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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Bios Logos Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology JBIO: jurnal biosains (the journal of biosciences) AL KAUNIYAH Journal of Aquatropica Asia Jurnal Pembelajaran dan Biologi Nukleus Jurnal Pengabdian kepada Masyarakat Nusantara Community Empowerment Bioscientist : Jurnal Ilmiah Biologi Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Science Technology and Management Journal (STMJ) CONSERVA : Jurnal Konservasi Sumber Daya Alam dan Lingkungan
Lingga, Rahmad
Department of Biology, Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : Jurnal Pembelajaran dan Biologi Nukleus

 1 
Identifikasi Bakteri Resisten Cu Dari Sedimen Laut Terdampak Aktivitas Tambang Timah || Identification Of Cu Resistant Bacteria From Tin Mining-Affected Sea Sediment Lingga, Rahmad; Afriyansyah, Budi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 6, No 2: Jurnal Pembelajaran Dan Biologi Nukleus September 2020
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v6i2.1666

Abstract

Aktivitas pertambangan timah di laut memberikan pengaruh buruk terhadap kondisi lingkungan salah satunya adalah cemaran logam berat yang dapat mempengaruhi kehidupan biota perairan. Penelitian ini dilakukan untuk mengisolasi dan menguji resistensi bakteri sedimen laut terdampak aktivitas tambang timah terhadap logam berat Cu. Pengambilan sampel dilakukan pada area penambangan timah di laut sampai ke hamparan lumpur kawasan mangrove terdekat. Isolasi bakteri dilakukan dengan metode cawan sebar dan karakterisasi bakteri meliputi pengamatan bentuk sel, pewarnaan Gram dan uji biokimia. Isolat bakteri selanjutnya diuji resistensinya terhadap logam Cu dengan konsentrasi 10 ppm, 20 ppm, 40 ppm, 80 ppm dan 100 ppm. Hasil penelitian memperlihatkan bahwa isolat bakteri yang berasal dari sedimen laut terdampak aktivitas tambang timah memiliki resistensi terhadap perlakuan logam berat Cu pada berbagai konsentrasi. Isolat B6, B8 dan A10 memperlihatkan resistensi tertinggi terhadap Cu sampai pada konsentrasi 100 ppm.Tin mining activity at sea has various negative influences on the environment. One of them is heavy metal contamination that can affect the life of fisheries biota. This research was conducted to isolate and test the Cu heavy metal resistance of marine sediment bacteria that are affected by tin mining activity. Sediment sampling was carried out in the area of tin mining to the mudflat neared to the mangrove area. Bacterial isolation was carried out by spread plate method and bacterial characterization included cell shape, Gram staining and biochemical tests. Furthermore, bacterial isolates were tested for resistance to metals with concentrations of 10 ppm, 20 ppm, 40 ppm, 80 ppm and 100 ppm. The results showed that bacterial isolates originating from marine sediments affected by mining activity were resistant to Cu heavy metal at various concentrations. Isolates B6, B8 and A10 showed the highest resistance up to the concentration of 100 ppm
Identification of Biofilm-Producing Bacteria From Nangka Island Marine Water in District of Bangka Tengah Robika, Robika; Lingga, Rahmad; Afriyansyah, Budi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 8, No 1: Jurnal Pembelajaran Dan Biologi Nukleus Maret 2022
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v8i1.2492

Abstract

Bacterial community is an important element of an ecosystem both on land and in water. In aquatic ecosystems, bacteria that have the ability to produce biofilms have the advantage of being able to easily attach on a substrate. The main function of biofilm is assisting nutrition absorption from water and facilitating bacteria surviveness from unfavorable environmental conditions. This research was conducted to isolate and identify and test the potential of bacteria to form biofilms in vitro. The methodological steps included measuring the physical-chemical parameters of the waters; isolation by pour plating method; characterization and identification  based on morphological, biochemical and Gram staining; as well as testing the activity of biofilm formation by reading technique using microplate reader. The highest bacterial abundance was found at Station 3 (muddy substrate) which was 3.3x106 cfu/ml. Bacterial isolates that had the highest ability to form biofilms in vitro were Bb3, Bb4 and Bb1 isolates with values of 0.3315, 0.2370) and 0.2131, respectively. Bacteria that have the potential to form biofilms from various substrates in the waters of Nangka Island belong to Alcaligenes and Meniscus genera. From the results of the study, it can be concluded that bacteria from rocky substrates have the ability to produce biofilms better than isolates from other substrates.
Literature Study of Lactic Acid Bacteria in Bangka Belitung: Sources, Methodology, Utilization, And Identification Lingga, Rahmad; Saputri, Rinny; Fitriyani, Fitriyani; Indra, Muhammad Panca
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i1.5196

