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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner journal of Basic Medical Veterinary
Estoepangestie, Agnes Theresia Soelih
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : journal of Basic Medical Veterinary

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Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Mutiarasari, Nonie Olivia Adia; Harijani, Nenny; Rantam, Fedik Abdul; Raharjo, Dadik; Estoepangestie, Agnes Theresia Soelih; Handijatno, Didik
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Co-Authors Adiana Mutamsari Witaningrum Amrullah, Amirul Muslim Benjamin Christoffel Tehupuring, Benjamin Christoffel Didik Handijatno Fedik Abdul Rantam Firdausyi, Aulia Amatul Habibi, Muhammad Afif Hani Plumeriastuti Harijani, Nenny Ira Sari Yudaniayanti Iwan Sahrial Hamid Lucia Tri Suwanti, Lucia Tri Mahdi, Daffa Amanul Mustofa Helmi Effendi Mutiarasari, Nonie Olivia Adia Nursalim, Muhammad Tauhid Nusdianto Triakoso Praja, Ratih Novita Prastiya, Ragil Angga Primarizky, Hardany Purnamasari, Kartika Raharjo, Dadik Saputro, Amung Logam Sardjana, I Komang Wiarsa Sarudji, Suryanie Satria, Rafif Galih Solikhah, Tridiganita Intan Tacharina, Martia Rani Tyasningsih, Wiwiek Wibawati, Prima Ayu Wisnu Nurcahyo