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Legowo, Djoko
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Journal : journal of Basic Medical Veterinary

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HISTOPATHOLOGICAL APPEARANCE OF THYMUS ON BROILER UNDER CHRONIC HEAT STRESS Andriani, Ratna; Arimbi, Arimbi; Rahardjo, Dadik; Plumeriastuti, Hani; Legowo, Djoko; Hestianah, Eka Pramyrtha
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19987

Abstract

The aim of this research was to know histopathological change of broiler's thymus which exposed by chronic heat stress. The results revealed that there were negative impact on immune organ (thymus). Broiler that exposed to chronic heat stress showed significantly increased number of heterophil and apoptosis histiocytes and cell depletion. There were 20 broilers that divided into 2 groups, each group consist of 10 broiler. Control group (P0) reared at temperature 24-28oC and humidity 40-55% and Heat Stress group (P1) reared at temperature 36-40oC on the litter housing system at Experimental Animals Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. Before the treatments, broiler had been reared from day-old-chicks to 21-day-old, then continued with heat stress condition from day 22 to 42. Thymus was collected on day 42 to examine histopathological change of thymus and analyzed using Mann-Whitney Test. The results showed significant difference (P<0.05) between Control group and HS (Heat Stress) group by increased number of heterophil, apoptosis histiocytes and cell depletion.
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST HISTOPATHOLOGICAL CHANGES OF SMALL INTESTINE ON MALE WHITE RAT (Rattus norvegicus) WISTAR STRAIN Amaris, Aulia Puspa; Yustinasari, Lita Rakhma; Legowo, Djoko; Plumeriastuti, Hani; Anwar, Chairul; Hidajati, Nove
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20409

Abstract

This study was conducted to investigate the effect of polypropylene residue on heated palm oil against histopathological changes of small intestine on male white rat (Rattus norvegicus) wistar strain. Eighteen male white rats divided into three groups and were administered via peroral gavage with different treatments for five weeks. The treatment were Control (84 grams palm oil), P1 (84 grams palm oil with 2.3 grams plastic), and P2 (84 grams palm oil with 6.81 grams plastic). The histopathological changes of small intestine were examined by using Barthel Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed that were significant difference (p<0.05) between treatment groups. The worse damage of polypropylene plastic residue was 6.81 grams.
HISTOPATHOLOGICAL CHANGES OF KIDNEY OF BROILER CHICKEN EXPOSED TO CHRONIC HEAT STRESS Xin, Irene Teh Kai; Plumeriastuti, Hani; Anwar, Chairul; Rachmawati, Kadek; Utama, Suzanita; Legowo, Djoko
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20411

Abstract

The aim of this research was to know histopathological changes of kidney of broiler chicken exposed to chronic heat stress. Twenty broilers were divided randomly into 2 groups, 10 broilers each are being exposed to chronic heat stress for 21 days. The first 21 days were the adaptation period in a chamber with temperature 24-28o C and humidity 40-55%. After 21 days, continue with exposure to heat stress in a chamber with temperature 36-40o C and humidity 50-65% in 8 hours per day. After exposed to chronic heat stress, kidney tissues were processed, and kidney tissue histopathological changes were evaluated by using the Klopfleisch modified scoring method. The data was analyzed by Mann – Whitney Test. The result of this research showed that chronic heat stress exposure causing the presence of degeneration of tubular epithelial cell, necrosis of tubular epithelial cell, necrosis of glomerular and interstitial infiltration. From the analysis data the overviewof multiparametric showed that when control group compared with the treatment group showed significantly difference (p<0.05). 
KEMAMPUAN MALARISIDAL NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI TERAPI AJUVAN PADA HISTOPATOLOGI HATI MENCIT (Mus musculus) PENDERITA MALARIA Pujiyanto, Mahendra; Maslachah, Lilik; Triakoso, Nusdianto; Lazuardi, Mochamad; Anwar, Chairul; Legowo, Djoko
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21094

