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All Journal Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction

Primarizky, Hardany

Department Of Veterinary Clinics, Faculty Of Veterinary Medicine, Universitas Airlangga, Surabaya

Author-ID : 3558539

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Articles

Prevalence of Blood Protozoa Disease on Cattle and Buffalo in Moyo Hilir Sub-District, Sumbawa District West Nusa Tenggara Melani Anggraini; Hardany Primarizky; Mufasirin Mufasirin; Lucia Tri Suwanti; Poedji Hastutiek; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research was to determine the type and prevalence of blood protozoa disease on cattle and buffalo in Moyo Hilir sub-district, Sumbawa District, Wes Nusa Tenggara. This research was taken on july until December 2018. This research used a non-experimental method through an observation study. The samples of this research were made in thin blood smear using 20% Giemsa’s stain and all samples were from 200 blood of cattle and buffalo in Moyo Hilir sub-district, Sumbawa District, West Nusa Tenggara. The results showed that the prevalence of blood protozoa disease was 11.5 Based on the type of blood protozoa, the highest prevalence was Anaplasma sp. (11%), followed by Theileria sp. (0.5%), while Babesia sp. And Trypanosoma sp. Were not found. Based on the livestock types, the prevalence in buffalo was higher (17%)than cttle (6%). Based on sex the prevalence in female was higher (12.6%) than male (10.11%). Based on age of livestock, the prevalance of livestock with age > 2 years old was higher (13.33%) than livestock with age ≤ 2 years old (10.4%) based on the location of the village, blood protozoa disease were only found in Olatrawa village (17.85%) and Serading village (17.82%), while in Kakiang and Moyo village were not found. The conclusion of this research indicate that prevalence of blood protozoa disease was 11.5% and only Anaplasma sp. And Theileria sp. Were found.

Prevalence of Rumen and Reticulum Trematodes in The Goat Slaughtered at Pegirian Surabaya Slaughter House using Digestive Surgery Method Novia Intan Kurnia; Setiawan Koesdarto; Herry Agoes Hermadi; Kusnoto Kusnoto; Hardany Primarizky; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the kind of trematodes which infected the rumen and reticulum of goat slaughtered at RPH Pegirian Surabaya using digestive surgery method, and determine the prevalence result. 36 samples of rumen and reticulum were collected from RPH Pegirian from September - November 2017 then using digestive surgery to obtain fresh adult worms, afterwards using the Semichen-Acetic Carmine staining method to makes the object easy to identifying. The results showed that species was found on goat was Paramphistomum cervi and Cotylophoron cotylophorum, in the Paramphistomum cervi are several characters wich is oral sucker, genital pore, uterus, testis, ventral sucker and in Cotylophoron cotylophorum contain oral sucker, branched oesophagus, uterus, vitelin glands, testes , posterior sucker. 3 rumen positive trematodosis with prevalence of 8.33% Chi-square statistic test (p>0.05) showed no effect between goat species and Trematodosis prevalence, 20 kacang goat and 16 peranakan etawa goat found 2 positive kacang goat (10%) and 1 peranakan etawa goat positive (6.25%).

Isolasi dan Identifikasi Cemaran Bakteri Escherichia Coli pada Telur Penyu Lekang (Lepidochelys olivacea) yang Gagal Menetas Menetas di Sarang Semi Alami Pantai Boom Banyuwangi Haprabu, Bhima Rochman Satya; Primarizky, Hardany; Wibawati, Prima Ayu; Haditanojo, Wiyanto
Jurnal Medik Veteriner Vol. 1 No. 3 (2018): October
Publisher : Universitas Airlangga

Tujuan dari penelitian ini adalah untuk mengidentifikasi bakteri Escherichia coli dalam telur yang gagal menetas dari penyu Lekang (Lepidoehelys olivacea) di sarang semi-alami, pantai Boom, Banyuwangi. Bakteri diisolasi dari 12 kuning telur penyu yang gagal menetas dan dari induk penyu yang berbeda. Pengambilan sampel dilakukan dengan teknik sampel random berstrata. Kontaminasi E. coli dideteksi dengan menggunakan uji pendugaan, uji konfirmasi, dan uji biokimia IMVIC, berdasarkan Standar Nasional Indonesia 01-2332.1-2015 tentang cara uji mikrobiologi-bagian 1: Penentuan coliform dan E. coli pada produk perikanan. Hasil penelitian ini menunjukkan bahwa sebanyak 3 sampel atau 25% dari total sampel terkontaminasi E. coli, yaitu sampel B1, B3, dan C3. Berdasarkan Most Probability Number (MPN) Jumlah E. coli pada masing masing sampel adalah sampel B1 sejumlah 3 MPN/g, B3 sejumlah 7,2 MPN/g dan C3 sejumlah 9,2 MPN/g. E. coli bukan bakteri utama yang menyebabkan kegagalan penetasan telur penyu di sarang semi-alami, pantai Boom, Banyuwangi. Namun pada pengujian sampel ditemukan bakteri golongan koliform fekal pada sebagian besar sampel yaitu 8 sampel atau 66,67%, koliform non fekal sebanyak 1 sampel atau 8,33% dan non koliform sebanyak 3 sampel atau 25% dari total 12 sampel. Sumber kontaminasi E. coli diduga berasal dari induk penyu, lingkungan sarang semi-alami, dan pekerja Banyuwangi Sea Turtle Foundation.

