Mellitus because HbA1c can describe blood sugar levels within a period of 1- 3 months due to red blood cells bound by glucose molecules. HbA1c examination is an examination of glycemic levels which is useful to determine long-term glycemic control in patients with Diabetes Mellitus. From this study to determine differences in HbA1c levels in diabetes mellitus patients using capillary and venous blood samples. Method: Cross-sectional, this research was conducted at the Hematology Laboratory of the Palembang Muhammadiyah Institute of Health and Technology. The population taken was Diabetes Mellitus Patients in the Muhammadiyah Palembang Hospital laboratory. The sample used in this study was 29 samples using purposive sampling. Results: The average HbA1c level was found in 7.91% capillary blood and 7.91% venous blood. Discussion: The conclusion in this study is that capillary and venous blood sampling can be used to check HbA1c levels.
ANALISA KUALITAS PREPARAT TELUR CACING STH MENGGUNAKAN PEWARNAAN EOSIN 2% DAN PEWARNAAN ALTERNATIF LIMBAH GAMBIR Bastian Darwin
Masker Medika Vol 12 No 1 (2024): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v12i1.587

Abstract

Introduction: Worm infections are widespread in tropical and subtropical areas. Especially Indonesia, one of the countries that has quite high cases of worms, these cases are usually found in areas with high humidity, lack of personal hygiene, and poor environmental conditions. One of the laboratory tests that can be done to diagnose worm disease is a microscopic examination. Microscopic examination using stool samples requires staining which of course contains good dyes as support. This coloring substance is found in gambier waste (Uncaria gambier) in the form of catechin which is able to give a red and yellow color. Thus, gambier waste (Uncaria gambier) can be used as an alternative color to examine STH worm eggs. Research objective: to utilize catechins in gambier waste (Uncaria gambier) as a coloring agent to examine STH worm eggs. This research includes collecting feces samples, collecting gambier waste, processing gambier waste, making preparations using eosin staining and staining gambier waste, observing STH worm eggs microscopically. Research method: Laboratory experiment, carried out at the Parasitology Laboratory, Faculty of Science and Technology, Muhammadiyah Institute of Health Sciences and Technology, Palembang. The sample is feces. Research results: show that STH worm eggs can be stained with both stains, namely Gambier Waste Staining and Eosin Staining. Research conclusion: gambier waste coloring can be used as coloring for STH worm eggs. Staining gambier waste can be used as an alternative coloring to replace eosin dye for staining STH worm eggs
Titer Differences in Rheumatoid Factor Level in The Elderly Using The Glycine Saline Buffer Reagent and 0.9% NaCl Ready to Use Bastian Darwin
Masker Medika Vol 11 No 2 (2023): Masker Medika
Publisher : IKesT Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52523/maskermedika.v11i2.597

Abstract

Introduction: The incidence of Rheumatoid Arthritis (RA) in Indonesia has a case prevalence ranging from 0.1% to 0.3%. Limitations in the supply of Glyceline saline buffer in laboratory examinations, especially in the laboratory for examination of Rheumatoid Factor (RF), is one of the reasons for the need for alternative materials with the same effectiveness to be used in examinations. An alternative in carrying out inspections if commercial reagents are not available so that ready-to-use 0.9% NaCl reagent can be an alternative reagent. The purpose of this study was to determine whether there were differences in the titers of rheumatoid factor levels using glycine saline buffer reagents and ready-touse 0.9% NaCl. Method: The study was conducted at the IKesT MP Clinical Pathology Laboratory. The research design used a posttest only control group design. The population is 43 elderly people at the Harapan Kita Nursing Home. a sample of 30 elderly people with purposive sampling technique. Data were analyzed by Wilcoxon test. Results: The results obtained were p = 0.000 for titer examination of RF levels using Glycine Saline Buffer reagent and ready-to-use 0.9% NaCl reagent. Discussion: There are differences in the titers of rheumatoid factor levels using glycerin saline buffer and ready-to-use NaCl. 0,9%.
Analysis of C-Reactive Protein (CRP) Levels in Venous and Capillary Blood Samples with Immunoturbidimetric Methods: Analisa Kadar C-Reactive Protein (CRP) Pada Sampel Darah Vena Dan Darah Kapiler Dengan Metode Imunoturbidimetri Bastian, Bastian; Sari, Indah; Pratama, Feronica Putri
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 5 No. 1 (2022): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i1.1622