Abstract

The aimed of this study is to know malaricidal ability of nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy on liver histopathological of mice (Mus musculus) which infected by Plasmodium berghei. This research uses 30 mice which are divided into six treatment groups namely K0 (not infected without treated), K+ (infected without treated), K1 (infected and treated with chloroquin), K2 (infected and treated with juwet leaf extract), P1 (infected and treated with juwet leaf extract nanoparticle) and P2 (infected and combination treated with chloroquine and juwet leaf extract nanoparticle) which infected by 1x105 in 0.2 ml of Plasmodium berghei. The data were analyzed by Kruskal Wallis using SPSS and followed with Mann-Whitney test. This research result showed there was significant difference (p < 0.05) between K1 and P2, but did not showed significant difference (p > 0.05) with K+, K2 and P1. Based on this research, it could be concluded that nanoparticle juwet (Syzygium cumini) leaf extract as adjuvant therapy estimated can increase malaricidal ability in degeneration, necrosis, portal inflammation, Kupffer cell hyperplasia, sinusoidal congestion, and hemosiderosis in mice liver which infected by Plasmodium berghei.
Identification of Cat (Felis catus) Blood Splatter on Cotton Fabric After Periods of Drying Using Leucomalachite Green and Takayama Reagent Charles, Vanessa Ann; Lestari, Tita Damayanti; Legowo, Djoko; Ismudiono, Ismudiono; Hidajati, Nove; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28593

Abstract

Blood-stain or blood splatter analysis when used properly can assist in establishing a chain of events linked to violent crimes (Bevel and Gardner, 2008). The methods used in detecting blood splatters in the field are chemical methods. Leucomalachite green is a colorimetric test which is used to test the presence of blood (Castro and Coyle, 2008). Takayama reagent is a confirmatory test for blood (Strassman, 1922). The aim of this research is to detect the blood splatter on cotton fabric after it has been dried for 1 day, 3 days and 5 days using Leucomalachite green and Takayama reagent. Cotton fabric was specifically chosen for this experiment with 3 different periods of drying. The unstained cotton fabric was cut into squares, and a blood sample was splattered on each piece. The fabrics splattered with blood were then dried for 1 day, 3 days and 5 days. The blood splatter was then tested using Leucomalachite green and Takayama reagent, and the results were noted afterwards. For the control, red food dye was dried for 1 day then tested with Leucomalachite green and Takayama reagent. The image results of the Leucomalachite green test are analyzed using ImageJ software 1.8.0_112 where the red, green and blue pixels are converted to grayscale. The image results of the Takayama test are graded based on the number and pattern of crystal. In conclusion, Leucomalachite green and Takayama reagent are able to detect cat blood splatter on the cotton fabric. Leucomalachite green produced a higher intensity/ darker colour as a result of an older sample, and the lower intensity/ lighter colour as a result of a fresher sample of the Leucomalachite green test. Takayama reagent produced a densely packed pattern of crystals as a result of an older sample, and the loosely packed pattern of crystals as a result of a fresher sample of the Takayama test.
Acute Toxicity Test Of The Green Viper Snake (Trimeresurus albolabris), Macroscopic Description Of The Kidney And Liver Of Mice (Mus musculus) Darmawan, Moch. Rizky; Rahardjo, Dadik; Tyasningsih, Wiwiek; Kurnijasanti, Rochmah; Legowo, Djoko; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31135

Abstract

According to WHO (2010), the Trimeresurus albolabris snake is a venomous snake with a high number of bite cases in Indonesia. Information on the character and level of toxicity of snake venom in Indonesia, including Trimeresurus spp. snakes, is still minimal. This study aims to determine the LD50 value (lethal dose 50), clinical symptoms, and to determine the macroscopic appearance of the liver and kidneys of mice (Mus musculus) after being injected by a the white-lipped pit viper (Trimeresurus albolabris) venom. The LD50 value of Trimeresurus albolabris venom injected subcutaneously into mice was 5,09 mg/kgBW. Clinical symptoms that appear increased movement activity, increased respiratory rate, lethargy, decreased activity, and reduced grooming. Macroscopic appearance of the liver that looks abnormal is found (1) color changes in the form of pale, pale yellowish, and red spots (petechiae). (2) changes in surface structure found only one liver that is not flat. (3) changes in consistency in the form of brittle easily. In the kidney that looks abnormal, found (1) changes in color in the form of pale. (2) changes in surface structure were only found in one kidney in the form of nodules. (3) changes in consistency in the form of brittle easily and soft.
The Effect of Mimosa Pudica Root Extract on Cerebrum Histopathological of Rattus Norvegicus Induced with Naja Sputatrix Venom Permatasari, Roselia Yuliani; Hestianah, Eka Pramyrtha; Legowo, Djoko; Rachmawati, Kadek; Arifin, Zainal
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36485

