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All Journal Jurnal Teknologi Laboratorium Jurnal Medik Veteriner journal of Basic Medical Veterinary Jurnal Agro Veteriner Genbinesia Journal of Biology
Tacharina, Martia Rani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Journal : journal of Basic Medical Veterinary

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Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amrullah, Amirul Muslim; Rantam, Fedik Abdul; Raharjo, Dadik; Tyasningsih, Wiwiek; Estoepangestie, Agnes Theresia Soelih; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine's Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro Saraswati, Afif Tasya; Sugihartuti, Rahmi; Puspitasari, Yulianna; Witaningrum, Adiana Mutamsari; Rachmawati, Kadek; Maslachah, Lilik; Raharjo, Hartanto Mulyo; Hisyam, Mirza Atikah Madarina; Tacharina, Martia Rani
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.51106

Abstract

This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.
Co-Authors Adi Sofyan Ansori, Muhammad Adiana Mutamsari Witaningrum Amaq Fadholly Amrullah, Amirul Muslim Arif Nur Muhammad Ansori Astuti, Elita Sriwidya Dhea Kharisma, Viol Dings, Tim Godefridus Antonius Estoepangestie, Agnes Theresia Soelih Fedik Abdul Rantam Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Kadek Rachmawati Kharisma, Viol Dhea Lilik Maslachah Mirino, Mario Navyseal Mustofa Helmi Effendi Rahadian Zainul Raharjo, Dadik Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmi Sugihartuti, Rahmi Rasyadan Taufiq Probojati Sabdoningrum, Emy Koestanti Saraswati, Afif Tasya Suwarno Suwarno Turista, Dora Dayu Rahma Tyasningsih, Wiwiek Ullah, Md. Emdad Widya Paramita Lokapirnasari Wudhu, R Zaksara Fero Ali Mutaqin Yulanda Antonius Yulianna Puspitasari