Abstract

Inflammation or inflammation occurs due to bacterial infection, detection of inflammatory infection can be done by laboratory tests. An examination that can help diagnose and detect abnormalities in the body. One of the parameters for examining inflammation markers is C-Reactive Protein (CRP). CRP examination usually uses venous blood because venous blood is more often used in laboratory examinations. The purpose of this study was to determine differences in CRP levels in venous and capillary blood. This study uses a posttest only design research. Samples were taken from DIV Technology Medical Laboratory IkesT Muhammadiyah Palembang students with total sampling technique. The research was carried out at the Hematology Laboratory of IKesT Muhammadiyah Palembang on December 21, 2021. Based on the results of research that has been carried out on the analysis of C_Reactive Protein levels in venous blood and capillary blood samples using the immunoturbidemteri method, the results showed that the average CRP level of venous blood was 9.6 and average the average capillary blood CRP level was 8.33. Thus it can be concluded that there is no significant difference in the examination of CRP levels in venous and capillary blood.
Edukasi Pemantapan Mutu Internal Tahap Pra-Analitik Pada Pemeriksaan Glukosa di Puskesmas Talang Pangeran Ogan Ilir Bastian, Bastian; Ulva, Maria
Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Vol. 3 No. 5 (2023): September 2023 - Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat
Publisher : Indonesian Scientific Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59395/altifani.v3i5.476

Abstract

Pemantapan Mutu Internal adalah kegiatan pencegahan dan pengawasan yang dilaksanakan oleh masing-masing laboratorium secara terus menerus agar tidak terjadi dan mengurangi kejadian penyimpangan sehingga diperoleh hasil pemeriksaan yang tepat. Salah satunya pada laboratorium Patologi Klinik yang paling banyak dilakukan yaitu pemeriksaan glukosa darah. Glukosa darah adalah gula yang terdapat dalam darah yang terbentuk dari karbohidrat dalam makanan dan disimpan sebagai glikogen di hati dan otot rangka. Permasalahan yang dihadapi petugas laboratorium Puskesmas Talang Pangeran Ogan Ilir adalah kurangnya pemahaman mengenai pemantapan mutu internal pada Pemeriksaan Glukosa. Sebagai upaya dalam menjawab permasalahan yang dihadapi petugas Puskemas Talang Pangeran Ogan Ilir, maka perlu dilakukan metode sosialisasi dan edukasi melalui media poster dengan tujuan dapat meningkatkan pengetahuan dan pemahaman pada petugas mengenai pentingnya Pemantapan Mutu Internal Pada Pemeriksaan Glukosa. Sehingga dapat disimpulkan bahwa petugas laboratorium Puskesmas Talang Pangeran Ogan Ilir telah memahami tentang pemantapan mutu internal tahap pra-analitik pada pemeriksaan glukosa darah.
Co-Authors Andini Junytri Anggun Sagita Anisa Nur Afifa Anjas Prayoga Annisa Fitri, Annisa APRIANI, DEVI Aristoteles Aristoteles Aristoteles, Aristoteles Asteria, Viola Ayu Enjellya Prisicilya BASTIAN BASTIAN, BASTIAN Cut Nur’azimah Putri Rico Cyndy Hasendra Damayanti , Allya Denny Juraijin Denny Juraijin Depi Anggraini Desika Mela Amelia Dewi Hartati Dewi Hartati Dewi Hartati Dilla Wulandari Eddy Mart Salim, Eddy Mart Eka Yuniar Ema Karlina Endang Endang Faaizah Ramadhania Ferdi Afriansyah Feronica Putri Pratama Firna Kamilatun Nuha Firna Kamilatun Nuha Hani Ammariah Hartati, Dewi Hartati, Dewi Helnia Sari Helniasari Helniasari Heriyanto, Ian Kurniawan Indah Sari Indah Sari Indah Sari Indah Sari Indah Sari Jusak Nugraha Juwy Trianes Juwy Trianes Juwy Trianes kurniati, nova Marlina, Melli Melli Marlina Meyrita Birka Yolanda Muhammad Irsan Saleh Natalia, Anggie Novi Rianti Nurhidayanti Nurhidayanti Nurhidayanti, Nurhidayanti Nurul Pathia Pratama, Feronica Putri Rahadiyanto, Kemas Ya'Kub Rahmadani, Inoy Rizky Redy Novrian Utama Reni Junika Familianti RR. Ella Evrita Hestiandari Salsabillah, Felisa Tanoehardjo, Francisca Srioetami Tanoehardjo, Francisca Srioetami Teni Lestari Tria Agustina Trianes, Juwy Trimin Kartika Try Era Realita Ulva, Maria Veny Tresia Utari Wulandari, Atika Putri