Abstract

The aim of this study was to know the effect of Mimosa pudica root extract on histopathological appearance of Rattus norvegicus brain induced by Naja sputatrix venom. Thirty rats were divided into 5 groups. There were 2 control groups and 3 treatment groups, which was given 250, 500, and 1000 mg/kg BW of Mimosa pudica root extract orally. The first 7 days each group was adapted to the environment. On the 8th day, the treatment was started by injecting Naja sputatrix LD50 (0,13 L/gram BW) IM in gluteus muscle, continued with giving Mimosa pudica root extract orally for the treatment groups 5 minutes after venom injection. 6 hours after the last treatment, rats were killed by cervical dislocation, injected with formalin 10% in the heart, then necropsied. Histopathological evaluation was done to score brain damage based on meningitis, perivascular cuffing, and necrotic cells using HE stain with 1000x magnification. The result showed 1000 mg/kg BW dosage of Mimosa pudica root extract can reduce brain damage based on meningitis, perivascular cuffing, and necrotic cells in Rat (Rattus norvegicus) caused by Naja sputatrix venom and gave significant difference (p < 0.05) among the treatment groups.
The Efficacy of Putrimalu Root Extract (Mimosa pudica) Against Mortality and Histopathological of Rat Muscles Injected Naja sputatrix Venom Rendragraha, Briantono Willy; Legowo, Djoko; Kuncorojakti, Suryo; Chusniati, Sri; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36486

Abstract

The purpose of this experiment was to know the effect of Mimosa pudica root extract (normal water extraction) on mortality and muscle histopatological in experimental animal (Rattus norvegicus) which injected with LD50 of Naja sputatrix venom. Fresh Mimosa pudica root dried without sunshine and ground into coarse powder with steamroller (mash 3mm). Plant extract were prepared by stirring 4 g of the powder into 200 ml of water for 3 hours at room temperature and filtered with muslin cloth before freezdried. 30 rat divided into five groups, P0(C+), P1(C-) P2, P3, and P4. P0 (positive control group) injected by saline intra muscular and given aquadest peroral after five minutes, P1 (negative control) injected with Naja sputatrix venom and given aquadest peroral after five minutes. P2, P3, and P4 injected by Naja sputatrix venom and given Mimosa pudica extract with 250mg/KgBW, 500mg/KgBW, and 1000mg/KgBW doses. All of the experimental animal observed about six hours and than euthanized, musculus gluteus maximus (location of injection) was taken for histopatological examination. In this experiment observed that the normal water extraction of mimosa pudica can't reduce mortality of experimental animals but it can decrease muscle damage in 1000mg/KgBW dose.
Co-Authors Ahmad Saeroji Amaris, Aulia Puspa Amin Pujiati Andriani, Ratna Arimbi Bestari Dwi Handayani Boedi Setiawan Chairul Anwar Charles, Vanessa Ann Chusniati, Sri D.K. Wardani, Lailia Dadik Rahardjo, Dadik Darmawan, Moch. Rizky Eka Pramyrtha Hestianah, Eka Pramyrtha Endang Suprihati Hani Plumeriastuti Hayuning Nurrodhiya Hidajati, Nove Ismiyati, Ismiyati Ismudiono Ismudiono, Ismudiono Kadek Rachmawati Kuncorojakti, Suryo Kurniati, Yuniar Larasati, Galuh Wiedani Kusuma Dyah Lilik Maslachah Mochamad Lazuardi Mufasirin Nusdianto Triakoso Permatasari, Roselia Yuliani Poedji Hastutiek Prakosa, Dwi Untung Pujiyanto, Mahendra Rendragraha, Briantono Willy Rochmah Kurnijasanti Sholikah, Mar'atus Suzanita Utama Tita Damayanti Lestari Tusyanah Tusyanah Tyasningsih, Wiwiek Wahyuni, Retno Sri Xin, Irene Teh Kai Yustinasari, Lita Rakhma Zainal Arifin