Efektivitas Ekstrak Daun Afrika (Vernonia amygdalina) Secara Topikal Terhadap Kepadatan Kolagen dalam Penyembuhan Luka Insisi Pada Tikus Putih (Rattus norvegicus) Rachmanita, Rona Taufiqul; Primarizky, Hardany; Setiawan, Boedi; Agustono, Bodhi; Saputro, Amung Logam
Jurnal Medik Veteriner Vol. 2 No. 1 (2019): April
Publisher : Universitas Airlangga

Tujuan dari penelitian ini adalah untuk mengetahui efektivitas pemberian ekstrak daun Afrika (Vernonia amygdalina) secara topikal terhadap kepadatan kolagen dalam penyembuhan luka insisi pada tikus putih (Rattus norvegicus). Dua puluh ekor tikus putih dibagi secara acak ke dalam lima kelompok, yaitu kontrol negatif (K-) yang diberi basis salep, kontrol positif (K+) yang diberi salep povidone iodine 10%, kelompok perlakuan (P1, P2, P3) yang diberi perlakuan dengan salep ekstrak daun Afrika 16,8%, 28%, dan 39,2%. Perlakuan diberikan secara topikal pada lokasi luka insisi sebanyak dua kali sehari selama empat belas hari. pada hari keempat belas tikus dieuthanasi dan daerah insisi dieksisi untuk preparasi histopatologi. Data yang didapatkan dianalisis menggunakan Kruskal-Wallis dengan SPSS 20. Hasil uji menunjukkan p=0,077 yang berarti tidak terdapat perbedaan yang nyata (p>0,05) antara keseluruhan perlakuan. Berdasarkan hasil tersebut rata-rata P1 menunjukkan hasil skoring kepadatan kolagen tertinggi dibandingkan perlakuan yang lain. Kesimpulan penelitian ini yaitu bahwa daun Afrika (Vernonia amygdalina) tidak efektif meningkatkan kepadatan kolagen dalam penyembuhan luka insisi pada tikus putih (Rattus norvegicus).

Risk Factors and Scabious Prevalence in Cats During 2020 in Griya Satwa Clinic, Magetan Cahya, Nedriana; Primarizky, Hardany; Yunita, Maya Nurwartanti
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.81-86

Scabious is a disease that often infests cats, originating from the family Sarcoptidae. This study aimed to determine the prevalence and factors that can affect the risk of scabious in cats. The study was evaluated at the Griya Satwa Clinic, Magetan in January-February 2021. The design of this study was a retrospective cohort study by taking secondary data during the 2020 period as study material. The data obtained were then entered into the Statistical Package for the Social Sciences (SPSS) version 20 for windows to analyze the association's relationship and increased risk by calculating the Chi-Square and Odds Ratio. The samples obtained were 76 cats infested with scabious. The analysis of the association of risk factors for scabious was breed (P = 0.428), age (P = 0.793), sex (P = 0.426). The results of the prevalence of scabious in cats from the study during January-February 2021 in the 2020 period were 9.5%, and the analysis of the association (Chi-Square) and the increased (Odds Ratio) of factors including breed, age, sex on the risk of scabious in cats were not significance / absent influence, The probability of error obtained is outside the tolerance set by the researcher, so the results are not significant (p>0,05).

An Evaluation of Nutritional Factors on The Prevalence of Urolithiasis in Cats at The Veterinary Teaching Hospital, Universitas Airlangga During 2017-2019 Mahdi, Daffa Amanul; Sardjana, I Komang Wiarsa; Yudaniayanti, Ira Sari; Estoepangestie, Agnes Theresia Soelih; Triakoso, Nusdianto; Primarizky, Hardany
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.180-184

Stones, or uroliths, in the urinary tract cause a disorder called urolithiasis. Patients with urolithiasis may experience blockage and possibly mortality; therefore, prompt treatment is imperative. This study aimed to investigate the prevalence of urolithiasis and its nutritional association in cats treated at the Veterinary Teaching Hospital, Universitas Airlangga during 2017-2019. Data was collected from the secondary resource from the medical report and the factor of feed nutrition involved fiber, protein, fat, and moisture. Data was analyzed using logistic regression to determine the influence of nutrition on the prevalence of urolithiasis in cats in the respective period. The findings indicated that in 2017, urolithiasis prevalence was 0,96%; in 2018, it was 5,55%; and in 2019, it was 0,91%. Meanwhile, it was revealed that the prevalence of urolithiasis was not significantly impacted by feed nutritional variables.