Abstract

Bangka Belitung has various types of fermented foods, in the production of which lactic acid bacteria are involved. Lactic acid bacteria plays important role in enhancing the nutritional value of food ingredients, extending their shelf life, producing distinct aromas, and preventing contamination from pathogenic bacteria. Additionally, lactic acid bacteria can also act as probiotics for application in livestock or fisheries. This article examined several studies on lactic acid bacteria that were linked to the biological resources of Bangka Belitung. The aspects studied included the sources of lactic acid bacterial isolates, research methods or approaches employed, utilization, and identification of lactic acid bacteria. Most of the research related to lactic acid bacteria has been conducted on fermented foods, primarily 'rusip,' which is a fermented food product made from shrimp or fish. This review expected to provide an overview of the trends and research interest in lactic acid bacteria conducted in Bangka Belitung, while also mapping out aspects that need further exploration in subsequent research
Isolation and Identification of Lactic Acid Bacteria from Channa sp. as Potential Probiotic Bayu, Hendi Hendra; Irwanto, Riko; Dalimunthe, Nurzaidah Putri; Lingga, Rahmad
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i1.3551

Abstract

Lactic acid bacteria (LAB) are a group of bacteria that are naturally present in the digestive tract of vertebrates, including fish such as Channa sp. Lactic acid bacteria are considered the most suitable microbes for use as probiotics. Probiotics can produce antimicrobial metabolites so that can improve the microbial balance in the digestive tract. It is believed that Channa sp. harbors LAB in its digestive tract, which may have probiotic potential and produce antimicrobial metabolites that can inhibit pathogenic bacteria. The aim of this study was to isolate and identify LAB from the intestines of Channa sp. The research involved several steps, including sample preparation, LAB isolation, characterization, and purification of bacterial isolates, biochemical tests, temperature resistance tests, antimicrobial tests, haemolysis tests, lactic acid production tests, and cholesterol-lowering activity tests. Eight LAB isolates with potential probiotic characteristics were isolated and identified as the genus Lactobacillus, which are gram-positive bacteria that produce lactic acid during culture
Co-Authors ACENG RUYANI Adibrata, Sudirman ANDRI KURNIAWAN Arif, Maulana Bayu, Hendi Hendra Budi Afriyansyah Dalimunthe, Nurzaidah Putri Djuria, Rachmawati Felani Edelweis, Mutia Anggita Fauziyah, Zaenab Feni Kurnia, Feni Fitri Afriani Fitriyani Fitriyani Granitio Irwan, Andesta Henny Helmi Henri Henri, Henri Hidayah, Reysya S N Iman Rusmana Indra, Muhammad Panca Insyira, Rania Islamiyah, Umiyatul Julian Erika Putri Juliansyah, Ari Julisa, Shella Indila Lissoliha, Lissoliha Martahayu, Vika Meilya, Risna Meysari, Della Papingka, Tirma Pratoyo, Genta Hazi Priyansah, Sujadi Purba, Eva Dewi Rosmawati Rafsanjani, Rifqi Almusawi Randi Syafutra Rangga, M Yusuf Riko Irwanto Roanisca, Occa Robika, Robika Roshan, Rani Arizki Salmi, Salmi Saputri, Rinny Sembiring, Juhardi Septiani, Hikmah Septiani, Reti Setiadi, Jhodi Sipriyadi Syazili, Ahmad Tiwi, Raka Verry Andre Fabiani Wahyuni, Reza Wibowo, Risky Hadi winanto, winanto Wulandari, Ufi Ayu Yuant Tiandho Yurdayanti, Yurdayanti