The Effect of Apis dorsata Honey Treatment on Calcium Levels of Mandibular Bone in Ovariohysterectomized Wistar Rats Rohmah, Siti Nur; Yudaniayanti, Ira Sari; Primarizky, Hardany; Hastutiek, Poedji
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.1-7

This study aimed to evaluate the efficacy of Apis dorsata honey on calcium levels in the mandibular bone of Wistar rats after ovariohysterectomy as an animal osteoporosis model for treating cases of osteoporosis. This study used 20 female rats, which were divided into five treatment groups with four replications. SHAM was the negative control group; OH was the ovariohysterectomized group without treatment as a positive control; the AD1, AD2, and AD3 groups were ovariohysterectomized rats with A. dorsata honey at doses of 1 g/kg bw, 2 g/kg bw, and 4 g/kg bw for 84 days. After 84 days of treatment, the left mandibular bones of the rats were collected, weighed, and stored in 10% neutral buffered formalin. Calcium levels were calculated using proximate analysis. The results showed a decrease in bone calcium levels in the ovariohysterectomy group, the highest results were in the SHAM group, and the lowest results were in the AD3 group. It can be concluded that the administration of A. dorsata honey could not maintain the calcium level of the mandibular bone in the ovariohysterectomized Wistar rats.

White guava (Psidium guajava L.) fruit juice ameliorated the number of spermatogenic cells in rats (Rattus Novergicus) exposed to lead acetate Muhammad Faizal Adiutomo; Suzanita Utama; Herry Agoes Hermadi; Wurlina Wurlina; Hardany Primarizky; Rochmah Kurnijasanti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.39-46

This study aims to determine the effect of oral administration of white guava fruit juice (Psidium guajava L.) on the spermatogenic cells of rats (Rattus norvegicus) exposed orally to lead acetate. Twenty-five male rats (Rattus norvegicus) of the Wistar strain were randomly divided into five groups. Placebo group rats (P0) were given 0.5 mL distilled water twice every day orally at four-hour intervals. Groups T0, T1, T2, and T3 were given lead acetate 50 mg/kg bw orally, then respectively given distilled water, 100, 50 and 25% (v/v) white guava (Psidium guajava L.) fruit juice orally four hours later for 14 days. On day-15, the rats were sacrificed and their testicles were taken for histological preparations. Data were analyzed using Analysis of Variance followed by Duncan's Multiple Range Test. The results showed that the number of spermatogonia, spermatocytes and spermatids cells of rats in T0 group was lower (p <0.05) than in P0 group. Higher spermatogonia, spermatocytes and spermatids cells (p <0.05) were found in the T1 group compared to the T0 group. However, the number of spermatogonia and spermatocytes in the T1 group was still lower than in the P0 group. The number of spermatids in the T3 group was almost the same (p >0.05) as in the P0 group. This study found that white guava pure juice without dilution was the best for maintaining the number of spermatids, but was unable to restore the number of spermatogonia and spermatocyte cells in rats exposed to lead acetate.

Moringa oleifera leaf extract restored the diameter and epithelium thickness of the seminiferous tubules of rat (Rattus norvegicus) injected with gentamicin Mardatillah, Meidy; Wurlina, Wurlina; Yudaniayanti, Ira Sari; Primarizky, Hardany; Plumeriastuti, Hani; Hamid, Iwan Sahrial
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 1 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i1.2022.15-21

This research aims to determine the effect of Moringa oleifera leaf extract (MLE) on the diameter and epithelium thickness of the seminiferous tubules of rats induced with gentamicin. This study used 25 male rats (Rattus norvegicus) aged 8-12 weeks with 150-200 grams body weight. Negative control group (C-) was injected with aquadest and given 0.5% Na-CMC orally, positive control group (C+) was induced with gentamicin injection of 5 mg/kg BW/day and given 0.5% Na-CMC orally, while groups T1, T2, and T3 were induced with gentamicin at the same dose and treated orally with MLE of 200, 316, 500 mg/kg BW/day. Analysis of variance showed significant differences and further analysis using Duncan Multiple Range Test showed difference in each group. The results showed that treatment with MLE of 500 mg/kg BW/day improved the diameter and epithelium thickness of the seminiferous tubules of rats injected with gentamicin. It could be concluded that MLE restored the diameter and epithelium thickness of the seminiferous tubules of rats induced with gentamicin.

Co-Authors Agustono, Bodhi Boedi Setiawan Cahya, Nedriana Estoepangestie, Agnes Theresia Soelih Haditanojo, Wiyanto Hani Plumeriastuti Haprabu, Bhima Rochman Satya Herry Agoes Hermadi Ira Sari Yudaniayanti Iwan Sahrial Hamid Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Mahdi, Daffa Amanul Mardatillah, Meidy Melani Anggraini Mufasirin Muhammad Faizal Adiutomo Novia Intan Kurnia Nusdianto Triakoso Poedji Hastutiek Rachmanita, Rona Taufiqul Rochmah Kurnijasanti Rohmah, Siti Nur Saputro, Amung Logam Sardjana, I Komang Wiarsa Setiawan Koesdarto Sunarso, Agus Suzanita Utama Wibawati, Prima Ayu Wurlina, W Yunita, Maya Nurwartanti

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